• 한국작물학회지
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• 제45권5호
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• pp.343-346
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• 2000

Rye breeding using F$_1$ hybrid began about 30 years ago, when cytoplasmically inherited forms of male sterility (CMS) and corresponding nuclear restorers were detected. It is very important to produce inbred lines for making hybrid lines because of strong self-incompatibility in rye. Among the 456 rye germplasms used in hybrid breeding scheme, 24 lines (5.3%) had the above 60% of self-fertility, and six lines of them were selected for their good agronomic characteristics and were used for subsequent inbreeding program. The average self-fertility of selected six lines was 78.4%, ranging from 72.2 to 99.5%. Genetic analysis for the self-fertility using $F_2$ populations showed that the segregation of self-fertile and sterile plants in F$_2$populations could be fit into 3 to 1 ratio suggesting self-fertility in rye be controlled by one major gene. The four different self-fertile lines, PI237923, 5C11, 5G5 and Florida black, had the same self-fertility gene because their F$_2$ plants showed almost the same self-fertility as their parents and showed no genetic segregation.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권1호
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• pp.41-46
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• 2004

A breeding programme was initiated by utilizing a robust bivoltine breed CSR$_{18}$ and a polyvoltine breed Cambodge with the main objective of developing robust polyvoltine silkworm breeds/hybrids. At F$_1$ and F$_2$, parthenogenetic development was induced following warm water treatment of eggs at 46$^{\circ}C$ for 18 min followed by two backcrosses with Bl$_{67}$, an evolved polyvoltine breed. The newly developed breed was subjected for hybrid study using eight hybrid combinations in the laboratory at F$_{8}$ generation. F$_1$ hybrids between newly developed breed Bl$_{67}$ (Pg) and promising bivoltine breeds exhibited their superiority by expressing significant hybrid vigour for several economic characters like cocoon yield/10,000 larvae, cocoon weight, cocoon shell weight, cocoon shell ratio and denier. Study on cocoon shape variability revealed that cocoons of all the F$_1$ hybrids except BL$_{67}$ (Pg)${\times}$NB$_4$D$_2$ were found comparatively uniform in shape.pe.

• Journal of Microbiology
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• 제38권2호
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• pp.113-116
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• 2000

Escherichia coli F plasmid partition apparatus is composed of two trans-acting proteins (SopA and SopB) and one cis-acting DNA sequence (sopC). The SopB-sopC complex has been suggested to serve a centromere-like function through its interaction with chromosomally encoded proteins which remain to be identified. In this paper, we are introducing a new yeast 1.5-hybrid system which assembles the two-hybrid and one-hybrid system as a mean to find and additional component of the F plasmid partition system, interacting with DNA (sopC)-bound SopB protein. The results indicates that this system is a promising one, capable of selecting an interacting component.

• 한국연초학회지
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• 제7권2호
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• pp.111-121
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• 1985

The present study was carried out to obtain some basic informations of the breeding of tobacco varieties. Genetically divergent 8 varieties, 3 flue-cured, 2 burley and 3 sun-cured tobaccos, were used in half diallel cross. In order to analyze the heterosis, combining abilities, modes of inheritance and correlations for some agronomic and chemical characters, 8 parents and 28 $F_2$ were tested. The results obtained were summarized as follows: The percentages of heterosis for yield, stem diameter, internode length and total sugar content in $F_2$ hybrid were positive , where-as those for the other characters were negative. Yield had significant positive genotypic and phenotypic correlations with plant height, days to flower, leaves per plant, leaf length, leaf width and leaf shape index (Leaf length/leaf width). General (GCA) and specific combining abilities (SCA) for all characters of $F_2$ hybrid were significant. In the effect of GCA, yield was greater in combinations of Bulgaria and Xanthi, days to flower and leaves per plant were those of Burley 21 and Va 528, leaf length and width were those of Coker 139, respectively. In the effect of SCA, the major part of characters were greater than those of others in the combinations of Burley 21, Va 528, Hicks.

