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Isolation and Identification of Yeasts from Wild Flowers Collected around Jangseong Lake in Jeollanam-do, Republic of Korea, and Characterization of the Unrecorded Yeast Bullera coprosmaensis

  • Han, Sang-Min;Hyun, Se-Hee;Lee, Hyang Burm;Lee, Hye Won;Kim, Ha-Kun;Lee, Jong-Soo
    • Mycobiology
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    • v.43 no.3
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    • pp.266-271
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    • 2015
  • Several types of yeasts were isolated from wild flowers around Jangseong Lake in Jeollanam-do, Republic of Korea and identified by comparing the nucleotide sequences of the PCR amplicons for the D1/D2 variable domain of the 26S ribosomal DNA using Basic Local Alignment Search Tool (BLAST) analysis. In total, 60 strains from 18 species were isolated, and Pseudozyma spp. (27 strains), which included Pseudozyma rugulosa (7 strains) and Pseudozyma aphidis (6 strains), was dominant species. Among the 60 strains, Bullera coprosmaensis JS00600 represented a newly recorded yeast strain in Korea, and its microbiological characteristics were investigated. The yeast cell has an oval-shaped morphology measuring $1.4{\times}1.7{\mu}m$ in size. Bullera coprosmaensis JS00600 is an asporous yeast that exhibits no pseudomycelium formation. It grew well in vitamin-free medium as well as in yeast extract-malt extract broth and yeast extract-peptone-dextrose (YPD) broth, and it is halotolerant growing in 10% NaCl-containing YPD broth.

A Study on Arguments over the Light Pink Color of Official Uniforms in the Joseon Dynasty (Part I) (조선시대 관복 담홍포 담론 연구 (제1보))

  • Park, Hyun-Jung
    • Journal of the Korean Society of Clothing and Textiles
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    • v.35 no.9
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    • pp.1125-1137
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    • 2011
  • This study investigates when and why the light pink Dan-ryoung appeared as part of the official uniform of the Joseon Dynasty and which official uniform used the light pink color. The Annals of the Joseon Dynasty, books written by scholars, and related laws were used as research materials. The following results were obtained by analyzing the arguments of kings and officials on light pink uniforms and laws related to official uniforms. 1. Red was the color of the king as well as the color of Dang-sang-gwan's Gong-bok (one of the official uniforms). 2. The colors such as To-hong, Dam-hong, Do-hong, Bun-hong, Cheon-hong that appeared in Sang-bok (among official uniforms) were relatively lighter than red. 3. To-hong started to be used in Sang-bok at the time of King Se-jong and was used at the time of King Seong-jong in Sang-bok because it was the preferred color. 4. In the Joseon Dynasty, safflower (the basis of red color dye) was cultivated extensively; in addition, people liked red dyeing because it was relatively easy apply; subsequently, a ban on red dyeing was continually issued. 5. Kings Se-jong and Jung-jong ordered officials to use Do-hong and Bun-hong to distinguish the red color of the king. After Im-jin-oe-ran, Cheon-hong was officially designated the color of Sang-bok. 6. The reasons why Dam-hong was used in official uniforms were twofold: the preference for red-like colors and the influence of the Confucian hierarchy to distinguish the king.

Compaction Properties of Fe Powder Fabricated by Warm Compaction (온간성형법으로 제조된 Fe 분말의 성형특성)

  • Kim, Se-Hoon;Lee, Young-Jung;Lee, Jea-Sung;Kim, Young-Do
    • Journal of Powder Materials
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    • v.14 no.3 s.62
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    • pp.185-189
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    • 2007
  • Various approaches have been proposed to increase the green density. Warm compaction method has been used for the reduction of residual stress, the improvement of magnetic properties and the higher densities. In this work, the effect of warm compaction on green density of Fe powder was investigated. After ball-milling of Fe oxide powder for 30 hours, Fe oxide powder was reduced through the hydrogen reduction process. The pure Fe powder and polymer binder were mixed by 3-D tubular mixer. And then the mixed powder was warm-compacted with various compaction pressure and binder contents. The green density of specimen was added polyvinyl binder was higher than any other specimens.

