• Asian Pacific Journal of Cancer Prevention
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• v.15 no.7
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• pp.3219-3226
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• 2014

Chemotherapy continues to be a mainstay of cancer treatment, although drug resistance is a major obstacle. Lipid metabolism plays a critical role in cancer pathology, with elevated ether lipid levels. Recently, alkylglyceronephosphate synthase (AGPS), an enzyme that catalyzes the critical step in ether lipid synthesis, was shown to be up-regulated in multiple types of cancer cells and primary tumors. Here, we demonstrated that silencing of AGPS in chemotherapy resistance glioma U87MG/DDP and hepatic carcinoma HepG2/ADM cell lines resulted in reduced cell proliferation, increased drug sensitivity, cell cycle arrest and cell apoptosis through reducing the intracellular concentration of lysophosphatidic acid (LPA), lysophosphatidic acid-ether (LPAe) and prostaglandin E2 (PGE2), resulting in reduction of LPA receptor and EP receptors mediated PI3K/AKT signaling pathways and the expression of several multi-drug resistance genes, like MDR1, MRP1 and ABCG2. ${\beta}$-catenin, caspase-3/8, Bcl-2 and survivin were also found to be involved. In summary, our studies indicate that AGPS plays a role in cancer chemotherapy resistance by mediating signaling lipid metabolism in cancer cells.

• Journal of Chest Surgery
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• v.22 no.2
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• pp.256-264
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• 1989

Modified Blalock-Taussig operation remains the standard technique of systemic-to-pulmonary artery shunt in patients of congenital heart disease with decreased pulmonary blood flow. We reviewed the 41 patients who had been performed modified Blalock-Taussig operation from March 1985 to Feb. 1988, and angiographically measured pulmonary artery size before and after shunt, and calculated pulmonary artery index [PAI] and ratio of left and right pulmonary artery to descending aorta[[LPA+RPA]/dAo]. The mean duration of palliation after shunt operation was 624 days and mean age of the patient at shunt operation was 3.59 years. Mean PAI increased significantly from 131.15*67.11 mm2/M2 preoperatively to 232.70*84.46 mm2/M2 postoperatively. Mean ratio of right and left pulmonary artery to descending aorta also increased significantly from 1.48*0.40 preoperatively to 1.92*0.50 postoperatively. All patients manifested clinical improvement; there was mean decrease in hematocrit of 8.95%, mean increase in arterial oxygen saturation of 11.08%. Pulmonary arterial growth was not influenced by age at operation, initial pulmonary artery size, or graft size, but significantly influenced by antegrade flow. The patients who have some antegrade flow were in more increase of PAI. There were no linear correlation between change of PAI, change of [[LPA+RPA]/dAo], SaO2, and duration. But, according to Scatterplot between change of PAI and duration, some complex correlation was suggested and mean PAI was decreased after 2-year palliation. We concluded that modified Blalock-Taussig operation is excellent palliative surgery for pulmonary artery growth especially on the patient who have some antegrade flow, and the proper duration of palliation was about 2 years.

• Proceedings of the SCSK Conference
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• 2003.09b
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• pp.73-86
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• 2003

Endothelins secreted from keratinocytes are intrinsic madiators for human melanocytes in UVB-induced pigmentation. Antimelanogenic ingredient, 1,2-Ο-diferulylglycerol(SM709) isolated from bamboo extract inhibited the melanin synthesis of Bl6F10 melanoma cells by 62%. To understand the cellular mechanism of antimelanogenic activity of SM709 in human melanocytes, the effects of SM709 on the ET-l-induced $Ca^{2+}$ mobilization were investigated. ET-l receptors in human melanocytes were characterized by using specific antagonist and found that ET-l increased intracellular $Ca^{2+}$ by activating ET-B receptor. SM709 completely blocked the ET-l-induced intracellular $Ca^{2+}$ increase and its inhibitory effect showed dose- and time- dependent manners. To investigate the role of SM709 on intracellular $Ca^{2+}$ store, when the $Ca^{2+}$ store was partially depleted by thapsigargin; a specific inhibitor of ER-type $Ca^{2+}$-ATPase, caffeine-induced $Ca^{2+}$ mobilization did not changed in the presence or absence of SM709, suggesting that SM709 has no effect on the $Ca^{2+}$ store. It is known that LPA receptor and P$_2$ receptor are linked to InsP$_3$ second messenger system. When these receptors in melanocytes were activated by LPA and ATP, the intracellular $Ca^{2+}$ signaling was observed even in the presence of SM709. From the above results, it can be suggested that SM709 has an antimelanogenic activity by antagonizing the ET-B receptor, resulting in subsequent intracellular $Ca^{2+}$ signaling, in UV induced pigmentation.nduced pigmentation.

