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Effect of Housing on Physiological Responses and Energy Expenditure of Sheep in a Semi-arid Region of India

  • Bhatta, Raghavendra;Swain, N.;Verma, D.L.;Singh, N.P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.8
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    • pp.1188-1193
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    • 2005
  • An investigation was carried out to study the effect of two housing systems on physiological responses and energy expenditure of sheep in a semi-arid region of India. Two types of housing management were adopted. First was a shed- $6{\times}3\;m^2$ structure with all the four sides of 1.8 m chain link fencing with a central height of 3 m. The roof was covered with asbestos sheets and with mud floorings. Second was an open corral- $6{\times}3\;m^2$ open space with all the four sides covered with 1.8 m chain link fencing. Thirty-four (32 ewes and 2 rams) sheep of native Malpura breed aged about 18 months (body weight 28 kg ewes; 35 kg rams) were grazed together on a 35 ha plot of native range. All the sheep were grazed as a flock from 08.00 to 17.00 h during a yearlong study. The flock was divided into two groups (16 ewes+1 ram) in the evening and housed as per the systems (Shed and Open Corral). Dry and wet temperatures were recorded at 06.00 h and 21.00 h using a wet and dry bulb-thermometer both inside the shed and in the open corral and temperature humidity index (THI) was calculated. There was significant (p<0.05) difference in the THI between shed and open corral in all the seasons, indicating that shed was always warmer compared to open corral. Rectal temperature (RT) of both the groups of sheep was similar during morning as well as evening throughout the seasons. There were significant (p<0.05) differences in the skin temperature (ST) and respiration rate (RR) between the two groups at both the measurements in all the seasons. Highest energy expenditure (EE) was recorded inside the shed at 21.00 h (224 kJ/h) during monsoon and lowest at 6.00 h during winter (119 kJ/h). There was a significant (p<0.05) difference between the EE inside the shed and that in the open corral. It was concluded that housing had significant effects on the physiological responses and EE of sheep. Provision of housing at night was stressful during monsoon (with less rainfall) and summer, whereas it was protecting the sheep from acute cold during winter in a semi-arid region of India.

Storage Conditions and Oviposition Methods for Gryllus bimaculatus (Gryllidae) Eggs (쌍별귀뚜라미(Gryllus bimaculatus) 산란방법 및 알 저장조건)

  • Kim, Seong-Yeon;Kim, Do-Ik;Koo, Hui-Yeon;Kim, Jung-Eun;Kim, Hyun-Jin;Lee, Yu-Beom;Kim, Ji-Soo;Kim, Ho-Hyuk;Han, Yeon-Soo;Kim, Young-Cheol
    • Korean journal of applied entomology
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    • v.59 no.2
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    • pp.133-138
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    • 2020
  • In 2016, the two-spotted cricket was approved as a general food ingredient by the Korean Ministry of Food and Drug Safety. Additionally, the two-spotted crickets have high protein content (60%) and can be reared throughout the year even during the overwintering period. In this study, storage conditions were set in case cricket breeding was not possible due to problems such as breeding space and labor costs, and selection oviposition mats to determine high fecundity rates and low cannibalism rates for the eggs were investigated. The oviposition mat was mostly composed of soil (p < 0.05). Less than 62 crickets per 10 litters were found to be best suited for 1 day of spawning, based on the cannibalism rate of the eggs during the egg laying period. The results from the hatched eggs indicated that the hatching extended 12 to 14 days, and the best hatching rate was approximately 85% when stored at low temperatures(16℃) for 10 days. An efficient production proposed method that established the best oviposition mat and egg storage method for the cricket.

A photoelastic study of the stress distribution on canine retraction by segmented TMA T-loop spring (Segmented TMA T-loop spring에 의한 견치 후방이동시의 응력분포에 관한 광탄성법적 분석)

  • Yoon, Young-Jooh;Kim, Kwang-Won;Yu, Pil-Sik
    • The korean journal of orthodontics
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    • v.31 no.2 s.85
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    • pp.199-207
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    • 2001
  • The segmented TMA T-loop spring, used for reciprocal space closure and described by Burstone, was used to achievebodily movement of canine. Photoelastic analysis is a technique for the transformation of internal stress into visible light patterns. The two-dimensional photoelastic stress analysis was performed, and stress distribution was recorded by photography. The purpose of this study was to visualize photoelastically the distribution of forces transmitted to the alveolus and surrounding structures using new segmented TMA T-loop spring for canine retraction. The results were as follows: 1. Decreased activation produced decreased stress of upper 1st. premolar extraction site and increased intrusive stress of upper 1st. molar, regardless of T-loop position. 2. At 5mm activation, More posterior positioning of T-loop Produced an increased stress in upper 1st. premolar extraction site. 3. At 3mm activation, More posterior positioning of T-loop produced an increased stress in upper 1st. premolar extraction site and mesial lower half of upper 1st. molar mesio-buccal root. 4. At 1mm activation, More anterior positioning of T-loop produced an increased stress in upper mesial and blew apex area of upper canine root. 5. 0.25 B/L ratio and 3mm activation produced bodily movement of canine. To summarize, desired tooth movement and anchorage requirement is possible by altering the activation and mesio-distal position of the T-loop spring.

