• 한국정보과학회:학술대회논문집
• /
• 한국정보과학회 2005년도 가을 학술발표논문집 Vol.32 No.2 (1)
• /
• pp.247-249
• /
• 2005

최근 디지털 TV, DVD 플레이어, 홈시어터와 같은 디지털 A/V 기기들을 통합하여 멀티미디어 컨텐츠를 서비스 받는 홈 네트워크 기술에 대한 관심이 높아지고 있다. 이러한 환경에서 UpnP를 이용한 디지털 기기들의 상태를 모니터링하고, 사용자의 요청과 제어 명령을 전달하여, 멀티미디어 컨텐츠를 서비스 받을 수 있도록 하는 연구는 매우 중요하다. 그러나 UPnP는 TCP/IP 기반의 프로토콜을 사용하기 때문에 사용자 인터랙션에 의한 전송 지연 시간이 발생한다. 그러므로 본 논문에서는 셋탑 박스에 최적화된 UPnP AV 시스템을 구현하고 AV 컨텐츠의 효율적인 재생을 위한 UPnP프레임워크의 재생 기법을 제안한다. UPnP AV 시스템은 미디어 서버가 있고, 미디어 서버에 저장된 비디오/오디오 파일 등을 전송받아 재생하기 위한 셋탑 박스에는 컨트롤 포인트와 UPnP 미디어 렌더러의 통합 모델이 탑재된다. 이러한 기법은 다양한 기기를 제어하고 호환성을 제공하는 UPnP프레임워크가 될 수 있다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제21권12호
• /
• pp.1703-1709
• /
• 2008

As genetic markers, single nucleotide polymorphisms (SNP) are very appropriate for the development of genetic tests for economic traits in livestock. Several microsatellite markers have been identified as useful markers for the genetic improvement of Hanwoo. Among those markers, ILSTS035 was recently mapped at a similar position with four SNPs (AH1_11, AH1_9, 31465_446, and 12273_165) in a linkage map of EST-based SNP in BAT6. Among the four SNPs, two SNPs (31465_446 and 12273_165) were analyzed using BLAST at the NCBI web site. The sequences including the 12273_165 SNP were identified at the intron region within the LOC534614 gene on the gene sequence map (Bos taurus NCBI Map view, build 3.1). The LOC534614 gene represents a protein similar to myosin heavy chain, fat skeletal muscle, embryonic isoform 1 in the dog, and myosin_1 (Myosin heavy chain D) in Macaca mulatta. In cattle, the myosin heavy chain was associated with muscle development. The phenotypic data for growth and carcass traits in the 415 animals were analyzed by the mixed ANCOVA (analysis of covariance) linear model using PROC GLM module in SAS v9.1. By the genotyping of Hanwoo individuals (n = 415) to evaluate the association of SNP with growth and carcass traits, it was shown that the 12273_165 SNP region within LOC534614 may be a candidate marker for growth. The results of the statistical analyses suggested that the genotype of the 12273_165 SNP significantly affected birth weight, weight of the cattle at 24 months of age, average daily gain and carcass cold weight (p<0.05). Consequently, the 12273_165 SNP polymorphisms at the LOC534614 gene may be associated with growth in Hanwoo, and functional validation of polymorphisms in LOC534614 should be performed in the future.

