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Determination of Hydroperoxyl/superoxide Anion Radical (HO2·/O2·-) Concentration in the Decomposition of Ozone Using a Kinetic Method

  • Kwon, Bum-Gun;Lee, Jai H.
    • Bulletin of the Korean Chemical Society
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    • v.27 no.11
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    • pp.1785-1790
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    • 2006
  • A novel kinetic method for determination of $HO_2{^{\cdot}}/O_2{^{{\cdot}-}}$ in ozone decomposition in water is described. In this study, potential interferences of $O_3$ and the hydroxyl radicals, $^{\cdot}OH_{(O3)}$, are suppressed by $HSO_3{^-}/SO_3{^{2-}}$. $HO_2{^{\cdot}}/O_2{^{{\cdot}-}}$ formed in ozone decomposition reduces $Fe^{3+}$-EDTA into $Fe^{2+}$-EDTA and subsequently the well-known Fenton-like (FL) reaction of $H_2O_2$ and $Fe^{2+}$-EDTA produces the hydroxyl radicals, $^{\cdot}OH_{(FL)}$. Benzoic acid (BA) scavenges $^{\cdot}OH_{(FL)}$ to produce OHBA, which are analyzed by fluorescence detection (${\lambda}_{ex}=320nm$ and ${\lambda}_{ex}=400nm$). The concentration of $HO_2{^{\cdot}}/O_2{^{{\cdot}-}}$ in ozone decomposition has been determined by the novel kinetic method using the experimentally determined half-life ($t_{1/2}$). The steady-state concentration of $HO_2{^{\cdot}}/O_2{^{{\cdot}-}}$ is proportional to the $O_3$ concentration at a given pH. However, the steady-state concentration of $HO_2{^{\cdot}}/O_2{^{{\cdot}-}}$ in ozone decomposition is inversely proportional to pH values. This pH dependence is due to significant loss of $O_2{^{{\cdot}-}}$ by $O_3$ at higher pH conditions. The steady-state concentrations of $HO_2{^{\cdot}}/O_2{^{{\cdot}-}}$ are in the range of $2.49({\pm}0.10){\times}10^{-9}M(pH=4.17){\sim}3.01({\pm}0.07){\times}10^{-10}M(pH=7.59)$ at $[O_3]_o=60{\mu}M$.

HAZ Crack Growth Behavior of Cr-Mo Steel at Elevated Temperature (Cr-Mo강 용접열영향부에서의 고온 균열성장거동 연구)

  • 윤기봉;신규인;정용근;이해무
    • Proceedings of the KWS Conference
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    • 1997.05a
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    • pp.65-68
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    • 1997
  • Fracture behavior of ex-serviced 1Cr-0.5Mo steel was measured at room(24$^{\circ}C$) and elevated(538$^{\circ}C$) temperature and compared with that measured with virgin 1Cr-0.5Mo steel. Compact C(T) specimens were machined from the base and welded test materials. In case of the C(T) specimens of the weld, fatigue precrack was introduced along the fusion line so that a crack growth should occur along the region of heat affected zone. It was observed that the J-R curve of the serviced material was significantly lower than that of the virgin material at room temperature. Brittle fracture was observed in the serviced material. On the other hand at elevated temperature no noticeable difference was found between the J-R curves of the virgin and the serviced material. The measured J-R curves were also compared with those of the 1.25Cr-0.5Mo steel from other literatures. Optical microscopy and SEM examination of the serviced material reveal the carbide in/along the grain boundary which shows material degradation due to long-term usage.

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Effect of ${Gd_2}{O_3}$ Addition on the Sintered Density and Resintered Density in <${UO_2}-{Gd_2}{O_3}$ Pellets (${UO_2}-{Gd_2}{O_3}$소결체에서 ${Gd_2}{O_3}$첨가가 소결 및 재소결밀도에 미치는 영향)

