• 기술사
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• 제32권4호
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• pp.104-118
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• 1999

Alaska Pollack (Theragramma), Mackerel (Socomber japonicus), yellow corvenia (Pseudosc iance manchurica) were dressed, and then meat was separated from the other parts through a fish meat separator. After dehydration, the meat was ground with a silent cutter, packaged in polyethylene bags, and stored at -15。C for days. Samples were taken at regular intervals throughout the storage period and investigated for changes in puality characteristics such as the amounts of nitrogenous compounds, degree of lipid oxidation, fatty acid composition, and organoleptic factors. The resrlts obtained in this study were as follows; 1. The volatile basic nitrogen[VBN] contents of Alaska pollack, and yellow corvenia meat pastes increased from 14.4, 11.2 and 10.8mg% to 41.6, 38.3 and 40.6mg%, respectively during a 120 day storage period, whereas the trimethylamine oxide nitrogen [TMAO-N] contents decreased from 117.2, 12.8 and 17.2mg% to 40.3, 2.6 and 7.1mg% during the same period. 2. The TBA value of the mackerel meat paste showed a maximum peak after 60 days, and then decreased gradually, whereas the TBAvalues of the alaska pollack and yellow corvenia meat pastes increased steadily during the same period. The acid values of the meat pastes increased during the storage period, while the iodine values decreased. 3. The fatty acid composition of the total lipid of the meat pastes changed considerably during ghe 120 days storage period : saturated fatty acids in the total lipid such as myristic, palmitic, and stearic acid increased, while unsaturated fatty acids such as linoleic, gadoleic, eicosapentaenoic, erucic and docosahexaenoic acid decreased steadily. The initial percentage contents of the unsaturated fatty acids in the total lipid of the Alaska pollack, mackerel, and yellow corvenia meat pastes were 87.2%, 63.9%, and 75.9% respectively. However, the contents decreased to 46.0%, 42.5% and 51.3% after the 120day storage period. 4. The color of the meat pastes changde gradually into dark brown. L values of the meat paste measured with a thistimulus colorimeter decreased steadily during the storage period, while a and b values increased during same period. 5. Judging from the results of organoleptic evaluation on the fish odor, color and overall acceptability, significant difference were found between the odor and color of the mackerel and those of the yellow corvenia meat pastes. Overall acceptability score of yellow corvenia was higher than that of Alaska pollack or mackerel meat pastes.

• Journal of Acupuncture Research
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• 제18권1호
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• pp.76-87
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• 2001

Objective : Zusanli(ST36) and Hoku(Li4) are analgesic acupuncture points frequently used for acupuncture in Oriental medicine. The present study was conducted to see the antinociceptive effects produced by electroacupuncture combined two frequencies(Low, High) and two different acupuncture points(LI4, ST36) in the rat tail flick test. Method : In this study the Rats (Sprague-Dawley, 250-300g) were partially anesthetized with thiopental sodium(40mg/kg, i.p.). The basal reaction time for the tail-flick was 3${\pm}$0.5 sec. Low frequency(3Hz, 5V, biphasic) and high frequency(100Hz, 5V, biphasic) were applied to the inserted needle for the period of insertion(twenty minutes). Experimental groups are divied as follow; a) electroacupuncture stimulation groups at Hoku with or high frequency(L-EA, H-EA), b) electroacupuncture stimulation groups at Zusanli with low or high frequency(1-EA, h-EA), c) low frequency at Hoku and Zusanli(LIEA), d) low frequency at Hoku and high frequency at Zusanli(LhEA), e) high frequency at Hoku and low frequency at Zusanli(HIEA), f) high frequency at Hoku and Zusanli(HhEA) Results : The individual stimulation at either Hoku or Zusanli with low frequency has stronger and longer analgesic effect than high frequency stimulation. In addition, the combined stimulation at Hoku and Zusanli with low frequency has superior effect to individual stimulation with low frequency. LhEA and LIEA have superior effect to other stimulation groups among the combined groups. In order to determine the involvement of opioid system on the different antinociceptive effects, Naloxone, an opioid antagonist, was used in the combined groups. LIEA is the most sensitive when naloxone was administrated among study groups. HhEA is the least sensitive in the administration of naloxone. Conclusion : From results, this study confirmed that the opioid system is involved in analgesic effect of low frequency stimulation of acupuncture point, and we also can suggest the stronger analgesic effect of combining stimulation points is due to the theory of spatial summation in the nervous system.

