• YAKHAK HOEJI
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• v.45 no.4
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• pp.339-346
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• 2001

A new oxygenated monoterpene (4) was isolated from the methanol extract of the aerial part of Erechtites hieracifolia together with six known components, a dimethylheptane (1), three ionone derivatives (2, 3 and 7) and two phenylpropanoids (5 and 6). Their structures were identified by means of physico-chemical and spectral data to be (2E, 5E)-6-hydroxy-2,6-dimethylhepta-2,4-dienal (1), 3(R)-hydroxy-5,6-epoxy-$\beta$-ionone (2), 3(R)-hydroxy-5,6-epoxy-7-ionol (3), (3E, 6E)-3,7-dimethylocta-3,5-dien-1,2,7-triol(4), 2-hydroxy-4-(2-propenyl)phenyl-$\beta$-D-glucopyranoside (5), 2-methoxy-4-(2-propenyl)phenyl -$\beta$-D-glucopyra-noside (6) and (6R, 9R)-3-oxo-$\beta$-ionol-$\alpha$-D -glucopyranoside (7).

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.5
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• pp.1936-1942
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• 2010

To examine the effect of overdosed functional food in liver-injured patients, we tried to investigate the dose effect of Allium sativum L. (also called garlic) extract on rats with CCl4-induced liver damage, by comparatively examinating the hepatic function of each group. 42 male Sprague-Dawley rats were selected and divided equally (n=7) into normal, control, positive control, and three experimental subgroups (E1, E2 and E3), respectively. For each kg of body weight, hepatic injury was induced by intraperitoneal administration of $0.5\;m{\ell}$ (0.20 g/kg/day) $CCl_4$, which was diluted in equal amount of olive oil, once a day on every other day for total 5 times. Hot water extraction was performed using domestically cultivated organic garlics, and the obtained extract was injected by sonde, once daily during 4 weeks, to each experimental subgroups by different dosage of 0.35 g/kg(E1), 0.70 g/kg(E2), 1.40 g/kg(E3), respectively. Results showed that the injection of garlic extract positively influenced the physiological activation which lowered the oxidative stress, changed the toxicity, and functionally improved the hepatic condition. Comparing the dose effect between the three experimental subgroups (E1, E2, E3), results of the maximal-dosed subgroup (E3) showed less significance compared with the lower-dosed subgroups(E1 and E2), which seems to resulted from the (increased) toxicity of garlic.

• Journal of Microbiology and Biotechnology
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• v.22 no.2
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• pp.176-183
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• 2012

Eukaryotic translation termination is governed by eRF1 and eRF3. eRF1 recognizes the stop codons and then hydrolyzes peptidyl-tRNA. eRF3, which facilitates the termination process, belongs to the GTPase superfamily. In this study, the effect of the MC domain of eRF1a (eRF1aMC) on the GTPase activity of eRF3 was analyzed using fluorescence spectra and high-performance liquid chromatography. The results indicated eRF1aMC promotes the GTPase activity of eRF3, which is similar to the role of eRF1a. Furthermore, the increased affinity of eRF3 for GTP induced by eRF1aMC was dependent on the concentration of $Mg^{2+}$. Changes in the secondary structure of eRF3C after binding GTP/GDP were detected by CD spectroscopy. The results revealed changes of conformation during formation of the eRF3C GTP complex that were detected in the presence of eRF1a or eRF1aMC. The conformations of the eRF3C eRF1a GTP and eRF3C eRF1aMC GTP complexes were further altered upon the addition of $Mg^{2+}$. By contrast, there was no change in the conformation of GTP bound to free eRF3C or the eRF3C eRF1aN complex. These results suggest that alterations in the conformation of GTP bound to eRF3 is dependent on eRF1a and $Mg^{2+}$, whereas the MC domain of eRF1a is responsible for the change in the conformation of GTP bound to eRF3 in Euplotes octocarinatus.

