• Title/Summary/Keyword: $I_c$ degradation

검색결과 397건 처리시간 0.026초

Bacillus subtilis LYH201균주의 섬유소 분해효소의 유전자 Cloning 및 특성분석 (Gene Cloning of Cellulose Degradation Enzyme of Bacillus subtilis LYH201 Strain)

  • 이영한;박상렬
    • 한국토양비료학회지
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    • 제34권5호
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    • pp.333-341
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    • 2001
  • 퇴비화 촉진 미생물인 Bacillus subtilis LYH201균주가 분비하는 섬유소 분해효소를 분자생물학적으로 연구한 결과는 다음과 같다. 섬유소를 분해하는 유전자는 유전자은행에 의해 구한 약 5,000개의 clone 중 CMC 배지 상에서 활성을 가지는 clone을 선발하여 bglC(pLYH7-39)로 명명하였다. 섬유소를 분해하는 bglC 유전자는 Pvu II, EcoRI, SspI의 제한효소 site를 가지고 있었으며, BglC는 Clostridium acetobutylicum GUN_CLOAB(P15704)와 57%의 identity와 71%의 homology를 나타내어 상동성이 가장 높았으며, CMC-SDS-PAGE 분석으로 56 kDa의 분자량을 나타냈고, 온도는 $50^{\circ}C$, pH는 7에서 활성이 가장 높았다.

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원전 가압기수위신호 고장검출 및 검증에 관한연구 (A Study on the Failure Detection and Validation of Pressurizer Level Signal in Nuclear Power Plant)

  • 오성헌;김대일;주운표;정윤형;임장현;윤원영;김건중
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1995년도 추계학술대회 논문집 학회본부
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    • pp.175-177
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    • 1995
  • The sensor signal validation and failure detection system must be able to detect, isolate, and identify sensor degradation as well as provide a reconstruction of the measurements. In this study, this is accomplished by combining the neural network, the Generalized Consistency Checking(GCC), and the Sequential Probability Ratio Test(SPRT) method in a decision estimator module. The GCC method is a computationally efficient system for redundant sensors, while the SPRT provides the ability to make decisions based on the degradation history of a sensor. The methodology is also extended to the detection of noise degradation. The acceptability of the proposed method is demonstration by using the simulation data in safety injection accident of nuclear power plants. The results show that the signal validation and sensor failure detection system is able to detect and isolate a bias failure and noise type failures under transient conditions. And also, the system is able to provide the validated signal by reconstructing the measurement signals in the failure conditions considered.

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Cloning and Sequence Analysis of the xyIL Gene Responsible for 4CBA-Dihydrodiol Dehydrogenase from Pseudomonas sp. S-47

  • 박동우;이상만;가종옥;김지경
    • 미생물학회지
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    • 제38권4호
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    • pp.275-275
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    • 2002
  • Pseudomonas sp. S-47 is capable of catabolizing 4-chlorobenzoate (4CBA) as carbon and energy sources under aerobic conditions via the mesa-cleavage pathway. 4CBA-dioxygenase and 4CBA-dihydrodiol dehydrogenase (4CBA-DD) catalyzed the degradation af 4CBA to produce 4-chlorocatechol in the pathway. In this study, the xylL gene encoding 4CBA-DD was cloned from the chromosomal DNA of Pseudomonas sp. S-47 and its nucleotide sequence was analyzed. The xylL gene was found to be composed of 777 nucleotide pairs and to encode a polypeptide of 28 kDa with 258 amino acid residues. The deduced amino acid sequence of the dehydrogenase (XylL) from strain S-47 exhibited 98% and 60% homologies with these of the corresponding enzymes, Pseudomonas putida mt-2 (XyIL) and Acinetobacter calcoaceticus (BenD), respectively. However, the amino arid sequences show 30% or less homology with those of Pseudomonas putida (BnzE), Pseudomonas putida Fl (TodD), Pseudomonas pseudoalcaligenes KF707 (BphB), and Pseudomonas sp. C18 (NahB). Therefore, the 4CBA-dihydrodiol dehdrogenase of strain S-47 belongs to the group I dehydrogenase involved in the degradation of mono-aryls with a carboxyl group.

