• Molecules and Cells
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• v.45 no.12
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• pp.896-910
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• 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly transmissible and potentially fatal virus. So far, most comprehensive analyses encompassing clinical and transcriptional manifestation have concentrated on the lungs. Here, we confirmed evident signs of viral infection in the lungs and spleen of SARS-CoV-2-infected K18-hACE2 mice, which replicate the phenotype and infection symptoms in hospitalized humans. Seven days post viral detection in organs, infected mice showed decreased vital signs, leading to death. Bronchopneumonia due to infiltration of leukocytes in the lungs and reduction in the spleen lymphocyte region were observed. Transcriptome profiling implicated the meticulous regulation of distress and recovery from cytokine-mediated immunity by distinct immune cell types in a time-dependent manner. In lungs, the chemokine-driven response to viral invasion was highly elevated at 2 days post infection (dpi). In late infection, diseased lungs, post the innate immune process, showed recovery signs. The spleen established an even more immediate line of defense than the lungs, and the cytokine expression profile dropped at 7 dpi. At 5 dpi, spleen samples diverged into two distinct groups with different transcriptome profile and pathophysiology. Inhibition of consecutive host cell viral entry and massive immunoglobulin production and proteolysis inhibition seemed that one group endeavored to survive, while the other group struggled with developmental regeneration against consistent viral intrusion through the replication cycle. Our results may contribute to improved understanding of the longitudinal response to viral infection and development of potential therapeutics for hospitalized patients affected by SARS-CoV-2.

• Journal of Practical Agriculture & Fisheries Research
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• v.11 no.1
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• pp.79-94
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• 2009

The research project was conducted to develop a paper bagging machine for grape. This technology was aimed to highly reduce a labor for paper bagging in grape and bakery. In agriculture labor and farm population has rapidly decreased since 1980 in Korea so there was so limit in labor. In particular there is highly population in women and old age at rural area and thus labor cost is so high. Therefore a labor saving technology in agricultural sector might be needed to be replaced these old age with mechanical and labor saving tool in agriculture. The following was summarized of the research results for development of a paper bagging machine for grape. 1. Development of a new paper bagging machine for grape - This machine was designed by CATIA VI2/AUTO CAD2000 programme. - A paper bagging machine was mechanically binded a paper bag of grape which should be light and small size. This machine would be designed for women and old age with convenience during bagging work at the field site. - This machine was manufactured with total weight of less than 350g. - An overage bagging operation was more than 99% at the actual field process. - A paper bagging machine was designed with cartridge type which would be easily operated between rows and grape branches under field condition. - The type of cartridge pin was designed as a C-ring type with the length of 500mm which was good for bagging both grape and bakery. - In particular this machine was developed to easily operated among vines of the grape trees. 2. Field trials of a paper bagging machine in grape - There was high in grape quality as compared to the untreated control at the application of paper bagging machine. - The efficiency of paper bagging machine was 102% which was alternative tool for the conventional. - The roll pin of paper bagging machine was good with 5.3cm in terms of bagging precision. - There was no in grape quality between the paper bagging machine and the conventional method. - Disease infection and grape break was not in difference both treatments.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.2
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• pp.191-197
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• 1984

This study was conducted to investigate the flowering habit for the botanical types of peanut (Arachis hypogaea. L) in cultural limiting region. Eight peanut varieties pertaining to virginia, Spanish, Valencia, Shinpung type were used in this study. Shinpung type, a new erect type was developed at Crop Experiment Station, ORD. Appearance of the first flower of peanut plants is observed at a relatively early growing stage. Number of days to first flower were 41 days under vinyl-mulching while non-mulching culture took 55 days. Number of days to first flower were delayed in order of Valencia, Spanish, Shinpung, Virginia type. Flowering durations were distinguished among botanical types and days of those were Virginia 85-93, Spanish 101-105, Valencia 106-113, Shinpung 82-88 days, respectively. The Spanish and Valencia types produced more flowers than Virginia and Shinpung types. The maximum flowering stage of Shinpung and Virginia types reached early and ceased by one cycle, while Spanish and Valencia types came late and showed maximum stage through the early and late stage during the growth stage. By vinyl-mulching, the maximum flowering stage was shortened by 20 days than that of non-mulching. Distribution of flowers for each of branch positions to total flowers occupied 52-53% in two cotyledonary branchs, 23-27% in 3rd to 5th branchs, 20-25% in over. 6th branchs. Shinpung type was considered more favorable botanical type than the other types for the breeding of early maturing high yielding variety because of shortening of days to first flower, flowering duration and distribution of lower branch of effective flowers.

