• Title/Summary/Keyword: $E_rC_{50}$

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Fracture Resistances of Y2O3 Particle Dispersion Strengthened 9Cr Steel at Room Temperature and High Temperatures (Y2O3 입자 분산강화 9Cr 강의 상온 및 고온 파괴저항특성)

  • Yoon, Ji Hyun;Kang, Suk Hoon;Lee, Yongbok;Kim, Sung Soo
    • Korean Journal of Metals and Materials
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    • v.50 no.1
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    • pp.1-7
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    • 2012
  • The fracture resistance and tensile properties of $Y_2O_3$ oxide dispersion strengthened steel containing 9 wt% Cr(9Cr-ODS) were measured at various temperatures up to $700^{\circ}C$. The fracture characteristics were compared with those of commercial E911 ferritic/martensitic steel. The strength of 9Cr-ODS was at least 30% higher than that of E911 steel at the test temperatures below $500^{\circ}C$. The strength difference between the two materials was almost diminished at $700^{\circ}C$. 9Cr-ODS showed cleavage fracture behavior at room temperature and unstable crack growth behaviors at $300^{\circ}C$ and $500^{\circ}C$. The J-R fracture resistance of 9Cr-ODS was much lower than that of E911 steel at all temperatures. It was deduced that the coarse $Cr_2O_3$ particles that were formed during the alloying process provided the crack initiation sites of cleavage fracture in 9Cr-ODS.

Selective Detection of Campylobacter jejuni, C. coli, Arcobacter butzleri and Helicobacter pylori by Polymerase Chain Reaction (Campylobacter jejuni, C. coli, Arcobacter butzleri와 Helicobacter pylori의 PCR에 의한 분리검출)

  • Lee, Young-Duck;Park, Jong-Hyun
    • Korean Journal of Food Science and Technology
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    • v.34 no.6
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    • pp.1134-1139
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    • 2002
  • Campylobacter, Arcobacter, and Helicobacter, classified into the same rRNA superfamily VI by taxonomy, cause food-borne diseases, stomach ulcer, and gastric cancer. To detect each strain selectively from contaminated foods, PCR, multiplex-PCR, and restricion fragment length polymorphism (RFLP) were applied on Campylobacter, Arcobacter, and Helicobacter. The same PCR products could be detected using CHA primer targeted for 16S rRNA of Campylobacter, Arcobacter, and Helicobacter. To detect C. jejuni and C. coli from A. butzleri and H. pylori, pg50/pg3 primer targeted for fla A gene was used, and for A. butzleri, Arco2/Butz primer targeted for 23S rRNA was utilized. For H. pylori detection, icd1/icd2 primer targeted for isocitrate dehydrogenase gene was employed, and JEJ1/JEJ2 primer targeted for ceuE gene was effective for C. jejuni detection from the three strains. C. jejuni, C. coli could be separated from A. butzleri and H. pylori through PCR-RFLP using restriction enzyme Dde I. Such primers would be effective for detecting each strain selectively through PCR when C. jejuni, C. coli, A butzleri and H. pylori are contaminated together.

Pure red-cell aplasia [P.R.C.A.] with thymoma: a case report (흉선종을 동반한 적혈구 무형성증 1례 보)

  • Sun, Kyung;Lee, Chol-Sae;Baek, Kwang-Je;Kim, Yo-Han;Kim, Hark-Jei;Kim, Hyoung-Mook
    • Journal of Chest Surgery
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    • v.17 no.4
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    • pp.796-803
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    • 1984
  • Pure Red-Cell Aplasia [P.R.C.A.] is rare disease characterized by absence of erythroid precursors in the bone marrow, normocytic normochromic anemia with profound reticulocytopenia in the peripheral blood, and relatively or completely spared granulopoiesis and thrombopoiesis. The association rates of P.R.C.A. with Thymoma is approximately 50%, but only 5-10% of all patients with a Thymoma have a P.R.C.A.. P.R.C.A. is thought to be a variety of autoimmune disease, and humoral inhibitor, i.e. IgG, has been demonstrated experimentally. Its treatments such as thymectomy, immunosuppressants, steroid, androgenic hormone, and splenectomy have been tried but the result is not satisfactory and the prognosis is poor. We experienced a case of P.R.C.A. with Thymoma treated with thymectomy and postoperative steroid therapy, and which showed good postoperative recovery clinically and hematologically.