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Synthesis, Crystal Structure and Theoretical Calculation of a Novel Nickel(II) Complex with Dibromotyrosine and 1,10-Phenanthroline

  • Huang, Guimei;Zhang, Xia;Fan, Yuhua;Bi, Caifeng;Yan, Xingchen;Zhang, Zhongyu;Zhang, Nan
    • Bulletin of the Korean Chemical Society
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    • v.34 no.10
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    • pp.2889-2894
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    • 2013
  • A new complex [$Ni(phen)(C_9H_8Br_2NO_3)_2{\cdot}2CH_3OH{\cdot}2H_2O$] [phen: 1,10-phenanthroline $C_9H_8Br_2NO_3$: 3,5-dibromo-L-tyrosine] was synthesized and characterized by IR, elemental analysis and single crystal X-ray diffraction. X-ray crystallography shows that Ni(II) ion is six-coordinated. The Ni(II) ion coordinates with four nitrogen atoms and two oxygen atoms from three ligands, forming a mononuclear Ni(II) complex. The crystal crystallizes in the Orthorhombic system, space group $P2_12_12$ with a = 12.9546 ${\AA}$, b = 14.9822 ${\AA}$, c = 9.9705 ${\AA}$, V = 1935.2 ${\AA}$, Z = 1, F(000) = 1008, S = 0.969, ${\rho}_{calcd}=1.742g{\cdot}cm^{-3}$, ${\mu}=4.688mm^{-1}$, $R_1$ = 0.0529 and $wR_2$ = 0.0738 for 3424 observed reflections (I > $2{\sigma}(I)$). Theoretical study of the title complex was carried out by density functional theory (DFT) method and the B3LYP method employing the $6-3l+G^*$ basis set. The energy gap between HOMO and LUMO indicates that this complex is prone to interact with DNA. CCDC: 908041.

Flora and Life Form of Habitats for Nannophya pygmaea Rambur (꼬마잠자리 서식지의 식물상과 생활형)

  • Kim, Myung-Hyun;Han, Min-Su;Choi, Chul-Mann;Pang, Hye-Sun;Jung, Myung-Pyo;Na, Young-Eun;Kang, Kee-Kyung
    • Korean Journal of Environmental Agriculture
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    • v.29 no.2
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    • pp.206-213
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    • 2010
  • The aim of this study was to provide basic informations for restoring a habitat of Nannophy pygmaea Rambur. Environments and flora of the habitats were investigated in Youngdong, Sanbuk, and Nongam South Korea. The habitats were abandoned paddy fields with tree-unshaded space and with the water depths of about 2 - 10 cm all the year round. The water quality was recorded 5.44 - 7.33 in pH, 0.017 - 0.480 dS/m in EC, 1.34 - 4.94 mg/L in T-N and 0.06 - 0.46 mg/L in T-P. The pH and EC showed a wide range values, and T-N and T-P showed a high values comparatively. This result implies that a water quality does not important as the condition for a habitat of the species. A total of 86 vascular plant taxa belonging to 35 families, 60 genera, 67 species, 2 subspecies, 14 varieties and 3 forma were recognized in the habitats. Twenty two species (25.6%) overlapped among the habitats; Equisetum arvense, Salix koreensis, Persicaria thunbergii, Stellaria alsine var. undulata, Hypericum laxum, Kummerowia striata, Epilobium pyrricholophum, Oenanthe javanica, Mosla dianthera, Utricularia vulgaris var. japonica, Plantago asiatica, Eupatorium lindleyanum, Juncus diastrophanthus, J. effusus var. decipiens, J. papillosus, Aneilema keisak, Alopecurus aequalis, Arthraxon hispidus, Carex dickinsii, Cyperus sanguinolentus, Eleocharis acicularis for. longiseta and E. congesta. Biological type was determined to be HH-R5-D4-e(t) type; HH 39 taxa (45.3%), R5 42 taxa (48.8%), D4 59 taxa (68.6%) and e (or t) 23 taxa (26.7%).

