• Title/Summary/Keyword: $E_iC_{50}$

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17 beta-Estradiol Increases Peak of $\textrm{Ca}^{2+}$ Current in Mouse Early Embryo (에스트로겐이 생쥐 초기배의 $\textrm{Ca}^{2+}$ 전류에 미치는 영향)

  • 강다원;신용원;김은심;홍성근;한재희
    • Journal of Embryo Transfer
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    • v.16 no.2
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    • pp.79-89
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    • 2001
  • Steroid hormones control the expression of many cellular regulators, and a role thor estrogen in mouse oocytes has been well documented. The preovulatory $E_2$increment is generally accepted as the endocrine process regulating induction of in vivo oocyte maturation To address whether the activity of the T-type $Ca^{2+}$ channel is altered by 17 beta-estradiol ( $E_2$), we examined the actions of $E_2$on the calcium channel of mouse oocytes and early embryos. Oocrtes were collected from the oviduct of mice treated with pregnant mare's serum gonadotropin (PMSG) and human choronic gonadotropin (hCG). Whole cell voltage clamp technique and confocal microscopy were used to examine that $E_2$increase intracellular $Ca^{2+}$ concentration ([C $a^{2+}$]$_{i}$ ) via voltage dependent $Ca^{2+}$ channel (VDC) and estrogen receptor (FSR), and $E_2$concentration by the use of radioimmunoassay (RIA) were examined in mouse. The results obtained were as follows: The peak of $Ca^{2+}$ current induced by $E_2$increased 122% to 1.50$\pm$0.03 nA from 1.23$\pm$0.21 nA (n=15) in the presence of 5 mM extracellular $Ca^{2+}$ concentration ([C $a^{2+}$]$_{o}$ ). The increased $Ca^{2+}$ current was temporally associated with $Ca^{2+}$ transients. The intracellular $Ca^{2+}$ level increased 207%~30 s following the addition of 1${\mu}{\textrm}{m}$ $E_2$(relative fluorescence intensity: 836.4$\pm$131.2 for control, n=10, 1736.4$\pm$192.0 in the presence of $E_2$, n=10). $E_2$increased amplitude of $Ca^{2+}$ current and [C $a^{2+}$]$_{i}$ . $E_2$-induced $Ca^{2+}$ current and $E_2$concentration in blood were showed difference on the stage of embryo. These results suggest that $E_2$modulate $Ca^{2+}$ channel to increase $Ca^{2+}$ influx.$Ca^{2+}$ influx.

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RUBBER INCLUSION EFFECTS ON MECHANICAL PROPERTIES OF RUBBER-ADDED COMPOSITE GEOMATERIAL

  • Kim, Yun-Tae;Gang, Hyo-Seb
    • Proceedings of the Korean Geotechical Society Conference
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    • 2010.09c
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    • pp.129-134
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    • 2010
  • This paper investigates effects of rubber inclusion on the strength and physical characteristics of rubber.added composite geomaterial (CGM) in which dredged soils, crumb rubber, and bottom ash are reused for recycling. Several series of test specimens were prepared at 5 different percentages of rubber content (i.e. 0%, 25%, 50%, 75%, and 100% by weight of the dry dredged soil) and three different percentages of bottom ash content (i.e. 0%, 50% and 100% by weight of the dry dredged soil). The mixed soil specimens were subjected to unconfined compression test and elastic wave test to investigate their unconfined compressive strengths and small strain properties. The values of bulk unit weight of the CGM with bottom ash content of 0% and 100% decrease from 14kN/$m^3$ to 11kN/$m^3$ and 15kN/$m^3$ to 12kN/$m^3$, respectively, as rubber content increases, because the rubber had a specific gravity of 1.13. The test results indicated that the rubber content and bottom ash content were found to influence the strength and stress-strain behavior of CGM. Overall, the unconfined compressive strength, and shear modulus were found to decrease with increasing rubber content. Among the samples tested in this study, those with a lower rubber content exhibited sand-like behavior and a higher shear modulus. Samples with a higher rubber content exhibited rubber-like behavior and a lower shear modulus. The CGM with 100% bottom ash could be used as alternative backfill material better than CGM with 0% bottom ash. The results of elastic wave tests indicate that the higher rubber content, the lower shear modulus (G).

