• Korean Journal of Plant Tissue Culture
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• v.27 no.3
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• pp.163-167
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• 2000

This study was carried out to identify varietal difference and the inheritance of the ability of plant regeneration in anther culture of rice. The anthers of 33 Japonica, 11 Indica/Japonica, and 3 Indica rice cultivars were culture on $N_6-Y$_1$$ medium with 1 mg/L NAA and 2 mg/L kinetin. The remarkable variability in plant regeneration were observed among the genotypes, ranging from 0.0% to 19.4% Most of Japonica rices were resulted to have better culturability than that of Indica/Japonica and Indica type cultivars. Newly developed Japonica rices, 'Ilmibyeo' and 'Hangnambyeo' showed to have regenerability with the frequency of 19.4% and 18.1% respectively. The segregation mode for callus formation and plant regenerationin anther culture of F$_2$ population of 'Milyang 23/Chucheongbyeo' showed a continuous variation. The variation of plant regeneration frequency in anther culture of F$_2$ plants ranged from 0% to 33.3% with a mean of 6.3% The broad-sense heritability estimated from the ability of plant regeneration was considerably high (82.7%).

• Journal of Korean Society of Forest Science
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• v.102 no.4
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• pp.560-565
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• 2013

Population genetic structure and diversity of Ulmus davidiana var. japonica in South Korea were studied using ISSR markers. A total of 45 polymorphic ISSR amplicons were cropped from 7 ISSR primers and 171 individuals of 7 populations. The average of effective alleles and the proportion of polymorphic loci were 1.5 and 89% respectively. The Shannon's diversity index (I) was 0.435 and the expected heterozygosity from the frequentist's method ($H_e$) and the Bayesian inference (hs) were 0.289 and 0.323 respectively. From AMOVA, 4.2% of total genetic variation in the elm populations was explained with the difference among populations (${\Phi}_{ST}=0.042$) and the other 95.8% was distributed within populations. The ${\theta}^{II}$ value by Bayesian method which was comparable to the FST was 0.043. So the level of genetic diversity in the elm populations was similar to that in Genus Ulmus and the level of genetic differentiation was lower than that of others. No population showed a significant difference in the population-specific fixation indices (average of $PS-F_{IS}=0.822$) or the population-specific genetic differentiations (average of $PS-F_{ST}=0.101$). Seven populations were allocated into 3 groups in the UPGMA and the PCA, but the grouping patterns were different. Also, we could not confirm any geographic trend from Bayesian clustering.

• Korean journal of applied entomology
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• v.37 no.1
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• pp.19-22
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• 1998

Number of loci, allele frequencies, and subunit structures of 17 kinds of isozymes were analyzed in a laboratory strain of the beet armyworm, Spodoptera exigua (Hubner) to get genetic markers. These isozymes had 30 loci with 21 polymorphic (70.0% polymorphism); effective number of alleles per locus, average heterozygosity (H,), and inbreeding coefficient (F) were 2.52, 32.8%, and 2 1.0%, respectively.

• Animal cells and systems
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• v.9 no.1
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• pp.27-35
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• 2005

Bupleurum euphorbioides is isolated and restricted to high mountains in Korea northeastern China. Its conservation depends on whether it is threatened by inbreeding or a loss of genetic diversity. We compared the genetic variability in B. euphorbioides with B. longiradiatum, a widespread congener, to understand how they differ in their population genetic structure. Although B. euphorbioides showed a little lower genetic variability than B. longiradiatum, $F_{IS}$ statistics for most loci were strongly positive in both B. euphorbioides (0.445) and B. longiradiatum (0.553). In addition, B. euphorbioides showed higher mean $F_{ST}$ value than B. longiradiatum (0.297 vs 0.194). It might be due to the polycarpic nature of B. longiradiatum, which holds higher genetic potentials effectively in homogeneous environment than the monocarpic B. euphorbioides. The results suggested that B. euphorbioides is a genetically viable species, and that they are threatened primarily by environmental factor.

