• Title/Summary/Keyword: $Ru_{dpp}$

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The Concentration-Dependent Distribution of Tris(4,7'-diphenyl-1,10'-phenanthroline) Ruthenium (II) within Sol-Gel-Derived Thin Films

  • Lee, Joo-Woon;Cho, Eun-Jeong
    • Bulletin of the Korean Chemical Society
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    • v.32 no.8
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    • pp.2765-2770
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    • 2011
  • Organic dye-doped glasses, viz., ruthenium (II) tris(4,7'-diphenyl-1,10'-phenanthroline) $[Ru(dpp)_3]^{2+}$ incorporated into thin silica xerogel films produced by the sol-gel method, were prepared and their $O_2$ quenching properties investigated as a function of the $[Ru(dpp)_3]^{2+}$ concentration (3-400 ${\mu}M$) within the xerogel. The ratio of the luminescence from the $[Ru(dpp)_3]^{2+}$-doped films in the presence of $N_2$ and $O_2$ ($I_{N2}/I_{O2}$) was used to describe the film sensitivity to $O_2$ quenching. ($I_{N2}/I_{O2}$ changed three-fold over the $[Ru(dpp)_3]^{2+}$ concentration range. Time-resolved intensity decay studies showed that there are two discrete $[Ru(dpp)_3]^{2+}$ populations within the xerogels (${\tau}_1$ ~ 300 ns; ${\tau}_2$ ~ 3000 ns) whose relative fraction changes as the $[Ru(dpp)_3]^{2+}$ concentration changes. The increased $O_2$ sensitivity that is observed at the higher $[Ru(dpp)_3]^{2+}$ concentrations is a manifestation of a greater fraction of the 3000 ns $[Ru(dpp)_3]^{2+}$ species (more susceptible to $O_2$ quenching). A model is presented to describe the observed response characteristics resulting from $[Ru(dpp)_3]^{2+}$ distribution within the xerogel.

Development of a pH/dissolved- oxygen Monitoring System Using HPTS and Rudpp (HPTS, Rudpp를 활용한 pH 및 용존산소 모니터링 시스템 연구)

  • Dong Hyuk Jeong;Daewoong Jung
    • Journal of Sensor Science and Technology
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    • v.32 no.2
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    • pp.82-87
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    • 2023
  • This study proposes a pH-dissolved-oxygen monitoring system using 8-HydroxyPyrene-1,3,6-trisulfonic acid Trisodium Salt (HPTS) and tris(4,7-diphenyl-1,10-phenanthroline)Ruthenium(II) chloride (Rudpp). Commercial water-quality sensors are electrochemical devices that require frequent calibration and cleaning, are subject to high maintenance costs, and have difficulties conducting measurements in real-time. The proposed pH-dissolved-oxygen monitoring system selects a thin-film sensing layer to measure the change in fluorescence intensity. This change in fluorescence intensity is based on reactions with hydrogen ions in an aqueous solution at a given pH and specific amount of dissolved oxygen. The change in fluorescence intensity is then measured using light-emitting diodes and photodiodes in response to HPTS and Rudpp. This method enables the development of a relatively small, inexpensive, and real-time measureable water-quality measurement system.

Development of Optical Fiber Glucose and Lactate Biosensors for Bioprocess Monitoring (생물공정 모니터링을 위한 광섬유 포도당 및 젖산 센서의 개발)

  • Jung, Chang Hwan;Sohn, Ok-Jae;Rhee, Jong Il
    • KSBB Journal
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    • v.32 no.1
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    • pp.35-45
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    • 2017
  • In this work the optical fiber glucose and lactate biosensors were developed by using fluorescent dye and enzyme immobilized on the end tip of an optical fiber. 3-Glycidyloxypropyl)methyldiethoxysilane (GPTMS), (3-Aminopropyl) trimethoxysilane (APTMS) and Methyltrimethoxysilane (MTMS) were used to immobilize glucose oxidase (GOD), lactate oxidase (LOD) and ruthenium(II) complex (tris(4,7-diphenyl-1,10-phenanthroline) ruthenium(II), $Ru(dpp)_3^{2+}$) as oxygen sensitive fluorescent dye. MTMS sol-gel was an excellent supporting material for the immobilization of $Ru(dpp)_3^{2+}$, GOD, and LOD on the optical fiber. Storage stability of the optical fiber glucose sensor was kept constant over 20 days, while the optical fiber lactate sensor had constant storage stability over 17 days. The optical fiber glucose and lactate biosensors also maintained good operational stability for 20 hours and 14 hours, respectively. The activities of the immobilized enzymes were most excellent at pH 7 and at $25^{\circ}C$. On-line monitoring of glucose and lactate in a simulated process was performed with the optical fiber glucose and lactate biosensors. On-line monitoring results were agreed with those of off-line data measured with high performance liquid chromatography (HPLC).

Development and Characterization of Optical Dissolved Oxygen Sensor based on the Fluorescence Detection (형광검출기반 광학식 용존산소 측정센서 개발 및 특성 분석)

  • Kwak, Hyun Min;Kwon, Myeunghoi;Choi, Gyewoon;Jung, Yoonseok;Jung, Changhwan;Park, Kiuha;Sohn, Okjae;Kim, Junhyong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.1
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    • pp.569-574
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    • 2014
  • We developed and evaluated a fluorescence-based optical DO sensor (OS-100, Global Optical Communication Ltd., Korea) for long-term monitoring of the dissolved oxygen concentration in waste water treatment. Fluorescent sensing membrane containing $Ru(Dpp)_3{^{2+}}$ (tris(4,7diphenyl-1, 10-phenanthroline) ruthenium(II)) was prepared with GA sol-gel matrix and coated on a quartz plate by sprayed method. Properties of sensor film exhibit deviation about ${\pm}1%$ under wide range of DO concentration from 3 to 10. The developed optical DO sensor was actually mounted in waste water from dyeing industry and successfully applied for on-line DO monitoring. Online monitoring results showed the changes of DO concentrations in wastewater treatment processes with accuracy better than ${\pm}2%$ during the 6 months measurements period in vicious environmental conditions.

Study on Online Monitoring of Dissolved Oxygen, pH and Cell Concentration in E. coli Cultivation Processes Using MABOOMSTM (마이크로플레이트 기반 생물반응기 시스템 (MABOOMSTM)을 이용한 대장균 배양공정에서 용존산소, pH 및 세포농도의 온라인 모니터링 연구)

  • Sohn, Ok-Jae;Rhee, Jong Il
    • KSBB Journal
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    • v.28 no.1
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    • pp.24-30
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    • 2013
  • Dissolved oxygen, pH and cell concentration have been online monitored in cultivation processes with Escherichia coli by using a $MABOOMS^{TM}$ (microplate-based bioreactor with optical online monitoring systems). Fluorescent sensing membranes containing Ru ${(dpp)_3}^{2+}$ or HPTS were prepared with GA sol-gel matrix and coated into a well of a 24-well microplate. Fluorescence intensity was measured and correlated to the dissolved oxygen or pH. Cell concentrations were also online monitored by measuring optical reflectance at 650 nm. A well of a 24-well microplate could also be divided into 4 parts, each of which was coated with fluorescent sensing membranes for the detection of dissolved oxygen or pH. The 24-well microplate coated with fluorescent sensing membranes or a 4-divided sensing membrane. was used to online monitor the dissolved oxygen, pH and cell concentration during E. coli cultivations. The online monitoring results showed the characteristics of cell growth in cultivation processes very well.