COMPATIBILITY OF SELF-ETCHING DENTIN ADHESIVES WITH RESIN LUTING CEMENTS (자가부식형 상아질접착제와 레진시멘트와의 적합성에 관한 연구)

  • Kim, Do-Wan;Park, Sang-Jin;Choi, Kyoung-Kyu
    • Restorative Dentistry and Endodontics
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    • v.30 no.6
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    • pp.493-504
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    • 2005
  • This study was performed to investigate the compatibility between 4 dentin adhesives and 4 resin luting cements. Dentin adhesives used in this study were All-Bond 2 (Bisco Inc., Schaumbrug, IL, USA), Clearfil SE-Bond (Kuraray Medical Inc, Osaka, Japan), Prompt L-Pop (3M Dental Products, St. Paul, MN, USA), One-Up Bond F (Tokuyama corp., Tokyo, Japan) Resin luting cements used in this study were Choice (Bisco Inc., Schaumbrug, IL, USA), Panavia F (Kuraray Medical Inc, Osaka, Japan), RelyX ARC (3M Dental Products, St. Paul, MN, USA) Bistite II DC (Tokuyama corp., Tokyo, Japan). Combination of each dentin adhesive and corresponding resin cement was made to 16 experimental groups. Flat dentin surfaces was created on mid-coronal dentin of extracted mandibular third molars, then dentin surface was polished with 320-grit silicon carbide abrasive papers. Indirect resin composite block (Tescera, Bisco) was fabricated. Its surface for bonding to tooth was polished with silicon carbide abrasive papers Each dentin adhesive was treated on tooth surface and resin composite overlay were luted with each resin cement. Each bonded specimen was poured in epoxy resin and sectioned occluso-gingivally into 1.0mm thick slab, then further sectioned into $1.0{\times}1.0mm^2$ composite-dentin beams. Microtensile bond strength was tested at a crosshead speed of 1.0mm/min. The data were analysed by one-way ANOVA and Duncan's multiple comparison tests The results of this study were as follows, 2-step self-etching dentin adhesive which has additional bonding resin is more comparison than tests. self-etching dentin adhesive.

Characteristics of Fracture System in Precambrian Metamorphic Rocks and Mesozoic Granites from Seokmo-do, Ganghwa-gun (강화군 석모도 일대의 선캠브리아기 변성암류 및 중생대 화강암류에서 발달하는 단열계의 분포특성)

  • Park, Deok-Won;Lee, Chang-Bum
    • The Journal of the Petrological Society of Korea
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    • v.19 no.2
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    • pp.123-139
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    • 2010
  • The properties of fracture system in Precambrian Jangbong schist and Mesozoic granites from Seokmo-do, Ganghwa-gun were investigated and analyzed. Most of the fractures measured at outcrops are nearly vertical or steeply dipping. Orientations of fracture sets in terms of frequency order are as follows: Set $1:N2^{\circ}E/77^{\circ}SE$, Set $2:N17^{\circ}E/84^{\circ}NW$, Set $3:N26^{\circ}E/64^{\circ}SE$, Set $4:N86^{\circ}W/82^{\circ}SW$, Set $5:N80^{\circ}W/77^{\circ}NE$, Set $6:N60^{\circ}W/85^{\circ}SW$, Set $7:N73^{\circ}E/87^{\circ}NW$, Set $8:N82^{\circ}W/53^{\circ}NE$, Set $9:N23^{\circ}W/86^{\circ}SW$, Set 10: $N39^{\circ}W/61^{\circ}NE$. Especially, the rose diagram of fracture strikes(N:240) indicates that there are two dorminant directions of N-S~NNE and WNW. These distribution pattern of fractures from Seokmo-do correponds with those of major lineaments from South Korea suggested in previous study. Meanwhile, the scaling properties on the length distribution of fracture populations have been investigated. First, fracture sets from Precambrian Jangbong schist and Mesozoic granites(north and south rock body) has been classified into five groups(group I~V) based on strike and frequency. Then, the distribution chart generalized the individual length-cumulative frequency diagram for above five groups were made. From the related chart, five subpopulations(group I~V) that closely follow a power-law length distribution show a wide range in exponents(-0.79~-1.53). These relative differences in exponent among five groups emphasizes the importance of orientation effect. From the related chart, the diagram of group III occupies an upper region among five groups. Finally, the distribution chart showing the chracteristics of the length frequency distribution for each rock body were made. From the related chart, the diagram of each rock body shows an order of porphyritic biotite granite < hornblende granodiorite < medium-grained biotite granite(south rock body) < medium-grained biotite granite(north rock body) < Precambrian Jangbong schist. From the related chart, the diagram of more older rock body in the formation age tends to occupy an upper region. Especially, the diagram of Precambrian Jangbong schist occupies an upper region compared with the diagrams of Mesozoic granites. These distributional chracteristics suggests that coexistence of new fracture initiation and growing of existing fractures corresponding with stress field acted since the formation of rock body.