• Journal of Life Science
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• v.24 no.2
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• pp.173-180
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• 2014

Estrogen can promote or inhibit cellular proliferation depending on tissue cell types and physiological condition and acts through the signal transduction pathways mediated primarily by estrogen receptors. This study examined the effects of fulvestrant (Ful), a well-known antagonist for the estrogen receptor, on the action of $17{\beta}$-estradiol (E2) with respect to the proliferation and apoptosis of Chinese hamster ovarian (CHO) cells. We used different concentrations of E2, Ful, and E2 plus Ful during different treatment durations. Treatment with 15-40 ${\mu}M$ E2 significantly inhibited proliferation in a time-dependent manner, although it had no influence in concentrations up to 1 ${\mu}M$. Interestingly, Ful at 10-40 ${\mu}M$ also inhibited cellular proliferation in both a concentration- and time-dependent manner. In addition, Ful enhanced rather than decreased the inhibitory effect on cellular proliferation by E2 in combined treatment for 10 days. Thus, Ful does not appear to have an antagonistic effect on estrogen's anti-proliferative action in CHO cells. In TUNEL assays to confirm DNA fragmentation by E2 and/or Ful, CHO cells treated with 20 ${\mu}M$ E2 showed a TUNEL-positive reaction in most DAPI-stained nuclei, and cells treated with either 40 ${\mu}M$ Ful or 40 ${\mu}M$ Ful plus 20 ${\mu}M$ E2 also exhibited a TUNEL-positive reaction but at a lower rate compared to the E2-treated cells. These results indicate that Ful does not have an antagonistic effect on estrogen's anti-proliferative action in CHO cells, suggesting that the anti-proliferative and apoptosis-related mechanism(s) through DNA fragmentation by E2 and Ful may be mediated by different signal transduction pathways.

• Proceedings of the Korea Database Society Conference
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• 2001.11a
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• pp.473-505
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• 2001

예측 기관에 따라서 B2B의 시장 규모 및 성장률에 대한 차이는 있지만 B2B 시장이 빠른 속도로 성장하고 있으며, 이 같은 추세는 계속될 것이라는 점에 대한 이견은 없는 편이다. B2B는 기업에게 비용 절감과 시간 절약, 업무 효율성 증대 등의 다양한 효과를 제공해 줄 수 있기 때문에 앞으로도 그 중요성은 더 커질 것으로 예상된다. 그러나 B2B의 중요성 및 성장세와는 별도로 아직까지 B2B에 참여하는 기업들이 큰 효과를 거두지 못하고 있는 것이 사실이다. 이에 따라 많은 학자들과 컨설팅 회사들이 B2B의 모형, 추진 전략, 성공 요인들을 다양한 각도에서 제시하고 있다. 하지만 B2B에 대한 실증적 연구가 부족하여, 기업의 실무자들이 실질적인 도움을 얻기에는 부족한 점이 있기 때문에 B2B의 성공 요인과 추진 전략에 대한 실증적 연구가 절실히 필요하다. 본 연구의 목적은 B2B 유형 중 가장 널리 활용되고 있는 MRO e-marketplace의 성공에 영향을 주는 요인들을 실증적으로 분석하는 것이다. MRO e-marketplace의 성공 요인을 환경적 특성, 제품 특성, B2B 사이트 특성 등 3 그룹으로 분류한 후, 38개 기업에서 수집된 설문지를 분석하여 MRO e-marketplace의 성공 요인을 실증적으로 분석하였다. MRO e-marketplace의 성공 요인들을 요인 분석한 결과, 기업 내부 환경 요인, 기업 외부 환경 요인, 제품 정보 요인, 제품 공급 능력 요인, 사이트 기본 기능 요인, 사이트 편의성 요인, 사이트 보안성 요인 등 총 8개 요인으로 분류되었다. 한편 MRO e-marketplace의 도입 효과를 측정한 비용 절감, 시간 절약, 업무 효율성 증대, 거래 투명성 증대 등의 4개의 문항은 하나의 요인으로 묶여, 이를 MRO e-marketplace 성공으로 정의하였다. MRO e-marketplace의 성공에 영향을 미치는 요인을 찾기 위해, 추출된 8개 요인과 MRO e-marketplace 성공 간의 상관 관계를 분석하였다. 8개 요인 중에서 기업 내부 환경 요인, 제품 공급 능력 요인, 사이트 기본 기능 요인이 MRO e-marketplace의 성공에 영향을 미치는 것으로 나타났다. 마지막으로 MRO e-marketplace 성공 요인들의 상대적 중요도를 파악하기 위해 회귀 분석을 실시하였는데, 참여 기업의 내부 환경 요인이 가장 큰 중요한 것으로 나타났고, 그 다음은 제품 공급 능력 요인과 사이트 기본 기능 요인으로 나타났다. 이 같은 실증적 결과는 MRO e-marketplace나 B2B의 성공을 위해서는 참여 기업의 내부 환경 조성이 매우 중요함을 시사해 준다. 또한 참여 기업의 제품 공급 능력 요인 역시 MRO e-marketplace의 성공에 직접적인 영향을 주기 때문에 공급기업들의 제품 공급 능력을 높이는데 노력해야 한다. 또한 MRO e-marketplace를 운영하는 기업들은 사이트의 기능을 높이는데 많은 노력을 기울여야 한다는 것을 시사하고 있다. MRO e-marketplace의 성공 요인을 실증적으로 분석한 본 연구의 결과는 MRO e-marketplace와 B2B의 추진 전략의 이론적 모형 개발에 유용하게 활용될 수 있을 것이다. 또한 본 연구의 결과는 MRO e-marketplace와 B2B의 성공을 높이기 위한 추진 전략을 수립하는데 유용하게 활용될 수 있을 것으로 기대된다.