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The Effect of N-Alkyloxycarbonyl Group on the Anticonvulsant Activities of N-Alkyloxycarbonyl-${\alpha}$-aminoglutarimides

  • Son, Ki-Chun;Jung, Kyung-Im;Kim, Min-Jeong;Lee, Jae-Won;Choi, Jong-Won;Lee, Eung-Seok;Park, Min-Soo
    • Archives of Pharmacal Research
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    • v.21 no.6
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    • pp.764-768
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    • 1998
  • In connection with the development of new anticonvulsant agents with a broad spectrum, we reported that N-Cbz-alpha-aminoglutarimides, combining common structures of othe r anticonvulsants such as N-CO-C-N and cyclic imides in a single molecule, showed significant anticonvulsant activities in the MES (maximal electroshock seizure) and PTZ (pentylenetetrazole induced seizure) tests. In these studies, a series of (R) and (S) N-alkyloxycarbonyl-alpha-aminoglutarimides 7a-7e and 8a-8e, which were substituted with various alkyloxycarbonyl group instead of Cbz group, were prepared from the corresponding (R) and (S) N-Cbz-glutamic acid 3 and 4, and were evaluated with their anticonvulsant activities against the MES and PTZ tests, including neurotoxicity, in order to define the effect of N-alkyloxycarbonyl group on the anticonvulsant activities of N-alkyloxycarbonyl-${\alpha}$-aminoglutarimides. Among them, (S)N-4-nitrobenzyloxycarbonyl-${\alpha}$-amino-N-methylglutarimide 8e was the most active in MES ($ED_{50}$=35.6mg/kg, PI=2.7) and PTZ tests ($ED_{50}$=15.6, PI=6.1). Interestingly, (R) and (S) N-4-nitrobenzyloxycarbonyl-${\alpha}$-amino-N-methylglutarimide 7e and 8e and (R) N-phenoxycarbonyl-${\alpha}$-amino-N-methylglutrimide 7d showed significant anti-convulsant activities in both the MES and PTZ tests and other compounds showed anticonvulsant activities in only the PTZ test. In addition, it was found that their anticonvulsant activities were dependent on their stereochemistries and N-substituted alkyloxycarbonyl groups.

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Comparison of the Effects of CPR Training using Little Anne, BRAYDEN and HeartiSense (Little Anne, BRAYDEN, HeartiSense를 이용한 심폐소생술 교육효과 비교)

  • Kim, in-Hyeon;Choi, Uk-Jin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.11
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    • pp.290-296
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    • 2017
  • Purpose: The purpose of this study was to compare the effects of CPR training according to the mannequin used for CPR training using Little $Anne^{(R)}$, $BRAYDEN^{(R)}$, and $HeartiSense^{(R)}$. Method: Research subjects were 90 firefighters who were not paramedics. They were divided into three groups, A, B, and C. Theoretical education was conducted for 50 minutes, and practical education was conducted in groups of three for 50 minutes, using Little $Anne^{(R)}$ for 30 people in Group A; $BRAYDEN^{(R)}$ for 30 people in Group B; and $HeartiSense^{(R)}$ for 30 people in Group C. The results of CPR were printed out using Resusci Anne $SkillReporter^{(R)}$, and the subjects were asked to fill in a questionnaire. Results: The following significant differences were noted: satisfaction index according to the mannequin, $x^2=81.050$, p<.000; reason for satisfaction, $x^2=91.050$, p<.000; ratio of accurate chest compression, F=5.087, p<.004; and ratio of accurate ventilation, F=10.54, p<.000. Conclusion: For the subjects who require accurate and efficient CPR training, it is judged that a mannequin that provides information to allow corrections in real-time would be effective, and have a high educational effect.

Biological Activity and Acute Toxicity of the Multimers of CJ500011 Recombinant Human Granulocyte Colony-stimulating Factor (rHuG-CSF), Produced in E. coli (재조합 사람 과립구 콜로니 자극인자인 C,J50001의 중합체의 생물학적 활성과 급성독성에 관한 연구)

  • 하석훈;이현수;김기완;정종상;김달현
    • Biomolecules & Therapeutics
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    • v.6 no.1
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    • pp.89-94
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    • 1998
  • CJ50001 is a recombinant human granulocyte colony-stimulating facto, (rHuG-CSF) that stimulates the formation of neutrophils from bone marrow stem cells. It was produced in E. colt and purified through refolding and several processes. We produced CS970125(300) using purified C150001 and additives in order to test the stability of CJ50001. When CS970125(300) was stored at 50'S for more than 1 week, high molecular weight proteins were formed and those proteins were detected by non-reducing SDS-PAGE, gel filtration HPLC, and Western blot. Those proteins showed single band at the same position of CJ50001 in reducing SDS-PAGE. These data indicated that those high molecular weight proteins were the multimers of C150001. In biological assays, iu viro and in viro, the multimers did not have biological activity and inhibitory action to that of CJ 50001. The mutimers did not induce toxicity in mice and rats in acute toxicity test. These results suggest that if Cs970125(300) containing CJ50001 is stored at 5$0^{\circ}C$, CJ50001 will be the multimers that do not have biological activity and inhibitory effect to CJ50001 and do not induce acute toxicity.

