• Journal of Plant Biology
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• 제33권4호
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• pp.315-320
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• 1990

We investigated the interaction of auxin and Ca2+ on coleoptile segment elongation in seedlings of Zea mays L. Seedlings imbibed and raised either in the presence of 10 mM CaCl2 (HC), or in the absence of Ca2+ (LC) were used. Exposure to 10-5M auxin of coleoptiles from either HC or LC seedlings resulted in strong promotion of elongation. However, longer latent period (90 min) of the auxin effect was observed in HC than in LC seedlings (20 min). The length of latent period observed in HC coleoptiles was proportional to the concentration of CaCl2. The latent period of auxin effect observed in HC seedlings was abolished by pretreatment of the coleoptiles with TMB-8 which inhibits IP3-induced Ca2+ release from the tonoplasts. In segments of LC seedlings, the promotive effect of IAA (10-5M) was abolished by treatment with 5 mM calcium but was reversible upon treatment of the segments with 5 mM EGTA. These results suggest that the effect of auxin on coleoptile elongation is closely related to intracellular Ca2+ level.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.217-217
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• 2017

Seed priming leading to improved emergences, germination speed and uniformity under unfavorable conditions without loss of longevity are the best level of techniques. We studied the possibility to obtain primed seed with reduced mean germination time (MGT) and uniformity but with simple, fast and effective methods. The experiments were carried out at $15^{\circ}C$ for 16 hours with priming treatment. To compare the effects of each treatment, we used 200mM $CaCl_2$ priming or hydro priming or no imbibition after ultrasonication or red light treatment. The light treatment was performed by irradiating 2000 lux of red light for 15, 30, 60, and 120 minutes. Addition, in order to investigate the effect of the priming solution, treated at 200mM $CaCl_2$ for 8 hours. Ultrasonication treatment was performed for 5, 10, and 20 minutes at exposures of 13.0, 17.3, and 21.6 kHz during priming. For liquid smoke (LS) treatment, seed soaked in 0%, 0.5%, 1.0%, 5.0% and 10.0% of diluted water and 200mM $CaCl_2$ solution. After each treatment, the seeds were dried to moisture content ranged 5-8% at $25^{\circ}C$ for 24 hours. The effect of seed treatment was evaluated with germination percentage (GP), MGT, germination index(GI), germination rate(GR), Germination Uniformity(GU) and heath seed percentage(HS). For several factors tested, we found that the desired germination improvement could be obtained by treating the seed with ultrasonication at 17.3 kHz for 5 minutes in water or red light exposure at 2000 lux for 120 min in water that resulted very similar to those used to 200mM $CaCl_2$ priming for 16 hrs. However, LS treatment showed no improvement in all diluted solution. Therefore, the methods applied ultrasonication and red light treatment showed high potential for fast and easy treatment avoiding pollution of salt solution waste.

• 한국조경학회지
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• 제45권3호
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• pp.1-8
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• 2017

이 연구는 염화칼슘이 스트로브잣나무(Pinus strobus)의 생장 및 생리반응에 미치는 영향과 그 중 염화칼슘에 민감하게 반응하는 변수를 알아보고자 실시되었다. 이를 위해 염화칼슘에 처리에 대한 스트로브잣나무의 가시적 피해, 근원경 생장, 식물체 내 수분함수량, 엽록소함량과 구성, 광계 II의 최대 활성, 전자전달율의 변화를 분석하였다. 염화칼슘 처리는 개엽하기 전에 염화칼슘 0.5, 1.0, 3.0% 수용액을 근권부에 관수하였다. 염화칼슘 처리에 의한 가시적 피해로 잎의 갈변과 탈락, 잎 마름이 나타났으며, 이러한 피해 양상은 염화칼슘 농도가 높고, 처리 기간이 길어질수록 가중되어 나타났다. 근원경(밑둥지름) 생장량 그리고 잎의 수분함수량의 감소는 염화칼슘 1.0%와 3.0% 처리에서 유의하게 나타났다. 광색소인 총엽록소함량, 광계 II의 최대 활성, 전자전달율은 염화칼슘 3.0% 처리에서 대조구 대비 유의한 감소를 보였다. 결론적으로 염화칼슘 처리는 엽 내 수분상태에 영향을 주었고, 광수확 능력과 광화학반응 능력의 감소를 초래하였다. 또한, 염화칼슘 농도와 생장 및 생리반응 변수들 간의 상호 연관성을 분석한 결과, 잎의 수분함수량과 엽록소 a와 b의 비율의 회귀식 결정계수가 상대적으로 높아, 스트로브잣나무의 염화칼슘 피해 수준에 민감하게 반응하는 변수로 나타났다.

