• Asian-Australasian Journal of Animal Sciences
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• v.16 no.6
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• pp.818-822
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• 2003

In order to investigate the effect of different sources of energy on intake and nutrient utilization from urea molasses mineral block (UMMB), rumen fermentation pattern and blood biochemical constituents, 18 intact and 9 rumen fistulated male Murrah buffaloes aged about 3 years and average weight 310.8 kg were randomly allocated into three groups of 9 animals in each, thus each group having 6 intact and three rumen fistulated buffaloes. All animals were fed individually for 90 days. All buffaloes were offered wheat straw as basal roughage and urea molasses mineral block for free choice of licking. Three different energy sources viz., barley grain, (group I), maize grain (group II) and jowar green (group III) were offered to meet their nutrient requirement as per Kearl (1982). At the end of feeding trial, a metabolism trial of 7 days duration was carried out on intact animals to determine the digestibility of nutrients. Rumen fermentation studies were carried out on rumen fistulated animals. After the metabolism trial blood was collected from intact animals to estimate the nitrogen constituents in blood serum of animals fed on different sources of energy. Results revealed no significant difference in the intake of UMMB in three groups. Similarly, the intake of DM (kg), DCP (g) and TDN (kg) per day was similar in three groups statistically. The apparent digestibility of dry matter (DM), organic matter (OM), ether extract (EE) and nitrogen free extract (NFE) was significantly (p<0.05) more in group II than group III, whereas the digestibility of DM, OM and NFE was similar in group I and II. The digestibility of crude fiber (CF) and all the fiber fractions i.e. NDF, ADF, cellulose and hemicellulose was alike in 3 groups. Nitrogen balance (g/d) was significantly (p<0.05) more in group III as compared to group I and II, which were alike statistically, though the N intake (g/d) was similar in 3 groups but N balance (g/d) was significantly (p<0.05) less in group III as compared to other 2 groups. Significantly (p<0.05) higher concentration of total volatile fatty acids (TVFA), total nitrogen (TN) and its fractions were observed in group I and II as compared to group III. There was no effect on rumen pH, rumen volume and digesta flow rate due to different sources of energy in 3 groups. Similarly the blood serum biochemical parameters (NH3-N, urea-N and total protein) were statistically identical in 3 groups.

• International Journal of Stem Cells
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• v.16 no.4
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• pp.425-437
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• 2023

Obesity, which continues to increase worldwide, was shown to irreversibly impair the differentiation potential and angiogenic properties of adipose tissue mesenchymal stromal cells (ADSCs). Because these cells are intended for regenerative medicine, especially for the treatment of inflammatory conditions, and the effects of obesity on the immunomodulatory properties of ADSCs are not yet clear, here we investigated how ADSCs isolated from former obese subjects (Ex-Ob) would influence macrophage differentiation and polarization, since these cells are the main instructors of inflammatory responses. Analysis of the subcutaneous adipose tissue (SAT) of overweight (OW) and Ex-Ob subjects showed the maintenance of approximately twice as many macrophages in Ex-Ob SAT, contained within the CD68⁺/FXIII-A^- inflammatory pool. Despite it, in vitro, coculture experiments revealed that Ex-Ob ADSCs instructed monocyte differentiation into a M2-like profile, and under inflammatory conditions induced by LPS treatment, inhibited HLA-DR upregulation by resting M0 macrophages, originated a similar percentage of TNF-α⁺ cells, and inhibited IL-10 secretion, similar to OW-ADSCs and BMSCs, which were used for comparison, as these are the main alternative cell types available for therapeutic purposes. Our results showed that Ex-Ob ADSCs mirrored OW-ADSCs in macrophage education, favoring the M2 immunophenotype and a mixed (M1/M2) secretory response. These results have translational potential, since they provide evidence that ADSCs from both Ex-Ob and OW subjects can be used in regenerative medicine in eligible therapies. Further in vivo studies will be fundamental to validate these observations.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 1995.10a
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• pp.160-164
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• 1995

