• 생약학회지
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• 제52권2호
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• pp.112-117
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• 2021

Helianthus annuus L. has been reported with various pharmacological activities such as antibacterial, antidiabetic, and antioxidant effects. According to recent studies, H. annuus L. is known to contain components such as phenolic compounds, flavonoids, alkaloids, sesquiterpenoids, and lignans. The seeds of H. annuus L. have been reported to contain chlorogenic acid and di-O-caffeoylquinic acid as major components. However, studies on the main components and content of leaves of H. annuus L. are still incomplete. Therefore, in this study, the contents of four major components of H. annuus L. were evaluated by simultaneous quantitative analysis with high performance liquid chromatography (HPLC)-diode array detector (DAD). The isolated four compounds Caffeoylquinic acid(CQA), 3,4-dicaffeoylquinic acid(3,4-DCQA), 3,5-dicaffeoylquinic acid(3,5-DCQA) and 4,5-dicaffeoylquinic acid(4,5-DCQA) were shown in a large linearity with a correlation coefficient (R²) of 0.99. In addition, as a result of intra-inter day analysis of four major compounds by the analysis method of this study, the accuracy of 88.46% or more and less than 112.85% and excellent precision of less than 3% were shown, the content analysis showed CQA (0.383±0.018 mg/g), 3,4-DCQA (0.282±0.017 mg/g), 3,5-DCQA (1.109±0.068 mg/g), and 4,5-DCQA (0.673±0.020 mg/g).

• 공업화학
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• 제19권5호
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• pp.477-483
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• 2008

구연산법을 이용하여 $La_{0.7}Sr_{0.3}Co_{0.2}Fe_{0.8}O_{3-{\delta}$ 산화물을 합성하였으며, 합성된 분말은 압축 성형 후 $1300^{\circ}C$에서 소결하여 치밀한 페롭스카이트 분리막을 제조하였다. 구연산법으로 제조한 $La_{0.7}Sr_{0.3}Co_{0.2}Fe_{0.8}O_{3-{\delta}$의 전구물질은 TGA와 XRD로 분석하였다. $260{\sim}410^{\circ}C$ 온도 영역에서 전구물질의 금속-구연산 복합체가 분해되며 페롭스카이트 산화물이 얻어지나 XRD 분석결과 $900^{\circ}C$ 이하에서는 $SrCO_3$가 불순물로 존재하였다. 분리막의 전기전도도는 온도가 증가함에 따라 증가하다. 결정격자의 산소 손실로 인해 공기분위기에서는 $700^{\circ}C$ ($Po_2=0.2atm$)부터, 헬륨분위기에서는 $600^{\circ}C$ ($Po_2=0.01atm$) 부터 각각 감소하였다. 산소투과량은 온도가 증가할수록 증가하였고, 두께 1.6 mm의 $La_{0.7}Sr_{0.3}Co_{0.2}Fe_{0.8}O_{3-{\delta}$ 분리막은 $950^{\circ}C$에서 $0.31cm^3/cm^2{\cdot}min$의 최대 투과도를 보였다. 산소투과에 대한 활성화 에너지는 $750{\sim}950^{\circ}C$ 온도 영역에서 88.4 kJ/mol이었다. 40 h의 투과실험 후에 분리막의 페롭스카이트 결정 구조는 변하지 않았으며 0.3 mol Sr doping 시 2차상이 생성되지 않고 안정하였다.

• 공업화학
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• 제7권5호
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• pp.946-953
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• 1996

동시조사법에 의한 아크릴산의 폴리프로필렌 부직포에 대한 그라프트 반응에서 조사량, 조사선량률, 금속염 및 용매가 그라프트 반응에 미치는 영향을 조사하였다. 같은 조사선량에서는 선량률이 낮을수록 높은 그라프트율을 나타냈다. 한편 조사선량률을 달리하여 일정 시간 동안 그라프트 반응시키면 조사선량률이 높을수록 그라프트율은 높았으며, 첨가한 금속염의 종류에 관계없이 그라프트 속도는 조사선량률의 0.74 차수에 비례하였다. 사용한 금속염 중에서 $FeSO_4{\cdot}7H_2O$가 가장 우수하였으며, 첨가제의 함량이 증가하면 할수록 그라프트율은 감소하였지만 적정농도이하에서는 그라프트용액이 고화되어 그라프트 반응시킬 수 없었다.

