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Effects of pH and Natural Polysaccharide Gums on the Foam Stability of Soy Protein Isolate (pH와 천연 다당류 고무질이 분리대두단백질의 거품안정성에 미치는 영향)

  • Yang, Seung-Taek;Kim, Mi-Sook;Park, Chun-Og
    • Korean Journal of Food Science and Technology
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    • v.24 no.5
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    • pp.482-491
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    • 1992
  • Experiments were conducted to elucidate the effects of pH change and sodium alginate, gum karaya and gum arabic on the foaming properties of soy protein isolate (SPI). The surface tensions of SPI solution (5%) adding to gums at pH 4.0 and 5.0, near the isoelectric point (pH 4.5) were higher than those at pH 7.0 and 8.0. Specific viscosity of the solutions adding to gums (0.1, 0.2 and 0.3%) at pH 7.0 and 8.0 were $3.6{\sim}51.8$ and $4.0{\sim}51.2$, respectively. In cases of addition of sodium alginates specific viscosity were predominantly increased, while those of gum arabic did not almost increase. Addition of gums reduced the overrun, but it stabilized the foams, especially those of sodium alginates (0.2%) increased the foam stability at pH 5.0, 7.0 and 8.0 by 57, 413 and 315%, respectively. Foaming ability, foam stability and heat stability of the foam were significantly (p<0.05) affected by changing pH. There was a tendency to increase the foaming ability according to the decrease of surface tension. Specific viscosity was also important in foam stability and heat stability.

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Gastric Epithelial Cell Proliferation and Apoptosis in Children with Helicobacter pylori Infection (소아 Helicobacter pylori 감염에서 위 상피세포의 증식과 세포사)

  • Jung, Ji-Ah;Zhe, Jin;Han, Woon-Sup;Seo, Jeong-Wan
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.5 no.1
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    • pp.1-10
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    • 2002
  • Purpose; Dysregulation of gastric epithelial cell proliferation and apoptosis are important in development of ulcer, atrophy and neoplasia in Helicobacter pylori (H. pylori) infection. The aim of this study was to investigate the effect of infection of H. pylori on gastric epithelial cell proliferation and apoptosis in children. Methods: Histological grading by updated Sydney system, PCNA immunostaining and TUNEL method were performed in H. pylori positive (N=58) and negative (N=40) gastric biopsy specimens. Results: In H. pylori positive children, there were significantly higher grade of polymorphonuclear neutrophil activity (P=0.000), chronic inflammation (P=0.000), epithelial damage (P=0.000) and lymphoid follicles (P=0.000) than in H. pylori negative children. Intestinal metaplasia was not seen in H. pylori positive children. PCNA index was significantly different between H. pylori positive children ($67.8{\pm}18.13$) and H. pylori negative children ($54.8{\pm}14.46$, P=0.000). There was positive correlation between PCNA index and H. pylori density (r=0.277, P=0.007), polymorphonuclear neutrophil activity (r=0.280, P=0.007) and chronic inflammation (r=0.284, P=0.006). Apoptosis index of H. pylori positive children ($0.44{\pm}0.447$) was significantly higher than of H. pylori negative children ($0.14{\pm}0.196$, P=0.000). There was positive correlation between apoptosis index and H. pylori density (r=0.472, P=0.000), polymorphonuclear neutrophil activity (r=0.370, P=0.001) and chronic inflammation (r=0.483, P=0.000). There was positive correlation between PCNA index and apoptosis index (r=0.353, P=0.003). Conclusion: The PCNA and apoptosis index in H. pylori positive children were significantly higher than in H. pylori negative children. This study suggested that gastric epithelial cell proliferation and apoptosis are important to pathogenesis of H. pylori infection in children.

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The Growth Response of Balloon Flower (Platycodon grandiflorum A. DC.) Plantlets In Vitro as Affected by Air Exchanges and Light Intensity (배양용기 내 환기와 광도에 따른 도라지(Platycodon grandiflorum A. DC.) 기내 배양묘의 생장반응)