The Absorption and Purification of Air Pollutants and Heavy Metals by Selected Trees in Kwangju (광주지역(光州地域)에서 주요(主要) 수목(樹木)의 대기오염물질(大氣汚染物質)과 중금속(重金屬) 흡수(吸收) 정화기능(淨化機能)에 관(關)한 연구(硏究))

  • Cho, Hi Doo
    • Journal of Korean Society of Forest Science
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    • v.88 no.4
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    • pp.510-522
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    • 1999
  • The air pollutants ; $SO_2$, $SO{_4}^{-2}$, $NO{_3}^-$, $Cl^-$ are absorbed into soils through falling with dusts and rain from the atmosphere. The sources of heavy metal contaminants in the environments are agricultural and horticultural materials, sewage sludges, fossil fuel combustion, metallurgical industries, electronics and waste disposal etc.. The soils and hydrosphere can be polluted on the way of the circulation of these heavy metals. Studied pollutant anions are $SO{_4}^{-2}$, $NO{_3}^-$ and $Cl^-$ and heavy metals are Se, Mo, Zn, Cd, Pb, Mn, Cr, Co, V, As, Cu and Ni which are the elements to be concerned with the essentials for plants, with animal and human health. This study is with the aim of selecting the species of roadside trees and green space trees which have excellent absorption of air pollutants and heavy metals from the atmosphere and the soils in the urban area. Two areas are designated to carry out this study : urban area ; Kwangju city and rural area ; the yard of Forest Environment Institute of Chollanam-do, at Sanje-ri, Sampo-myum, Naju city, Chollanam-do (23km away from Kwangju). This study is carried out to understand the movement of anions and heavy metals from the soils to the trees in both areas, the absorption of anions and heavy metals from atmosphere into leaves and the amounts of anions and heavy metals in leaves and fine roots(< 1mm dia.) of roadside trees and green space trees in Kwangju and trees in the yard of Forest Environment Institute of Chollanam-do. The tree species selected for this study in both areas are Ginkgo biloba, Quercus acutissima, Cedrus deodara, Platanus occidentalis, Robinia pseudoacacia, Alnus japonica. Metasequoia glyptostroboides. Zekova serrata. Prunus serrulata var. spontanea, and Pinus densiflora. The results of the study are as follows : 1. $SO{_4}^{-2}$, $NO{_3}^-$ and $Cl^-$ concentrations are higher in the soils of the urban area than in those of the rural area, and $NO{_3}^-$ and $SO{_4}^{-2}$ are higher in the leaves than in the roots due to the absorption of the these pollutants through the stomata. 2. Ginkgo biloba, Robinia pseudoacacia. Zekova serrata, Quercus acutissima, and Platanus occidentalis can be adequated to the roadside trees and the environmental trees due to their good absorption of $NO{_3}^-$ and $SO{_4}^{-2}$. 3. Heavy metals in the soils of both areas are in the order of Mn > Zn > V > Cr > Pb > Ni > Cu > Mo> Cd, and in the leaves and roots of the trees in the both areas are in the order of Mn>Zn>Cr>Cu>V>Ni. Both orders are similar ones except V. There are more in the urban soils than in the rural soils in amount of Mn, Zn, Pb, V, Cu. 4. It is supposed that there is no antagonism between Mn and Zn in this study. 5. Se, Co and As are not detected in the soils, the leaves and the roots in both areas. Sn, Mo, Cd and Pb are also not detected in the leaves and roots in spite of considerable amount in the soils of both areas.