• Korean Journal of Acupuncture
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• 제28권3호
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• pp.43-51
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• 2011

Objectives : In this study, we investigated the effect of Fructus Ligustri Lucidi $H_2O$ fraction (FLLW) on cell proliferation, and the phosphorylation of ERKs and Akt in human dermal fibroblast neonatal (HDFn). Methods : After treatment of HDFn with FLLW, MTT assay was performed to quantitatively determine cellular viability. The ERK and Akt pathways were analyzed in vitro by Western blot in a HDFn. HDFn proliferation after FLLW and minoxidil treatment in the absence or presence of PD98059, a MEK inhibitor, LY294002, and a PI3K inhibitor, was examined by Western blot or MTT assay. Results : FLLW increased cell proliferation in a dose-dependent manner and minoxidil used as positive control also induced cell proliferation in HDFn. FLLW increased the phosphorylation of ERK and Akt. In addition, minoxidil, too, induced the phosphorylation of ERK and Akt in HDFn. PD98059 and LY294002 significantly attenuated FLLW-inducible p-ERK and p-Akt expression and proliferation in cultured HDFn. Conclusions : Our results suggest that FLLW stimulates the growth of fibroblast cells through ERK and Akt pathways. Therefore, FLLW is a potential agent for the inducer of fibroblast growth.

• 대한환경공학회지
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• 제27권12호
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• pp.1327-1331
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• 2005

본 연구에서는 해안연약지반의 현장토(준설점토)를 차수층에 이용하기 위해 첨가되는 고화재에 의한 수화열을 저감하기 위한 방안을 모색하고자 산업부산물로 년간 다량 배출되는 고로슬래그를 이용하였다. 실험결과, 고로슬래그를 첨가한 준설혼합토(준설점토 95% w+시멘트 5% w)의 경우 고화재에 의한 초기수화반응열을 저감하는 것으로 나타났는데 이는 불투수성인 aluminosilicate 피막이 고로슬래그의 표면을 코팅하였기 때문으로 판단된다. 따라서 고화재의 수화반응에 따른 수화열에 의해 발생될 수 있는 매립지 차수층의 미세균열을 산업부산물인 고로슬래그가 저감해 줄 수 있을 것으로 판단된다.

• 한국세라믹학회지
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• 제42권8호
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• pp.582-587
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• 2005

AlN-BN ceramic composites were fabricated and their mechanical properties were investigated. The relative density of hot-pressed composites decreased with increasing BN content, but over $99\%$ could be obtained with 30 $vol\%$ BN in AlN. YAG was formed in the composites and monolithic AlN as a second phase by the reaction between $Y_2O_3$, added as sintering aid, and $Al_2O_3$. As expected, Vickers hardness and Young's modulus decreased with increasing BN content. The three-point flexural strength also showed similar behavior decreasing from 500 MPa of monolith down to 250 MPa by the addition 30 $vol\%$ BN. However, interestingly, the standard deviation of the strength values decreased significantly as BN was added to AlN. As a result, the Weibull modulus of the AlN-30 $vol\% BN composite was 21.3, which was extremely high. Fractography and crack path studies revealed that BN platelets induced grain pull-out and crack bridging in a bigger scale during crack propagation. Consequently, fracture toughness increased as more BN was added, reaching 4.5 $MPa\sqrt{m}$ at 40 $vol\%$ BN.

• 한국식품조리과학회지
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• 제28권2호
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• pp.175-182
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• 2012

In this study, antibacterial activity and cytotoxicity of the extracts from pericarp and seed of $Vitis$ $coignetiea$, which were extracted with 0.1% HCl-60% ethanol, were analyzed. The antibacterial activity of the extracts was determined by paper disc diffusion method against food spoilage and food-borne pathogens. The pericarp extract showed high antibacterial activity against $Bacillus$ $cereus$, $Escherichia$ $coli$ O157:H7, and $Pseudomonas$ $aeruginosa$, and the seed extract represented the high antibacterial activity against $B.$ $cereus$, $E.$ $coli$ O157:H7, and $Staphylococcus$ $aureus$. The cytotoxicity of the $Vitis$ $coignetiea$ extract against human cancer cells was determined using the MTT assay and SRB assay. The pericarp extract represented strong growth-inhibition activity against G361 and Hep3B cells and the seed extract greatly inhibited the growth of HeLa and G361 cells in the MTT assay. In addition, the pericarp extract displayed a high inhibition activity against the growth of AGS cells and the seed extract greatly inhibited the growth of HeLa, Hep3B, and MCF7 cells in the SRB assay. Especially, the cytotoxicities of the seed extract against HeLa were significantly higher than those of the extract against other cancer cells at all test concentrations. This study demonstrates that the extract from pericarp and seed of $Vitis$ $coignetiea$ possess high antibacterial activity and cytotoxicity.