  • Baek, Jong-Hyeok
    • Korean Journal of Materials Research
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    • v.5 no.3
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    • pp.364-370
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    • 1995
  • ex-AUC U$O_{2}$ 분말과 $Gd_{2}$O_{3}$ 분말을 기계적으로 혼합하여 소결한 U$O_{2}$-$Gd_{2}$O_{3}$ 소결체의 밀도 변화와 재소결 후 밀도변화를 기공크기 및 분포의 변화로 서술하였다. 수소분위기에서 175$0^{\circ}C$, 4시간 동안 소결하였을 때, 순수 U$O_{2}$의 소결밀도는 97.2% T.D.였으나 6wt% $Gd_{2}$O_{3}$ 첨가까지는 U$O_{2}$-$Gd_{2}$O_{3}$의 소결밀도는 $U^{+4}$와 $Gd^{+4}$의 상호확산 때문에 약 90% T.D.로 급격히 감소하였다. 그러나 6wt% 이상의 $Gd_{2}$O_{3}$가 첨가되면 우라늄이온 산화아와 산소침입으로 인하여 소결밀도는 오히려 증가하였다. 1$700^{\circ}C$에서 재소결시킬 때 순수 U$O_{2}$ 소결체에서는 재소결 시간에 따라 밀도증가가 발생하였다. U$O_{2}$-$Gd_{2}$O_{3}$ 소결체 경우에는 재소결시 밀도가 감소하였으나 재소결 시간이 증가함에 따라 다시 밀도는 증가하였고, 6wt%$Gd_{2}$O_{3}$가 첨가된 U$O_{2}$-$Gd_{2}$O_{3}$ 소결체에서 밀도가 가장 많이 감소하였다.

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A Diode Bridge-type ZVT Inverter for Induction Motor Drive Applications (유도 전동기 구동용 다이오드 브릿지-타입 ZVT 인버터)

  • 이성룡;고성훈;송인석
    • The Transactions of the Korean Institute of Power Electronics
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    • v.4 no.6
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    • pp.561-569
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    • 1999
  • In this paper, the diode bridge-type ZVT(Zero- Voltage Transition) inverter is proposed. It consists of one a auxiliaI${\gamma}$ switch, three resonant inductors and six blockin당 diodes. So, the main advantage of the proposed t torXJ!ogy is the reduction of the auxiliar${\gamma}$ switch compare to the conventional ZVT inverter. The topology of t the propostxl lVT inverter is analyztxl with a description of the control conditions based on the load current. A And the resonant period control for operating the proposed inverter optimally is discussed by using resonant i inductor current ftxxlback. To verify the proposed topology, the detailed simulation and exJX끼mental results i indicate that zero volta당e operation during transition can be achieved.

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A Study on Elevated Temperature Fracture Behavior of Cracks in 1Cr-0.5Mo Steel Weld (1Cr-0.5Mo강 용접부 균열의 고온파괴거동 연구)

  • 신규인;윤기봉;최현창;박재학;이해무
    • Journal of the Korean Society of Safety
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    • v.12 no.2
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    • pp.45-56
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    • 1997
  • Fracture behavior of ex-serviced 1Cr-0.5Mo steel was measured at room($24^{\circ}C$) and elevated($538^{\circ}C$) temperature and compared with that measured with virgin 1Cr-0.5Mo steel. Compact C(T) specimens were machined from the base and welded test materials. In case of the C(T) specimens of the weld, fatigue precrack was introduced along the fusion line so that following crack growth should occurs along the region of heat affected zone. It was observed that the J-R curve of the serviced material was significantly lower than that of the virgin material at room temperature. Brittle fracture was observed in the serviced material. On the other hand, at elevated temperature no noticeable difference was found between the J-R curves of the virgin and the serviced material. The measured J-R curves were also compared with those of the 1.25Cr-0.5Mo steel obtained from the other literatures. Optical microscopy and SEM examination of the serviced material reveal the carbide in/along the grain boundary which shows material degradation due to long-term usage. An example of application of the measured J-R curves is shown.

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Enhancement of immune activities of Dioscorea japonica Thunberg in in vivo and ex vivo models

  • Lim, Seokwon;Kim, Na-Hyung;Cho, Hi-Jae;Jeong, Hyun-Ja
    • Korean Journal of Food Science and Technology
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    • v.51 no.4
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    • pp.398-403
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    • 2019
  • Dioscorea japonica Thunberg (DJ) has been widely used as a healthy food in Korea for the enhancement of physical stamina. Hence, the present study evaluated the immune-enhancing effect of DJ in forced swim test of mouse model. The immobility time of the group treated with DJ for 7 days was significantly reduced in comparison with that of the control group. After a forced swimming test, the changes in blood biochemical parameters and splenic T lymphocyte populations induced by the administration of DJ were assessed. Serum levels of lactic dehydrogenase, creatine phosphokinase, and aspartate aminotransferase were significantly decreased in DJ-administered group compared to the control group. However, administration of DJ did not affect the splenic T lymphocyte populations. Moreover, DJ significantly increased the production of interferon-g and interleukin-2 compared to the media control in splenocytes. Collectively, it may be concluded that DJ is useful for enhancement of physical and immune function.