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Identification and delegation of indirect care interventions (간접간호중재의 수행 및 위임에 관한 분석)

  • Yom, Young-Hee;Kim, So-In;Cha, Boo-Kyung
    • Journal of Korean Academy of Nursing Administration
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    • v.5 no.2
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    • pp.197-207
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    • 1999
  • The purpose of this research was fourfold: (a) to identify the use rate of the indirect care interventions performed by nurses, (b) to estimate the time to perform each intervention, (c) to identify the indirect care interventions to be delegated to others, and (d) to determine the level of provider preparation needed to delegate indirect care interventions. The sample consisted of 199 nurses working in three hospitals. The Indirect Care Survey developed by the Iowa Intervention Project team was used for data collection. The instrument was translated to Korean and validated by nurse experts. Each of the 26 indirect care interventions were used several times a day. Four interventions (i.e.. Documentation, Shift Report, Specimen Management, and Transport) were performed several times a day by 50% or more of the nurses. The most frequently used intervention was Documentation, followed by the interventions Shift report. Environmental Management, Transport, and Examination Assistance. The least used intervention was Quality Monitoring, followed by the interventions Order Transcription, Referral, Health Care Information Exchange, Multidisciplinary Care Conference, and Product Evaluation. The intervention taking the most time to per-form was Technology Management (155.3 minutes), followed by the interventions Documentation, (122.2 minutes), Delegation (84.4 minutes), Supply management (83.4 minutes), and Preceptor: Student (79.9 minutes), Overall, the nurses reported that they would not delegate to others the majority of the interventions. More than 50% of the nurses would not delegate 21 interventions. Shift Report would not be delegated by 95% of the nurses and Documentation would not be delegated by 92% of the nurses. Caregiver Support would be delegated by 68% of the nurses to family. Three interventions (i.e.. Environmental Management, Examination Assistance, and Transport) would be delegated by more than 50% of the nurses to Nursing Assistant. This study will contributes to determining costs of nursing services and enhancing quality of nursing care. Replication study will be needed with large sample.

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Influence of gas mixture ratio on the secondary electron emission coefficient ($\gamma$) fo MgO single crystals and MgO protective layer in AC PDP

  • Lim, Jae-Yong;Jung, J.M.;Choi, M.C.;Ahn, J.C.;Cho, T.S.;Kim, T.Y.;Kim, S.S.;Jung, M.W.;Choi, S.H.;Kim, S.B.;Ko, J.J.;Kim, D.I.;Lee, C.W.;Seo, Y.;Cho, G.S.;Kang, S.O.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2000.04a
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    • pp.145-147
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    • 2000
  • The secondary electron emission coefficient y of MgO single crystal according to the gas mix-ture ratio of Xe, $N_2$ to Ne have been investigated by $\gamma$-focused ion beam system. It is found that the MgO single crystals of (111) crystallinity has the highest $\gamma$ for operating Ne(Xe) ions ranging from 50eV to 200eV throughout this experiment. And it is found that the $\gamma$ for gas mixtures are much smaller than pure Ne ions.

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Enhancement of Dimensional Stability of Compressed Open Cell Rigid Polyurethane Foams by Thermo-Mechanical Treatment

  • Ahn, WonSool
    • Elastomers and Composites
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    • v.50 no.1
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    • pp.30-34
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    • 2015
  • Thermo-mechanical treatment process of a compressed open-cell rigid polyurethane foam (OC-RPUF), which was fabricated for the vacuum insulation panel (VIP), was studied to obtain an optimum condition for the dimensional stability by the relaxation of compressive stress. Thermo-mechanical deformation of the sample OC-RPUF was shown to occur from about $120^{\circ}C$. Yield stress of 0.36 MPa was shown at about 10% yield strain. And, densification of the foam started to occur from 75% compressive strain and could be continued up to max. 90%. Compression set of the sample restored after initial compression to 90% at room temperature was ca. 82%. Though the expansion occurred to about twice of the originally compressed thickness in case of temperature rise to $130^{\circ}C$, it could be overcome and the dimensional stability could be maintained if the constant load of 0.3 MPa was applied. As the result, a thermo-mechanical treatment process, i.e, annealing process at temperature of $130{\sim}140^{\circ}C$ for about 20 min as is the maximum compressed state at room temperature, should be required for dimensional stability as an optimum condition for the use of VIP core material.