DLTS기법에 의한 MOV소자의 교류과전경시 변화특성에 관한 연구 (A study on the degradation of the AC stressed MOV by using of the DLTS technique)

  • 이동희
    • E2M - 전기 전자와 첨단 소재
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    • 제9권7호
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    • pp.719-726
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    • 1996
  • DLTS measurements were performed to study the annealing induced changes of the trap centers in MOV and to shed more light on the stability mechanism of the MOV. Two electron traps, Ec-0.26[eV] and Ec-(O.2-0.3)[eV], were observed in the unannealed samples in large quantities(7-9 X 1014[CM 3]), whereas the three electron traps Ec-0.17 [eV], Ec-0.26[eV] and Ec-(O.2-0.3)[eV] were observed far less in the annealed samples. The minima in the Ec-0.26[eV] trap density, coupled with the presented results that unannealed devices are unstable whereas 600.deg. C annealed devices are most stable, suggests that the instability of the MOV under long term electrical stressing is related to the Ec-0.26[eV] trap. This results support that the ion migration model for the device instability where the Ec-0.26[eV] defects may be the interstitial zinc or the migrating ions. The interstitial zinc originated as a result of the nonstoichiometric nature of ZnO might cause the degradation of the I-V characteristics of the MOV with long term electrical stressing.

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Ecological health assessments using multiple parameters of fish blood tissues to community along with water chemistry in urban streams

  • Kang, Han-il;Choi, Ji-Woong;Hwang, Seock-Yeon;An, Kwang-Guk
    • Journal of Ecology and Environment
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    • 제38권3호
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    • pp.307-318
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    • 2015
  • The objectives of this study were to identify multi-level stressors from blood biomarkers to community-level bioindicators and diagnose the stream ecosystem health in polluted streams. Blood chemistry such as total protein ($T_{Pro}$), blood urea nitrogen ($B_{UN}$), total cholesterol ($T_{Cho}$) and $A_{lb}$umin ($A_{lb}$) were analyzed from sentinel fish tissues; the functions of kidney, gill and liver were significantly decreased in the impacted zone ($I_z$), compared to the control zone ($C_z$). Histopathological analysis showed that fish liver tissues were normal in the $C_z$. Fish liver tissues in the $I_z$, however, showed large cell necrosis and degeneration and also had moderate lobular inflammation and inflammatory cell infiltration of lymphocytic histocytes. Species biotic index (SBI) at species level and stream health assessment (SHA) at community level indicated that chemical impacts were evident in the $I_z$ (ecological health; poor - very poor), and this was matched with the blood tissue analysis and histopathological analysis. The impairments of the streams were supported by water chemistry analysis (nitrogen, phosphorus). Tolerance guild analysis and trophic guild analysis of fish were showed significant differences (P < 0.01) between $C_z$ and $I_z$. Overall, multiple parameter analysis from biomarker level (blood tissues) to bioindicator level (community health) showed significantly greater impacts in the $I_z$ than $C_z$. This approach may be effective as a monitoring tool in identifying the multilateral and forthcoming problems related to chemical pollution and habitat degradation of stream ecosystems.

Semiconductor coupled solar photo-Fenton's treatment of dyes and textile effluent

  • Raji, Jeevitha R.;Palanivelu, Kandasamy
    • Advances in environmental research
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    • 제5권1호
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    • pp.61-77
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    • 2016
  • $NanoTiO_2$ was synthesized by ultrasonication assisted sol-gel process and subjected to iron doping and carbon-iron codoping. The synthesized catalysts were characterized by XRD, HR-SEM, EDX, UV-Vis absorption spectroscopy and BET specific surface area analysis. The average crystallite size of pure $TiO_2$ was in the range of 30 - 33 nm, and that of Fe-$TiO_2$ and C-Fe $TiO_2$ was in the range of 7 - 13 nm respectively. The specific surface area of the iron doped and carbon-iron codoped nanoparticles was around $105m^2/g$ and $91m^2/g$ respectively. The coupled semiconductor photo-Fenton's activity of the synthesized catalysts was evaluated by the degradation of a cationic dye (C.I. Basic blue 9) and an anionic dye (C.I. Acid orange 52) with concurrent investigation on the operating variables such as pH, catalyst dosage, oxidant concentration and initial pollutant concentration. The most efficient C-Fe codoped catalyst was found to effectively destruct synthetic dyes and potentially treat real textile effluent achieving 93.4% of COD removal under minimal solar intensity (35-40 kiloLUX). This reveals the practical applicability of the process for the treatment of real wastewater in both high and low insolation regimes.