• Korean Journal of Animal Reproduction
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• v.24 no.4
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• pp.365-373
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• 2000

The purpose of this study was to investigate the effects of the fusion pulses and fusion media on fusion rate and the development of embryos produced by somatic cell nuclear transfer in Hanwoo (Korean cattle). Nuclear donor cumulus and fetal fibroblast cells were cultured in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum at 38.5$^{\circ}C$ in a humidified atmosphere of 5% $CO_2$in air. The in vitro matured oocytes were enucleated and then the isolated donor cells were introduced. The cumulus cell and cytoplast were fused using one pulse of 70 volts for 40$mutextrm{s}$, two pulses of 70 volts for 40$mutextrm{s}$ and one pulse of 180 volts for 15$mutextrm{s}$. The fetal fibroblast cell and cytoplast were fused using one pulse of 180 volts for 15$mutextrm{s}$ or 30$mutextrm{s}$. The cumulus cell and cytoplast were fused using mannitol and Zimmerman cell fusion medium (ZCFM) as a fusion medium. The fused embryos were activated after the fusion with 10 $\mu$M calcium ionophore for 5 min and 2 mM 6-dimethyl- aminopurine for 3 h. The nuclear transfer embryos were cultured in 500 ${mu}ell$ well of modified CR1aa supplemented with 3 mg/$m\ell$ BSA in th $\varepsilon$ four well dish cove red with mineral oil. After 3 days culture, culture medium was changed into modified CRlaa medium containing 1.5 mg/$m\ell$ BSA and 5% FBS for 4 days. The incubation environment was 5% $CO_2$, 5% $O_2$, 90% $N_2$ at 38.5$^{\circ}C$. When the cumulus cells were fused with enucleated oocytes by three different fusion pulses, one pulse of 180 volts for 15 $mutextrm{s}$ yielded the highest fusion rate and developmental rate to blastocyst among the pulses (P＜0.05). When the fetal fibroblast cells were fused with enucleated oocytes, one pulse of 180 volts for 30$mutextrm{s}$ yielded significantly higher fusion rate compared with that for 15 $mutextrm{s}$(P＜0.05). The present result indicates that the fusion rate between karyoplast and cytoplast was affected by the cell type and the optimal fusion condition was different according to cell type or size. When the fusion was conducted by the use of mannitol and ZCFM, the fusion rate was 71.2% and 65.8%, respectively. The developmental rates to blastocyst were 37.8% and 39.8%, respectively. There was no significant difference between two fusion media in the developmental rate of cumulus cell nuclear transfer embryos. These results indicate that optimal electric current should be selected according to cell type.

• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.1
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• pp.49-59
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• 2019

The field trial was performed to evaluate the rice growth and yield in different direct seeding methods after Italian Ryegrass Harvest The required time for seed emergence was for 7 ~ 8days in the tested direct seeding methods and there was high in seedling establishment in order of wet hill-seeding with iron-coated seeds > water seeding with iron-coated seeds > wet hill-seeding with soil coverage with pregerminated seeds. The rice plant height was shorter in the tested direct seeding methods than that of machine transplanting until 45day after seeding but there was not significant difference in terms of statistical analysis at 63day after seeding. The growth of tiller number in the rice plant was high in water seeding with iron-coated seeds and wet hill-seeding with soil coverage and low in wet hill-seeding with iron-coated seeds compared to machine transplanting. The yield component in the tested direct seeding methods was not significant difference in terms of statistical analysis. The milled rice yield in the tested direct seeding methods was higher 2 ~ 8% being with 4.94 ~ 5.24t/ha than that of machine transplanting but there was not significant difference in terms of statistical analysis. The percentage of head rice was low in the tested direct seeding methods compared to machine transplanting. The weedy rice was not occurred in the tested rice cultivation methods. In conclusion the direct seeding method would be recommended to be a suitable to in following by Italian ryegrass harvesting in southern area of Korea in terms of reduction in production cost and high income basis for rice growing farmers.