• The Korean Journal of Nuclear Medicine Technology
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• v.17 no.2
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• pp.59-66
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• 2013

Purpose: Recently, there is an increase of the number of hospitals using auto dispenser to reduce occupational radiation exposure when drawing up of the $^{18}F-FDG$ dose (5.18 MBq/kg) in a syringe from the dramatic high activity of $^{18}F-FDG$ multidose vial. The aim of this study is to confirm that using auto dispenser actually reduces the radiation exposure for technologists. Also we analyzed the reproducibility of auto dispenser to find optimized dispensing method for the device. Materials and Methods: We conducted three experiments. Comparison of radiation exposure on chest and hands: The chest and hands exposure dose received by technologists during the injection were measured by electronic personal dosimeter (EPD) and ring TLD respectively. Reproducibility of dispensed volume: We draw up the normal saline into 5 and 2 mL syringe using auto dispenser by changing the volume from 1 to 15 mm for 5 times in the same setting of the volume. The weight of 5 normal saline dispensed from the device at same volume was measured using micro balance and calculated standard deviation and coefficient of variation. Reproducibility of dispensed radioactivity: We dispensed 362.6 $MBq{\pm}10%$ of $^{18}F-FDG$ in 5 and 2 mL syringes from the multidose vial of different specific activity. In the same setting of volume, we repeated dispensing for 4 times and compared standard deviation and coefficient of variation of radioactivity between 5 syringes. Results: There was significant difference in the average of chest exposure dose according to the dispensing methods (P<0.05). Also, when dispensing $^{18}F-FDG$ in manual method, exposure dose was 11.5 times higher in right hand and 4.8 times higher in left hand than in auto method. In the result of reproducibility of dispensed volume, standard deviation and coefficient of variation shows decline as the dispensing volume increases. As a result of reproducibility of dispensed radioactivity, standard deviation and coefficient of variation increases as the specific activity increases. Conclusion: We approved that the occupational radiation exposure dose of technologists were reduced when dispensing $^{18}F-FDG$ using auto dose dispenser. Secondly, using small syringes helps to increase reproducibility of auto dose dispense. And also, if you lower the specific activity of $^{18}F-FDG$ in multidose vial below 915-1,020 MBq/mL, you can use auto dispenser more effectively keeping the coefficient of variation lower than 10%.

• Bulletin of the Korean Mathematical Society
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• v.53 no.5
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• pp.1531-1548
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• 2016

Let C[0, t] denote the space of real-valued continuous functions on [0, t] and define a random vector $Z_n:C[0,t]{\rightarrow}\mathbb{R}^n$ by $Z_n(x)=(\int_{0}^{t_1}h(s)dx(s),{\ldots},\int_{0}^{t_n}h(s)dx(s))$, where 0 < $t_1$ < ${\cdots}$ < $ t_n=t$ is a partition of [0, t] and $h{\in}L_2[0,t]$ with $h{\neq}0$ a.e. Using a simple formula for a conditional expectation on C[0, t] with $Z_n$, we evaluate a generalized analytic conditional Wiener integral of the function $G_r(x)=F(x){\Psi}(\int_{0}^{t}v_1(s)dx(s),{\ldots},\int_{0}^{t}v_r(s)dx(s))$ for F in a Banach algebra and for ${\Psi}=f+{\phi}$ which need not be bounded or continuous, where $f{\in}L_p(\mathbb{R}^r)(1{\leq}p{\leq}{\infty})$, {$v_1,{\ldots},v_r$} is an orthonormal subset of $L_2[0,t]$ and ${\phi}$ is the Fourier transform of a measure of bounded variation over $\mathbb{R}^r$. Finally we establish various change of scale transformations for the generalized analytic conditional Wiener integrals of $G_r$ with the conditioning function $Z_n$.

• Computers and Concrete
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• v.13 no.2
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• pp.255-270
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• 2014

Impact performance of high-performance concrete (HPC) and SFRC at 28-day and 56-day under the action of repeated dynamic loading was studied. Silica fume replacement at 10% and 15% by mass and crimped steel fiber ($V_f$ = 0.5%- 1.5%) with aspect ratios of 80 and 53 were used in the concrete mixes. Results indicated that addition of fibers in HPC can effectively restrain the initiation and propagation of cracks under stress, and enhance the impact strengths and toughness of HPC. Variation of fiber aspect ratio has minor effect on improvement in impact strength. Based on the experimental data, failure resistance prediction models were developed with correlation coefficient (R) = 0.96 and the estimated absolute variation is 1.82% and on validation, the integral absolute error (IAE) determined is 10.49%. On analyzing the data collected, linear relationship for the prediction of failure resistance with R= 0.99 was obtained. IAE value of 10.26% for the model indicates better the reliability of model. Multiple linear regression model was developed to predict the ultimate failure resistance with multiple R= 0.96 and absolute variation obtained is 4.9%.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.8
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• pp.1066-1074
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• 2015

