Various formulae for estimation of spring leaf production in mulberry trees were calculated and obtained. Four varieties of mulberry trees were used as the materials, and four characters, namely branch length (X$_1$), node number (X$_2$), branch diameter (X$_3$) and branch number per stock (X$_4$) were studied. The formulae to estimate the leaf yield of spring mulberry trees are as follows: 1. $Y_1$v$_1$= -26.8939+50.3950X$_1$+1.1403X$_2$ $Y_1$v$_2$= -372.1091+116.6371X$_1$+0.1984X$_2$ $Y_1$v$_3$= 149.8203+90.5125X$_1$-0.9775X$_2$ $Y_1$v$_4$= 108, 1496+59.4533X$_1$+1.4965X$_2$ Where $Y_1$v$_1$, $Y_1$v$_2$, $Y_1$v$_3$, $Y_1$v$_4$, are showed the estimated yield of the each variety, namely Gaeryang Seuban, Ilchirye, Nosang, and Suwon Sang No. 4, respectively. X$_1$ and X$_2$ denote the measured values of branch length and node number, respectively. 2. $Y_{7}$v$_1$= -54.4411+32.9869c1.1127X$_2$+21.7600X$_3$ $Y_{7}$v$_2$= -494.1480-1.8756X$_1$+0.9788X$_2$+110.0039X$_3$ $Y_{7}$v$_3$= 143.2836+29.1779X$_1$+0.1644X$_2$+48.4135X$_3$ $Y_{7}$v$_4$= 1243.2549+1.9454X$_1$+2.7118X$_2$-75.6669X$_3$ Where $Y_{7}$v$_1$, $Y_{7}$v$_2$, $Y_{7}$v$_3$, $Y_{7}$v$_4$, are the estimated yield of the each variety, namely Gaeryang-Seuban, Ilchirye, Nosang, Suwon Sang No 4, respectively. X$_1$, X$_2$, X$_3$ denote the measured values of each character, branch length, node number, branch diameter and branch number per stock, respectively. 3. $Y_{11}$v$_1$=233.4780+74.3713X$_1$+1.2912X$_2$+39.0420X$_3$-148.9300X$_4$ $Y_{11}$v$_2$=-317.0150+15.l524X$_1$+1.0861X$_2$+156.7973X$_3$-148.3742X$_4$ $Y_{11}$v$_3$=178.7011+29.8664X$_1$-0.2562X$_2$+102.4632X$_3$-83.2693X$_4$ $Y_{11}$v$_4$= 264.0062+47.7742X$_1$+2.6996X$_2$+92.8882X$_3$-192.3464X$_4$ Where $Y_{11}$v$_1$, $Y_{11}$v$_2$, $Y_{11}$v$_3$, $Y_{11}$v$_4$, are the estimated yield values of four varieties, and X$_1$, X$_2$, X$_3$, X$_4$, denote the measured values of four characters, namely branch length, node number, branch diameter and branch number per stock, respectively. The estimation method of mulberry spring leaf yield by measurement of some characters, in autumn the year before, could be the better method to determine the leaf yield of mulberry trees without destroying the leaves and without weighting the leaves of mulberry trees than the other methods.