• Proceedings of the Korean Vacuum Society Conference
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• 2010.02a
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• pp.161-161
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• 2010

InAsSb alloy system 은 HgCdTe 를 대체하는 적외선 광소자 및 검출기 등에 응용이 가능한 유망한 물질이지만 정확한 유전함수 및 전이점의 연구는 미흡한 실정이다. 본 연구에서는 타원 편광 분석법을 이용하여 1.5 ~ 6 eV 의 분광 영역에서 As 조성비를 각기 (x = 0, 0.127, 0.337, 0.491, 0.726 및 1.00) 다르게 한 $InAs_xSb_{1-x}$ alloy의 유전함수를 측정하였다. 또한 표면에 자연산화막을 제거하기 위하여 Methanol 과 DI Water 로 표면을 세척 한 후 $NH_4OH$, Br in Methanol, HCl 등으로 적절한 화학적 에칭을 하여 산화막을 제거함으로서 순수한 InAsSb 의 유전함수를 측정할 수 있었다. 측정된 InAsSb 유전함수를 Standard analytic critical point line shape 방법으로 As 조성비에 따른 에너지 전이점을 얻을 수 있었다. 또한 얻어진 에너지 전이점 값을 이용하여 linear augmented Slater-type orbital 방법으로 전자 밴드 구조 계산을 하였고, 이를 바탕으로 $E_0$, $E_1$, $E_2$ 전이점 지역의 여러 전이점 ($E_1$, $E_1+\Delta_1$, $E_0'$, $E_0'+\Delta_0'$, $E_2$, $E_2+\Delta_2$, $E_2'$, $E_2'+\Delta_2$, $E_1'$) 의 특성을 정확히 정의할 수 있었다. 또한 As 조성비가 증가하면서 $E_2$, $E_2+\Delta_2$, $E_2'$, $E_2'+\Delta_2$ 전이점들이 서로 교차 되는 것을 발견하였고, 저온에서만 관측이 가능하였던 InSb 의 두 saddle-point (${\Delta_5}^{cu}-{\Delta_5}^{vu}$, ${\Delta_5}^{cl}-{\Delta_5}^{vu}$)를 상온에서 찾아내었다. 타원 편광 분석법을 이용한 전이점 연구 및 물성 분석은 InAsSb alloy 의 광학적 데이터베이스를 확보하는 성과와 더불어 새로운 디바이스기술 및 광통신 산업에도 유용한 정보가 될 것이다.

• Journal of Aquaculture
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• v.11 no.2
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• pp.241-248
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• 1998

Effects of A23187 on estradiol$-17^{\beta}$-induced vitellogenin (VTG) induction were electrophore-tically examined in primary hepatocyte cultures in rainbow trout. hepatocytes were predultured for 2 days and then estradiol-$17^{\beta}$(E2, $2{\times}10^{-6}$M) and calcium ionophore (A23187, $10^{-7)$~$10^{-5}$ M) were added to the incubation medium. The hepatocytes were cultured for 7 more days. In addition, effects of A23187 on $E_2$-primed VTG production were investigated for 7 days. The addition of A23187 ($10^{-7)$~$10^{-5}$M) to the incubation medium specifically reduced VTG production by hepatocytes in a concentration-dependent way. The addition of A23187 significantly reduced the rate of $E_2$-primed VTG production to 18% of the control (E2 only) on Day 7. However, $E_2$-primed VTG production was reduced to 47% of the control by withdrawal of $E_2$ from the incubation medium. Therefore, these results suggest that intracellualr sequestered calcium could regulate VTG synthesis at the translational and/or post-translational stage.

