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Isolation and Characterization of Three Kinds of Lipopeptides Produced by Bacillus subtilis JKK238 from Jeot-Kal of Korean Traditional Fermented Fishes (한국 전통젓갈에서 분리한 Bacillus subtilis JKK238 균주 유래 세 종류 Lipopeptide의 분리 및 특성)

  • Yoon Sang-Hong;Kim Jung-Bong;Lim Yoong-Ho;Hong Seong-Ryeul;Song Jae-Kyeung;Kim Sam-Sun;Kwon Soon-Wo;Park In-Cheol;Kim Soo-Jin;Yeo Yun-Soo;Koo Bon-Sung
    • Microbiology and Biotechnology Letters
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    • v.33 no.4
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    • pp.295-301
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    • 2005
  • About seven hundred bacterial strains were collected from Jeot-Kal, a Korean traditional fermented fishes, in various Korean districts. One of the strains designated JKK238 has its ability to antagonize in vitro the growth of a wide variety of plant pathogenic fungi responsible for diseases of economical importance. The JKK238 strain was isolated from Oh-Jeot, a kind of fermented shrimps, of Kangkyeung in Korea, and was identified as Bacillus subtilis based on its physiological characteristics, fatty acids compositions of cellular wall, and 16S rDNA sequence analysis. We isolated simply antimicrobial lipopeptides (AMLP) by $25\%$ ammonium sulfate precipitation of 3 days-old tryptic soy broth cultures of the JKK238 strain. Further analysis of AMLP revealed that B. subtilis JKK238 produces a wide variety of antifungal lipopeptide isomers from the iturin, fengycin and surfactin families simultaneously. Above results indicate that the JKK238 strain can be added to the limited number B. subtilis strains reported to co-produce the three kinds of lipopeptide families.

The Use of Polypropylene Mesh for Perineal Herniorrhaphy in the Dog (개에서 polypropylene mesh를 이용한 회음부 탈장 교정술 증례)

  • Kang, Eun-Hee;Chang, Hwa-Seok;Yang, Hee-Taek;Chung, Dai-Jung;Lee, Jae-Hoon;Yang, Wo-Jong;Kim, Tae-Hoon;Lee, Young-Su;Choi, Chi-Bong;Kim, Hwi-Yool
    • Journal of Veterinary Clinics
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    • v.23 no.4
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    • pp.461-464
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    • 2006
  • Perineal hernia occurs spontaneously in older male dogs after idiopathic weakening of the pelvic diaphragm A 14-year-old uncastrated male shih tzu dog with right-sided perineal swelling was referred to the Veterinary Medical Teaching Hospital of Konkuk University. He had sign of perineal swelling for three years. Plain radiography documented the extent of rectal and colonic dilation in the herinal sac. During surgery, external anal sphincter muscle, coccygeus muscle and levator am muscle were weakened due to the three years of herniation. Internal obturator muscle transposition method was not enough for large defect, so mesh was applied to reduce the hernial sac. Internal obturator muscle transposition combined with using of polypropylene mesh was successfully performed in this dog.

Ventral Fixation of Atlantoaxial Joint under Fluoroscopic Guidance Using Screws in a Chihuahua Dog (환축추 아탈구 견에서 투시기 유도를 통한 복측 나사 고정술)

  • Lee, Jae-Hoon;Yang, Hee-Taek;Yang, Wo-Jong;Chung, Dai-Jung;Kang, Eun-Hee;Eom, Ki-Dong;Choi, Chi-Bong;Chang, Hwa-Seok;Kim, Hwi-Yool
    • Journal of Veterinary Clinics
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    • v.25 no.2
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    • pp.131-135
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    • 2008
  • A 5-year-old Chihuahua dog was evaluated for a tetraparesis. The dog was presented with a non-ambulatory tetraparesis and neck pain. Radiography demonstrated an atlantoaxial (AA) subluxation and increased distance between the dorsal arch of the C1 and the dorsal spinous process of the C2. The AA joint was fixed with ventral transarticular fixation using two screws under fluoroscopic guidance. Neck brace was applied during 3 weeks post-operation. After 4 weeks, the dog was fully ambulatory with improved neurological function. Postoperative radiographs confirmed reduction of the luxation and no migration of screws. The dog showed complete resolution of clinical signs without signs of recurrence till 4 months after operation. Proper angulations of screws provided under fluoroscopic guidance, enabled adequate stabilization of the AA joints using ventral transarticular screw fixation which improved neurologic outcome of the patient.