Design and implementation of IoT platform for collecting and managing the SmartFactory environment information

  • Kim, SungJin;Ra, SangYong;Kim, HwanSeog;Choi, JaeHong;Lee, JunDong
    • Journal of the Korea Society of Computer and Information
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    • v.24 no.6
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    • pp.109-115
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    • 2019
  • Smart Factory is a part of and a key point of the 4th industrial revolution. It performs optimization from the whole viewpoint, using comprehensive data of the post-process data by utilizing various sensors, controllers, and mobile devices beyond the existing factory automation level. In this paper, we design and implement an IoT platform that can detect the safety factors of the workers, the environmental factors of the factory, and real time monitoring at the control center, among the fields to implement smart factory. To accomplish this, we construct a monitoring device that provides sensor information control, server transmission of sensor information, and visualization of collected information. By using this system, it is possible to maintain the temperature and humidity for the optimum working environment in the factory. and also, By using the beacon, it is possible to measure the working time of the worker and trace the position.

The Study of MHC class I Restricted CD8+ T Cell Mediated Immune Responses against Mycobacterium tuberculosis Infection: Evidence of M. tuberculosis S pecific CD8+ T Cells in TB Patients and PPD+ Healthy Individuals (MHC class I 분자들에 의해 제시되는 Epitope을 인지하는 CD8+ T 림프구의 결핵균 감염에 대한 면역반응의 연구: 결핵 환자와 PPD+ 건강개체에 존재하는 결핵균 항원에 특정한 CD8+ T세포)

  • Cho, Jang-Eun;Lee, Kyung Wha;Park, Seung Kyu;Cheon, Seon-Hee;Cho, Sang-Nae;Cho, Sungae
    • IMMUNE NETWORK
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    • v.3 no.3
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    • pp.235-241
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    • 2003
  • Background: The protective immunity against tuberculosis (TB) involves both CD4+ T cells and CD8+ T cells. In our previous study, we defined four Mycobacterium tuberculosis derived peptide epitopes specific for HLA-$A^*0201$ restricted CD8+ T cells ($ThyA_{30-38}$, $RpoB_{127-135}$, $85B_{15-23}$, $PstA1_{75-83}$). In this study, we investigated the immune responses induced by these peptide specific CD8+ T cells in latently and chronically infected people with TB. Methods: We characterized these peptide specific CD8+ T cell population present in PBMC of both TB patients and PPD+healthy people using IFN-${\gamma}$elispot assay, intracellular staining and HLA-A2 dimer staining. Results: The frequency of peptide specific CD8+ T cell was in the range of 1 to 25 in $1.7{\times}10^5$ PBMC based on ex vivo IFN-${\gamma}$ elispot assay, demonstrating that these peptide specific CD8+ T cell responses are induced in both TB patients and PPD+ people. Short term cell lines (STCL) specific for these peptides proliferated in vitro and secreted IFN-${\gamma}$ upon antigenic stimulation in PPD+ donors. Lastly, HLA-$A^*0201$ dimer assays indicated that $PstA1_{75-83}$ specific CD8+ T cell population in PPD+ healthy donors is heterogeneous since approximately 25~33% of $PstA1_{75-83}$ specific CD8+ T cell population in PPD+ healthy donors produced IFN-${\gamma}$ upon peptide stimulation. Conclusion: Our results suggest that MHC class I restricted CD8+ T cell mediated immune responses to M. tuberculosis infection are induced in both TB patients and PPD + people; however, the CD8+ T cell population is functionally heterogeneous.

