• Title/Summary/Keyword: $KI_2{\cdot}KI_{10}$

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Effect of Diethyldithiocarbamate on Radiation-induced Learning and Memory Impairment in Mouse (방사선 유도 학습기억 장애에 대한 diethyldithiocarbamate의 효과)

  • Jang, Jong-Sik;Kim, Jong-Choon;Moon, Chang-Jong;Jung, U-Hee;Jo, Sung-Kee;Kim, Sung-Ho
    • Journal of Radiation Protection and Research
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    • v.37 no.3
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    • pp.123-128
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    • 2012
  • Evidence suggests that even low-dose irradiation can lead to progressive cognitive decline and memory deficits, which implicates, in part, hippocampal dysfunction in both humans and experimental animals. This study examined whether diethyldithiocarbamate (DDC) could attenuate memory impairment, using passive avoidance and object recognition test, and suppression of hippocampal neurogenesis, using the TUNEL assay and immunohistochemical detection with markers of neurogenesis (Kiel 67 (Ki-67) and doublecortin (DCX)) in adult mice treated with gamma radiation (0.5 or 2 Gy). DDC was administered intraperitonially at a dosage of 1,000 $mg{\cdot}kg^{-1}$ of body weight at 30 min. before irradiation. In passive avoidance and object recognition memory test, the mice, trained for 1 day after acute irradiation (2 Gy) showed significant memory deficits compared with the sham controls. The number of TUNEL-positive apoptotic nuclei in the dentate gyrus (DG) was increased 12 h after irradiation. In addition, the number of Ki-67- and DCX-positive cells were significantly decreased. DDC treatment prior to irradiation attenuated the memory defect, and blocked the apoptotic death. DDC may attenuate memory defect in a relatively low-dose exposure of radiation in adult mice, possibly by inhibiting a detrimental effect of irradiation on hippocampal neurogenesis.

1-MCP Improves Display Life in Begonia × hiemalis 'Blitz' and 'Carnival'

  • Kim, Yoon-Jin;Kim, Ki-Sun
    • Horticultural Science & Technology
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    • v.30 no.2
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    • pp.152-157
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    • 2012
  • We investigated the effect of 1-methylcyclopropene (1-MCP) on ethylene production induced by simulated transport stress in $Begonia$ ${\times}$ $hiemalis$ 'Blitz' and 'Carnival' to improve the display life in potted plants. The simulated transportation conditions were imposed for 4 days in simulated export containers with darkness, vibration with continuous shaking ($150{\pm}20$ rpm) on a rotary lab shaker, and low temperature ($12^{\circ}C$). Plants were treated with 1-MCP at three concentrations (5, 25, or 125 $nL{\cdot}L^{-1}$) and for three different periods (0, 6, or 12 hours) before undergoing the simulated transport stress treatments. Treatment with 25 or 125 $nL{\cdot}L^{-1}$ 1-MCP inhibited the abscission of open flowers by more than 40% as compared to the untreated plants. One week after the treatments, the ethylene production decreased in the plants treated with 125 $nL{\cdot}L^{-1}$ 1-MCP for 'Blitz' and 25 $nL{\cdot}L^{-1}$ for 'Carnival'. Ethylene production was correlated with concentration and duration of 1-MCP treatment in 'Blitz', but not in 'Carnival'. To reduce flower abscission and ethylene production, thus improve the display life when plants are exposed to transportation stress, we recommend pre-treatment with 1-MCP before packaging, using concentrations and durations specific to each cultivar, 125 $nL{\cdot}L^{-1}$ for 6 h and 25 $nL{\cdot}L^{-1}$ for 12 hours for 'Blitz' and 'Carnival', respectively.

Vegetative Growth and Flowering of Salvia splendens 'Salsa' in Response to Night Interruption (야파 처리가 살비아 'Salsa'의 영양생장 및 개화에 미치는 영향)