Diversity and Phylogenetic Analysis of Fluorescent Pseudomonads Isolated from Soil-Root System of Red Pepper in Greenhouse (비닐하우스 고추재배지의 토양과 근계로부터 분리된 형광성 Pseudomonads의 계통 분류 및 다양성)

  • Kwon, Soon-Wo;Kim, Jong-Shik;Song, Jae-Kyeong;Ryu, Jin-Chang
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.4
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    • pp.275-282
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    • 2000
  • Among the fluorescent pseudomonad isolates from soil- root system of red pepper in Chinju, Kyunsangnam-Do, the phylogenetic analysis for 35 isolates were conducted. The partial 16S ribosomal DNA sequences were used as taxonomic key for phylogenetic analyses, and these sequences were enabled to identification of the fluorescent pseudomonad isolates on the species level. The 17 isolates among them were classified into Pseudomonas putida group, and consisted of the strains isolated mainly from soil. This group were subdivided into 4 subgroups (I, II, III, and IV). The subgroup I and IV were unique ones which were relatively remotely related with subgroup II and III including the type strain of P. putida. The 15 isolates among 35 isolates were grouped along with the type strain of Pseudomonas fluorescens, and 3 isolate were characterized as intermediates of P. fluorescens and Pseudomonas chlororaphis. Most of strain isolateds from the rhizosphere soil and rhizoplane of red pepper were identified as P. fluorescens and closely related with each other. In this study, root of red pepper was supposed to be colonized by a specific strain or strains of P. fluorescens.

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Analysis of Hydraulic Characteristics of Flood Plain Using Two-Dimensional Unsteady Model (2차원 부정류 모형을 이용한 둔치의 수리특성 분석)

  • Ku, Young Hun;Song, Chang Geun;Kim, Young Do;Seo, Il Wo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.33 no.3
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    • pp.997-1005
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    • 2013
  • Since the cross-sectional shape of the Nakdong river is compound type, the water stage rises up to the top of the flood plane, as the flow discharge increases during the extreme rain storm in summer. The recent increase of rainfall intensity and flood frequency results in the immersions of parks and hydrophilic facilities located in the flood plain. Therefore it is necessary to analyze the hydraulic characteristics evolved by the extreme rain storm in the flood plain. The study reach ranging from the Gangjeong Goryeong Weir and the Dalseong Weir, where several hydraulic facilities are located along the channel, was selected and numerical simulations were conducted for 42 hours including the peak flood of the typhoon Sanba. The 2-D transient model, FaSTMECH was employed and the accuracy of the model was assessed by comparing the water level between the simulation results and the measured ones at a gauging station. It showed a high correlation with $R^2$ of 0.990, AME of 0.195, and RMSE of 0.252. In addition, the inundation time, the inundation depth, the inundation velocity, and the shear stress variation in the flood plain facilities were analyzed.

The Mycobiota of Air Inside and Outside the Meju Fermentation Room and the Origin of Meju Fungi

