• Applied Chemistry for Engineering
• /
• v.20 no.3
• /
• pp.273-278
• /
• 2009

A series of poly[2-(2,6-dimethylpyran-4-ylidene)malononitrile-alt-1,4-bis(dodecyloxy)-2,5-divinylbenzene] (PM-PPV), poly[2-{2,6-Bis-[2-(5-bromothiophen-2-yl)-vinyl]-pyran-4-ylidene}-malononitrile-alt-1,4-bis(dodecyloxy)-2,5-divinylbenzene] (PMT-PPV) and poly[2-[2,6-Bis-(2-{4-[(4-bromophenyl)-phenylamino]-phenyl}-vinyl)-pyran-4-ylidene]-malononitrile-alt-1,4-bis(dodecyloxy)-2,5-divinylbenzene] (PMTPA-PPV) were synthesized by the Heck coupling reaction. The band gap of PM-PPV, PMT-PPV and PMTPA-PPV were 2.18 eV, 1.90 eV and 2.07 eV, respectively. The LUMO energy levels of PM-PPV, PMT-PPV and PMTPA-PPV were 3.65 eV, 3.54 eV and 3.62 eV, respectively and the HOMO energy levels of those were 5.83 eV, 5.61 eV and 5.52 eV, respectively. The photovoltaic devices based on the polymers was fabricated. The efficiency of the solar cells based on PM-PPV, PMT-PPV and PMTPA-PPV were 0.028%, 0.031% and 0.11%, respectively and the open circuit voltage (Voc) was 0.59 V~0.69 V under AM 1.5 G and 1 sun condition ($100mA/cm^2$).

• Journal of Life Science
• /
• v.23 no.8
• /
• pp.998-1003
• /
• 2013

The effects of oral administration of estradiol-$17{\beta}$ (E2) on glass eels (Body weight: $0.16{\pm}0.05g$, Total length: $6.2{\pm}0.9cm$) and young eels (Body weight: $2.6{\pm}0.6g$, Total length: $13.2{\pm}0.6cm$) on gonadal sex and growth were examined, respectively. Glass eels were fed a diet containing E2 at a dose of 10 mg/kg or 25 mg/kg, respectively, for five months. The female ratio significantly increased in all E2-treated groups (10 mg/kg diet group: 70%; 25 mg/kg diet group: 90%) when compared to the control group (10%). Young eels were fed a diet containing E2 at a dose of 25 mg/kg for four months. The female ratio also significantly increased in the E2-treated groups (60%) compared to the control group (20%). The highest female ratio was observed in the stage of glass eels rather than young eels. In all experiments, however, the growth of eels treated with E2 was similar to that of controls to the end of the experiment. Thus, oral administration of E2 could be a good approach to controlling sex differentiation.

• Archives of Pharmacal Research
• /
• v.12 no.3
• /
• pp.229-230
• /
• 1989

The reaction of (E)-2, 4-pentadienoic acid with aqueous bromine was reinvestigated to affirm the formation of (E)-5-bromo-4-hydroxy-2-pentenoic acid, whose structure was confirmed by the spectroscopic methods as well as the chemical modification.

• Korean Journal of Veterinary Research
• /
• v.44 no.2
• /
• pp.251-257
• /
• 2004

This study was designed to determine optimal condition for expression of cloned Shigatoxin2e(Stx2e) gene from transformed E. coli PED18, to compare the cytotoxicity titer between cloned Stx2e and Stx2e from original strain, and to confirm of receptor binding affinity of Stx2e for use of development of receptor binding ELISA to detect of Stx2e. The optimum composition of medium for expression of Stx2e gene in E.coli host-vector system was definded as the medium containing 0.5% glucose and 0.5 mM IPTG. The cytotoxicity titer of expressed Stx2e for Vero cell was 1000 fold higher than that of Stx2e from original strain AY93258. The binding affinity of Stx2e to receptor globotetraosyl ceramide($Gb_4$) was confirmed by immunobloting.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.29 no.2
• /
• pp.207-213
• /
• 2014

The structural proteins of classical swine fever virus (CSFV) consist of nucleocapsid protein C and envelope glycoprotein $E^{rns}$ (E0), E1 and E2. Among them, E2, the most immunogenic of the CSFV glycoproteins, induces a protective immune response in swine. In this study, to determine the optimal expression conditions of glycoprotein E2 using baculovirus system, we investigated the influence of insect cells and media to the expression of recombinant E2. Recombinant virus containing glycoprotein E2 coding gene was constructed with bApGOZA DNA. Expression of the glycoprotein E2 was analyzed by SDS-PAGE and Western blot analysis using anti-CSFV E2 monoclonal antibodies. Expression of glycoprotein E2 in Sf21 cells was first observed after 3 days and reached a maximum on the 5th day after infection. Furthermore, the highest levels of glycoprotein E2 expression were observed at multiplicity of infection (MOI) of 5. When three different insect cell lines (Sf21, High-Five and Se301) were tested, High-Five cells showed the highest production. In addition, four different serum-free and serum-supplemented media, respectively, were tested for the expression of glycoprotein E2 and the budded virus (BV) titers. As a result, serum-supplemented medium provided the best conditions for protein production and the BV yield.