• 한국산림과학회지
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• 제66권1호
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• pp.109-117
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• 1984

잡종채종원상(雜種採種園上)의 리기다소나무 49가계(家系)로부터 종자(種字)를 채취하여 종자(種字)의 배유(胚乳) 및 배(胚)에 대한 Aspartate aminotransferase(AAT), Glutamate dehydrogenase(GDH) 및 Leucine aminopeptidase(LAP)등의 Allozyme 변이(變異)를 조사하여 다음과 같은 결과를 얻었다. 이들 세 가지 Allozyme system에서 AAT에 5개, GDH에 1개 및 LAP에 2개, 모두 8개의 유전자좌(遺傳子座)(Locus)가 발견되었으며 GDH를 제외한 모든 유전자좌에서 Allozyme Polymorpshism을 발견하였다. 각 유전자좌에 있어서 평균 대입유전자(對立遺傳子) 수(數)는 종자모수집단(種子母樹集團)에서 2.33개, 차대집단(次代集團)에서 2.67개였다. 평균이형접합성(平均異型接合性) 종자모수집단이 0.235, 차대집단(次代集團)이 0.238이었고 유전자의 유전적(遺傳的) 다양성(多樣性)은 종자모수집단이 5.409, 차대집단(次代集團)이 5.569로서 같은 수종의 다른 집단 또는 다른 침엽수 수종에 비하여 비교적 낮은 값을 나타냈다. Allozyme분석에 의하여 잡종채종원의 리기다소나무에 있어서 일대잡종(一代雜種) 종자(種子) 발생율(發生率)을 추정해 본 결과 일대잡종 종자의 발생빈도는 0.77%로서 묘포에서 조사한 일대잡종묘의 발생율 0.73%와 거의 일치하였다. 잡종채종원상의 종자모수 리기다소나무 및 이대 차대들 Allozyme변이에 있어서 Wahlund 효과(效果), 비교적 높은 수준의 자가수정(自家受精) 및 Non-random mating 등의 가능성이 발견되어 이들에 대한 Allozyme 변이 연구에 깊은 주의가 필요하다.

• 한국산림과학회지
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• 제43권1호
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• pp.1-5
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• 1979

Poulusp alba P. glandulosa와 그의 양친종(兩親種)에 대(對)하여 엽병(葉柄)의 중간부위(中間部位)의 유관속(維管束)의 수(數)와 배열상태(配列狀態)를 조사하여 다음과 같은 결과(結果)를 얻었다. 1) 유관속(維管束)의 수(數)와 배열상태(配列狀態)의 변이(變異)는 엽병(葉炳)의 중간부위(中間部位)가 엽신측부위(葉身側部位)보다 변화(變化)가 적었다. 2) 유관속(維管束)의 배열(配列)과 수(數)의 변이(變異)는 한 개체(個體)에서, 또는 동일(同一) clone의 개체(個體)에 따라서, 그리고 동일(同一) 수종(樹種)의 개체(個體)에 따라서 다소(多少)의 변이(變異)가 있다. 3) P. alba${\times}$P. glandulosa의 $F_1$의 유관속형(維管束型)은 5 type이 있다. 그중(中) 26.7%는 P. alba와과 동일형(同一型)이고, 13.3%는 P. glandulosa와 동일형(同一型)이고, 그리고 53.3%은 유전(遺傳)에 의(依)하여 연유(緣由)된 $F_1$형(型)이다. 4) P. alba형(型)을 가진 clone number는 66-20-1, 66-6-8, 65-22-11, 64-6-44, P.이고, 그 중(中)에는 P. tomentiglandulosa와 유사(類似)한 clone도 있었다. P. glandulosa형(型)의 clone number는 65-95, 66-14-93,이다. $F_1$형(型)의 clone은 66-15-3, 67-6-3, 65-22-4, 66-26-55, 68-1-54, 66-14-99, 65-29-19, 66-25-5이다.

• International Journal of CAD/CAM
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• 제3권1_2호
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• pp.13-17
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• 2003

Hybrid method of representing geometric entities as combination of boundary (B-rep) and functional (F-rep) representations is presented which can be used as a basis of solid modeling kernel. It contains whole functionality of classic B-rep kernel, and also supports enveloping (sweep of solid body). Principles and keysolutions are considered. Example of a real task that comes from gearing is provided.