정보통신 시스템의 고속/고밀도화 요구에 따라 개발되고 있는 ATM(Asynchronous Transfer Mode) 교환기 시스템은 팬을 이용한 강제대류냉각 방식의 채택과 시스템이 설치되는 장소에 따른 여러 환경조건에 의한 진동 문제가 발생될 수 있다. 시스템의 진동으로 인한 피해중 커넥터 접촉부에서 전기적 특성의 변화는 고속으로 전송되는 신호의 왜곡을 유발시킬 수 있어 시스템 개발시 이에 대한 충분한 연구 및 시험이 요구되고 있다. 진동환경에서 커넥터 접촉부는 접촉면의 상대운동으로 인한 접촉저항의 증가와 순간적인 신호전달 중단을 가져오게 되며, 특히 PCB/Connector Assembly에서 커넥터 접촉부는 PCB(Printed Circuit Board)의 장착 조건 및 동적 거동에 따라 전기적 특성이 변할 수 있다. 시스템에서 커넥터의 동적 거동을 이해하기 위해서는 PCB를 포함하는 시스템내 여러 요소의 동적 특성 이해와 복잡한 해석과정이 요구되며, 시스템 개발자는 진동 환경에서 이것의 시험 결과에 따라 커넥터의 사용을 결정해야 할 것이다. 커넥터의 전기적 특성 시험법은 IEC, EIA드 여러 국제 규격에 제시되어 있으며, 본 연구의 대상이 된 ATM교환기 시스템에서 PCB/Connector Assembly의 진동환경에서 접촉저항 측정과 관련된 접촉저항 임계치 및 측정법은 IEEE 규격 및 Bellcore 규격에 규정되어 있다. Bellcore에는 주어진 진동시험주기 전후에 IEC 규격의 LLCR(Low Level Contact Resistance) 측정회로를 이용한 측정법이 규정되어 있고, 냉각팬 및 주위 환경진동이 가해지는 동안의 영향에 대한 시험법은 규정되어 있지 않다. 본 연구에서는 한국통신의 전자장비 운용환경시험 조건의 진동에서 ATM 교환기 시스템에 사용되는 PCB/Connector Assembly 커넥터 접촉부의 접촉저항 변화와 PCB 진동에 의한 영향을 시험하였다.proach)등이 제시되었고 평면파 영역에 한하여 해서되어져 왔다. 본 논문에서는 분할 접근 방법(Segmentation Approach)을 이용하여 다공 요소로 이루어진 소음기를 해석하는데 적용하였다.로 성능 및 안정도에 영향을 미치므로 주의 깊게 선정해야 한다. 방법의 실질적인 적용에는 어려움이 있다. 본 연구에서는 기존의 방법들의 단점을 극복할 수 있는 새로운 회귀적 모우드 변수 규명 방법을 개발하였다. 이는 Fassois와 Lee가 ARMAX모델의 계수를 효율적으로 추정하기 위하여 개발한 뱉치방법인 Suboptimum Maximum Likelihood 방법[5]를 기초로 하여 개발하였다. 개발된 방법의 장점은 응답 신호에 유색잡음이 존재하여도 모우드 변수들을 항상 정확하게 구할 수 있으며, 또한 알고리즘의 안정성이 보장된 것이다.. 여기서는 실험실 수준의 평 판모델을 제작하고 실제 현장에서 이루어질 수 있는 진동제어 구조물에 대 한 동적실험 및 FRS를 수행하는 과정과 동일하게 따름으로써 실제 발생할 수 있는 오차나 error를 실험실내의 차원에서 파악하여 진동원을 있는 구조 물에 대한 진동제어기술을 보유하고자 한다. 이용한 해마의 부피측정은 해마경화증 환자의 진단에 있어 육안적인 MR 진단이 어려운 제한된 경우에만 실제적 도움을 줄 수 있는 보조적인 방법으로 생각된다.ofile whereas relaxivity at high field is not affected by τS. On the other hand, the change in τV does not affect low field profile but strongly in fluences on both inflection fie이 and the maximum relaxivity value. The results shows a fluences on both inflection field and the maximum relaxivity v

• Journal of Life Science
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• v.31 no.10
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• pp.922-928
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• 2021

To profile the microbial compositions of Korean traditional fermented paste made from whole soybeans, Doenjang and Cheonggukjang, and compare their taxonomic differences, we analyzed the V3-V4 region of 16S rRNA of naturally fermented foods by using next generation sequencing. α-Diversity results showed that values indicating bacterial community abundances (OTUs) and richness (ACE, Chao1) were statistically significant (p=0.0001) in Doenjang and Cheonggukjang. Firmicutes was the most common phylum in both groups, representing 97.02% and 99.67% in the Doenjang and Cheonggukjang groups, respectively. Bacillus was the most dominant genus, accounting for 71.70% and 59.87% in both groups. Linear discriminant (LDA) effect size (LEfSe) analysis was performed to reveal the significant ranking of abundant taxa in different fermented foods. A size-effect threshold of 2.0 on the logarithmic LDA score was used for discriminative functional biomarkers. On the species level, Bacillus subtilis, Tetragenococcus halophilus, and Clostridium arbusti were significantly more abundant in Doenjang than in Cheonggukjang, whereas Bacillus thermoamylovorans, Enterococcus faecium, and Lactobacillus sakei were significantly more abundant in Cheonggukjang than in Doenjang. Permutational multivariate analysis of variance (PERMANOVA) showed that the statistical difference in microbial clusters between the two groups was significant at the confidence level of p=0.001. This research could be used as basic research to identify the correlation between the biochemical characteristics of Korean fermented foods and the distribution of microbial communities.