A MEASUREMENT OF THE COSMIC MICROWAVE BACKGROUND B-MODE POLARIZATION WITH POLARBEAR

  • ADE, P.A.R.;AKIBA, Y.;ANTHONY, A.E.;ARNOLD, K.;ATLAS, M.;BARRON, D.;BOETTGER, D.;BORRILL, J.;CHAPMAN, S.;CHINONE, Y.;DOBBS, M.;ELLEFLOT, T.;ERRARD, J.;FABBIAN, G.;FENG, C.;FLANIGAN, D.;GILBERT, A.;GRAINGER, W.;HALVERSON, N.W.;HASEGAWA, M.;HATTORI, K.;HAZUMI, M.;HOLZAPFEL, W.L.;HORI, Y.;HOWARD, J.;HYLAND, P.;INOUE, Y.;JAEHNIG, G.C.;JAFFE, A.H.;KEATING, B.;KERMISH, Z.;KESKITALO, R.;KISNER, T.;JEUNE, M. LE;LEE, A.T.;LEITCH, E.M.;LINDER, E.;LUNGU, M.;MATSUDA, F.;MATSUMURA, T.;MENG, X.;MILLER, N.J.;MORII, H.;MOYERMAN, S.;MYERS, M.J.;NAVAROLI, M.;NISHINO, H.;ORLANDO, A.;PAAR, H.;PELOTON, J.;POLETTI, D.;QUEALY, E.;REBEIZ, G.
    • Publications of The Korean Astronomical Society
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    • v.30 no.2
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    • pp.625-628
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    • 2015
  • POLARBEAR is a ground-based experiment located in the Atacama desert of northern Chile. The experiment is designed to measure the Cosmic Microwave Background B-mode polarization at several arcminute resolution. The CMB B-mode polarization on degree angular scales is a unique signature of primordial gravitational waves from cosmic inflation and B-mode signal on sub-degree scales is induced by the gravitational lensing from large-scale structure. Science observations began in early 2012 with an array of 1.274 polarization sensitive antenna-couple Transition Edge Sensor (TES) bolometers at 150 GHz. We published the first CMB-only measurement of the B-mode polarization on sub-degree scales induced by gravitational lensing in December 2013 followed by the first measurement of the B-mode power spectrum on those scales in March 2014. In this proceedings, we review the physics of CMB B-modes and then describe the Polarbear experiment, observations, and recent results.

Determination of moisture threshold for solution sampling in different soil texture (토양용액 채취를 위한 토성별 한계수분함량 설정)

  • Lee, Chang Hoon;Kim, Myung Sook;Kong, Myung Seok;Kim, Yoo Hak;Oh, Taek-Keun;Kang, Seong Soo
    • Korean Journal of Agricultural Science
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    • v.41 no.4
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    • pp.399-404
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    • 2014
  • Soil moisture is an important factor for the availability and circulation of nutrients in arable soil. The purpose of this study was to set thresholds moisture content on soil nitrate concentration in the solution for real-time diagnosis. Sandy loam, silt loam, and sandy loam was filled with $1.2g\;cm^{-3}$ at Wagner pots, 0, 100, and $200mg\;L^{-1}$ of $KNO_3$ was saturated. Nitrate in standard solution was recovered about 95% by passing the porous cup. Nitrate concentrations in sampling of soil solution were examined by using a porous cup. The soil solution was higher in accordance with sandy loam> silt loam> clay loam, limited water filled pore space for sampling soil solution was 33.7, 56.4, and 62.2%, respectively. Nitrate concentration in the soil solution was negligible at sandy loam and silt loam during sampling periods, which was decreased about 50~82% in clay loam compared to the initial $NO_3$-N concentration in the saturated $KNO_3$ solution. Over limitation of soil solution sampling, soil EC and $NO_3$-N content were increased with the saturated $NO_3$-N concentration, regardless of soil texture (p<0.05). Conclusively, soil solution by using a porous cup was possible, regardless of the soil texture, which was useful for the diagnosis in nitrate concentration of soil solution. However, because nitrate concentration of soil solution in a clay loam changes, it was necessary for careful attention in order to take advantage for the real-time diagnosis of nitrogen management in soil.

Apoptosis Induced by Adenosine 5'-triphosphate in Mouse Leukemic Cells (Mouse Leukemia 세포에서 Adenosine 5'-triphosphate에 의한 Apoptosis)