• 한국식품저장유통학회지
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• 제13권4호
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• pp.483-489
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• 2006

홍삼과 청국장의 단일 및 혼합 분말의 향미특성을 분석하기 위하여, 원료와 제조회사가 다른 홍삼(J사 6년근, P인삼조합 5, 6년근)과 청국장($A{\sim}E$사 제품) 단일분말 및 혼합분말의 색도, 휘발성 성분 및 관능적기호도를 조사하였다. 홍삼과 청국장 분말의 색상은 시료에 따라 약간의 차이를 보였으며, 홍삼의 경우 L값은 $75.56\sim85.50$, a값은 $1.90\sim6.30$, b값은 $23.29\sim35.08$이었고 청국장의 경우 L값은 $64.96\sim71.69$, a값은 $4.64\sim8.30$, b값은 $30.45\sim36.50$을 각각 나타내었다. 홍삼과 청국장 분말의 휘발성 성분은 시료별 차이를 보였으나 홍삼분말에서 주요성분으로는 hexanal, $\beta-pinene$, methyl benzene, 3,5-methyl propyl nonane, 2-propanone, decane, 2,8-dimethyl undecane 등이, 청국장분말에서는 2,3-butanedione, decane, 2,2,7,7-tetramethyl octane, 3-methyl butanal 등이 확인되었다. 홍삼과 청국장 혼합분말의 휘발성 성분의 양은 홍삼의 혼합양이 적어지고 청국장의 혼합양이 많아질수록 감소하는 경향이었다. 혼합분말의 관능적기호도는 홍삼과 청국장 분말의 동량 혼합일 경우가 가장 좋게 평가되었으며, 이 경우 주요 휘발성 성분은 2-propanone, 2-butanone, pentane, hexanal, 3-methyl butanal 등이었다. 이로써 홍삼과 청국장 분말의 향미특성은 제조조건에 따라 달라지며, 두 분말의 적정 혼합을 통하여 새로운 가공소재의 제조가 가능한 것으로 확인되었다.

• 한국조리학회지
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• 제20권4호
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• pp.127-143
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• 2014

본 연구에서는 기능성 물질이 함유된 유색보리와 유색보리 분말을 첨가한 식빵 반죽 및 식빵을 제조하여 이화학적, 관능적 특성을 첨가량을 달리하여 측정하였다. 유색보리 분말의 수분함량은 9.35%, 조단백은 9.37%, 조지방은 1.64%, 조회분은 2.96%로 나타났다. 수용성 식이섬유 함량은 3.21 g/100 g, 불용성 식이섬유 함량은 4.91 g/100 g, 총 식이섬유 함량은 8.12 g/100 g으로 나타났고, ${\beta}$-glucan 함량은 49.31 mg/g으로 나타났다. DPPH radical 소거능은 56.76%로 나타났고, 총 페놀 함량은 234.34 mg/100g으로 나타났다. 유색보리 분말을 첨가한 식빵 반죽의 farinograph 측정 결과 consistency, water absorption, development time, time breakdown은 첨가량이 증가할수록 감소하였고, tolerance index는 첨가량이 증가할수록 증가하였다. Alveogram 측정 결과 overpressure P값, extensibility L값, swelling index G값, deformation energy W값은 첨가량이 증가할수록 감소하였다. Amylograph 측정 결과 pasting temp. T값은 첨가량이 증가할수록 증가하였고, peak viscosity P값, hot past viscosity H값, breakdown P-H값은 첨가량이 증가할수록 감소하였다. 유색보리 분말을 첨가한 식빵의 굽기 손실률, 비용적은 첨가량이 증가할수록 감소하였다. 색도 L값, b값은 첨가량이 증가할수록 감소하였고, a값은 첨가량이 증가할수록 증가하였다. 유색보리 분말 첨가한 식빵의 texture 경도, 점착성은 첨가량이 증가할수록 증가하였고, 응집성, 탄력성, 씹힘성은 첨가량이 증가할수록 감소하였다. 기호도는 맛, 부드러움, 씹힘성은 10% 첨가군이 높게 나타났고, 전반적인 기호도도 10% 첨가군이 5.04로 가장 높게 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제23권10호
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• pp.1299-1307
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• 2010