  • Choi So-Ra;Kim Myung-Jun;Eun Jong-Seon;Ahn Min-Sil;Lim Hoi-Chun;Ryu Jeong
    • Journal of Plant Biotechnology
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    • v.32 no.1
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    • pp.23-29
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    • 2005
  • Shoots of balloon flower (Platycodon grandiflorum A. DC.) derived from in vitro germinated seeds were cultured on MS medium containing $0.1\;\cal{mg/L}$ NAA under various photosynthetic photon flux (PPF) 33, 66, and $99\;{\mu}mol\;m^{-2}s^{-1}$ with or without membrane filter. Number of air exchanges per hour (NAEH) of the culture vessel with membrane filter on the lid was $4.9 h^{-1}$ and that without membrane filter was $0.1 h^{-1}$ Plantlets grown in $4.9 h^{-1}$ NAEH showed greater growth than in $0.1 h^{-1}$ NAEH. According to increase of PPF, plantlets growth decreased in $0.1 h^{-1}$ NAEH while it increased in $4.9 h^{-1}$ NAEH. At the same PPF, fresh weight and sugar content in plantlets in $4.9 h^{-1}$ NAEH were above 1.9, 2.0 times higher than those in $0.1 h^{-1}$ NAEH, respectively. Also they were enhanced in $4.9 h^{-1}$ NAEH by increase of PPF whereas no significance in $0.1 h^{-1}$ NAEH. The percentage of water content of plantlets in $4.9 h^{-1}$ NAEH was $4.2\~5.5\%$ lower than those in $0.1 h^{-1}$ and no difference in PPF. The content of total chlorophyll in plantlets in $4.9 h^{-1}$ NAEH was higher $0.27\~0.79\;\cal{mg/g}$ F.W. than that in $0.1 h^{-1}$ NAEH. By increase of PPF, it was decreased in $0.1 h^{-1}$ NAEH while had no significant difference in $4.9 h^{-1}$ NAEH. Guard and subsidiary cells of leaves in $4.9 h^{-1}$ NAEH were more developed than in $0.1 h^{-1}$ NAEH. Especially, in $99\;{\mu}mol\;m^{-2}s^{-1}$ leaves in $0.1 h^{-1}$ NAEH had undeveloped subsidiary cells and wide open stomata whereas those in $4.9 h^{-1}$ NAEH had well-developed subsidiary cells.

Effect of pHs on Morphological and Cultural Characteristics of Alkalophilic Coryneform Bacteria TU-19 (호알칼리성 Coryneform Bacteria TU-19의 형태적, 배양적 특성에 미치는 pH효과)

  • Choi, Myoung-Chul;Yang, Jae-Sub;Hwang, Cher-Won;Kang, Sun-Chul
    • Applied Biological Chemistry
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    • v.41 no.5
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    • pp.337-341
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    • 1998
  • The morphological and cultural characteristics of alkalophilic Coryneform bacteria TU-19 were investigated at various pHs. This bacterium showed normal growth pattern at $pH\;9.0{\sim}10.0$, but the cell growth was completely inhibited at extreme pH (12.0 or more). Interestingly, at pH 8.0 the morphology of the bacterial cells seems to form convoluted filaments during the exponential growth phase while at pH 10.0, the optimal pH for the growth of this organism, the bacteria grew with variable paired or single forms, and straight rods during growth stages. Growing in alkaline media $(pH\;9.0{\sim}11.0)$, it adjusted the pH of the culture media to around pH 8.5 by itself.

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Effects of sires with different weight gain potentials and varying planes of nutrition on growth of growing-finishing pigs

  • Ha, Duck-Min;Jung, Dae-Yun;Park, Man Jong;Park, Byung-Chul;Lee, C. Young
    • Journal of Animal Science and Technology
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    • v.56 no.6
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    • pp.22.1-22.7
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    • 2014
  • The present study was performed to investigate the effects of two groups of sires with 'medium' and 'high' weight gain potentials (M-sires and H-sires, respectively) on growth of their progenies on varying planes of nutrition during the growing-finishing period. The ADG of the M-sires' progeny was greater (P < 0.05) than that of the H-sires' progeny (0.51 vs. 0.47 kg) during a 26- to 29-d early grower phase beginning from 55 d of age, but the opposite was true (0.66 vs. 0.72 kg) during the latter grower phase. Overall grower-phase ADG was greatest on the high plane of nutrition (H plane) followed by the medium (M) and low (L) planes (0.65, 0.61, and 0.51 kg, respectively; P < 0.05) in the M-sires' progeny, whereas in the H-sires' progeny, ADG was greater on the H and M planes vs. L plane (0.63, 0.62, and 0.54 kg, respectively). The ADG of pigs on the M or H plane during the grower phase and switched to the H plane thereafter (M-to-H or H-to-H planes) was greater than that of pigs on the L-to-L planes (0.99 vs. 0.78 kg) during the early finisher phase in the M-sires' progeny (P < 0.01). However, in the H-sires' progeny, ADG of pigs on the L-to-L planes did not differ from that of pigs on the M-to-M or H-to-M planes (0.94 vs. 0.96 kg). Results suggest that the H-to-H or H-to-M planes and M-to-M or M-to-L planes are optimal for maximal growth of the M- and H-sires' progenies, respectively.