  • Hong, Yoon Yeong;Park, Yu Jin;Kim, Yoon Jin;Kim, Ki Sun
    • Horticultural Science & Technology
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    • v.32 no.4
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    • pp.434-439
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    • 2014
  • This research was conducted to examine the effects of night interruption (NI) at different times on vegetative growth and flowering in Salvia splendens 'Salsa'. Plants were grown in a growth chamber under 9-h photoperiod (short-day, SD) or 9-h photoperiod plus NI with light intensity at $3-5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ photosynthetic photon flux. The NI was applied at 18:00-22:00 HR (NI18), 22:00-02:00 HR (NI22), or 02:00-06:00 HR (NI02). The net photosynthetic rate under NI18, NI22, and NI02 increased by 0.33, 0.16, and $0.13{\mu}mol{\cdot}CO_2{\cdot}m^{-2}{\cdot}s^{-1}$, respectively, during the NI period. Dry weight, plant height, and the number of nodes under NI were not significantly different from those of the plants under SD. However, flowering was earlier by approximately 9.8 days, and the number of flowers increased to 138.7 in NI02 compared to 36.4 flowers under SD at 17 weeks after the treatment. Our results indicate that NI02 was the most effective treatment in promoting flowering. Although vegetative growth of salvia was not increased in response to the increased net photosynthesis, flowering was promoted. Under NI18, NI22, and NI02 treatments, 77.8, 88.9, and 100.0% of salvia plants flowered within 8 weeks, whereas 44.4% of the plants flowered within the same time under SD conditions.

Fabrication of Fibrous A12O3-(m-ZrO2)/t-ZrO2 Composites Having 2, 3-Dimensional Array (2,3차원배열을 갖는 섬유상 A12O3-(m-ZrO2)/t-ZrO2 복합재료의 제조)

  • Kim Ki-Hyun;Lee Byong-Taek
    • Korean Journal of Materials Research
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    • v.15 no.9
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    • pp.608-612
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    • 2005
  • Microstructure and mechanical properties of fibrous $A1_2O_3-(m-ZrO_2)/t-ZrO_2$ composite were investigated depending on the dimensional array. By the change of stacking arrangement of fibrous filaments, 2 and 3-dimensional fibrous composites were successfully obtained without bulk defects such as shrinkage cavity and cracks. The maximum mechanical properties were achieved In the 3-dimensional array composite, due to the fine fibrous and dense microstructure control, in which the values of vickers hardness, fracture toughness and bending strength were about 1507 Hv, $7.2MPa{\cdot}m^{1/2}$ and 650 MPa, respectively.

Night Interruption and Night Temperature Regulate Flower Characteristics in Cymbidium

  • Kim, Yoon-Jin;Park, Chae-Jeong;Rho, Hyung-Min;Kim, Ki-Sun
    • Horticultural Science & Technology
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    • v.30 no.3
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    • pp.236-242
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    • 2012
  • We investigated the influences of night interruption (NI) and night temperature on flowering and flower coloration in Cymbidium. Cymbidium 'Red Fire' and 'Yokihi' were grown under a 9 hours photoperiod (control), a 9 hours photoperiod with NI at a low light intensity (LNI) of 3-7 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, or a 9 hours photoperiod with NI at a high light intensity (HNI) of 120 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ for four hours (22:00-02:00 HR) for 16 weeks during the reproductive growth stage (Experiment 1). Thirty month-old Cymbidium 'Red Fire' plants with initiated flowering buds were placed in four different growth chamber with night temperature set points of 6, 9, 12, or $15^{\circ}C$ for 16 hours (18:00 to 09:00 HR) and a daytime temperature of $25^{\circ}C$ (Experiment 2). In Experiment 1, the numbers of visible buds and flowers increased, and time to flowering decreased in both the LNI and HNI treatments, as compared to the control in both cultivars. Red color in Cymbidium 'Red Fire' increased by both LNI and HNI, as evidenced by an increased $a^*$ in plants grown under these conditions, relative to those grown under the control condition. Number of days to visible buds at 9-$15^{\circ}C$ ranged from 31-34 days, as compared to 39 days at $6^{\circ}C$ in Experiment 2. Although as the temperature increased days to flowering decreased when the plant was grown at $15^{\circ}C$ as compared to 6, 9, or $12^{\circ}C$, the red color ($a^*$) also decreased. The number of flowers and percent flowering increased when the night temperature was maintained higher than $9^{\circ}C$. Therefore, NI treatment and maintaining the night temperature at approximately 9-$12^{\circ}C$ during the winter season after flower spike initiation in the reproductive developmental growth stage improve flower quality and controls flowering time.