• 한국미생물·생명공학회지
• /
• 제48권2호
• /
• pp.158-166
• /
• 2020

Paenibacillus woosongensis의 xylanase 유전자를 클로닝하고 그 염기서열을 결정하였다. Xylanase 유전자는 xyn10A로 명명되었으며, 481 아미노잔기로 구성된 단백질을 코드하는 1,446개 뉴클레오티드로 구성되었다. 추론된 아미노산 배열에 따르면 Xyn10A는 glycosyl hydrolase family 10 xylanase와 상동성이 높은 활성영역과 카르복실 말단에 탄수화물을 결합하는 것으로 추정되는 영역이 포함된 다영역 효소로 확인되었다. DEAE-Sepharose와 Phenyl-Separose 컬럼 크로마토그래피 과정을 통해 P. woosongensis xyn10A 유전자를 함유한 재조합 대장균의 균체 파쇄상등액으로부터 Xyn10A를 정제하였다. 정제된 Xyn10A의 아미노 말단 배열이 GIANGSKF로 결정되었으며 이는 SignalP5.0 server로 예측된 signal peptide의 다음 아미노산 배열과 정확하게 일치하였다. 정제된 Xyn10A는 33 kDa 크기의 절단된 단백질이며 균체내 분해에 의해 카르복시 말단에서 CBM이 제거된 것으로 판단된다. 정제된 효소는 최적 pH와 온도가 6.0과 55-60℃이며 oat spelt xylan에 대한 반응 동력학적 계수 V_max와 K_m이 298.8 U/mg과 2.47 mg/ml로 각각 나타났다. 효소는 birchwood xylan이나 oat spelt xylan보다 arabinoxylan에 대한 활성이 높았으며 para-nitrophenyl-β-xylopyranoside에 대해 낮은 활성을 보였다. Xyn10A의 활성은 Cu²⁺, Mn²⁺과 SDS에 의해서 크게 저해되었으며 K⁺, Ni²⁺과 Ca²⁺에 의해는 상당하게 증진되었다. 또한 이 효소는 xylobiose 보다 중합도가 큰 자일로올리고당을 분해하였으며, 자일로올리고당의 최종 가수분해 산물은 xylose와 xylobiose로 확인되었다.

• 한국광학회지
• /
• 제26권5호
• /
• pp.269-274
• /
• 2015

본 논문에서는 code V 시뮬레이터를 이용하여 광 HDMI 인터페이스에 적용하기 위한 집적형 2채널 TO 구조 광송신기의 광학계 설계를 하였다. 제안된 집적형 2채널 TO 구조 광송신기는 지름의 6.4 mm인 하나의 8 pin TO-CAN 패키지 안에 2개의 VCSEL을 평행하게 부착하고 그 위에 반구(hemisphere) 렌즈가 부착된 직육면체 모양($1mm(H){\times}2mm(W){\times}4mm(D)$)의 렌즈필터 블록 (lens filter block) 을 올려놓은 구조이다. 집적형 2채널 TO 구조 광송신기 제작 시 1060 nm/1270 nm와 1330 nm/1550 nm로 두 개 파장씩 묶어 TO-CAN 형태로 제작하므로, 이들 파장 조합에서 반구 렌즈의 지름은 0.6 mm, 볼 렌즈와 렌즈 필터 간격(L)은 파장 1060 nm/1270 nm 조합의 경우 1.7 mm, 파장 1330 nm/1550 nm 조합의 경우 2.0 mm일 때 최적의 결과를 얻을 수 있었다. 이때 렌즈필터 블록의 초점거리($f_0$)는 1060 nm, 1270 nm, 1330 nm, 1550 nm 파장에 대하여 0.351 mm, 0.354 mm, 0.355 mm, 0.359 mm이었으며 렌즈 필터 블록과 볼 렌즈를 통과한 빛의 초점 위치(F)는 파장 1060 nm/1270 nm 조합의 경우 0.62 mm, 파장 1330 nm/1550 nm 조합의 경우 0.60 mm ~ 0.66 mm이었다.

• Journal of Microbiology and Biotechnology
• /
• 제20권3호
• /
• pp.518-524
• /
• 2010

A mannanase gene (man26B) was obtained from a sea bacterium, Paenibacillus sp. BME-14, through the constructed genomic library and inverse PCR. The gene of man26B had an open reading frame of 1,428 bp that encoded a peptide of 475- amino acid residues with a calculated molecular mass of 53 kDa. Man26B possessed two domains, a carbohydrate binding module (CBM) belonging to family 6 and a family 26 catalytic domain (CD) of glycosyl hydrolases, which showed the highest homology to Cel44C of P. polymyxa (60% identity). The optimum pH and temperature for enzymatic activity of Man26B were 4.5 and $60^{\circ}C$, respectively. The activity of Man26B was not affected by $Mg^{2+}$ and $Co^{2+}$, but was inhibited by $Hg^{2+},\;Ca^{2+},\;Cu^{2+},\;Mn^{2+},\;K^+,\;Na^+$, and $\beta$-mercaptoethanol, and slightly enhanced by $Pb^{2+}$ and $Zn^{2+}$. EDTA did not affect the activity of Man26B, which indicates that it does not require divalent ions to function. Man26B showed a high specific activity for LBG and konjac glucomannan, with $K_m,\;V_{max}$, and $k_{cat}$ values of 3.80 mg/ml, 91.70 ${\mu}mol$/min/mg protein, and 77.08/s, respectively, being observed when LBG was the substrate. Furthermore, deletion of the CBM6 domain increased the enzyme stability while enabling it to retain 80% and 60% of its initial activity after treatment at $80^{\circ}C$ and $90^{\circ}C$ for 30 min, respectively. This finding will be useful in industrial applications of Man26B, because of the harsh circumstances associated with such processes.