• Archives of Pharmacal Research
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• v.10 no.1
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• pp.50-59
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• 1987

(E)-2-Phenylcyclopropylamine ((E)-TCP), (Z)-2-Phenylacyclopropylamine ((Z)-TCP), (E)-1-methyl-2-phenylcyclopropylamine ((E)-MTCP), and (Z)-1-methyl-2-phenylcyclopropylamine ((Z)-MTCP) were synthesized and used to determine to what extent 1-methylsubstitution and stereochemistry of 2-phenycyclopropylamines affect inhibition of monoamine oxidase (MAO). Inhibition of rat brain mitochondrial MAO-A and B by the compounds were measured using serotonin and benzylamine as the substrate, respectively and $IC_{50}$ values obtianed with 95% confidence limits by the method of computation. For the inhibition of MAO-A, (E)-MTPC ($IC_{50}$ = 6.2 * $10^{-8}$M) was found to be 37 times more potent than (Z)-MTCP ($IC_{50}$ = 7.8 * $10^{-8}$M), was 7 times more potent than (Z)-MTCP($IC_{50}$= 4.7 * $10^{-7}$M) and (E)-TCP($IC_{50}$ =7.8 * $10^{-8}$M),0.6 times as potent as (Z)- TCP ($IC_{50}$ = 4.4 * $10^{-8}$M). The results suggested that while without 1-methyl group, potency of a (Z)-isomer was comparable to that of (E)-isomer, the methyl group in its (Z)-position was very unfavorable to the inhibition of MAO and that in its (E)-position, the methyl group contributed positively to the potency as found by the fact that (E)-MTCP was 1-5 times more potent than (E)-TCP. In view of the selective inhibition of MAO-A- or B over MAO-A and 1-methyl substitution as well as the stereochemical factors did not significantly influence the selectivity.

• Food Science and Preservation
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• v.7 no.4
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• pp.366-372
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• 2000

Volatile flavor components in three grape(Vitis vinifera L.) varieties were extracted by SDE(Simultaneous steam distillation and extraction) method using the mixture of n-pentane and diethylether(1:1, v/v) as an extract solvent. Grapes of the following varieties were studies : Blackolympia, Campbell and Delaware. The volatile extracts were analyzed by GC-FID and GC/MS. The totals of 77, 72 and 74 volatile flavor components were identified in Blackolympia, Campbell and Delaware, respectively. (E)-2-Hexenal(20.36%), diethylacetal(18.03%), hexanal and ethyl acetate were contained as the main compounds of Blackolympia. In Campbell, ethyl acetate(30.81%) was relatively more abundant than other compounds and among functional groups, C$\_$6/ aldehydes and alcohols were major constituents of the extract. On the other hand, in Delaware, alcohols was the major constituent group and (E)-2-hexenal(21.07%) and (E)-2-hexena1-ol(19.43%) were the main compounds. All of three grape varieties contained a large amount of hexanal, (E)-2-hexenal, hexanol, (E)-2-hexen-1-ol, thus C$\_$6/-compounds proved to be major volatile components of grape and small amount of terpenols were only detected from Delaware.

• Journal of Korean Ophthalmic Optics Society
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• v.9 no.2
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• pp.323-332
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• 2004

This study researched the visual acuity test object and Auto-refractormeter, visual of near power. The object were composed of middle aged, the old men and women who in habit Daegu. The results were as follows : 1. The subjects consisted of 537 people, 29.98% men, 70.02% women. 2. The emmetropia was 1.12% for myopia, 2.79% for hyperopia, 96.09% for astigmatism. 3. The abnormal refraction was composition for myopic compound astigmatism(16.57%), hyperopia compound astigmatism(45.62%), Mixed astigmatism(33.89%). 4. On the Myopic Spherical Equivalent(S.E) power, the range of -0.50D ${\leq}$ M.S.E < -1.00D was 21.67%, -1.00D ${\leq}$ M.S.E < -2.00D was 48.89%, -2.00D ${\leq}$ M.S.E < -6.00D was 29.44%. 5. On the Hyperopic Spherical Equivalent(S.E) power, the range of +0.50D ${\leq}$ H.S.E < +1.00D was 28.57%, +1.00D ${\leq}$ H.S.E < +2.00D was 49.30%, +2.00D ${\leq}$ H.S.E < +6.00D was 23.13%. 6. The addition power was 1.00D(8.01%), 1.50D(8.57%), 2.00D(13.78%), 2.50D(16.57%), 3.00D(16.95%), 3.50D(17.88%), 4.00D(18.25%).