  • Kim, Dae-Ho;Kim, Sun-Hwa;Kwon, Soon-wo;Lee, Jong-Kyu;Hong, Seung-Beom
    • Mycobiology
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    • v.43 no.3
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    • pp.258-265
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    • 2015
  • The fungi on Meju are known to play an important role as degrader of macromolecule of soybeans. In order to elucidate the origin of fungi on traditional Meju, mycobiota of the air both inside and outside traditional Meju fermentation rooms was examined. From 11 samples of air collected from inside and outside of 7 Meju fermentation rooms, 37 genera and 90 species of fungi were identified. In outside air of the fermentation room, Cladosporium sp. and Cladosporium cladosporioides were the dominant species, followed by Cladosporium tenuissimum, Eurotium sp., Phoma sp., Sistotrema brinkmannii, Alternaria sp., Aspergillus fumigatus, Schizophyllum commune, and Penicillium glabrum. In inside air of the fermentation room, Cladosporium sp., Aspergillus oryzae, Penicillium chrysogenum, Asp. nidulans, Aspergillus sp., Cla. cladosporioides, Eurotium sp., Penicillium sp., Cla. tenuissimum, Asp. niger, Eur. herbariorum, Asp. sydowii, and Eur. repens were collected with high frequency. The concentrations of the genera Aspergillus, Eurotium, and Penicillium were significantly higher in inside air than outside air. From this result and those of previous reports, the origin of fungi present on Meju was inferred. Of the dominant fungal species present on Meju, Lichtheimia ramosa, Mucor circinelloides, Mucor racemosus, and Scopulariopsis brevicaulis are thought to be originated from outside air, because these species are not or are rarely isolated from rice straw and soybean; however, they were detected outside air of fermentation room and are species commonly found in indoor environments. However, Asp. oryzae, Pen. polonicum, Eur. repens, Pen. solitum, and Eur. chevalieri, which are frequently found on Meju, are common in rice straw and could be transferred from rice straw to Meju. The fungi grow and produce abundant spores during Meju fermentation, and after the spores accumulate in the air of fermentation room, they could influence mycobiota of Meju fermentation in the following year. This could explain why concentrations of the genera Aspergillus, Eurotium, and Penicillium are much higher inside than outside of the fermentation rooms.

Research Performance Evaluation Based on Quantitative Information Analysis in the Field of Herbal Medicine for Dementia Treatment (계량정보분석 기반의 연구개발 성과분석 : 치매 치료용 천연약물 분야)

  • Jeon, Won-Kyung;Han, Chang-Hyun;Kang, Jong-Seok;Heo, Eun-Jung;Han, Joong-Su;Lee, Young-Joon
    • Journal of Oriental Neuropsychiatry
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    • v.22 no.3
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    • pp.101-113
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    • 2011
  • Objectives : Trend of R&D of herbal medicine for dementia treatment was examined based on the quantitative information analysis for establishing the national strategy of research on dementia treatment with oriental medicine. Methods : Definition was made to clarify the technology for development of herbal medicine for dementia treatment. Based on the initial keyword provided by experts in the field, queries were compounded to conduct search in the search engines of WoS and DWPI. The raw data (papers or patents) extracted from the initial search were examined by expert-review before objects of analysis were determined. Then, the accumulated data was analyzed in terms of year, country and organization, which led to examination of the trend of R&D. And the research performance evaluation for dementia treatment technologies was also made in terms of country, organization and researcher based on the forward citation analysis. The international cooperation intensity was examined on the basis of analysis of network by researcher before analysis results were put together to select lead researchers. Results : According to the quantitative information analysis of 1,330 articles that were selected as analysis objects, the number of papers on natural products research for dementia treatment has increased by around 4.6 times in recent five years. This indicates that the intensive studies have been underway recently. It was found to be the US that had the highest level in research filed of herbal medicine for dementia treatment and the highest capacity of international cooperation for that purpose. On the contrary, Korea had the share of papers at 5.1%, the number of countries in cooperation research at 8, and the article quality index at 0.40, showing that the qualitative level was insufficient, compared to the quantitative outcome. In particular, Korea was found to have no intensity of international cooperation among researchers. In case of patent, the results of information analysis of 305 patents selected as analysis objects demonstrated that China had the highest share while Korea had the very low frequency of patent application quantitatively. Conclusions : In this study, the research to develop herbal medicine for dementia treatment has recently drawn much attention that has spread around the globe. Therefore, these results suggest establishing the strategy to develop technology for dementia treatment with oriental medicine in the future based on quantitative information analysis.