Vegetative Growth Characteristics of Phalaenopsis and Doritaenopsis Plants under Different Artificial Lighting Sources

  • Lee, Hyo Beom;An, Seong Kwang;Lee, Seung Youn;Kim, Ki Sun
    • Horticultural Science & Technology
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    • v.35 no.1
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    • pp.21-29
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    • 2017
  • This study was conducted to determine the effects of artificial lighting sources on vegetative growth of Phalaenopsis and Doritaenopsis (an intergeneric hybrid of Doritis and Phalaenopsis) orchids. One - month - old plants were cultivated under fluorescent lamps, cool - white light - emitting diodes (LEDs), or warm - white LEDs at 80 and $160{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. The blue (400 - 500 nm) : green (500 - 600 nm) : red (600 - 700 nm) : far - red (700 - 800 nm) ratios of the fluorescent lamps, cool-white LEDs, and warm-white LEDs were 1 : 1.3 : 0.8 : 0.1, 1 : 1.3 : 0.6 : 0.1, and 1 : 2.7 : 2.3 : 0.4, respectively. Each light treatment was maintained for 16 weeks in a closed plant-production system maintained at $28^{\circ}C$ with a 12 h photoperiod. The longest leaf span, as well as the leaf length and width of the uppermost mature leaf, were observed in plants treated with warm-white LEDs. Plants grown under fluorescent lamps had longer and wider leaves with a greater leaf span than plants grown under cool-white LEDs, while the maximum quantum efficiency of photosystem II was higher under cool-white LEDs. The vegetative responses affected by different lighting sources were similar at both 80 and $160{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Leaf span and root biomass were increased by the higher light intensity in both cultivars, while the relative chlorophyll content was decreased. These results indicate that relatively high intensity light can promote vegetative growth of young Phalaenopsis plants, and that warm - white LEDs, which contain a high red-light ratio, are a better lighting source for the growth of these plants than the cool-white LEDs or fluorescent lamps. These results could therefore be useful in the selection of artificial lighting to maximize vegetative growth of Phalaenopsis plants in a closed plant - production system.

Effective Fungicides on Control of Stem and Root Rot of Paprika Caused by Nectria haematococca (Nectria haematococca에 의한 착색단고추 줄기 및 과실썩음병의 약제방제)

  • Jee Hyeong-Jin;Shim Chang-Ki;Ryu Kyoung-Yul;Nam Ki-Woong
    • Research in Plant Disease
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    • v.11 no.2
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    • pp.179-184
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    • 2005
  • A previous study reported that the stem and root rot of paprika (Capsicum annum L. var, grossum) caused by Nectria haematococca became a threat to safe cultivation of the plant in the country. However, no strategies for control the disease have been suggested. In this study, fungicides registered for pepper were screened to evaluate their control effects on the disease. Among fungicides tested, prochloraz manganase complex com pletely suppressed mycelial growth of the pathogen at 10 ppm a.i. tebuconazole, benomyl, and carbendazim $\cdot$kasugamycin also effectively inhibited mycelial growth of the fungus. However, kresoxim-methyl and triflox ystrobin did not suppress mycelial growth but significantly suppressed conidial germination of the fungus. Azoxystrobin, benomyl, prochloraz, tebuconazol, and carbendazim$\cdot$ kasugamycin were also effective to retard conidial germination. In vivo tests, tebuconazole strongly inhibited the plant growth even at 16,000x (15.6 ppm a.i.), while others did not induce chemical injury at 4,000x or 8,000x when drenched into a rockwool cube. In a greenhouse test, prochloraz manganase complex at 125 ppm a.i. (4,000x) showed highest control value by $89.9\%$. Other fungicides thiophanate-methylthiram, axozystrobin, trifloxystrobin, and benomyl presented $60-80\%$ control value in the hydroponic cultivation system. However, application time and interval remained to be investigated for identify maximum residue limit.

Quality Changes in 'Elliot' Blueberries and 'Sulhyang' Strawberries Packed with Two Different Packaging Materials during Refrigerated Storage (기체투과도가 다른 포장재로 포장한 '엘리오트' 블루베리와 '설향' 딸기의 냉장 저장 중 품질 변화)