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Chest compression quality, exercise intensity, and energy expenditure during cardiopulmonary resuscitation using compression-to-ventilation ratios of 15:1 or 30:2 or chest compression only: a randomized, crossover manikin study

  • Kwak, Se-Jung;Kim, Young-Min;Baek, Hee Jin;Kim, Se Hong;Yim, Hyeon Woo
    • Clinical and Experimental Emergency Medicine
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    • v.3 no.3
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    • pp.148-157
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    • 2016
  • Objective Our aim was to compare the compression quality, exercise intensity, and energy expenditure in 5-minute single-rescuer cardiopulmonary resuscitation (CPR) using 15:1 or 30:2 compression-to-ventilation (C:V) ratios or chest compression only (CCO). Methods This was a randomized, crossover manikin study. Medical students were randomized to perform either type of CPR and do the others with intervals of at least 1 day. We measured compression quality, ratings of perceived exertion (RPE) score, heart rate, maximal oxygen uptake, and energy expenditure during CPR. Results Forty-seven students were recruited. Mean compression rates did not differ between the 3 groups. However, the mean percentage of adequate compressions in the CCO group was significantly lower than that of the 15:1 or 30:2 group ($31.2{\pm}30.3%$ vs. $55.1{\pm}37.5%$ vs. $54.0{\pm}36.9%$, respectively; P<0.001) and the difference occurred within the first minute. The RPE score in each minute and heart rate change in the CCO group was significantly higher than those of the C:V ratio groups. There was no significant difference in maximal oxygen uptake between the 3 groups. Energy expenditure in the CCO group was relatively lower than that of the 2 C:V ratio groups. Conclusion CPR using a 15:1 C:V ratio may provide a compression quality and exercise intensity comparable to those obtained using a 30:2 C:V ratio. An earlier decrease in compression quality and increase in RPE and heart rate could be produced by CCO CPR compared with 15:1 or 30:2 C:V ratios with relatively lower oxygen uptake and energy expenditure.

Development of In-Pipe Robot Using Clutch-Based Selective Driving Algorithm (클러치기반의 선택적 구동방식을 이용한 배관로봇의 개발)

  • Kim, Do-Wan;Roh, Se-Gon;Lee, Jung-Sub;Lee, Soo-Hwan;Choi, Hyouk-Ryeol
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.32 no.3
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    • pp.223-231
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    • 2008
  • This paper introduces a robot called the MRINSPECT V (Multifunctional Robotic crawler for Inpipe in-SPECTion V) for the inspection of pipelines with a nominal 8-in inside diameter. Based on the mechanism of the previous model MRINSPECT IV, we developed a new MRINSPECT V by using the differential driving mechanism, so that just simply controlling the speed of each driving units helps the robot to travel effectively inside the pipelines. Furthermore, the robot uses clutches in transmitting driving power to wheels. This clutch mechanism enables MRINSPECT V to select the suitable driving method according to the shape of pipeline. In this paper, the critical points in design and construction of the proposed robot are described with the preliminary results to provide good mobility and increase the efficiency.

A Study on Rural Theme Park Considering the Local Characteristics (지역적 특성을 고려한 농촌테마공원에 관한 연구)

  • Rhee, Shin-Ho;Choi, Se-Hyun;Yoon, Seong-Soo;Choi, Ae-Soon;Eom, Dae-Ho
    • Journal of The Korean Society of Agricultural Engineers
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    • v.53 no.3
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    • pp.27-34
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    • 2011
  • The definition of a rural theme park was presented and it's development directions were set up as grasping the problems of existing rural theme park. The creation method of a rural theme park was presented through the case study of a design planning. A rural theme park should be designed with consideration to regional characteristics based on the rural amenity. The characteristics of rural theme parks set by theme, unity, integrity, originality, and eco-friendly. And the visit reasons of rural theme park are rural experience, lodging, relaxation or recreation, comfort or easy, and food purchase. A case study area is around Geopo-Ri and Gueum-Ri Okcheon-Gun Chungcheongbuk-Do. The master plan of this area was prepared.

User Matching System for Activating Sports Tourism Based on Hybrid App

  • Kim, Se-won;Moon, Seok-Jae;Ryua, Gihwan
    • International Journal of Advanced Culture Technology
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    • v.8 no.3
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    • pp.241-246
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    • 2020
  • In this paper, we propose a user matching app based on hybrid app and a utilization plan to promote sports tourism in line with the growing trend of sports industry scale. The proposed app categorizes sports facilities across the country into regional, sports, private and public sports facilities to support reservations and matching. The proposed app applied a matching system in which matching scores were given according to the preference of events, places, and users by user net matching algorithm. Users can enjoy sports as a team or individual through the suggestion app even if they do not have any clubs or friends to which they belong. It can be used to improve tourism content services and establish tourism industry policies by analyzing data generated while using a user matching system.