This study was conducted to evaluate the effect of dietary antioxidant and energy density on performance and antioxidative status in transition cows. Forty cows were randomly allocated to 4 dietary treatments in a $2{\times}2$ factorial design. High or low energy density diets (1.43 or 1.28 Mcal $NE_L$/kg DM, respectively) were formulated with or without antioxidant (AOX, a dry granular blend of ethoxyquin and tertiary-butylhydroquinone; 0 or 5 g/cow per d). These diets were fed to cows for 21 days pre-partum. During the post-partum period, all cows were fed the same lactation diets, and AOX treatment followed as for the pre-partum period. Feeding a high energy diet depressed the DMI, milk yield, and 4% fat-corrected milk (FCM) of cows. However, AOX inclusion in the diet improved the milk and 4% FCM yields. There was an interaction of energy density by AOX on milk protein, milk fat and total solids contents. Feeding a high energy diet pre-partum increased plasma glucose and ${\beta}$-hydroxybutyrate, whereas dietary AOX decreased plasma ${\beta}$-hydroxybutyrate value during the transition period. There were also interactions between time and treatment for plasma glutathione peroxidase activity and malondialdehyde content during the study. Cows fed high energy diets pre-partum had higher plasma glutathione peroxidase activity 3 days prior to parturition, compared with those on low energy diets. Inclusion of AOX in diets decreased plasma glutathione peroxidase activity in cows 3 and 10 days pre-partum. Addition of AOX significantly decreased malondialdehyde values at calving. Energy density induced marginal changes in fatty acid composition in the erythrocyte membrane 3 days post-partum, while AOX only significantly increased cis-9, trans-11 conjugated linoleic acid composition. The increase in fluidity of the erythrocyte membrane was only observed in the high energy treatment. It is suggested that a diet containing high energy density pre-partum may negatively affect the anti-oxidative status, DMI and subsequent performance. Addition of AOX may improve the anti-oxidative status and reduce plasma ${\beta}$-hydroxybutyrate, eventually resulting in improved lactation performance; the response to AOX addition was more pronounced on the high energy diet.

• 대한본초학회지
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• 제23권4호
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• pp.21-29
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• 2008

Objectives: To determine the standards for discrimination of Magnoliae Cortex, the experiment of specific external-internal characters and the physicochemical pattern analysis were performed. Methods: External characteristics was observed using a stereoscope. Paraffin-mediated sectioned materials were stained by Ju's method. Physicochemical patterns of materials were analyzed using HPLC. Results: 1. Botanical characteristics: Magnolia officinalis had one seed and a white flower, while M. obovata had two seeds and a white flower. Machilus thunbergii had berry and spherical fruits and yellowish green panicles. 2. External characteristics: M. officinalis and M obovata were dark and thick. M. officinalis was gray brown and greasy while M. obovata was light-gray, less oily and smoothly sectioned. Machilus thunbergii was thin and relatively light or yellow-brown, coarsely sectioned and faintly specific scents. 3. Internal characteristics: The bast parts of M. officinalis and M. obovata were commonly wider than Machilus thunbergii The cork cortex of M. officinalis was $10{\sim}mg/L$ cell layers with many oil cells, while that of M. obovata was $4{\sim}7$ cell layers with less oil cells. Machilus thunbergii's xylem which consisted of ring-shaped cambium at 1st and 2nd part was occupied in large portion. 4. Physicochemical pattern: Both M. officinalis and M. obovata involved honokiol and magnolol. All kinds of M. officinalis involved Magnatriol B but one kind of M. ovobata and all of Machilus thunbergii didn't. Machilus thunbergii showed different pattern of chromatogram from that of 2 species above. Conclusions: These results could be used as standards for discrimination of Magnoliae Cortex and as the method of objectification in medicinal herbs giving the basic resource for bioactivity research.