Contactless DC Circuit Breakers Using MOS-controlled Thyristors (전력용 사이리스터 MCT를 이용한 무접점 직류차단기)

  • Sim, D.Y.;Kim, C.D.;Nho, E.C.;Kim, I.D.;Kim, Y.H.;Jang, Y.S.
    • Journal of Power System Engineering
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    • v.4 no.1
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    • pp.45-50
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    • 2000
  • Circuit breakers have traditionally employed mechanical methods to interrupt excessive currents. According to power semiconductor technology advances in power electronic device, some mechanical breakers are replaced with solid state equivalents. Advantages of the contactors using semiconductor devices include faster fault interrupting, fault current limiting, no arc to contain or extinguish and intelligent power control, and high reliability. This paper describes the design of a static $100{\pm}10%V$ and 0 to 50A DC self-protected contactor with 85A "magnetic tripping" and 100A interruption current at $2.2A/{\mu}s$ short circuit of load condition using a new power device the HARRIS MCT (600V-75A). The self-protection circuit of this system is designed by the classical ZnO varistor for energy absorption and turn-off snubber circuit ("C" or "RCD") of the MCT.

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Studies on the Fish Larvae Community in the Sea Around Cheju Island in November, 1986 (제주도 주변 해역의 치자어 군집에 관한 연구-가을철)

  • Yoo, Jae-Myung;Kim, Sung;Lee, Eun-Kyung;Kim, Yong-Uk
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.3 no.2
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    • pp.71-79
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    • 1998
  • Fish larvae and environmental variables were collected in the sea around Cheju Island of Korea in November, 1986. Fish larvae were found at the 43 of 44 stations. Abundance of fish larvae rangεd from 3 to 259 indiv./$10^3\;m^3$ (average 50 indiv./$10^3\;m^3$) Dominant species were Engraulis japonicus, Champsodon snyderi, Gobiidae, Sebastiscus marmoratus, and Synagrops philippinensis, which comprised 56.6% of total fish larvae abundance. 43 stations were c1assified into two groups by c1uster analysis based on the abundance of fish taxa. Most stations of Group I were located in the middle reach of Tsushima Current and had higher number of species and abundance than the sites of Group II which cover the marginal areas of Tsushima Current, Korean Coastal Waters, and coastal water of China. The dominant taxa in the sites of the Group I were E. japonicus, Gobiidae, S. philippinensis, Aulops japonicus, Bregmaceros spp., Benthosema pterotum, Lampanctus spp., etc., all of which are typical warm-water species. Therefore, Tsushima Current seems to be the most critical factor regulating the structure of fish larvae community in the study area. The discriminant analysis using the environmenta1 variables (temperature, salinity, and dissolved oxygen) produced result with a difference only of 11.6% from that of c1uster analysis using the taxa composition and abundance data. Consequently, the structural type of fish larvae community can be used for predicting the changes of environmental conditions in the study area.

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An Experimental Study on the Transient Response of Hydrogen Sensors Dependent on Gas Temperature and Humidity (가스의 온도 및 습도 변화에 따른 수소 센서 응답 특성에 대한 실험적 연구)

  • Kim, Young-Doo;Chung, Tae-Yong;Shin, Dong-Hoon;Nam, Jin-Hyun;Kim, Young-Gyu;Lee, Jung-Woon
    • Journal of the Korean Institute of Gas
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    • v.13 no.5
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    • pp.15-19
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    • 2009
  • In this study, the transient responses of hydrogen sensor against abrupt hydrogen release was experimentally studied for three most common types of hydrogen sensors, i.e. the semiconductor type, electrochemical type, and catalytic combustion type. The experimental study was conducted using a 1% hydrogen - 99% nitrogen mixture gas as the standard gas, while the temperature and relative humidity (RH) of the mixture gas was varied from $25^{\circ}C$ to $50^{\circ}C$ and from 50% to 100%, respectively. The temperature of the mixture gas was found to influence the output signal levels of hydrogen sensors, especially the catalytic combustion type. However, the effect of RH on the sensor response was not noticeable in the present experimental study. Thus, the signal levels of hydrogen sensors, in case of catalytic gas sensor need to be calibrated dependent on gas temperature, when the accurate determination of hydrogen concentration is important.