  • Jung, Seung Hun;Kang, Ji Hoon;Park, Seung Jong;Seong, Ki Hyun;Song, Kyung Bin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.6
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    • pp.901-908
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    • 2014
  • Blueberries and strawberries are highly perishable and easily contaminated with microorganisms. To maintain the quality of these commodities during refrigerated storage, the effects of two packaging materials as well as passive modified atmosphere packaging on the quality of blueberries and strawberries were investigated. The harvested blueberries and strawberries were first treated with combined non-thermal treatment of aqueous chlorine dioxide and fumaric acid, followed by packaging with polyolefin film (6,000 mL $O_2/m^2{\cdot}24hr{\cdot}atm$ at $24^{\circ}C$) and polyamide/polyamide/polyethylene film (PA/PA/PE, 60 mL $O_2/m^2{\cdot}24hr{\cdot}atm$ at $24^{\circ}C$), respectively. After combined sanitizer treatment, the populations of total aerobic bacteria in blueberries and strawberries were reduced by 2.50 and 1.97 log CFU/g while those of yeast and molds were reduced by 1.95 and 2.18 log CFU/g, respectively, compared with the control. In particular, microbial growth in these samples packed with PA/PA/PE film was reduced during refrigerated storage. In addition, the blueberries and strawberries packed with PA/PA/PE film underwent lower weight loss than those packed with polyolefin film during storage. These results suggest that appropriate packaging with proper gas permeability is necessary to maintain the quality of blueberries and strawberries during refrigerated storage.

Isolation and Phylogenetic Characteristics of Exopolysaccharide Producing Bacteria in a Rhizosphere Soil of Medicinal Herbs (약초 근권토양 내 다당 생성세균 분리 및 계통학적 특성)

  • Lee, Hae-Ran;Kim, Ki-Kwhang;Whang, Kyung-Sook
    • Korean Journal of Microbiology
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    • v.46 no.3
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    • pp.278-285
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    • 2010
  • We examined the distribution of exopolysaccharide (EPS) producing bacteria population in rhizosphere soils of domestic medicinal herbs; Angelica sinensis, Atractytodes japonica, Achyranthes japonica, Anemarrhena asphodeloides, and Astragalus membranaceus. Fifty-six percent of the total isolates from rhizosphere soil of Angelica sinensis were EPS producing bacteria, suggesting the dominance of EPS producing bacteria in rhizosphere soil of Angelica sinensis. EPS producing bacteria were enumerated in root system (rhizosphere soil, rhizoplane, inside of root) of Angelica sinensis. Bacterial density of rhizosphere soil, rhizoplane, and inside of root were distributed $9.0{\times}10^6CFU/g{\cdot}soil$, $7.0{\times}10^6CFU/g{\cdot}soil$, and $1.4{\times}10^3CFU/g{\cdot}soil$, respectively. EPS producing bacteria from rhizosphere soil were categorized into five major phylogenetic groups: Alphaproteobacteria (4 strains), Betaproteobacteria (6 strains), Firmicutes (2 strains), Actinobacteria (3 strains), and Bacteroidetes (1 strain) subdivisions. Also, the EPS producing isolates from rhizoplane were distributed as 7 strains in Alphaproteobacteria, 3 strains in Betaproteobacteria, 2 strains in Actinobacteria, 3 strains in Bacteroidetes, and 1 strain in Acidobacteria subdivisions. All of the EPS producing bacteria inside of root belong to genus Chitinophaga. Burkholderia caribiensis DR14, Terriglobus sp. DRP35, and Rhizobium hainanense SAP110 were selected in 112 EPS producing bacteria. These appeared to have produced high levels of exopolysaccharide 6,555 mpa.s, 3,275 mpa.s, and 1,873 mpa.s, respectively. The purified EPS was analyzed Bio-LC. As neutral sugars, glucose, galactose, mannose were detected and as amino sugars, galactosamine and glucosamine were detected. Especilally, analysis of Bio-LC showed that Rhizobium hainanense SAP110 produced glucose (60~89%) and glucosamine (8.5%) as major neutral sugar and amino sugar, respectively.

Removal of nitrate by electrodialysis: effect of operation parameters

  • Park, Ki Young;Cha, Ho Young;Chantrasakdakul, Phrompol;Lee, Kwanyong;Kweon, Ji Hyang;Bae, Sungjun
    • Membrane and Water Treatment
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    • v.8 no.2
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    • pp.201-210
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    • 2017
  • We investigated the effect of applied voltage and electrolyte concentration on the nitrate removal and its energy/current efficiency during the electrodialysis. The current increased as the applied voltage increased up to 30 V showing the limiting current density around 20 V. The nitrate removal efficiency (31 to 71% in 240 min) and energy consumption (11 to $77W{\cdot}h/L$) gradually increased as the applied voltage increased from 10 to 30 V. The highest current efficiency was obtained at 20 V. The increase in electrolyte concentration from 100 to 500 mM led to the dramatic increase of nitrate removal efficiency with much faster removal kinetics (100 % in 10 min).