In contrast to high genetic diversity of mitochondrial DNA (mtDNA), equine Y chromosome shows extremely low variability, implying limited patrilines in the domesticated horse. In this study, we applied direct sequencing and restriction fragment length polymorphism (RFLP) methods to investigate the polymorphisms of 33 Y chromosome specific loci in 304 Chinese indigenous horses from 13 breeds. Consequently, two Y-single nucleotide polymorphisms (SNPs) (Y-45701/997 and Y-50869) and one Y-indel (Y-45288) were identified. Of those, the Y-50869 (T>A) revealed the highest variation frequency (24.67%), whereas it was only 3.29% and 1.97% in Y-45288 (T/-) and Y-45701/997 (G>T) locus, respectively. These three mutations accounted for 27.96% of the total samples and identified five Y-SNP haplotypes, demonstrating genetic diversity of Y chromosome in Chinese horses. In addition, all the five YSNP haplotypes were shared by different breeds. Among 13 horse breeds analyzed, Balikun horse displayed the highest nucleotide diversity (${\pi}=5.6{\times}10^{-4}$) and haplotype diversity (h = 0.527), while Ningqiang horse showed the lowest nucleotide diversity (${\pi}=0.00000$) and haplotype diversity (h = 0.000). The results also revealed that Chinese horses had a different polymorphic pattern of Y chromosome from European and American horses. In conclusion, Chinese horses revealed genetic diversity of Y chromosome, however more efforts should be made to better understand the domestication and paternal origin of Chinese indigenous horses.

• Journal of the Korean Mathematical Society
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• v.44 no.4
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• pp.1025-1050
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• 2007

Let $X_k(x)=({\int}^T_o{\alpha}_1(s)dx(s),...,{\int}^T_o{\alpha}_k(s)dx(s))\;and\;X_{\tau}(x)=(x(t_1),...,x(t_k))$ on the classical Wiener space, where ${{\alpha}_1,...,{\alpha}_k}$ is an orthonormal subset of $L_2$ [0, T] and ${\tau}:0 is a partition of [0, T]. In this paper, we establish a change of scale formula for conditional Wiener integrals $E[G_{\gamma}|X_k]$ of functions on classical Wiener space having the form $$G_{\gamma}(x)=F(x){\Psi}({\int}^T_ov_1(s)dx(s),...,{\int}^T_o\;v_{\gamma}(s)dx(s))$$, for $F{\in}S\;and\;{\Psi}={\psi}+{\phi}({\psi}{\in}L_p(\mathbb{R}^{\gamma}),\;{\phi}{\in}\hat{M}(\mathbb{R}^{\gamma}))$, which need not be bounded or continuous. Here S is a Banach algebra on classical Wiener space and $\hat{M}(\mathbb{R}^{\gamma})$ is the space of Fourier transforms of measures of bounded variation over $\mathbb{R}^{\gamma}$. As results of the formula, we derive a change of scale formula for the conditional Wiener integrals $E[G_{\gamma}|X_{\tau}]\;and\;E[F|X_{\tau}]$. Finally, we show that the analytic Feynman integral of F can be expressed as a limit of a change of scale transformation of the conditional Wiener integral of F using an inversion formula which changes the conditional Wiener integral of F to an ordinary Wiener integral of F, and then we obtain another type of change of scale formula for Wiener integrals of F.

• Journal of Species Research
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• v.1 no.2
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• pp.224-231
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• 2012

To assess the genetic diversity of Aconitum coreanum (Ranunculaceae) populations in Korea, we have amplified and sequenced eight organellar marker regions, and developed and analyzed microsatellite markers. No sequence variation was detected from the eight organellar markers. Ten microsatellites were developed using Next Generation Sequencing and two microsatellite markers, AK_CA03 and AK_CT07, were identified polymorphic and applied for 143 individuals of twelve A. coreanum populations. Four and five alleles were detected for the two microsatellite loci, respectively, and number of migrants ($N_m$) was estimated as 1.12586. Two microsatellite marker loci showed $F_{ST}$ of 0.205 and 0.275, respectively. The heterozygosity deficit, low level of among-population differentiation, small size of gene flow, and lack of sequence variation of the organellar markers suggest that A. coreanum is reproductively isolated from other Aconitum species and there has been continuous gene flow among the populations of A. coreanum or it has dispersed relatively recently after speciation. Though population pairwise $F_{ST}$'s presented significant geographic structure, further sampling and study will be necessary to confirm this.