• 한국노년학
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• v.37 no.2
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• pp.349-368
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• 2017

The purpose of this study is to empirically structure the patterns of baby boomer retirees'consumption and their effects on depression in later life. Using the 5th wave of Korea Retirement and Income Study(KReIS) in 2013, of baby boomers born in 1955 to 1963, we analyzed those who were completely retired(n=420). The Latent Profile Analysis(LPA) classified the empirical patterns of consumption and then a multiple regression analyzed the effect of consumption patterns on depression among these baby boomer retirees. The LPA results showed that the consumption patterns consist of five groups: (1) Basic life-oriented class(26.9%), (2) Balanced consumption class(29.3%), (3) Social life-oriented class(18.3%), (4) Leisure-oriented class(18.5%), and (5) Education-oriented class(7.0%). Baby boomer retirees' depression was associated with their consumption patterns. Specifically, the basic life-oriented class showed significantly lower depression than the education-oriented class. Other correlates such as higher education, having no spouse, lower subjective health, greater limitations in physical functions, having a diagnosed disability, having fewer children, higher dissatisfaction with leisure activities, and lower self-perceived economic status were associated with higher levels of depression in this study. These findings suggest meaningful implications for gerontological policy and practice for baby boomer retirees in Korea. In light of vulnerable retirement preparation, social services specialized for baby boomers should consider financial education for revamping consumption scale beyond asset management and long-term case management of pre and post retirement cases.

• Korean Journal of School Psychology
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• v.11 no.1
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• pp.253-274
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• 2014

The present study classified groups based on latent profile of self-determination motivation(amotivation, external motivation, intrinsic motivation), and examined the determinants for each group. The data was collected through panel data of Korea Education Longitudinal Study(KELS), total 5,459 participants who answered questionnaires of self-determination motivation of two times both second grade of middle school and second grade of high school. To identify the change motivational type, standardized residual was conducted using SPSS 17.0., and the latent classes for the change of motivational type was investigated using M-Plus in the frame work of Latent Profile Analysis(LPA). The results indicated that five groups(increase of self-determination, self-determination maintenance, self-determination developmental delay, elf-determination confusion, decrease of self-determination group) were classified based on latent profile. In addition, parental control, academic self-concept, teacher-student relationship, test anxiety, avoidance orientation, gender, father's education, and income were significantly related to each group. Lastly, the implications for directions of the adolescent counseling, limitations and future research are discussed.

• The Journal of the Korea Contents Association
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• v.20 no.9
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• pp.365-379
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• 2020

The purpose of this study identifies the patterns of students' learning motivation profiles on entrepreneurial educational motivation among a sample of university students from one of the Korean national university(n=614). This study also examines the relationship between students' learning motivation profiles and entrepreneurial intention and types of entrepreneurship. In order to explore the types of leaning motivation profiles, a latent profile analysis was employed. Result from LPA revealed five distinct types of learning motivation profiles fit the data best, and these five profiles are compared with students' entrepreneurial intentions and types of entrepreneurship. Results showed that Profiles (profile 5 and 4-high goal orientation) are associated with the higher level of entrepreneurial intention. Regarding the type of entrepreneurship, the majority of all students are interested in individual and co-entrepreneurship with friend regardless of the patterns of profiles.

• Korean Journal of Culture and Social Issue
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• v.27 no.2
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• pp.167-189
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• 2021

The purpose of this study was to classify different profiles of grit (effort vs. interest) and passion mindset (fixed vs. growth) in university students and to understand whether there are differences in psychological characteristics (ie. depression, anxiety and happiness) and career adaptability between the profiles. To this end, we recruited 359 students aged 18 and 25 and applied a Latent Profile Analysis(LPA). The results revealed 4 distinct profiles : Profile1-a burgeoning type, Profile2-an ordinary type, Profile3-a fixed/limited type, Profile4-sprouting/drifting type. The profile1(LP1, 22.2%) had high levels of grit and passion growth mindset. The profile2(LP2, 63.8%) showed average levels in both grit and passion mindset. The profile3(LP3, 8.8%) had a low level of grit and a high level of passion fixed mindset. The profile4(LP4, 5.1%) showed a low level of grit and a high level of passion growth mindset. The four profiles were distinct in psychological characteristics and career adaptability. The burgeoning type(profile1) was most adaptive in comparison to the fixed/limited type(profile 3). These results suggest that grit has more powerful effects on psychological characteristics while passion growth mindset exerts stronger influencers in career adaptability. Putting the results together, our findings imply that both passion growth mindset and grit need to be considered together to enhance the individuals' comprehensive well-being and to increase the level of adaptation for career preparation.