Role of N-terminal Hydrophilic Amino Acids in Molecular Translocation of CTLA-4 to Cell Surface (CTLA-4 항원의 세포막 도달 기작에서 친수성 N말단 아미노산 잔기의 역할)

  • Han, Ji-Woong;Lee, Hye-Ja;Kim, Jin-Mi;Choi, Eun-Young;Chung, Hyun-Joo;Lim, Soo-Bin;Choi, Jang-Won;Chung, Yong-Hoon
    • IMMUNE NETWORK
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    • v.2 no.2
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    • pp.102-108
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    • 2002
  • Background: This study was aimed to differentiate two forms of CTLA-4 (CD152) in activated peripheral blood lymphocyte and clarify the mechanism how cytoplasmic form of this molecule is targeted to cell surface. Methods: For this purpose we generated 2 different anti-human CD152 peptide antibodies and 5 different N'-terminal deletion mutant CTLA4Ig fusion proteins and carried out a series of Western blot and ELISA analyses. Antipeptide antibodies made in this study were anti-CTLA4pB and anti-CTLA4pN. The former recognized a region on extracellular single V-like domain and the latter recognized N'-terminal sequence of leader domain of human CD152. Results: In Western blot, the former antibody recognized recombinant human CTLA4Ig fusion protein as an antigen. And this recognition was completely blocked by preincubating antipeptide antibody with the peptide used for the antibody generation at the peptide concentration of 200 ug/ml. These antibodies were recognized human CD152 as a cytoplasmic sequestered- and a membrane bound- forms in phytohemagglutinin (PHA)-stimulated peripheral blood lymphocyte (PBL). These two forms of CD152 were further differentiated by using anti-CTLA4pN and anti-CTLA4pB antibodies such that former recognized cytosolic form only while latter recognized both cytoplasmic- and membraneforms of this molecule. Furthermore, in a transfection expression study of 5 different N'-terminal deletion mutant CTLA4Ig, mutated proteins were secreted out from transfected cell surface only when more than 6 amino acids from N'-terminal were deleted. Conclusion: Our results implies that cytosolic form of CTLA-4 has leader sequence while membrane form of this molecule does not. And also suggested is that at least N'-terminal 6 amino acid residues of human CTLA-4 are required for regulation of targeting this molecule from cytosolic- to membrane- area of activated human peripheral blood T lymphocyte.

AN EXPERIMENT STUDY ON THE BOND STRENGTH OF ADHESIVE RESINS TO SUEEXSSIVELY RECAST ALLOYS FOR REISN-BONDED RESTORATIONS (반복주조된 치과용 합금의 피착면 처리방법에 따른 접착성 수지와의 접착강도에 관한 실험적 연구)

  • Jung, Kum Tai;Yang, Jae Ho;Lee, Sun Hyung;Jung, Hun Yung
    • The Journal of Korean Academy of Prosthodontics
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    • v.28 no.2
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    • pp.53-76
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    • 1990
  • The purpose of this study was to evaluate the tensile bond strength of adhesive resins to successively recast Rexillium III and Degudent-U. Recasting was done 4times successively. Specimen $A_1$, were cast by new metal, and $A_2$ by surpus of $A_1$, $A_3$ by surplus of, $A_2$ $A_4$ by surplus of $A_3$, $A_5$ by surplus of $A_4$ plus 50% new metal. The types of surface treatment for resinbonded restoration in this experiment were electrolytic etching by OXY-ETCH(Oxy dental products, Inc., Hillside, New Jersey, U.S,A.), aluminum oxide blasting, anodic oxidation by EZ-OXISOR( Towagiken Co., Kyoto, Japan), electrotinplating by Kura Ace(Kuralay Co., Kyoto, Japan). Three kinds of cementing resin used in this study were Comspan(K.P. Cauil Co, Milford Delaware, U.S.A.), Super Bond C&B(Sun-Medical Co. Ltd., Kyoto,Japan), Panavia EX(Kuralay Co., Ltd., Osaka, Japan). Tensile bond strength was measured by Instron Universal testing machineModel 1125) and all the specimen were observed with SEM(JEOL, JSM-T2000) and mode of bond failure were recorded. The obtained results were as follows : 1. In electrolytic etched group, tensile bond strength was decreassed when recast alloy was used, and tensile bond strength of Compan and panavia EX were not significantly different(P>0.05). 2. In remaining group treated by aluminum oxide blasting, EZ-OXIOR, Kura Ace, tensile bond strength were not changed when recast alloy were used, and tensile bond strength of SuperBond(C&B and Panavia EX were not significantly different(P>0.05). 3. IN SEM evaluation, electrolytic etched group and electrotinplated group exhibited different image when recast alloy was used, and remaining groups treated by aluminum oxide blasting, EZ-OXISOR exhibited the same. 4. IN observation of bond failure, electrolytic etched group exhibited adhesive failure and remaing groups treated by aluminium oxid blasting, EZ-OXISOR, Kura Ace exhibited adhesive and cohesive failure.

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