• Asian-Australasian Journal of Animal Sciences
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• v.15 no.9
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• pp.1341-1347
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• 2002

An experiment was conducted to determine effects of oral administration of $Diakur^{TM}$ (an additive of glucose and electrolytes for young calves) on growth performance and some physiological responses in male broilers reared in a high temperature. A 2 by 3 factorial arrangement test of 2 temperatures (24 and $36^{\circ}C$) and 3 levels of oral administration of the glucose and electrolytes additive, $Diakur^{TM}$, (0, 150 and 300 mg/day/100 gBW) were applied in the experiment. Male broiler chicks (2 weeks of age) were assigned to six groups and received dietary and temperature treatments for 7 days. The additive of glucose and electrolytes was suspended with water and intubated into crop twice a day (08:00 and 17:00). Oral administration of the additive prevented decreases in food intake and growth rates in broilers due to exposure of the hot environment. Oral administration of the additive also improved a lowered electrolyte ($Na^+$ + $K^+$ - $Cl^-$) balance in plasma, low mitogenic response of blood mononuclear cell and an increase in glucose concentration due to exposure to the high environmental temperature. Oral administration of the additive increased rectal temperature regardless of environmental temperatures. On the other hand, blood pH, $pCO_2$ and $HCO_3$ - concentration, and plasma creatine kinase activity were not affected by the oral administration. The results suggested that oral administration of the glucose and electrolytes additive, $Diakur^{TM}$ during heat stress did not only prevent decrease in growth performance, but also normalized some physiological and immunological responses in male broilers.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.2
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• pp.135-143
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• 2014

Exopeptidase active fractions from the hepatopancreas of the Argentina shortfin squid Illex argentinus, were obtained with acetone (AC 30-40%), ammonium sulfate (AS 60-70% saturation), anion exchange chromatography (AE-II, 0.2 M NaCl) and gel filtration chromatography (GF-I, 30-50 kDa) fractionation methods. A bitter peptide solution that has a bitterness equivalent to that of 2% glycylphenylalanine and prepared by tryptic hydrolysis of milk casein, was treated with the exopeptidase active fractions. The GF-I fraction was the best based on aminopeptidase activity (35.3 U/mg), percentage of recovery (30.7%) and a sensory evaluation (1.7). The amount of released amino acids increased as incubation time increased, and the bitterness of the enzyme reaction mixtures decreased. Incubation with the GF-I fraction for 24 h resulted in the hydrolysis of several peptides as revealed by the reverse-phase high performance liguid chromatography profile, with three peaks (3, 5 and 6) decreasing in area (%) and three peaks (1, 2 and 4) increasing in area (%). Therefore, the GF-I fraction appeared to be ideally suited to reduce bitterness in protein hydrolysates by catalyzing the hydrolysis of bitter peptides.

• Microbiology and Biotechnology Letters
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• v.15 no.6
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• pp.369-374
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• 1987

A bacterium which grows on D-$\alpha$-Amino-$\varepsilon$-Caprolactam as sole carbon, energy and nitrogen source was isolated from the sludge of industrial areas in Taegu, and identified as Alcaligenes eutrophus. The optimum pH, temperature and concentration of D-$\alpha$-Amino-$\varepsilon$-Caprolactam for the growth were 6.0, 3$0^{\circ}C$ and 0.2% respectively. The bacteria could utilize glucose and fructose as a carbon source, and utilize ammonium chloride, ammonium nitrate, ammonium sulfate and sodium nitrate as a nitrogen source, and utilize L-Iysine and L-glutamate as a carbon and nitrogen source. It was found with thin layer chromatography and polarimeter that D-$\alpha$-Amino-$\varepsilon$-Caprolactam was converted to L-Iysine by the cell-free extracts of Alcaligenes eutrophus A52.

• Applied Biological Chemistry
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• v.39 no.2
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• pp.99-103
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• 1996

A bacterial strain producing a novel endo-inulinase, hydrolysing inulin into oligosaccharides was isolated from soil and identified as Arthrobacter sp. S37 The enzyme production was induced by inulin and jerusalem artichoke extract. The maximum enzyme production was obtained with medium containing 1.5% jerusalem artichoke extract, 1.0% yeast extract, $0.5%\;NaNO_3,\;0.05%\;MgSO_4{\cdot}7H_2O,\;0.05%\;KCl,\;0.0016%\;FeCl_3{\cdot}6H_2O\;and\;0.05%\;KH_2PO_4$. The optimum temperature and pH for the enzyme production were $30^{\circ}C$ and 8.0, respectively. Under the optimum condition, the enzyme activity in the culture broth reached at maximum, 10.8 units/ml after cultivation for 24 hours.