• 대한화학회지
• /
• 제56권2호
• /
• pp.236-245
• /
• 2012

The main mycobacterial infection in human is tuberculosis caused by Mycobacterium tuberculosis. Tuberculosis is the leading infectious cause of death in the world. Therefore there is continuing and compelling need for new and improved treatment for tuberculosis. The entire logic towards design of new compounds containing 4-hydroxy-N'-(1,3-thiazoldin- 2-yldene)benzohydrazide moiety is basically for superior antimycobacterial activity. The recent advances in QSAR and computer science have provided a systematic approach to design a structure of any compound and further, the biological activity of the compound can be predicted before synthesis. The 3D-QSAR studies for the set of 4-hydroxy-N'-(1,3-thiazoldin- 2-yldene)benzohydrazide and their derivatives were carried out by using V-life MDS (3.50). The various statistical methods such as Multiple Linear Regression (MLR), Partial Least Square Regression (PLSR), Principle Component Regression(PCR) and K nearest neighbour (kNN) were used. The kNN showed good results having cross validated $r^2$ 0.9319, $r^2$ for external test set 0.8561 and standard error of estimate 0.2195. The docking studies were carried out by using Schrodinger GLIDE module which resulted in good docking score in comparison with the standard isoniazid. The designed compounds were further subjected for synthesis and biological evaluation. Antitubercular evaluation of these compounds showed that (4.a), (4.d) and (4.g) found as potent inhibitor of H37RV.

• 한국산업융합학회 논문집
• /
• 제6권1호
• /
• pp.81-86
• /
• 2003

For the simultaneous removal of $SO_2$/NOx from an iron-ore sintering plant, industrial plasma experiments have been conducted with a flue gas flow rate of $5,000Nm^3/hr$. The maximum 40kW power using the magnetic pulse compression (MPC) system generates a peak value of 100-150kV pulse voltage with its risetime of 200nsec and full width at half maximum (FWHM) of 500nsec, and with a frequency <300Hz. The plasma reactor module adopts a wire-plate structure with a gap of 200-400mm ID between plates. Initial concentrations of $SO_2$ and NOx were around 100-150ppm, respectively in the presence of 15% $O_2$ and <10% $H_2O$. Various reaction parameters such as specific energy ($Whr/Nm^3$), $NH_3$ injection with corona discharge, flow rate and injection of hydrocarbons were investigated for $SO_2$/NOx removal characteristics. About 90/65% of $SO_2$/NOx were simultaneously removed with a specific energy of $3.0Whr/Nm^3$ when both $NH_3$ and hydrocarbons were injected. Practical implications that the pilot-scale plasma results provide are further discussed.

• 한국표면공학회지
• /
• 제48권6호
• /
• pp.297-302
• /
• 2015

Nanocrystalline diamond(NCD) coated aluminium plates were prepared and applied as heat sinks for LED modules. NCD films were deposited on 1 mm thick Al plates for times of 2 - 10 h in a microwave plasma chemical vapor deposition reactor. Deposition parameters were the microwave power of 1.2 kW, the working pressure of 90 Torr, the $CH_4/Ar$ gas ratio of 2/200 sccm. In order to enhance diamond nucleation, DC bias voltage of -90 V was applied to the substrate during deposition without external heating. NCD film was identified by X-ray diffraction and Raman spectroscopy. The Al plates with about 300 nm thick NCD film were attached to LED modules and thermal analysis was carried out using Thermal Transient Tester (T3ster) in a still air box. Thermal resistance of the module with NCD/Al plate was 3.88 K/W while that with Al plate was 5.55 K/W. The smaller the thermal resistance, the better the heat emission. From structure function analysis, the differences between junction and ambient temperatures were $12.1^{\circ}C$ for NCD/Al plate and $15.5^{\circ}C$ for Al plate. The hot spot size of infrared images was larger on NCD/Al than Al plate for a given period of LED operation. In conclusion, NCD coated Al plate exhibited better thermal spreading performance than conventional Al heat sink.

