Browse > Article
http://dx.doi.org/10.5762/KAIS.2010.11.5.1936

The effect of Allium sativum L. extract on hepatic function in rats with CCl4-induced (hepatic) injury  

Lee, Tae-Jong (Department of Pathology, Daegu Fatima Hospital)
Min, Kyung-Jin (Department of Public Health, Keimyung University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.11, no.5, 2010 , pp. 1936-1942 More about this Journal
Abstract
To examine the effect of overdosed functional food in liver-injured patients, we tried to investigate the dose effect of Allium sativum L. (also called garlic) extract on rats with CCl4-induced liver damage, by comparatively examinating the hepatic function of each group. 42 male Sprague-Dawley rats were selected and divided equally (n=7) into normal, control, positive control, and three experimental subgroups (E1, E2 and E3), respectively. For each kg of body weight, hepatic injury was induced by intraperitoneal administration of $0.5\;m{\ell}$ (0.20 g/kg/day) $CCl_4$, which was diluted in equal amount of olive oil, once a day on every other day for total 5 times. Hot water extraction was performed using domestically cultivated organic garlics, and the obtained extract was injected by sonde, once daily during 4 weeks, to each experimental subgroups by different dosage of 0.35 g/kg(E1), 0.70 g/kg(E2), 1.40 g/kg(E3), respectively. Results showed that the injection of garlic extract positively influenced the physiological activation which lowered the oxidative stress, changed the toxicity, and functionally improved the hepatic condition. Comparing the dose effect between the three experimental subgroups (E1, E2, E3), results of the maximal-dosed subgroup (E3) showed less significance compared with the lower-dosed subgroups(E1 and E2), which seems to resulted from the (increased) toxicity of garlic.
Keywords
garlic; Liver-Damaged; Liver Function; $CCl_4$;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Reddy BM, Venkatesh SGD, Reddy M, Ramesh AVA, Appa Rao. "Antihyperglycemic activity of Caralluma attenuata." J Fitotera, Vol 74, pp.247-279, 2009.
2 Hu JJ, Lim M, Wang EJ, Yang CS. "Protective effects of diallyl sulfide on acetaminophen-induced toxicities."Chemical Toxico, Vol 34, pp.963-969, 2006.
3 Vimal V, Devaki T. "Hepatoprotective effect of allicin on tissue defensesystem in galactosamine/endotoxin challenged rats." J Ethnopharmacolo, Vol 90, pp.151-154, 2004.   DOI
4 Louria DB, Bogden JD. "The dangers from limited exposure to carbon tetrachloride." Critical Reviews of Toxicol, Vol 7, pp.177-188, 2006.
5 Salmi HA, Sarna S. "Effect of silymarin on chemical, functional, and morphological alterations of the liver." J Gastroenterol, Vol 17, pp.517-521, 2006.
6 Ferenci P, Dragosics B, Dittrich H, Benda H, Lochs L, Base S, Scheider W. "Randomized controlled trial of silymarin treatment in patients whit cirrhosis of the liver." J Patholo, Vol 9, pp.105-113, 2005.
7 McGrowan M, Artiss DR. "A peroxidase-coupled method for the colorimetric determination of serum triglycerides." Clin. Chem, Vol 29,pp 538-542, 1983.
8 AL-Bekairi AM, Shah AH, Qureshi S. "Effect of Allium sativum on epididymal spermatozoa, estradiol-treated mice and general toxicity." J Ethnopharmacol, Vol 29, pp.117-125, 1990.
9 Sheela CG, Augusti KT. "Antidiabetic effects of S-allylcysteine sulphoxide isolated from garlic Allium sativum Linn." J Experimental, Vol 30, pp.523-526, 1992.
10 Sheo HJ. "Toxic effects of the megadose garlic juice in the rats." J Food Science, Vol 29, pp.485-492, 2000.
11 Ahmed RS, Sharma SB. "Biochemical studies on combined effects of garlic (Allium sativum Linn) and ginger (Zingiber officinale Rosc) in albino rats." Indi J Exp Biolo, Vol 35, pp.841-843, 2007.
12 Lee SY. "Comparative study of the allicin and arsenite on albino rats with special regard to the effects on body weight, hemoglobin and hepatic histology." J Medic, Vol 10, pp.99-103, 1967.   DOI
13 Jain RC. "Onion and garlic in experimental Atherosclerosis." Lancet, Vol 31, pp.256-257, 1995.
14 Gedik N, Kabasakal L, Sehirli O, Ercan F, Sirvanci S, Keyer-Uysal M, Sener G. "Long-term administration of aqueous garlic extract(AGE) alleviates liver fibrosis and oxidative damage induced by biliary obstruction in rats." Life Scien, Vol 76, pp.2593-2606, 2005.   DOI
15 Vimal V, Devaki T. "Hepatoprotective effect of allicin on tissue defensesystem in galactosamine/endotoxin challenged rats." J Ethnopharmacol, Vol 90, pp.151-154, 2004.   DOI
16 Ilker D, Mustafa K, Bilal A, Aslihan A. "Effects of garlic extract consumption on blood lipid and oxidant/antioxidant parameters in humans with high blood cholesterol." J Nutritional Biochem, Vol 15, pp.373-377, 2004.   DOI