  • Joo, Nan-Young;Park, Kyu-Sang;Chung, Hae-Sook;Kong, In-Deok;Lee, Joong-Woo
    • The Korean Journal of Physiology and Pharmacology
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    • v.1 no.6
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    • pp.817-824
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    • 1997
  • Extracellular ATP elicits various biological responses and plays a significant role in physiological regulation. Recently, ATP-induced growth inhibitions were reported in some tumor cell lines, but these effects and mechanisms are not well hewn. This study was conducted to investigate ATP-induced growth inhibition in mouse $leukemic(P388D_1)$ cells. ATP inhibited cell growth in a dose-dependent manner as analyzed by MTS assay$(IC_{50}: 33.1\;{\mu}M)$. Nucleotides other than ATP, such as ADP$(37.5;{\mu}M)$ and AMP$(33.2;{\mu}M)$ had the same effects as ATP but adenosine$(57.8;{\mu}M)$ showed less effect than ATP. ATP attenuated the cells in $G_0/G_l\;and\;G_2/M$ phases but increased those in S phase in flow cytometric analysis. Hypodiploid cells$(A_0)$, the presumptive findings of apoptosis, were found among the ATP-treated cells. ATP induced DNA fragmentation into $180{\mu}200\;bps $as measured by electrophoresis. some apoptotic cells were stained by TUNEL method. ATP increased the intracellular free $Ca^{++}$concentration$([Ca^{++}]_i)$ and the increment of $([Ca^{++}]_i)$ was caused by influx from the extracellular space. These results suggest that extracellular ATP induces growth inhibition through apoptosis.

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Growth and nutrient uptake by Palmaria palmata integrated with Atlantic halibut in a land-based aquaculture system

  • Corey, Peter;Kim, Jang K.;Duston, Jim;Garbary, David J.
    • ALGAE
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    • v.29 no.1
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    • pp.35-45
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    • 2014
  • Palmaria palmata was integrated with Atlantic halibut Hippoglossus hippoglossus on a commercial farm for one year starting in November, with a temperature range of 0.4 to $19.1^{\circ}C$. The seaweed was grown in nine plastic mesh cages (each $1.25m^3$ volume) suspended in a concrete sump tank ($46m^3$) in each of three recirculating systems. Two tanks received effluent water from tanks stocked with halibut, and the third received ambient seawater serving as a control. Thalli were tumbled by continuous aeration, and held under a constant photoperiod of 16 : 8 (L : D). Palmaria stocking density was $2.95kg\;m^{-3}$ initially, increasing to $9.85kg\;m^{-3}$ after a year. Specific growth rate was highest from April to June (8.0 to $9.0^{\circ}C$), 1.1% $d^{-1}$ in the halibut effluent and 0.8% $d^{-1}$ in the control, but declined to zero or less than zero above $14^{\circ}C$. Total tissue nitrogen of Palmaria in effluent water was 4.2 to 4.4% DW from January to October, whereas tissue N in the control system declined to 3.0-3.6% DW from April to October. Tissue carbon was independent of seawater source at 39.9% DW. Estimated tank space required by Palmaria for 50% removal of the nitrogen excreted by 100 t of halibut during winter is about 29,000 to $38,000m^2$, ten times the area required for halibut culture. Fifty percent removal of carbon from the same system requires 7,200 to $9,800m^2$ cultivation area. Integration of P. palmata with Atlantic halibut is feasible below $10^{\circ}C$, but is impractical during summer months due to disintegration of thalli associated with reproductive maturation.

Relationship between White Spot Symptom and Physiological Status of Two Penaeid Shrimps

  • Kim, Su Kyoung;Kim, Myung Seok;Park, Myoung Ae;Kim, Su mi;Jang, In Kwon;Kim, Seok Ryel;Cho, Miyoung
    • Korean Journal of Environmental Biology
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    • v.35 no.4
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    • pp.461-467
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    • 2017
  • Shrimps infected with WSSV(White Spot Syndrome Virus) generally exhibit white spots in their inner space of carapaces as an acute clinical sign. In an effort to identify the correlation between this acute clinical sign and the condition, the index factors (RNA/DNA concentration and ratio, trypsin activity) were analyzed. A total 580 farmed Fenneropenaeus chinensis and 130 Lithopenaeus vannamei were collected from western and southern fifteen outdoor ponds in Korea. The status of the white spot pathology was divided into four stages (stage 0, stage I, stage II, and stage III), in accordance with the clinical signs as to the size and area of white spots. A significant decrease in RNA concentration and RNA/DNA ratio for multi-infected fleshy prawn (WSSV and vibrio sp.) occurred during the stage III (the whole carapace is covered with a white spot). In particular, RNA/DNA ratio was significantly lower as $1.47{\pm}0.04$ than other groups. A similar trend was also found in the single infection (WSSV), but the decrease was less than the multi-infection. In the species comparison, both species were vulnerable to the multi-infection, but L. vannamei was more sensitive than F. chinensis(ANOVA, p<0.05): A significant decrease in RNA concentration and RNA/DNA ratio was first found in stage II for the former species, while it was found in stage III for the latter species. Trypsin activity was also showed a similar tendency with nucleic acid variation. Multi-infected shrimp showed drastically decrease of trypsin activity. According to the results, clinical signs of the white spot under carapace have an only physiological effect on shrimp if they covered entirely with white spots.