• Carbon letters
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• v.15 no.1
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• pp.57-66
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• 2014

Three activated carbons (ACs) were prepared using NaOH (N) as an activating agent. Hydrofluoric acid pre-leached rice husk was used as a precursor. After leaching, the precursor was washed with distilled water, dried, crushed, and then sieved; a size fraction of 0.3-0.5 mm was selected for carbonization in the absence of air at $600^{\circ}C$. The carbonization product (LC) was mixed with NaOH at ratios of 1:2, 1:3, and 1:4 (wt of LC: wt of NaOH) and the produced ACs after activation at $800^{\circ}C$ were designated NLC21, NLC31, and NLC41, respectively. Surface and textural properties were determined using nitrogen adsorption at $-196^{\circ}C$, scanning electron microscopy images, thermogravimetric analysis, and Fourier transform infrared spectra. These ACs were used as adsorbents for lead(II) from aqueous solutions. The effects of the textural properties and the chemistry of the carbon surfaces were investigated and the impact of the operation conditions on the capacity for lead(II) sorption was also considered. Modification of NLC41 with $H_2O_2$ and $HNO_3$ gave two other adsorbents, $H_{NLC41}$ and $N_{NLC41}$ respectively. These two new samples exhibited the highest removal capacities for lead(II), i.e.117.5 and 128.2 mg/g, respectively. The adsorption data fitted the Langmuir isotherm and the kinetic adsorption followed pseudo-second order kinetics. The thermodynamic parameters have been determined and they indicated a spontaneous endothermic process.

• Bulletin of the Korean Chemical Society
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• v.34 no.11
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• pp.3307-3311
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• 2013

A new fiber optical sensor was developed for the determination of 2,4-dichlorophenol (DCP). The sensor was based on DCP oxidation by oxygen with the catalysis of iron(III) tetrasulfophthalocyanine (Fe(III)PcTs). The optical oxygen sensing film with $Ru(bpy)_3Cl_2$ as the fluorescence indicator was used to determine the consumption of oxygen in solution. A lock-in amplifier was used for detecting the lifetime of the oxygen sensing film by measuring the phase delay change of the sensor head. The different variables affecting the sensor performance were evaluated and optimized. Under the optimal conditions (i.e. pH 6.0, $25^{\circ}C$, Fe(III)PcTs concentration of 0.62 mg/mL), the linear detection range and response time of the sensor are $1.0{\times}10^{-6}-9.0{\times}10^{-6}$ mol/L and 250 s, respectively. The sensor displays high selectivity, good repeatability and stability, and can be used as an effective tool in analyzing DCP concentration in practical samples.

• Bulletin of the Korean Chemical Society
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• v.24 no.3
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• pp.363-368
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• 2003

The major ionic components of precipitation collected at the 1100 Site of Mt. Halla and Jeju city have been determined. The reliability of the analytical data was verified by the comparison of ion balances, electric conductivities and acid fractions; all of their correlation coefficients were above 0.94. Ionic strengths lower than $10^{-4}$ M were found in 53% of the 1100 Site samples and 28% of the Jeju city samples. Compared with other inland areas, the wet deposition of $Na^+,\;Cl^-\;and\;Mg^{2+}$ was relatively larger, but that of $NH_4^+,\;nss-SO_4^{2-}$(non-sea salt sulfate) and $NO_3^-$ was lower. Especially the wet deposition increase of $Ca^{2+}$ in the spring season supports the possibility of the Asian Dust effect. The acidification of precipitation was caused mostly by $SO_4^{2-}\;and\;NO_3^-$ in the Jeju area, and the organic acids have contributed only about 7% to the acidity. The neutralization factors by NH₃were 0.47 and 0.48, and that of CaCO₃was 0.31 and 0.25 at the 1100 Site and Jeju city, respectively. Investigation into major influencing sources on precipitation components by factor analysis showed that the precipitation at the 1100 Site had been influenced mostly by an anthropogenic source, followed by soil and seawater sources. The precipitation at Jeju city was mainly influenced by oceanic sources, followed by anthropogenic and soil sources.