The Anti-emetic Effect of Banhasasim-tang Intravenous Herbal Acupuncture in MTX-induced Rat-Pica Model (반하사심탕 혈맥약침이 흰쥐의 CINV(Chemotherapy-Induced Nausea and Vomiting)에 미치는 영향)

  • Cho, Young-Kwon;Lee, Chan;Lee, Hyun-Jin;Yim, Yun-Kyoung
    • The Journal of Korean Medicine
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    • v.38 no.1
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    • pp.34-45
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    • 2017
  • Objectives: This study aimed to investigate the effect of banhasasim-tang intravenous herbal acupuncture (BST-IVHA) on emesis induced by chemotherapy in rats. Methods: This study used methotrexate(MTX)-induced Rat-Pica model. The rats were randomly allocated into seven groups; normal group, two saline groups, four Banhasasim-tang(BST) groups (groups treated with BST-IVHA). All the experimental animals except those in the normal group were injected with MTX. Those in the pre-treatment groups were treated with saline injection (saline group) or BST-IVHA (BST group) before MTX injection. Those in the post-treatment groups were treated with saline injection or BST-IVHA after MTX injection. Two different dosages of BST-IVHA solution (low dose; BST-1 group, high dose; BST-2 group) were used. The changes in body weight, food intake, and kaolin consumption at 24h, 48h, and 60h were monitored and analyzed. Results: 1. No significant change was found in body weight. 2. The food intake at 48h was increased significantly in the BST-1 pre-treatment group($19.89{\pm}0.01g$) compared to the pre-saline group($18.68{\pm}0.26g$). 3. The kaolin consumption was significantly decreased in the BST-1 pre-treatment group at 24h($0.24{\pm}0.02g$) and 60h($0.36{\pm}0.14g$), in the BST-2 pre-treatment group at 48h($0.02{\pm}0.01g$) and 60h($0.80{\pm}0.31g$) compared to the pre-saline group($24h:0.81{\pm}0.37g$, $48h:0.76{\pm}0.43g$, $60h:1.56{\pm}0.03g$). The kaolin consumption was also significantly decreased in the in the BST-1 post-treatment group at 24h($0.05{\pm}0.02g$), 48h($0.64{\pm}0.06g$) and 60h($0.14{\pm}0.05g$), in the BST-2 post-treatment group at 48h($0.01{\pm}0.01g$) and 60h($0.01{\pm}0.01g$) compared to the post-saline group($24h:0.51{\pm}0.4g$, $48h:3.58{\pm}0.33g$, $60h:2.5{\pm}0.2g$). Conclusions: BST-IVHA showed an anti-emetic effect in MTX-induced rat-pica model. This result suggests that BST-IVHA could be an effective treatment for chemotherapy-induced emesis.

Effects of Green Tea-Soybean Paste on Weights and Serum Lipid Profiles in Rats Fed High Fat Diet (녹차 된장이 고지방식이를 급여한 흰쥐의 체중 및 혈청 지질성분에 미치는 영향)

  • Park, Jin-Hee;Ha, Ae-Wha;Cho, Jung-Soon
    • Korean Journal of Food Science and Technology
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    • v.37 no.5
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    • pp.806-811
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    • 2005
  • Effects of green tea-soybean paste on weight-changes and serum lipid profiles in rats fed high fat diet were investigated. Sprague-Barley male rats divided into five groups were fed five different diets for 10 weeks: B group, basal diet H group, high fat-diet containing 0.5% cholesterol+15% lard H-S group, high fat diet containing soybean paste H-0.6GS group, high fat diet containing 0.6% green tea-soybean paste H-4GS group, high fat diet containing 4% green tea-soybean paste. H group showed highest weight gain (p<0.05). Addition of green-tea soybean paste to high-fat diet significantly decreased weight gain, H-4GS group showing highest weight loss (p<0.05). No significant differences in food efficiency ratio and total food intake were found among groups. Liver weight of H group was highest. Addition of green tea-soybean paste to high fat diet significantly reduced liver weight (p<0.05, Table 2). Concentrations of glucose, uric acid, bilirubin, and total protein were similar among all group. Mean GPT values of H-4GS group were significantly different from these of H- and H-S groups (p<0.05). H-S, H-0.6GS, and H-4GS groups had significantly lower triglyceride and total cholesterol, and higher HDL than H-group (p<0,05). H-4GS group had significantly lower serum triglyceride than H-0.6GS group (p<0.05).