• 대한수의학회지
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• 제37권3호
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• pp.569-576
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• 1997

Fowl typhoid(FT) caused by Salmonella gallinarum is an infectious, egg-transmitted disease and characterized by swollen bronze liver, greenish-yellow diarrhea and high mortality in growing and adult chickens. Since 1992 the outbreak of FT has been increased. Several problems have been occurred such as absence of appropriate vaccines and lack of useful therapeutic methods. In these studies we investigated the pathogenicity of S gallinarum isolated in chickens. To compare the pathogenicity among the species of chickens, all chickens were challenged intramuscularly or orally with $1{\times}10^7$ CFU of S gallinarum. The brown-colored layers were more susceptible and white leghone chickens were more resistant than other species. In the brown layer chickens orally challenged, lethal doses ($LD_{50}$) of the isolates were inoculated at 1 day, 2 weeks, 4 weeks and 8 weeks old chickens with amount of $10^{4.2}$, $10^{4.7}$, $10^{7.0}$ and $10^{7.6}$ CFU, respectively. The chickens which were intramuscularly challenged with the less amount than $10^2$ CFU showed higher mortality than that of the chickens orally inoculated with same dose. Also, we investigated the recovery rates of bacteria from various organs of survival chickens which were challenged orally with $5{\times}l0^7$ CFU of S gallinarum. The bacteria was more frequently and isolated earlier from the liver and spleen than from any other ogans. In the pathogenicity test, the white-leghorn chickens which were known as resistant-strain against Salmonella were artificially immunosuppressed using bursectomy and/or dexamethasone treatment. Mortality of chickens with both bursectomized and treated with dexamethasone was higher(90%) than that of the control group(10%), the bursectomized chickens(10%) and the dexamethasone only treated group(20%). It was suggested that the protective mechanism in chickens against S gallinarum may be required both the functions of B-cells and T-cells.

• Restorative Dentistry and Endodontics
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• 제31권4호
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• pp.269-281
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• 2006

2.9%의 과산화수소를 함유한 시험용 부착형 미백제 (Medison dental whitening strip, Samsung medical Co., Anyang, Korea)를 실험군 피험자 23명, 시험용 미백제에서 과산화수소를 제외한 동일한 제재를 대조약으로 대조군 피험자 24명에게 매일 1시간 30분씩, 2주간 적용하여 임상적 효능과 안전성을 평가하였다. 미백 효과는 Shade Vision, Chroma Meter 및 Vitapan classical shade guide를 이용하여 색변화를 측정하여 다음과 같은 결론을 얻었다. 1. 실험군에서의 전체 치아의 색변화량 $({\Delta}E^{*})$은 세 가지 측정법에서 모두 대조군에 비해 통계적으로 유의한 차이를 보였고 (p < 0.05), 쉽게 인지할 수 있는 정도였다. 2. 색변화는 색좌표 상에서 병도의 증가와 녹색조 및 청색조로의 변화에 의해 얻어졌다. 3. 치아별로는 어두운 견치의 색변화량이 가장 컸고, 측절치, 중절치 순으로 감소하였다. 4. 47명의 피험자 중 5명이 경도의 과민증을 경험하였으나 곧 완화되었고, 치은염증지수와 전기치수검사 결과 시험 전후에 차이가 없어 안전성을 확인하였다.