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A Calorimetric Study on the Martensitic Transformation Characteristics with Chemical Composition and Thermal Cycling in Cu-Zr Binary Alloys (Cu-Zr이원계 합금에서 화학조성 및 열싸이클링에 따른 마르텐사이트변태 특성의 열분석학적 연구)

  • Jang, W.Y.;van Humbeeck, J.;Jo, M.S.;Lee, J.H.;Lee, Y.S.;Kang, J.W.;Gwak, S.H.
    • Journal of the Korean Society for Heat Treatment
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    • v.11 no.2
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    • pp.111-120
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    • 1998
  • The effects of chemical composition and thermal cycling on the martensitic transformation characteristics in Cu-rich, equiatomic and Zr-rich CuZr binary alloys have been studied by calorimetry. Only martensite could be indentified in equiatomic $Cu_{49.9}Zr_{50.1}$ alloy, while $Cu_{10}Zr_7$ and $CuZr_2$ intermetallic compounds as well as martensite were formed by rapid cooling from the melts in Cu-rich $Cu_{52.2}Zr_{47.5}$ alloy and Zr-rich $Cu_{48.4}Zr_{51.6}$ alloy, respectively. The $M_s$ temperature of $Cu_{49.9}Zr_{50.1}$ was $156^{\circ}C$ but those of $Cu_{52.5}Zr_{47.5}$ and $Cu_{48.4}Zr_{51.6}$ alloys, being $109^{\circ}C$ and $138^{\circ}C$, were lower than that of equiatomic $Cu_{49.9}Zr_{50.1}$ alloy. In all the alloys, the $M_s$ temperature has fallen but the $A_s$ temperature has risen, resulting in widening of the transformation hysteresis with thermal cycling. The anomalous characteristics in the transformation temperature are due to the presence of the intermetallic compounds i.e. $Cu_{10}Zr_7$ and $CuZr_2$ formed by an eutectoid reaction during thermal cycling in the temperature range between $-100^{\circ}C$ < $T_c$ < $400^{\circ}C$.

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The Analysis of Airborne Trimethylamine Using a Headspace (HS)-SPME Method (헤드스페이스-SPME 방법을 이용한 트리메틸아민의 분석방법 연구)

  • Ahn, Ji-Won;Kim, Ki-Hyun
    • Journal of Korean Society for Atmospheric Environment
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    • v.24 no.3
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    • pp.357-366
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    • 2008
  • In this study, the analytical performance of trimethylamine (TMA) were investigated with respect to headspace-solid phase microextraction (HS-SPME) method. In order to induce the elution of aqueous TMA to headspace, NaOH was added as a decomposition reagent to aqueous TMA standard. By controlling the combination of three major variables for TMA extraction, the extent of extraction was compared between the two contrasting conditions for each variable (i.e., reaction time (long (L) vs short (S)), exposure temperature (30 vs $50^{\circ}C$), and exposure time (10 vs 30 min)). The results of this comparative analysis showed that the extraction efficiency for all eight types of HS-SPME combinations decreased on the order: L-30-30>L-50-10>L-30-10>L-50-30>S-30-30>S-50-30>S-50-10>S-30-10. The effect of reaction time appeared to exert significant influences on the relative recovery rate of HS-SPME at 90% confidence level. However, the effects of exposure temperature or exposure time were not so significant as reaction time. When the recovery rate of HS-SPME is compared against the direct injection of liquid standard into GC injector, it recorded as 2%. According to this comparative study, the reaction conditions for HS-SPME application can exert significant influences on the analysis of TMA.