• Ocean and Polar Research
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• v.24 no.4
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• pp.483-490
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• 2002

Detailed investigations on both submarine and subaerial volcaniclastic deposits around Dokdo were carried out to identify geomorphologic characteristics, stratigraphy, and associated depositional processes of Dokdo caldera. Dokdo volcano has a gently sloping summit (about 11km in diameter) and relatively steep slope (basal diameter is about 20-25 km) rising above sea level at about 2,270m. We found ragged, elliptical-form of Dokdo caldera with a diameter of about 2km estimated by Chirp (3-11 kHz) sub-bottom profile data and side scan sonar data for the central summit area of Dokdo volcano. We interpreted that the volcaniclastic deposits of Dokdo unconformably consist of the Seodo (west islet) and the Dongdo(east islet) formations based on internal structure, constituent mineral composition, and bedding morphology. The Seodo Formation mainly consisted of massive or inversely graded trachytic breccias (Unit S-I), overlain by fine-grained tuff (Unit S-II), which is probably supplied by mass-wasting processes resulting from Dokdo caldera collapse. The Dongdo Formation consists of alternated units of stratified lapilli tuff and inversely graded basaltic breccia (Unit D-I, Unit D-III, and Unit D-V), and massive to undulatory-bedded basaltic tuff breccias (Unit D-II and Unit D-IV) formed by a repetitive pyroclastic surge and reworking processes. Although, two islets of Dokdo are geographically near each other, they have different formations reflecting their different depositional processes and eruptive stages.

• Bulletin of the Korean Chemical Society
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• v.35 no.8
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• pp.2391-2399
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• 2014

Novel dual responsive pectin hydrogels composed from poly(acrylamidoglycolic acid-co-vinylcaprolactam)/Pectin (PAV-PC) and also PAV-PC hydrogels are used as templates for the production of silver nanoparticles. 5-Fluorouracil is an anticancer drug and has been loaded in situ into PAV-PC hydrogels. Structure and morphology characterization of PAV-PC hydrogels were investigated by fourier transform infrared spectroscopy, differential scanning calorimetry, thermo gravimetric analysis, X-ray diffraction studies, scanning electron microscopy and transmission electron microscopy. The results revealed a molecular level dispersion of the drug in PAV-PC hydrogels. In vitro release of 5-fluorouracil from the PAV-PC hydrogels has been carried out in GIT fluids as well as in various temperatures. 5-Fluorouracil released from PAV-PC hydrogels was 50% at pH 1.2, and 85% at pH 7.4 within 24 h. The release profile was characterized with PAV-PC hydrogels and initial burst effect was significantly reduced in two buffer media (1.2 and 7.4), followed by a continuous and controlled release phase, the drug release mechanism from polymer was due to Fickian diffusion. In situ fabrication of silver nanoparticles inside the hydrogel network via the reduction of sodium borohydrate by PAV-PC chains led to hydrogel nanocomposites. The diameter of the nanocomposites was about 50-100 nm, suitable for uptake within the gastrointestinal tract due to their nanosize range and mucoadhesive properties. These nanocomposite PAV-PC hydrogels showed strong antimicrobial activity towards Bacillus subtilis (G+ve) and Escherichia coli (G-ve).

• Parasites, Hosts and Diseases
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• v.61 no.1
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• pp.42-52
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• 2023

The genus Babesia includes parasites that can induce human and animal babesiosis, which are common in tropical and subtropical regions of the world. The gut microbiota has not been examined in hamsters infected by Babesia duncani. Red blood cells infected with B. duncani were injected into hamsters through intraperitoneal route. To evaluate the changes in gut microbiota, DNAs were extracted from small intestinal contents, acquired from hamsters during disease development. Then, the V4 region of the 16S rRNA gene of bacteria was sequenced using the Illumina sequencing platform. Gut microbiota alternation and composition were assessed according to the sequencing data, which were clustered with >97.0% sequence similarity to create amplicon sequence variants (ASVs). Bacteroidetes and Firmicutes were made up of the major components of the gut microbiota in all samples. The abundance of Bacteroidetes elevated after B. duncani infection than the B. duncani-free group, while Firmicutes and Desulfobacterota declined. Alpha diversity analysis demonstrated that the shown ASVs were substantially decreased in the highest parasitemia group than B. duncani-free and lower parasitemia groups. Potential biomarkers were discovered by Linear discriminant analysis Effect Size (LEfSe) analysis, which demonstrated that several bacterial families (including Muribaculaceae, Desulfovibrionaceae, Oscillospiraceae, Helicobacteraceae, Clostridia UGG014, Desulfovibrionaceae, and Lachnospiraceae) were potential biomarkers in B. duncani-infected hamsters. This research demonstrated that B. duncani infectious can modify the gut microbiota of hamsters.

