• 대한세포병리학회:학술대회논문집
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• 1993.06a
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• pp.27-27
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• 1993

• Korean Journal of Food Science and Technology
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• v.17 no.2
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• pp.101-106
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• 1985

Dried soymilk residue(SMR) which was prepared by washing with ethanol and acetone, followed by drying at $60^{\circ}C$ was investigated for its sorption characteristics and the relationship between moisture content and water activity when it was mixed with wheat flour. During storage at $20^{\circ}C$ and various RH, an excess sorption phenomena was observed for solvent treated and dried SMR before equilibrium reached. A simple equation of log(dw/dt)=b log t+log a, where t is storage time(hr) and w is %$(H_2O)$ was drived for sorption or desorption rate of dried SMR at RH range of 12%-92%. From sorption isothermifigure, the moisture content(Mi) could be calculated from water activity by the equation of Mi=b Aw+a. The proposed equation was proved to have better fitness than those of the Smith isotherm equation or Lang and Steinberg equation for the mixture of dried SMR and wheat flour.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.6
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• pp.3681-3684
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• 2013

Deregulated miRNAs participate in osteosarcoma genesis. In this study, the expression of miRNA-218 in human osteosarcomas, adjacent normal tissues and Saos-2 human osteosarcoma cells was first assessed. Then the precise role of miRNA-218 in osteosarcoma cells was investigated. Upon transfection with a miR-218 expression vector, the proliferation of Saos-2 human osteosarcoma cells determined using the ATPlite assay was significantly suppressed, whilw migration of Saos-2 cells detected by wound healing and invasion determined using transwells were dramatically inhibited. Potential target genes of miR-218 were predicted and T-cell lymphoma invasion and metastasis 1 (TIAM1) and matrix metalloproteinase 2 (MMP2) and 9 (MMP9) were identified. This was confirmed by western blotting, which showed that miR-218 expression inhibited TIAM1, MMP2 and MMP9 protein expression. Collectively, these data suggest that miR-218 acts as a tumor suppressor in osteosarcomas by down-regulating TIAM1, MMP2 and MMP9 expression.

• Proceedings of the Korea Contents Association Conference
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• 2014.06a
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• pp.297-298
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• 2014

• TTA Journal
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• s.112
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• pp.133-135
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• 2007

• Communications of the Korean Institute of Information Scientists and Engineers
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• v.32 no.6
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• pp.25-30
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• 2014

• IMMUNE NETWORK
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• v.9 no.2
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• pp.58-63
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• 2009

Background: T cell immunoglobulin and mucin domain containing 3 protein (Tim-3) expressed on terminally differentiated Th1 cells plays a suppressive role in Th1-mediated immune responses. Recently, it has been shown that N-glycosylation affects the binding activity of the Tim-3-Ig fusion protein to its ligand, galectin-9, but the binding properties of non-glycosylated Tim-3 on $CD4^+CD25^+$T cells has not been fully examined. In this study, we produced recombinant Tim-3-Ig fusion proteins in different cellular sources and its N-glycosylation mutant forms to evaluate their binding activities to $CD4^+CD25^+$T cells. Methods: We isolated and cloned Tim-3 cDNA from BALB/C mouse splenocytes. Then, we constructed a mammalian expression vector and a prokaryotic expression vector for the Tim-3-Ig fusion protein. Using a site directed mutagenesis method, plasmid vectors for Tim-3-Ig N-glycosylation mutant expression were produced. The recombinant protein was purified by protein A sepharose column chromatography. The binding activity of Tim-3-Ig fusion protein to $CD4^+CD25^+$T cells was analyzed using flow cytometry. Results: We found that the nonglycosylated Tim-3-Ig fusion proteins expressed in bacteria bound to $CD4^+CD25^+$T cells similarly to the glycosylated Tim-3-Ig protein produced in CHO cells. Further, three N-glycosylation mutant forms (N53Q, N100Q, N53/100Q) of Tim-3-Ig showed similar binding activities to those of wild type glycosylated Tim-3-Ig. Conclusion: Our results suggest that N-glycosylation of Tim-3 may not affect its binding activity to ligands expressed on $CD4^+CD25^+$T cells.

• Nutrition Research and Practice
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• v.8 no.1
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• pp.33-39
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• 2014

Obesity occurs when a person's calorie intake exceeds the amount of energy burns, which may lead to pathologic growth of adipocytes and the accumulation of fat in the tissues. In this study, the effect and mechanism of pear pomace extracts on 3T3-L1 adipocyte differentiation and apoptosis of mature adipocytes were investigated. The effects of pear pomace extract on cell viability and the anti-adipogenic and proapoptotic effects were investigated via MTT assay, Oil red O staining, western blot analysis and apoptosis assay. 3T3-L1 preadipocytes were stimulated with DMEM containing 10% FBS, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), $5{\mu}g/ml$ insulin and $1{\mu}M$ dexamethasone for differentiation to adipocytes. 3T3-L1 cells were cultured with PBS or water extract of pear pomace. Water extract of pear pomace effectively inhibited lipid accumulations and expressions of PPAR-${\gamma}$ and $C/EBP{\alpha}$ in 3T3-L1 cells. It also increased expression of p-AMPK and decreased the expression of SREBP-1c and FAS in 3T3-L1 cells. The induction of apoptosis was observed in 3T3-L1 cells treated with pear pomace. These results indicate that pear pomace water extract inhibits adipogenesis and induces apoptosis of adipocytes and thus can be used as a potential therapeutic substance as part of prevention or treatment strategy for obesity.

• Journal of Korean Public Health Nursing
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• v.20 no.1
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• pp.69-78
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• 2006

Purpose: This study investigated the relationship between knowledge and attitude about smoking of college student smokers. Methods: The data were collected from the 6th to 27th of April 2005, from 411 college student smokers in Jeollabuk-Do area. The collected data were analysed by using the SPSS WIN 11.0. Results: 1) The focus group showed mean $17.40{\pm}12.45$ in the knowledge about smoking. The knowledge about smoking was significantly different among general subject characteristics of age(F=4.84, p=.008), grade(t=2.96, p=.003), major(F=5.93, p=.001), and religion(t=2.96, p=.003). 2) The focus group showed mean $23.69{\pm}16.19$ in their attitude about smoking. The attitude about smoking was significantly different among general subject characteristics of age(F=8.15, p=.000), grade(t=-2.33, p=.020), major(F=8.35, p=.000), and residence(t=3.08, p=.010). 3) The correlations between knowledge and attitude about smoking were positively correlated(p<0.01). Conclusion: The subjects knowledge about smoking was correlated with their attitude about smoking. Therefore, we need to develop various differential smoking prevention programs and extend the role of experts.

• Imaging Science in Dentistry
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• v.41 no.4
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• pp.151-153
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• 2011

Purpose : The purpose of this study was to obtain the description of the mandibular bone quality of male and female patients between 40-60 years old and their differences based on mandibular cortical bone thickness measured using Mental Index (MI). Materials and Methods : Forty digital panoramic radiographs, which consisted of twenty male and twenty female patients, 40-60 years old, were observed. Mandibular cortical bone thickness was measured using MI on both sides of the mandible. The average MI score of two groups were then assessed using t-sample independent test. Results : There were significant differences of mandibular bone quality based on mandibular cortical bone thickness measurement using MI between male and female patients (p<0.05). Conclusion : Mandibular bone quality based on cortical bone thickness measurement using MI of male and female patients indicated a significant difference.