• Transactions on Electrical and Electronic Materials
• /
• 제12권6호
• /
• pp.262-266
• /
• 2011

A simulation study of a current-mode direct current (DC)-DC boost converter is presented in this paper. This converter, with a fully-integrated power module, is implemented by using bipolar complementary metal-oxide semiconductor (BiCMOS) technology. The current-sensing circuit has an op-amp to achieve high accuracy. With the sense metal-oxide semiconductor field-effect transistor (MOSFET) in the current sensor, the sensed inductor current with the internal ramp signal can be used for feedback control. In addition, BiCMOS technology is applied to the converter, for accurate current sensing and low power consumption. The DC-DC converter is designed with a standard 0.35 ${\mu}m$ BiCMOS process. The off-chip inductor-capacitor (LC) filter is operated with an inductance of 1 mH and a capacitance of 12.5 nF. Simulation results show the high performance of the current-sensing circuit and the validity of the BiCMOS converter. The output voltage is found to be 4.1 V with a ripple ratio of 1.5% at the duty ratio of 0.3. The sensing current is measured to be within 1 mA and follows to fit the order of the aspect ratio, between sensing and power FET.

• 핵의학기술
• /
• 제13권3호
• /
• pp.147-151
• /
• 2009

Purpose: 2-[$^{18}F$]Fluoro-2-deoxy-D-glucose ([$^{18}F$]FDG) particularly plays as a important role in Positron Emission Tomography (PET) imaging in nuclear medicine. Domestic [$^{18}F$]FDG auto synthesizers are installed in Seoul National University Bundang Hospital (SNUBH) at June 2008, these modules were known that it's synthetic yields were guaranteed in average $45{\pm}5%$ so far. To improve yields and convenience of domestic [$^{18}F$]FDG auto synthesizer, numerous trials in reaction time, base concentration, pressure and temperature were performed to increase [$^{18}F$]FDG yields. Materials and Methods: Several synthetic factors (temperature, time and pressure) and shortcoming were corrected based on many evaporation test. Syringe dispensing of tetra-butylammonium bicarbonate (TBAB) was replaced with micro pipette to prepare tetrabutyl ammonium fluoride salt ([$^{18}F$]TBAF). Troublesome refill of liquid nitrogen every 2 hours which was used to protect vacuum system was changed to charcoal cartridge, base guard filter. To monitor the volume of delivered $[^{18}O]OH_2$ from cyclotron by surveillance camera, we set up the volumetric vial on the cover of the module. In addition to, the recovery vial was added in [$^{18}F$]FDG production system to recover [$^{18}F$]FDG loss due to the leak of valve ($V_{13,14}$) in [$^{18}F$]FDG purification process. Results: When we used micro pipette for adding TBAB ($30\;{\mu}L$ in 12% $H_2O$ in acetonitrile), this quantitative dispensation has enabled to improve $5.5{\pm}1.7%$ residual fluorine-18 activity in fluorine separation cartridge compared to syringe adding. Besides, the synthetic yields of [$^{18}F$]FDG has increased $58{\pm}2.6%$ (n=19), $58{\pm}2.9%$ (n=14), $60%{\pm}2.5%$ (n=17) for 3 months. The life cycle of charcoal cartridge and base vacuum was 3 months prior to filling liquid nitrogen every 2 hours and additional side separator can prevent pump corrosion by organic solvent. After setting of volumetric indicator vial, the operator can easily monitor the total volume of irradiated $[^{18}O]OH_2$ from cyclotron. The recovery vial can be used for the stabilizer when an irregular [$^{18}F$]FDG loss was generated by the leak of valves ($V_{13,14}$). Conclusions: We has optimized appropriate synthetic conditions (temperature, time, pressure) in domestic [$^{18}F$]FDG auto synthesizer. In addition to, the remodeling with several accessories improve yields of domestic [$^{18}F$]FDG auto synthesizer with reliable reproducibility.