• The Korean Journal of Pesticide Science
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• v.2 no.1
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• pp.46-52
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• 1998

The hydrolysis rate of insecticidal buprofezin(IUPAC : tert-butylimino-3-isopropyl-5-phenylperhydro-1,3,5-thiadiazin-4-one) in the range of pH 2.0 and 12.0 have been examined in 15%(v/v) aqueous dioxane at $45^{\circ}C$. The hydrolysis mechanism of buprofezin is proposed from the pH-effect, solvent effect(${\ell}{\gg}m$), thermodynamic parameter(${\Delta}H^{\neq}$=11.12 $Kcal{\cdot}mol^{-1}$ &, ${\Delta}S^{\neq}=5.0e.u.$), rate equation and hydrolysis product, l-isopropyl-3-phenyl urea. General acid catalyzed hydrolysis and specific acid catalyzed($k_{H3O+}$) hydrolysis through $A-S_{E}2$ and A-2(or $A_{AC}2$) reaction mechanism with orbital-control reaction proceed below pH 8.0 and above pH 9.0, the nucleophilic addition-elimination, $Ad_{N}-E$ mechanism via tetrahedral($sp^{3}$) intermediate is initiation by general base catalyzed($k_{H2O}$) reaction. Buprofezin was more stable in alkaline ($k=10^{-8}sec.^{-1}$) than acid solutions from the sigmoid pH-rate profile. And the half-life($t=\frac{1}{2}$) of hydrolysis reaction in neutral aqueous solution(pH 7.0) at $45^{\circ}C$ was about 3 months.

• The Journal of Engineering Geology
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• v.23 no.1
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• pp.47-55
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• 2013

To analyze lateral displacement of excavation walls exposed during the construction of Subway Line 1 in the Daegu region, inclinometer measurement data for sites D4, D5, and Y6 are investigated from the perspective of engineering geology. The study area, in the Banyawol Formation, Hayang Group, Gyeongsang Supergroup, is in the lower part of bedrock of andesitic volcanics, calcareous shale, sandstone, hornfels, and felsite dykes that are unconformably overlain by soil. The rock mass around the D4 site is classified as RMR-V grade and the maximum lateral displacement of 101.39 mm, toward N34W, was measured at a bedding-parallel fault, at a depth of 12 m. The rock mass around the D5 site is classified as RMR-IV grade and the maximum lateral displacement of 55.17 mm, toward the south, was measured at a lithologic contact between shale and felsite, at a depth of 14 m. The rock mass around the Y6 site is classified as RMR-III grade and the maximum lateral displacement of 12.65 mm, toward S52W, was measured at an unconformity between the soil and underlying bedrocks, at a depth of 7 m. The directions of lateral displacement in the excavation walls are vector sums of the directions perpendicular to the excavation wall and horizontally parallel to the excavation wall. Lateral displacement graphs according to depth in the soil profile show curvilinear trajectories, whereas those in bedrock show straight and rapid-displacement trajectories.

• Journal of the Earthquake Engineering Society of Korea
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• v.13 no.6
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• pp.11-25
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• 2009

A method of instrumentally estimating the seismic intensity (MMI) based on Fourier Acceleration Spectrum, which is the so-called 'FAS MMI method' of Sokolov and Wald (2002), was considered for its applicability to Korea. In order to implement the FAS MMI method, the empirical models of mean (m) and standard deviation (${\sigma}$) for Korea were derived for MMI ${\leq}$ IV according to individual seismic intensity by using the site-consistent horizontal FAS of 580 records from 65 isoseismal maps prepared based on the reported MMI of Korea Meteorological Administration. The site-consistent FAS at a site were obtained by correcting the observed FAS for the difference of the site amplification function relative to that of the target site of Class D station (Yun and Suh, 2007) which was evaluated to be a representative site for the generic soil profile of Korea. The FAS m model for MMI ${\leq}$ IV follows the overall linear relation in log space according to seismic intensities, featuring the FAS mean model for MMI = IV similar to that of the global model of Sokolov and Wald (2002). The ${\sigma}$-values of the FAS model are found to be greater than those of the global model for MMI ${\geq}$ V, while significantly lower than those of the global model for MMI = IV.