• Journal of Practical Agriculture & Fisheries Research
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• v.20 no.1
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• pp.5-18
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• 2018

The field trial was performed to evaluate the rice growth and yield in direct seeding cultivation with iron-coated rice seeds. The required time for seed emergence was for 9~11days in the tested direct seeding methods. That was 1~2days earlier in direct seeding with pregerminated seeds than that of direct seeding with iron-coated seeds. The seedling establishment was highest in water seeding with iron-coated seeds but there was not significant difference in terms of statistical analysis. The rice plant height was taller in water seeding with broadcasting method than that of wet hill-seeding methods and in direct seeding with iron-coated seeds than that of direct seeding with pregerminated seeds. The tiller number in the rice plant was the highest in machine transplanting at 30days after direct seeding(June 17) and in water seeding with iron-coated seeds at 45days after seeding(DAS) and 60DAS. The tiller number of 75 and 90DAS in the tested rice cultivation methods being with 352~405/m² was not significantly different in terms of statistical analysis. The heading time was not different in rice direct seeding methods but 2 day earlier in direct seeding with iron-coated seeds than that of direct seeding with pregerminated seeds. The culm length was the highest in water seeding with iron-coated seeds and the panicle length was the longest in wet hill-seeding with pregerminated seeds. The panicle number per m² was highest in water seeding with iron-coated seeds but not significant difference among the tested rice cultivation methods. The water seeding with iron-coated seeds resulted in the highest spikelet number per m² and the heaviest grain weight of brown rice. Percentage of ripened kernel was the highest in wet hill-seeding with iron-coated seeds. But there were not significant among the tested rice cultivation methods. The milled rice yield in direct seeding methods was 3~21% higher than that in machine transplanting. Water seeding with iron-coated seeds recorded the highest milled rice yield being with 6.86t/ha.The occurrence of sheath blight was high according to machine transplanting>wet hill-seeding>water seeding. Weed occurrence was the highest in water seeding with pregerminated seeds. Weedy rice occurred not in machine transplanting but occured 0.6~0.7% in direct seeding methods with pregerminated seeds and 0.1% in direct seeding with iron-coated seeds.

• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.1
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• pp.61-70
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• 2019

"Jungmo2510", a rye cultivar, Secale cereal L., was developed by National Institute of Crop Science, RDA in 2015. It was developed from open pollination from within 10 rye cultivars or lines including "Chochun" in 1995. The line "SR95POP-S1-523-1-5-5-4-7-3-B-16-3-19" was selected for its excellent agronomic appearance and was placed in yield trials for two years from 2011 to 2012. The line was designated "Homil55" and was placed in regional yield trials at the four locations around Korea from 2013 to 2015, during which time the name "Jungmo2510" was given. This cultivar is an erect plant type and leaves of short and broad size with a green color, a yellow colored, medium-diameter culm, and a yellowish brown-colored, medium-size grain. The heading date of "Jungmo2510" was April 16, which were 2 days earlier than that of "Gogu". "Jungmo2510" also showed similar to winter hardiness and greater resistance to lodging compared to those of the check cultivar. Over three years, the average dry matter yield of "Jungmo2510" was 802 kg 10a^-1 , which was harvested in late April and was lower than that of the check cultivar "Gogu" (825 kg). The seed productivity of "Jungmo2510" was approximately 481 kg 10a^-1 , which was 2.4% less than that of the check. "Jungmo2510" was higher to than "Gogu" in term of protein content (9.1% and 8.0%, respectively), total digestible nutrients(TDN)(57.5% and 55.5%, respectively), and TDN yield 10a^-1(419 kg and 392 kg, respectively). This cultivar is recommended as a fall sowing crop in areas where the average daily minimum-mean temperatures are higher than -12 ℃ in January, and as a winter crop for whole-crop forage before the planting of rice or green manure around Korea.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.103-103
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• 2022