I/Q 불균형이 고려된 DVB-S2 시스템의 심벌 오류 확률 (Symbol Error Probability of DVB-S2 System with I/Q Unbalances)

  • 임인철;원승찬;윤동원;박상규
    • 한국통신학회논문지
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    • 제32권9C호
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    • pp.810-819
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    • 2007
  • 90도 위상 천이기나 필터와 같은 불완전한 시스템 요소에 의해 생성되는 I(inphase)축과 Q(quadrature)축 간의 불균형은 피할 수 없는 물리적 현상으로 동기 변/복조 시스템(coherent modulation/demodulation system) 구현에 있어서 성능 열화를 유발한다. 본 논문에서는 AWGN (additive white Gaussian noise) 채널 환경에서 수신기의 I/Q 불균형이 존재하는 경우, DVB(digital video broadcasting)-S2 시스템에서 사용되는 변조방식에 대한 정확하고 일반화된 심벌 오류 확률을 2차원 결합 가우시안 Q-함수(two-dimensional joint Gaussian Q-function)의 선형 결합(linear combination)의 형태로 유도한다.

Endo- 및 Exo-Inulinase를 이용한 Inulin 가수분해 (Hydrolysis of Inulin by Endo- and Exo-Inulinase)

  • 박선규;최용진
    • 한국미생물·생명공학회지
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    • 제19권1호
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    • pp.52-56
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    • 1991
  • 부분 정제한 Pseudomonas spp. Endo-inulinase와 Bacillus spp. Exo-inulinase를 13:1의 비율로 혼합 사용하여 inulin을 가수분해한 결과, 단독으로 사용했을 때에 비해 약 1.6배의 현저한 가수분해율의 상승효과를 얻었다. 혼합효소는 반응온도 $55^{\circ}C$, 반응액의 pH 6.0 및 각 0.5mM 농도와 $Mn^{2+}$$CO^{2+}$이 존재하는 반응조건하에서 가장 높은 가수분해율을 보였으며 이때 최종 가수분해 산물인 fructose의 생산량은 84를 나타내었다.

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MOS 소자의 대체 게이트 산화막으로써 $HfO_{2}/HfSi_{x}O_{y}$ 의 구조 및 전기적 특성 분석 (Structural and electrical characterizations of $HfO_{2}/HfSi_{x}O_{y}$ as alternative gate dielectrics in MOS devices)

  • 강혁수;노용한
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2001년도 하계학술대회 논문집
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    • pp.45-49
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    • 2001
  • We have investigated physical and electrical properties of the Hf $O_2$/HfS $i_{x}$/ $O_{y}$ thin film for alternative gate dielectrics in the metal-oxide-semiconductor device. The oxidation of Hf deposited directly on the Si substrate results in the H $f_{x}$/ $O_{y}$ interfacial layer and the high-k Hf $O_2$film simultaneously. Interestingly, the post-oxidation N2 annealing of the H102/H1Si70y thin films reduces(increases) the thickness of an amorphous HfS $i_{x}$/ $O_{y}$ layer(Hf $O_2$ layer). This phenomenon causes the increase of the effective dielectric constant, while maintaining the excellent interfacial properties. The hysteresis window in C-V curves and the midgap interface state density( $D_{itm}$) of Hf $O_2$/HfS $i_{x}$/ $O_{y}$ thin films less than 10 mV and ~3$\times$10$^{11}$ c $m^{-2}$ -eV without post-metallization annealing, respectively. The leakage current was also low (1$\times$10-s A/c $m^2$ at $V_{g}$ = +2 V). It is believed that these excellent results were obtained due to existence of the amorphous HfS $i_{x}$/ $O_{y}$ buffer layer. We also investigated the charge trapping characteristics using Fowler-Nordheim electron injection: We found that the degradation of Hf $O_2$/HfS $i_{x}$/ $O_{y}$ gate oxides is more severe when electrons were injected from the gate electrode.e electrode.e.e electrode.e.

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