Synthesis and Anti-corrosion Properties of Succinic Acid Alkyl Half-Ester Derivatives (숙신산 알킬 하프-에스테르 유도체의 합성 및 해수에 대한 방청성능)

  • Baek, Seung-Yeob;Kim, Young-Wun;Chung, Keun-Wo;Yoo, Seung-Hyun
    • Applied Chemistry for Engineering
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    • v.22 no.4
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    • pp.367-375
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    • 2011
  • Succinic acid ester derivatives have been used as additives for the base oil of metal working fluids and pressure working oils. In this paper, a series of succinic acid alkyl half-esters were synthesized with over 97% yields by ring-opening reaction of succinic anhydride and fatty alcohol and were soluble in 100 N base oil within 1 wt% concentration. The structures and purities of ester derivatives were confirmed by $^1H-NMR$ and FT-IR spectrum and GC analysis. Anti-corrosion properties of the esters in sea water were evaluated through ASTM D665 method and weight loss method and compared to that of succinic alkyl esters without carboxylic acid group in the molecule. As the results, anti-corrosion properties of succinic acid alkyl half-esters with carboxylic acid group were better than those of succinic acid alkyl esters without carboxylic acid group. And, Anti-corrosion properties of the esters with a shorter alkyl chain of high concentration showed better performance than those with longer alkyl chain of low concentration. Inhibition efficiency % (IE%) of the esters was over 95% in the concentration of 80 ppm and corrosion rate (CR) was below 0.3 mm/year at the same concentration. Thus, the corrosion properties of succinic acid alkyl half-esters result from the carboxylic acid groups in molecules.

Antioxidant Activities of Various Black Soybean Tissues (Glycine max L.) Harvested from Different Cultivation Regions (재배지역에 따른 검정콩 부위별 추출물의 항산화 활성)

  • Kim, Hyun Young;Wo, So-Yeun;Yang, Ji Yeong;Song, Seung-Yeob;Seo, Woo Duck;Lee, Mi Ja;Choi, Man-Soo
    • The Korean Journal of Food And Nutrition
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    • v.34 no.4
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    • pp.331-339
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    • 2021
  • With the aim of developing region specialized crops, this study was conducted to clarify effects of variant and cultivation region on antioxidative activities in various black soybean (Glycine max L.) seed tissues. Three black soybean varieties (SCEL-1, Wonheug, and Cheongja 3) were each cultivated in 4 different regions (Jeonju, Pyeongchang, Paju, and Cheonan). Harvested seeds were used to assess DPPH and ABTS radical scavenging activity, and total polyphenol, flavonoid and anthocyanin content. SCEL-1 soybean hull contained higher DPPH and ABTS radical scavenging activity (61% and 85% respectively) compared to Wonheug (40% and 50% respectively). SCEL-1 cultivated in Pyeongchang displayed the highest total polyphenol and flavonoid content (1,189 mg GAE/100g sample and 951 mg CTE/ 100g sample, respectively). Total anthocyanin content was ranked in the following order: SCEL-1>Wonheug>Cheongja 3. All black soybeans showed much higher antioxidant activity in the soybean hull than in the dehulled soybean. The antioxidant activity of black soybeans cultivated at high latitudes was high. These results suggest that the best black soybean variant for high beneficial biological activities is the SCEL-1 variant. For a complete understanding of the potential of black soybean as functional foods, we plan to further analyze their antioxidant activities in future studies.

Topic Model Augmentation and Extension Method using LDA and BERTopic (LDA와 BERTopic을 이용한 토픽모델링의 증강과 확장 기법 연구)

  • Kim, SeonWook;Yang, Kiduk
    • Journal of the Korean Society for information Management
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    • v.39 no.3
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    • pp.99-132
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    • 2022
  • The purpose of this study is to propose AET (Augmented and Extended Topics), a novel method of synthesizing both LDA and BERTopic results, and to analyze the recently published LIS articles as an experimental approach. To achieve the purpose of this study, 55,442 abstracts from 85 LIS journals within the WoS database, which spans from January 2001 to October 2021, were analyzed. AET first constructs a WORD2VEC-based cosine similarity matrix between LDA and BERTopic results, extracts AT (Augmented Topics) by repeating the matrix reordering and segmentation procedures as long as their semantic relations are still valid, and finally determines ET (Extended Topics) by removing any LDA related residual subtopics from the matrix and ordering the rest of them by F1 (BERTopic topic size rank, Inverse cosine similarity rank). AET, by comparing with the baseline LDA result, shows that AT has effectively concretized the original LDA topic model and ET has discovered new meaningful topics that LDA didn't. When it comes to the qualitative performance evaluation, AT performs better than LDA while ET shows similar performances except in a few cases.