다복찰은 전라북도농업 기술원에서 2012~2020년에 신명흑찰과 익산488호를 교배하여 육성한 찰벼이며, 중대립, 단간, 내도복, 다수성의 특징을 보인다. 동진찰은 1998년도에 육성되었으며, 우리나라에서 가장 인기 있는 찰벼이다(재배면적 25,161ha/2021년 기준). 드문모심기 재배 기술은 2017년부터 도입되었으며, 밀파 육묘하여 재식밀도 및 재식주수를 감소함으로써 소요되는 재료비 등 경비 및 투입되는 노동력을 절감하는 경제성 있는 기술이다. 따라서 본 연구는 전라북도농업기술원에서 육성한 다복찰 보급 확대 및 가장 인기 높은 동진찰의 드문모심기 재배기술 확립 일환으로 시작되었으며, 2024년까지 3년간 실시할 계획이다. 시험은 기술원 논 포장에서 실시하였으며, 시험품종은 다복찰, 동진찰, 시험요인은 이앙시기(3처리) 및 재식밀도(4처리)를 두었다. 이앙시기별로 이앙 17일 전에 온탕소독(62℃, 10분), 종자소독(32℃, 1일), 침종(32℃, 1일), 간이출하(30℃, 3일) 과정을 거쳐 부직포 육묘(12일 정도)를 실시하였다. 5월20일(이앙520)부터 5월30일(이앙530), 6월9일(이앙609)까지 10일 간격으로 드문모심기 전용 이앙기로 이앙하였으며, 주당 본수는 5.7개 정도였다. 이앙 시기별로 시험포장을 구분하였고 포장내에 2품종, 각각 3.3m2당 80주, 60주, 50주, 37주 등 재식밀도 처리구를 두었으며, 처리구마다 3반복 조사구(10주/반복, 5본/주) 설치하였다. 10a당 9-4.5-5.7kg(N-P₂O₅-K₂O)를 밑거름(50%)-분얼거름(20)-이삭거름(30) 등 3회 거쳐 시비하였다. 중간물떼기는 이앙32일째부터 10일간 실시하였으며, 예상 출수 30일 전에 충분하게 담수하였다. 이앙20일째부터 10일 간격으로 경수, 초장, 엽색도 등 생육 조사, 그리고 출수기, 후기 생육 및 병해충을 조사하였다. 향후 수확기에 수량, 수량구성요소, 미질 및 품위를 분석할 계획이다. 시험토양은 pH 6.0~6.3, EC는0.68~0.85dS/m, 유기물함량은 52~57g/kg 수준으로 높았다. 동진찰 발아율(94.0~98.1%)이 다복찰(89.9~94.9%)보다 우수하였다(3~6%P ↑). 다복찰 묘 충실도(102~106mg/주)가 동진찰(79~101mg/주)보다 다소 좋았으며, 이앙530 묘소질이 가장 좋았다. 동진찰 초장은 다복찰에 비해 다소 길었으나 이앙시기 및 재식밀도별 초장의 변화 유형은 비슷하였다. 이앙520구의 80주에서 초장이 가장 작았고 이앙609구에서는 80주에서 다소 길었으나, 처리구간의 유의성은 없었다. 동진찰 및 다복찰 경수는 37주에서 이앙 후 40일째, 50주, 60주, 80주에서는 30일째 가장 컸다. 출수기는 다복찰에 비해 동진찰이 3~7일 정도 빨랐으며, 특히 이앙520 동진찰에서는 재식밀도가 높을수록 출수기가 빠른 경향을 보였으며, 이앙530 이후에는 재식밀도간 차이가 거의 없었다. 동진찰에 비해 다복찰 간장이 3~6cm 작았으며, 2품종 모두 이앙609구에서 가장 낮은 값을 보였다. 수장도 간장과 비슷한 경향을 보였다. 수수는 2품종 모두 이앙이 늦을수록 증가하였으며, 37주에서 가장 높았으며, 80주에 비해 다복찰, 동진찰 각각 73.8%, 77.4% 높았다. 다복찰의 경우 3.3m²당 수수는 이앙시기별과 관계없이 상대적으로 80주에서 가장 많았고 이앙520에서 재식밀도간 차이는 감소하였지만 이앙시기가 늦을수록 수수는 증가하는 경향을 보였다. 동진찰도 비슷한 경향을 보였으며, 이앙520과 이앙609 사이에 수수 차이는 적었다. 병 발생은 잎집무늬마름병, 세균성벼알마름병, 이삭누룩병이 주로 관찰되었으며, 세균성 벼알마름병은 출수기와 맞물린 이앙530에서 가장 크게 발생하였으며, 이앙520도 비슷한 경향을 보였다. 이삭누룩병 발생이 심하였는데 재식밀도간 차이보다는 이앙시기별 차이가 더 크게 발생하였으며 품종 간의 병 발생 차이는 없었다. 이상의 결과로 수수 측면에서 조기 이앙할 경우에는 50~60주, 늦은 이앙 시에는 80주가 유리할 것으로 보이며, 추후주당 립수, 등숙률, 천립중 등을 조사하고 경영비 등을 고려하여 합리적인 이앙시기 및 재식밀도를 판단할 수 있을 것으로 판단된다.