• 한국식품영양과학회지
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• 제33권6호
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• pp.1006-1012
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• 2004

시판되는 새우젓을 종류별(오젓, 육젓, 추젓, 자하젓, 새하젓), 제조자별(재래, 기업), 원산지별(국산, 중국산, 베트남산)로 구입하여 그 이화학적, 관능적 특성을 분석, 비교하였다. 기업 새우젓은 염도, VBN및 AN등 이화학적 특성들이 제품별로 거의 일정하였던 반면, 재래 새우젓은 제품별로 차이가 많았다. 기업 새우젓은 재래 새우젓에 비해 염도가 높았던 반면, VBN 및 AN 함량은 낮은 경향을 나타내 낮았다. 재래새우젓은 그 종류별로 다양한 특성을 나타내었는데, 대체적으로 기업 새우젓보다 VBN 및 AN 함량이 높게 나타났다. 새우젓의 VBN 및 AN 함량의 차이는 새우의 어획 및 제조시기의 차이에 따른 차이보다는 그 숙성 정도의 차이로 사료된다. 수입산 추젓의 경우 다른 새우젓에 비해 높은 염도와 낮은 VBN, AN 함량을 보였다. 육젓의 L값과 b값이 높은 반면, 자하젓, 새하젓은 a값이 높았다. 관능검사 결과 재래 새우젓은 기업 새우젓에 비해 외관의 수용도는 낮았으나, 감칠맛, 전반적인 맛, 전반적인 수용도의 점수가 높았다. 새우젓의 종류별로 볼 때, 재래 및 기업 새우젓 모두에서 육젓의 관능평가 점수가 높았으며, 자하젓과 새하젓은 전반적인 맛 및 감칠맛 정도의 점수가 높게 나타났다. 주성분 분석 결과, 주성분 PC1에 대해 재래 새우젓에서는 각각 새하젓, 오젓, 자젓이 기업새우젓에서는 육젓이 그리고 주성분 PC2에 대해서는 재래새우젓의 경우 자젓, 육젓이 기업새우젓에서는 추젓이 양의 방향으로 부하 되고 있었다. 또한 전반적인 수용도와 가까이 위치한 이화학적 특성으로는 재래새우젓에서는 a값, TBA, AN값이었으며, 기업새우젓에서는 L값과 b값이었다.

• 한국식품과학회지
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• 제51권3호
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• pp.221-226
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• 2019

쌀가루 첨가면에 패각분말을 0.1, 0.2, 0.3, 0.4%로 수준으로 첨가하여 최적 패각분말 첨가량을 조사하고, 밀가루 알카리제 첨가면과 품질 특성에 관하여 비교하였다. 면에 쌀가루를 첨가할 경우 밀가루면 대비 L값과 b값이 높아지는 경향을 보여 면의 외관이 밝아지는 것을 확인 하였다. 쌀가루를 첨가면의 조리 특성은 면의 수분흡수율이 높아져 수율 향상이 이루어질 수 있으나, 조리 과정 중 탁도도 같이 높아져 면의 용출 작용이 병행되는 것을 알 수 있었다. 패각분말의 첨가량이 높아질수록 면의 색도, pH, 수분흡수율, 탁도가 높아지는 경향을 보였으나, 패각분말의 함량이 0.3%를 초과하게 되면 경도, 탄력성, 씹힘성, 신장성이 떨어지는 경향을 보였다. 이는 알칼리제의 특성으로 일정수준 이상을 첨가하면 면에 형성된 네트워크를 저해하여 면의 조직감을 떨어뜨리는 작용을 하는 것으로 생각된다. 이 연구에서는 쌀가루면에 패각분말 0.2%를 첨가 할 때 알칼리제첨가 밀가루면과 유사한 물성을 나타내었다. 하지만 이 이상의 패각분말을 첨가할 경우 외관은 좋아질 수 있으나, 면의 조직감은 더 떨어지는 것으로 나타났다. 따라서 본 연구결과는 쌀면류 및 합성 첨가물 활용의 기초자료로 제공되어 향후 연구 수행에 도움을 줄 수 있을 것으로 판단됩니다.