pH Variance Model Depending on Phosphate Ion Form (인산염 이온 형태에 의한 pH 변량 모형)

  • Soh, Jae-Woo;Soh, Soon-Yil;Nam, Sang-Yong
    • Horticultural Science & Technology
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    • v.33 no.6
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    • pp.854-859
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    • 2015
  • This experiment was performed to develop a model for nutrition ion concentration and EC in regard to change in pH from 4.0 to 8.0 in nutrient solution. The pH changes according to the variation of $HPO_4{^{-2}}$ and $H_2PO_4{^-}$ in the nutrient solution while variation of EC increased from pH 4.0 to 5.0, stabilized from pH 5.0 to 7.0 and increased again from pH 7.0 to 8.0. For the variance of major elements in the nutrient solution, K, Ca, N and P increased while pH was higher, especially the variables for K and P were increased largely. On the other hand, variables of Mg and S were stable. Based on analysis of the ion balance model of nutrient solution, the cation increased than anion over rising the variation of pH while balance point of ion moved from a-side to d-side. In addition, the imbalance increased while it moved away from the EC centerline as variance of pH increased. It was larger than effect of EC variance to correction values of equivalence ratios of K and Ca about variation of $HPO_4{^{-2}}$ and $H_2PO_4{^-}$ while as variance of pH increased, K decreased but Ca increased. These showed the result that variance of pH about correction values of equivalence ratios of K and Ca gave a second-degree polynomial model rating of 0.97. Through this research, it was identified the pH variable model about variance among pH, ion and EC according to gradient of phosphate.

Effects of Simulated Acid Rain on Mineral Nutrient Movement in Soil (인공산성비 처리가 토양의 무기양분 이동에 미치는 영향)

  • Ryu, Kwan-Shig
    • Korean Journal of Environmental Agriculture
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    • v.17 no.4
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    • pp.362-367
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    • 1998
  • To investigate the effects of simulated acid rain(SAR) on the downward movement of mineral nutrients, SARs of different pH were applied to the soil. SAR of pH 2.0 decreased the soil pH greatly, while SAR of pH 4.0 and 6.0 did not change the soil pH to compare to that of SAR of pH 2.0. Decrease in soil pH was in the order of sandy loam > loam > clay loam. The amoumt of leached exchangeable and soluble bases from the soil due to the penetration of SAR was in the order of Ca >Mg > K. After application of 1200mm SAR of pH 2.0 in to the soil downward mean movements of the exchangeable and soluble bases was in the order of Mg > Ca > K in sandy loam and loam soil and Ca > Mg > K in clay loam soil. Downward movements of the those bases under pH 4.0 into the soil was in the order of Mg > K > Ca in sandy loam and clay loam, and K > Mg > Ca in loam soil. Available phosphorus moved slightly downward with increasing acidity of the SAR.

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The Effect of Protein Extraction pH on the Components of Sesame Protein Concentrates (단백질 추출 pH가 참깨 농축단백질의 성분에 미치는 영향)

  • 박정륭;김은정
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.4
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    • pp.613-618
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    • 1995
  • This study was carried out to investigate the effect of extracting pH on the components and color of seasame protein concentrate(SPC). The protein contents of SPC by extracted at pH 2.0, 7.0, 9.0 and 11.0 were 60.57, 67.72, 79.50 and 83.44%, respectively. Most of the phytates were removed in SPC extracted at pH 7.0, 9.0 and 11.0, but the phytate content of SPC extracted at pH 2.0 was about the same as that of defatted sesame flour. The highest decrease of phytate was found in SPC extracted at pH 11.0(94.80%). SPC extracted at pH 2.0 contained the highest amount of Ca, Mg, Fe and Zn than those in other SPC prepared, while highest amount of Cu was found in SPC extracted at pH 11.0. Sodium content was similar among all the SPC prepared. SPC extracted at pH 7.0 resulted in brighter clor, but SPC extracted at pH 11.0 showed a little darker in appearance.

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