• Journal of Ecology and Environment
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• v.30 no.3
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• pp.271-276
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• 2007

A simple and efficient protocol was developed for culturing Cu-tolerant and Cu-accumulating plants via pre-adaptation to Cu during plant tissue culture. We induced multiple shoots from begonia (Begonia evansiana Andr.) leaf explants on MS medium supplemented with naphtaieneacetic acid and benzyladenine. After 3 months, small plantlets were transferred to MS medium supplemented with $100{\mu}M\;CuCl_2$ for pre-adaptation to Cu and cultured for 5 months. Then, these plantlets were individually planted in pots containing artificial soil. An additional 500 mg of Cu dissolved in 1/4 strength MS solution was applied to each pot during irrigation over the course of 2 months. We planted pre-adapted and control begonias in soil from the II-Kwang Mine, an abandoned Cu mine in Pusan, Korea, to examine their ability to tolerate and accumulate Cu for phytore-mediation. Pre-adapted begonias accumulated $1,200{\mu}g$ Cu/g dry root tissue over the course of 45 days. On the other hand, non-Cu-adapted controls accumulated only $85{\mu}g$ Cu/g dry root tissue. To enhance Cu extraction, chelating agents, ethylenediamine tetraacetic acid (EDTA)-dipotassiun and pyridine-2,6-dicarboxylic acid (PDA), were applied. While the chelating agents did not enhance accumulation of Cu in the roots of control begonias, EDTA application increased the level of Cu in the roots of pre-adapted begonias twofold (to $2,500{\mu}g$ Cu/g dry root tissue). Because pre-adapted begonias accumulated a large amount of Cu, mainly in their roots, they could be used for phytostabilization of Cu-contaminated soils. In addition, as a flowering plant, begonias can be used to create aesthetically pleasing remediation sites.

• Journal of Preventive Medicine and Public Health
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• v.26 no.2 s.42
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• pp.231-250
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• 1993

Thirty five male Sprague-Dawley rats were treated with cadmium chloride solution ranging from 0.2 to 3.2mg $CdCl_2/kg$ by intravenous single injection. At 48 hours after administration of cadmium, total cadmium, MT bound cadmium and histopathologic finding in liver, kidney, lung, heart, testis, metallothionein in liver, kidney and total cadmium in bleed were examined. Tissue cadmium concentration was highest in liver, followed by in kidney, heart, lung and testis. Cadmium bound to rnetallothionein (MT-Cd) and ratio of MT-Cd to total cadmium were increased in liver and kidney dependently of cadmium exposure dose, but not significantly changed in other organs. On histopathologic finding, the most susceptible organ was heart in considering cadmium exposed dose, but testis in considering cadmium concentration. Blood cadmium concentration was increased with dose-dependent pattern, and significantly correlated with tissue cadmium concentration, so that we may estimate tissue cadmium concentration by measurement of blood cadmium concentration. Metallothionein in liver and kidney was increased with dose-dependent pattern, higher in liver than in kidney, and was significantly correlated with tissue cadmium concentration. However, metallothionein induction efficiency of tissue cadmium(${\mu}g\;MT/{\mu}g\;Cd$) was eater in liver than in kidney, and reverse to tissue concentration or exposed dose of cadmium.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.5
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• pp.336-340
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• 2009

The issue of acid precipitation and related environmental problems in East Asia has been emerging. To evaluate the acidity and chemical characteristics of rainwater in Korea, its chemical properties during cultivation season from April to October were investigated at Taean in 2007. Also, to estimate the contribution of ions on its acidity, ion composition characteristics and neutralization effects by cation ions were determined. The ion balance between cations and anions values showed high correlation. The mean values of pH and EC were 4.9 and $32.9{\mu}S\;cm^{-1}$, respectively. The EC of rainwater showed seasonal characteristic, which was $91.4{\mu}S\;cm^{-1}$ with relatively low rainfall compared with other monitoring periods. $Na^+$ was the main cation followed by $NH_4{^+}$ > $Ca^{2+}$ > $H^{+}$ > $Mg^{2+}$ > $K^+$. Among these ions, $Na^{+}$ and $NH_4{^+}$ covered over 70% of total cations. In the case of anion, the order was $SO_4{^{2-}}$ > $NO_3{^-}$ > $Cl^{-}$. The mean value of sulfate, which is main anion component in the samples was $152.1{\mu}eq\;L^{-1}$. Also, 90% of soluble sulfate in rainwater was $nss-SO_4{^{2-}}$(non-sea salt sulfate). With fractional acidity and theoretical acidity of rainwater samples, $NH_4{^+}$ and $Ca^{2+}$ contributed greatly in neutralizing the rain acidity.