• Molecules and Cells
• /
• v.20 no.3
• /
• pp.378-384
• /
• 2005

Estrogen metabolites are carcinogenic. The comparative mitogenic activities of $17{\beta}$-estradiol (E2) and four metabolites, 2-hydroxyestradiol (2-OHE2), 4-hydroxyestradiol (4-OHE2), $16{\alpha}$-hydroxyestrone ($16{\alpha}$-OHE1) and 2-methoxyestradiol (2-ME), were determined in estrogen receptor(ER)-positive MCF-7 human breast cancer cells. Each of the E2 metabolites caused proliferation of the MCF-7 cells, but only E2 and $16{\alpha}$-OHE1 induced a greater than 20-fold increases in transcripts of the progesterone receptor (PR) gene, a classical ER-mediated gene. This suggests that the mitogenic action of E2 and $16{\alpha}$-OHE1 could result from their effects on gene expression via the ER. E2 metabolites altered the expression of E2-regulated proteins including heat shock proteins (Hsps). $16{\alpha}$-OHE1 and 2-ME as well as E2 increased levels of Hsp56, Hsp60, $Hsp90{\alpha}$ and Hsp110 transcripts, and the patterns of these inductions resembled that of PR. Hsp56 and Hsp60 protein levels were increased by all the E2 metabolites. Levels of the transcripts of 3 E2-upregulated proteins (XTP3-transactivated protein A, protein disulfide isomerase-associated 4 protein and stathmin 1) and an E2-downregulated protein (aminoacylase 1) were also affected by the E2 metabolites. These results suggest that the altered expression of Hsps (especially Hsp56 and Hsp60) by E2 metabolites such as E2, $16{\alpha}$-OHE1 and 2-ME could be closely linked to their mitogenic action.

• Proceedings of the Korea Contents Association Conference
• /
• 2006.11a
• /
• pp.333-336
• /
• 2006

E-marketplace is a kind of B2B e-Business system that supports business transactions among companies. If e-marketplace is revitalized, we expect not only the development of related industry but also decrease of transaction cost among companies. It is necessary for the introduction and revitalization of e-marketplace in tourist industry from this point of view. Participants of tour e-marketplace are tour-related companies(travel agencies, lodging enterprises, shipping enterprises, etc.). Also tourists want to search a variety of tour products or contents. So tour e-marketplace has characteristics of B2C e-Business systems as well as B2B e-Business systems at once. The purpose of this study is to classify success factors that determine characteristics of tour e-marketplace through statistics survey from e-marketplace factors related tourism websites. First of all, we analyze success factors of B2B and B2C e-marketplace. Then we will set up influence factors of tour e-marketplace and conduct a survey about success factors of tour e-marketplace. Therefore, we could expect to find these good attributes in tour e-marketplace success through logistic regression and decision tree analysis from source data.

• Journal of Internet Computing and Services
• /
• v.4 no.5
• /
• pp.11-19
• /
• 2003

Since B2C(Business to Customer) from which e-commerce had originated was replaced by B2B, e-Business has shown fast growth so fa., Recently, e-Procurement by l:n concept is on the development as self-purchase system associated with their own ERP In many conglomerates in the Korean market. However, in order to vitalize e-Biz in the plant industry, we need to set up e-marketplaces where many sellers and buyers can meet each other at the same time, which has become the essential part for success as an expanded business model. In this paper, we expect that the foundation for e-transformation in the plant industry is set up by developing Purchase Process System and related modules as the prerequisite for e-Biz in the plant industry, and this report will provide an exemplary model for e-commerce. The Purchase Process System consists of 1) e-Purchasing Module that manages bidding and contract information based on quotation inquiry, 2) e-Expediting Module that manages information to guarantee the on-time delivery, 3) e-Certification Module that controls user authentification, 4) e-Basic Module that manages the bulletin boards, Q&A, etc.

• Journal of the Korean Chemical Society
• /
• v.45 no.4
• /
• pp.312-317
• /
• 2001

7'-Aldehyde-nucleosides analogue (2a, 2c) were synthesized from 6-N-benzoyl-2',3'-O-isopropylideneadenosine and uridine. The condensation of 2 with ethoxycarbonylmethylene Wittig reagent produced the adenosine and uridine analogues containing extended conjugated diene and ethoxycarbonyl group, ethyl-1',5',6',7',8'-pentadeoxy-1'-(adenin-9-yl)-$\beta$-D-ribo-nona-5'(E),7'(E)-dienofuranuronate (4b) and ethyl-1'. 5',6',7',8'-pentadeoxy-1'-(uracil-1-yl)-$\beta$-D-ribo-nona-5'(E),7'-(E)-dienofuranuronate (4c). 9-[8',8'-dibromo-5',6',7',8' -tetradeoxy-$\beta$-D-ribo-octa-5'(E),7'(E)-diene]nucleosides (6b, 6c) were also prepared from 2 with similar method.

• Fashion & Textile Research Journal
• /
• v.21 no.2
• /
• pp.171-178
• /
• 2019

This study analyzed the pressure, subjective wearing comfort, and design preference of brassiere shoulder straps among women in their 20s and 40s. Experimental clothing was made by different shoulder strap designs on a brassiere. In the four designs (A-D), the front attachment points of the shoulder straps were the same, but the back attachment points were different. Three of them (E, E1, E2) were of a halter neck design, with different front attachment positions. The pressures of A-D were measured at the position passing through the shoulder line (P1), and E-E2 was measured at the back neck (P2) as well as at P1. Subjective wearing comfort and design preference were rated on a 7-point Likert scale. A was determined to exert the highest pressure at the neck side of the design in the pressure measurement analysis of A-E2, whereas E1 exerted the lowest. E was measured to have the highest pressure among the E-E2 designs, whereas E2 had the lowest. Participants preferred A the most and E the least in regards to the subjective wearing comfort of A-E. Thus, participants deem A to be the most comfortable despite the high strap pressure. However, E was the most favored design, despite its poor wearing comfort. Participants' subjective impressions of E-E2 were that E2 was the most uncomfortable, but its design was the most preferred. Consumers' design preferences and subjective impressions of wearing comfort did not match.