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A Comparative Study for the Microwave Surface Resistances of $YBa_2$$Cu_3$$O-{7-$\delta$}$ Films Measured with a Microstrip Resonator and a Inutile-loaded Cavity Resonator (마이크로스트립 공진기와 Rutile-loaded Cavity 공진기로 측정한 $YBa_2$$Cu_3$$O-{7-$\delta$}$박막의 마이크로파 표면저항 비교 연구)

  • O. K. Kwon;H. J. Kwon;Lee, J. H.;Jung Hur;Lee, Sang-Young
    • Progress in Superconductivity
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    • v.2 no.2
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    • pp.86-91
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    • 2001
  • Temperature dependences of the unloaded Q(Q$_{0}$) and the resonant frequency ( $f_{0}$) of YB $a_2$C $u_3$ $O_{7-{\delta}}$ (YBCO) microstrip ring resonators and rutile-loaded cylindrical cavity resonators were measured at low temperatures. Dc magnetron-sputtered YBCO films grown on Ce $O_2$-buffered r-cut sapphire (CbS) substrates were used fur this purpose. The surface resistances ( $R_{s}$) of YBCO films measured by both a microstrip ring resonator and a TE $01\delta$/ mode rutile-loaded cylindrical cavity resonator are compared with each other. It turned out that the values of $R_{s}$ measured by the microstrip resonator technique are comparable to those by the rutile-loaded resonator technique at temperatures lower than ~50 K. However, above 50 K, the $R_{s}$ measured by the microstrip resonator technique appeared higher according to the temperature. Our results show that the current crowding effects near the edge of a microstrip resonator become more significant at temperatures near the critical temperature.emperature.e.e.e.e.e.e.

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Fermentation Characteristics and Microbial Diversity of Tropical Grass-legumes Silages

  • Ridwan, Roni;Rusmana, Iman;Widyastuti, Yantyati;Wiryawan, Komang G.;Prasetya, Bambang;Sakamoto, Mitsuo;Ohkuma, Moriya
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.4
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    • pp.511-518
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    • 2015
  • Calliandra calothyrsus preserved in silage is an alternative method for improving the crude protein content of feeds for sustainable ruminant production. The aim of this research was to evaluate the quality of silage which contained different levels of C. calothyrsus by examining the fermentation characteristics and microbial diversity. Silage was made in a completely randomized design consisting of five treatments with three replications i.e.: R0, Pennisetum purpureum 100%; R1, P. purpureum 75%+C. calothyrsus 25%;, R2, P. purpureum 50%+C. calothyrsus 50%; R3, P. purpureum 25%+C. calothyrsus 75%; and R4, C. calothyrsus 100%. All silages were prepared using plastic jar silos (600 g) and incubated at room temperature for 30 days. Silages were analyzed for fermentation characteristics and microbial diversity. Increased levels of C. calothyrsus in silage had a significant effect (p<0.01) on the fermentation characteristics. The microbial diversity index decreased and activity was inhibited with increasing levels of C. calothyrsus. The microbial community indicated that there was a population of Lactobacillus plantarum, L. casei, L. brevis, Lactococcus lactis, Chryseobacterium sp., and uncultured bacteria. The result confirmed that silage with a combination of grass and C. calothyrsus had good fermentation characteristics and microbial communities were dominated by L. plantarum.

Replication origin (ori) of R-plasmid pSBK203 Isolated from Staphylococcus aureus DHI (Staphylococcus aureus DH1에서 분리한 R-plasmid pSBK203상의 복제개시 부위 ori에 관한 연구)

  • Min, Kyung-Il;Byeon, Woo-Hyeon
    • Korean Journal of Microbiology
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    • v.32 no.3
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    • pp.186-191
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    • 1994
  • The origin of the leading strand replication (ori) and of lagging strand replication (M-O) of R-plasmid pSBK203 was identified and its base sequence was determined. About 50 bp of ori sequence residues overlapped with the structural gene of rep. Sequence comparison reveals that pSBK-ori shares obvious identities with those of pT181 family and consists of two regions, one is conserved and the other is variable region. Of two palindrome sequence located one after another in upstream region of rep gene, palA' instead of palA which shares sequence homology with diverse family of plasmids such as pOX6, pC194, and pE194 seems to act as a signal for conversion of primarily replicated ssDNA to dsDNA (minus origin (M-O)).

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Isolation and Characterization of Catalase-producing Bacteria from Soil (토양으로부터 카탈라제 생산균의 분리 및 특성)

  • Han, Kyung-Ah;Rhee, Jong-Il
    • KSBB Journal
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    • v.24 no.6
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    • pp.508-514
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    • 2009
  • This study investigated the production of catalase from Bul-kyo soil bacteria through fermentation process. Isolation and selection of bacteria was performed through chemical and physiological analysis. Catalases were produced from bacteria which belong to 3 different species (Bacillaceae bacterium BKBChE-1, Bacillus sp. BKBChE-2, Bacillus flexus BKBChE-3) confirmed by using 16S rDNA sequence method. The catalases were found to be stable in the temperature range of $30^{\circ}C-60^{\circ}C$ for BKBChE-1, BKBChE-2 and BKBChE-3 and also in the pH range of 9.0-12.0 for BKBChE-1 and BKBChE-3. Long-term stability of the catalases was about 20 days at $4^{\circ}C$. However, BKBChE-2 has kept its activity over 30 days at $4^{\circ}C$.