• Annals of Hepato-Biliary-Pancreatic Surgery
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• v.27 no.4
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• pp.403-414
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• 2023

Backgrounds/Aims: Pancreatoduodenectomy (PD) is recommended in fit patients with a carcinoma (PDAC) of the pancreatic head, and a delayed resection may affect survival. This study aimed to correlate the time from staging to PD with long-term survival, and study the impact of preoperative investigations (if any) on the timing of surgery. Methods: Data were extracted from the Recurrence After Whipple's (RAW) study, a multicentre retrospective study of PD outcomes. Only PDAC patients who underwent an upfront resection were included. Patients who received neoadjuvant chemo-/radiotherapy were excluded. Group A (PD within 28 days of most recent preoperative computed tomography [CT]) was compared to group B (> 28 days). Results: A total of 595 patents were included. Compared to group A (median CT-PD time: 12.5 days, interquartile range: 6-21), group B (49 days, 39-64.5) had similar one-year survival (73% vs. 75%, p = 0.6), five-year survival (23% vs. 21%, p = 0.6) and median time-to-death (17 vs. 18 months, p = 0.8). Staging laparoscopy (43 vs. 29.5 days, p = 0.009) and preoperative biliary stenting (39 vs. 20 days, p < 0.001) were associated with a delay to PD, but magnetic resonance imaging (32 vs. 32 days, p = 0.5), positron emission tomography (40 vs. 31 days, p > 0.99) and endoscopic ultrasonography (28 vs. 32 days, p > 0.99) were not. Conclusions: Although a treatment delay may give rise to patient anxiety, our findings would suggest this does not correlate with worse survival. A delay may be necessary to obtain further information and minimize the number of PD patients diagnosed with early disease recurrence.

• Annals of Hepato-Biliary-Pancreatic Surgery
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• v.28 no.1
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• pp.70-79
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• 2024

Backgrounds/Aims: After pancreatoduodenectomy (PD), an early oral diet is recommended; however, the postoperative nutritional management of PD patients is known to be highly variable, with some centers still routinely providing parenteral nutrition (PN). Some patients who receive PN experience clinically significant complications, underscoring its judicious use. Using a large cohort, this study aimed to determine the proportion of PD patients who received postoperative nutritional support (NS), describe the nature of this support, and investigate whether receiving PN correlated with adverse perioperative outcomes. Methods: Data were extracted from the Recurrence After Whipple's study, a retrospective multicenter study of PD outcomes. Results: In total, 1,323 patients (89%) had data on their postoperative NS status available. Of these, 45% received postoperative NS, which was "enteral only," "parenteral only," and "enteral and parenteral" in 44%, 35%, and 21% of cases, respectively. Body mass index < 18.5 kg/m² (p = 0.03), absence of preoperative biliary stenting (p = 0.009), and serum albumin < 36 g/L (p = 0.009) all correlated with receiving postoperative NS. Among those who did not develop a serious postoperative complication, i.e., those who had a relatively uneventful recovery, 20% received PN. Conclusions: A considerable number of patients who had an uneventful recovery received PN. PN is not without risk, and should be reserved for those who are unable to take an oral diet. PD patients should undergo pre- and postoperative assessment by nutrition professionals to ensure they are managed appropriately, and to optimize perioperative outcomes.