• Journal of Ginseng Research
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• v.47 no.3
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• pp.366-375
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• 2023

Background: Ginseng contains three active components: ginsenosides, gintonin, and polysaccharides. After the separation of 1 of the 3 ingredient fractions, other fractions are usually discarded as waste. In this study, we developed a simple and effective method, called the ginpolin protocol, to separate gintonin-enriched fraction (GEF), ginseng polysaccharide fraction (GPF), and crude ginseng saponin fraction (cGSF). Methods: Dried ginseng (1 kg) was extracted using 70% ethanol (EtOH). The extract was water fractionated to obtain a water-insoluble precipitate (GEF). The upper layer after GEF separation was precipitated with 80% EtOH for GPF preparation, and the remaining upper layer was vacuum dried to obtain cGSF. Results: The yields of GEF, GPF, and cGSF were 14.8, 54.2, and 185.3 g, respectively, from 333 g EtOH extract. We quantified the active ingredients of 3 fractions: L-arginine, galacturonic acid, ginsenosides, glucuronic acid, lysophosphatidic acid (LPA), phosphatidic acid (PA), and polyphenols. The order of the LPA, PA, and polyphenol content was GEF > cGSF > GPF. The order of L-arginine and galacturonic acid was GPF >> GEF = cGSF. Interestingly, GEF contained a high amount of ginsenoside Rb1, whereas cGSF contained more ginsenoside Rg1. GEF and cGSF, but not GPF, induced intracellular [Ca²⁺]_i transient with antiplatelet activity. The order of antioxidant activity was GPF > GEF = cGSF. Immunological activities (related to nitric oxide production, phagocytosis, and IL-6 and TNF-α release) were, in order, GPF > GEF = cGSF. The neuroprotective ability (against reactive oxygen species) order was GEF > cGSP > GPF. Conclusion: We developed a novel ginpolin protocol to isolate 3 fractions in batches and determined that each fraction has distinct biological effects.

• International Journal of Oral Biology
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• v.47 no.2
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• pp.25-31
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• 2022

Lysophosphatidic acid (LPA) is a bioactive lipid messenger involved in the pathogenesis of chronic inflammation and various diseases. Recent studies have shown an association between periodontitis and neuroinflammatory diseases such as Alzheimer's disease, stroke, and multiple sclerosis. However, the mechanistic relationship between periodontitis and neuroinflammatory diseases remains unclear. The current study found that lysophosphatidic acid receptors 1 (LPAR1) and 6 (LPAR6) exhibited increased expression in primary microglia and astrocytes. The primary astrocytes were then treated using medium conditioned to mimic periodontitis through addition of Porphyromonas gingivalis lipopolysaccharides, and an increased nitric oxide (NO) production was observed. Application of conditioned medium from human periodontal ligament stem cells with or without LPAR1 knockdown showed a decrease in the production of NO and expression of inducible nitric oxide synthase and interleukin 1 beta. These findings may contribute to our understanding of the mechanistic link between periodontitis and neuroinflammatory diseases.

• Journal of Chest Surgery
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• v.17 no.1
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• pp.32-40
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• 1984

During the past six years from July 1977 to June 1983, fifteen adult patients of patent ductus arteriosus were surgically treated. The results were as follows: 1. Of the 15 patients, their age range was 17 to 34 years with a mean of 24 years, and sexual predominance was women [9. cf. 6 men]. 2. The most common symptom showed exertional dyspnea, and 10 patients were classed in NYHA class II, the rest were all class III. 3. On physical examination, all patients were auscultated continuous murmur, but concomitantly diastolic murmur was noted apical region in 2 patients. 4. On roentgenogram of chest, normal finding was 3 patients, and the other patients were revealed the evidence of pulmonary congestion. 5. The electrocardiogram was normal in 6 patients, but LVH was seen in 5, and 2 patients were LVH+ LAH. 6. Cardiac catheterization was performed in 12, and mean value of SO2[LPA-RV] was 6.3%, Q/Q 2.09, peak systolic pulmonary arterial pressure 45.3 mmHg, and Rp/Rs 0.365. 7. All operations were carried out by posterolateral thoracotomy. In 6 patients, division and suture of ductus were possible, the other patients were treated by division and ligation with heavy silk or Dacron patch. 8. Postoperative complications were hoarseness, persistent murmur, reoperation for bleeding, and atelectasis. Early and late mortality was 20% [3 patients], and cause of death was mainly aneurysmal rupture of previous operative site.