• 화훼연구
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• 제17권4호
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• pp.279-284
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• 2009

불임인 종간잡종 OA-1 $F_1$ hybrid(Oriental hybrid 'Mero Star' ${\times}$ Asiatic hybrid 'Connecticut King')의 임성을 회복하기 위해 카페인을 0.1, 0.3, 0.5%의 농도로 화뢰 내부에 직접 주입하였고 카페인 처리 후 OA-1 $F_1$ hybrid의 임성을 개화시 확인하였다. 임성을 회복한 $F_1$에 Asiatic hybrid 'Lanzarote'의 화분을 수분시켜 그 후대를 얻었다. 그 결과 0.3% caffeine 처리구는 16개의 자방 중 7개의 배를 치상하여 5개의 식물체를 획득할 수 있었다. 0.5% 처리구에서는 1개의 식물체만 얻었고 0.1% 처리구에서는 식물체를 얻을 수 없어 적정 카페인 처리 농도는 0.3%임을 확인할 수 있었고 이로부터 2n gametes로 예상되는 화분이 51%나 생성되었다. 교잡종 OA-2 ('Romero Star' ${\times}$ 'Lady Rosa')와 OA-3('Expression' ${\times}$ 'Lady Rosa')에 온도 단독 또는 caffeine과 온도를 병행하여 재식 후 8주-13주 동안 $13^{\circ}C$와 $28^{\circ}C$로 변온처리하여 발아율을 측정한 결과, 온도만 처리한 경우나 온도와 caffeine을 동시에 처리한 경우 간에는 큰 차이가 없었다. 적정 caffeine(0.3%)을 처리한 OA-1에 Asiatic hybrid 'Lanzarote'와 Oriental hybrid 'Sorbonne'을 각각 주두수분법으로 정역교배한 결과, A('Lanzarote') ${\times}$ OA-1 또는 OA-1 ${\times}$ A간 교배조합은 O('Sorbonne') ${\times}$ OA-1 또는 OA-1 ${\times}$ O 교배조합보다 식물체 획득률이 양호하였다. A ${\times}$ OA-1 또는 OA-1 ${\times}$ A로부터 획득된 후대는 GISH 분석에 의해 모두 3배체로 확인되었다.

• Mycobiology
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• 제34권2호
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• pp.92-98
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• 2006

A total of 34 species belonging to 21 genera of fungi were recorded on passion fruits of both pure and hybrid origin in Uganda, however, the pure type exhibited wider spectrum (28 species and 16 genera) than the hybrid type (21 & 15). Also, yeasts (unidentified and Rhodotorula mucilaginosa) were also encountered in high numbers. Moreover, the mean count of all mycobiota obtained from the pure type was higher than that of hybrid, despite the bigger size of the later. Members of yeasts and Cladosporium followed by Phoma, Penicillium, Fusarium and Alternaria species dominated on passion fruits of pure origin, while only C. cladosporioides, F. solani and yeasts dominated on the hybrid type. Treatment with Na-hypochlorite exhibited inhibitory effects on the total mycobiotic propagules as well as the dominant species from fruits of both types. The current results, therefore, suggest the use of Na-hypochlorite to control the post-harvest mycobiota associated with passion fruits. Regarding the mycobiota contaminating passion juice, yeasts were found to be the major contaminants with Candida parapsilosis being the most common. Moulds constituted only a minor proportion with Acremonium strictum followed by Fusarium chlamydosporum, F. moniliforme, F. acuminatum and F. solani as the most dominant species. In the heat-treated juice samples, the counts of the most commonly encountered mycobiota (both yeasts and molds) were significantly inhibited or completely eliminated. Some unidentified Bacillus species were also recovered from the juice, however, their counts in the heated samples were increased but insignificantly.

• 식물분류학회지
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• 제32권3호
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• pp.293-299
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• 2002

새로이 명명된 완도호랑가시나무(Ilex $\times$ wandoensis C. F. Miller & M. Kim hybrid nov.)는 전남 완도군 완도에서 채집하였다. 완도호랑가시나무는 호랑가시나무(I. cornuta Thunb.)와 감탕나무(I. integra Thunb.)의 자연교잡을 통해 형성된 분류군이다. 따라서 가시형 엽연과 평평한 표면을 가진 신분류군은 사각형으로 뒤틀린 엽연에 강한 가시형 거치와 볼록한 표면을 가진 호랑가시나무와 전연이며 평평한 표면을 가진 감탕나무의 중간형질을 가지고 있다. 또한 완도호랑가시나무는 소교목이고 1.0-1.4cm의 엽병을 가진 점에서 관목이고 0.4-0.6cm의 엽병을 가진 호랑가시나무와 키가 큰 교목이고 1.5-2.0cm의 엽병을 가진 감탕나무와 뚜렷이 구별이 된다.