• 한국환경농학회지
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• 제17권1호
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• pp.11-15
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• 1998

이 연구는 담수토양 조건에서 토양에 처리된 carbofuran이 시간의 경과에 따라 분해될 때 토양에 존재하는 미생물적 요인이 carbofuran의 분해에 기여하는 정도를 분석하고, 토양내 유기탄소의 양이 비교적 풍부할 때 토양에 존재하는 황산염 성분이 carbofuran의 분해 속도에 미치는 영향을 평가하기 위한 것으로서, 1996년 3월 하순 경기도 시흥시의 논에서 채취한 토양을 대조토양으로 하여 이 대조토양에 cellulose와 황산염의 첨가와 멸균 처리를 하여 대조토양, 멸균토양, 10%의 cellulose 첨가 토양, 10% cellulose와 1%의 황산제일철($FeSO_4 \;{\cdot}\;7H_2O$) 동시 첨가 토양, 그리고 10% cellulose와 1% 황산마그네슘($MgSO_4 \;{\cdot}\;7H_2O$) 동시 첨가 토양 등의 다섯 종류의 토양시료를 만들었다.이들 토양시료에 총 농도 50ppm가 되도록 carbofuran을 첨가하여 $25^{\circ}C$에서 4주간 배양하면서 carbofuran의 토양내 잔류량을 측정하였다.멸균토양의 carbofuran의 잔류량은 대조토양에 비해 매우많았는데(p<0.05), 4주 동안 배양시 대조토양에 비해 잔류량이 약 18% 정도 많았다. 토양내 carbofuran의 잔류량을 배양 4주 동안 측정한 결과, 10%의 cellulose와 황산마그네슘을 동시에 첨가한 토양에서 carbofuran의 잔류량이 대조토양과 10% cellulose만을 첨가한 토양이나 10% cellulose와 황산재일철 동시 첨가 토양에 비해많아서 (p<0.01), 황산염과 마그네슘 함량이 일정 농도 이상으로 높은 토양에서 carbofuran의 분해가 지연되는 결과를 나타내었다.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2018년도 춘계학술대회 논문집
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• pp.23-23
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• 2018

In this study, new titanium alloys were prepared by adding elements such as tantalum (Ta), zirconium (Zr) and the like to complement the biological, chemical and mechanical properties of titanium alloys. The Ti-40Ta-xZr ternary alloy was formed on the basis of Ti-40Ta alloy with the contents of Zr in the contents of 0, 3, 7 and 15 wt. %. Plasma electrolytic oxidation (PEO), which combines high-voltage sparks and electrochemical oxidation, is a novel method to form ceramic coatings on light metals such as Ti and its alloys. These oxide film produced by the electrochemical surface treatment is a thick and uniform porous form. It is also composed of hydroxyapatite and calcium phosphate-based phases, so it has the characteristics of bone inorganic, non-toxic and very high bioactivity and biocompatibility. Ti-40Ta-xZr alloys were homogenized in an Ar atmosphere at $1050^{\circ}C$ for 1 hour and then quenched in ice water. The electrochemical oxide film was applied by using a power supply of 280 V for 3 minutes in 0.15 M calcium acetate monohydrate ($Ca(CH_3COO)_2{\cdot}H_2O$) and 0.02 M calcium glycerophosphate ($C_3H_7CaO_6P$) electrolyte. A small amount of 0.0075M zinc acetate and magnesium acetate were added to the electrolyte to enhance the bioactivity. The mechanical properties of the coated surface of Ti-40Ta-xZr alloys were evaluated by Vickers hardness, roughness test, and elastic modulus using nano-indentation, and the surface wettability was evaluated by measuring the contact angle of the coated surface. In addition, cell activation and differentiation were examined by cell culture of HEK 293 (Human embryonic kidney 293) cell proliferation. Surface properties of the alloys were analyzed by scanning electron microscopy(FE-SEM), EDS, and X-ray diffraction analysis (XRD).

• 한방재활의학과학회지
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• 제33권3호
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• pp.17-32
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• 2023

Objectives The purpose of this study was to investigate the antioxidant, anti-inflammatory and cartilage regeneration effects of Euiiin-tang water extract (EIIT) in the treatment of monosodium iodoacetate (MIA)-induced osteoarthritis in rats. Methods Animal models were divided into five groups. The normal group didn't do any treatments causing osteoarthritis. The control group was orally administerd distilled water instead of the drug, the positive control group used indomethacin 5 mg/kg, the EIIT 100 group used EIIT 100 mg/kg and the EIIT 200 group used EIIT 200 mg/kg, and seven rats were placed per group. We administered drug to rats for 2 weeks and analyzed oxidative stress-related proteins in joint tissue. Inflammation mediators and inflammatory cytokines induced by the activity of inflammation-related proteins were analyzed. In addition, the expression of anti-inflammatory cytokines and collagen-related factors were analyzed, and H&E staining and Safranin-O staining were performed to see the effect on histopathological changes. Results 1) Oxidative stress-related proteins were significantly reduced. 2) Inflammationrelated proteins, inflammatory mediators and inflammatory cytokines were significantly reduced. 3) Anti-inflammatory cytokines were significantly increased. 4) Collagen proteolysis factors significantly decreased, and collagen degradation inhibitory factor was significantly increased. 5) EIIT administration significantly reduced cartilage degeneration and deformation in H&E staining, and reduced proteoglycan destruction in Safranin-O staining. Conclusions From the above experimental results, it judges that Euiiin-tang has antioxidant, anti-inflammatory, and cartilage regeneration effects on osteoarthritis in rats induced by MIA.