• 한국식품저장유통학회지
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• 제13권1호
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• pp.13-17
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• 2006

딸기를 $20^{\circ}C$와 $45^{\circ}C$의 $1\%\;CaCl_2$ 용액에 각각 15분간 침지시킨 후, PE film (0.011mm)으로 포장한 군과 포장하지 않은 군으로 나누어 $5^{\circ}C$에 저장하였다. 그리고 $CaCl_2$, 용액에 침지시키지 않고 PE film(0.011mm)으로 포장한 군과 포장하지 않은 군으로 나누어 $5^{\circ}C$에 저장하였다. $45^{\circ}C\;CaCl_2$ 용액에 침지시킨 후 포장한 처리구에서 부패율은 낮고 경도가 높게 나타났다. 또한 비타민 C함량이 높았고 외관이 가장 좋은 것으로 나타났으며 다른 처리군에 비해 pH가 낮고 산도가 높게 나타났다. 그러나 당도는 처리군 간에 유의성이 없었다. 따라서 수확 후 칼슘 처리와 포장의 병행은 딸기의 저장에 효과적인 것으로 사료된다.

• 한국농림기상학회지
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• 제11권4호
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• pp.143-150
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• 2009

겨울철 도로의 안전을 위해서 사용되고 있는 제설제가 가로변의 식생에 축적이 되면 식물에 피해를 가져올 수 있는데, 특히 이른 봄의 제설제 사용은 식물이 다량의 수분을 요구하게 되는 개엽 시기와 맞물리게 되면서 그 피해가 가속화 될 수 있다. 우리나라에서 주로 사용하고 있는 제설제인 $CaCl_2$ 으로 산딸나무(C.kousa) 3년생 유묘를 대상으로 개엽 전 대조구를 포함해 각 처리구당 3본씩 선정하여 배치하고 각각 $CaCl_2$ 0.5%, 1.0%, 3.0% 를 2회 근권부에 처리하였다. 먼저, 가시적 피해 현상으로 잎눈에서 개엽이 어려워지며, 잎의 전개 후에도 시들음 현상과 잎끝마름 현상 등이 나타났다. $CaCl_2$ 의 농도가 높아짐에 따라 산딸나무의 피해가 가중되었는데, $CaCl_2$ 처리에 따른 생리적 반응은 총엽록소함량, 엽록소 a:b, 광합성속도, 양자수율, 기공전도도, $F_V/F_M$, NPQ의 감소를 가져왔다. 반면 광보상점과 암호흡속도의 증가를 나타냈다. 기공의 닫힘으로 인한 엽육내 $CO_2$ 농도 저하와 이로 인한 광합성속도의 감소가 1차적으로 일어나고 이후 물질대사량의 감소, 광화학계의 피해가 동반되는 것을 알 수 있었다. 모든 $CaCl_2$ 처리농도에서 산딸나무의 생리적 활동이 급격히 저하되는 경향으로 볼 때 $CaCl_2$ 에 민감한 수종으로 판단된다.

• 약학회지
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• 제59권4호
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• pp.177-183
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• 2015

The mechanism of melanogenesis induced by cyclosporin A (CsA) was investigated in B16 melanoma cells. CsA stimulated the production of melanin in a dose-dependent manner in the cells. In addition, CsA increased intracellular $Ca^{2+}$ concentration in a dose-related fashion. Treatment with BAPTA/AM, an intracellular $Ca^{2+}$ chelator significantly inhibited the CsA-induced intracellular melanin synthesis. CsA profoundly induced $Cl^-$ efflux, which was significantly blocked by niflumic acid (NFA) and flufenamic acid (FFA), specific and nonspecific inhibitors of $Ca^{2+}$-activated $Cl^-$ channels (CaCCs), respectively. Furthermore, these inhibitors of CaCCs significantly inhibited the CsA-induced stimulation of melanin synthesis. Taken together, these results suggest that the activation of CaCCs may play an important role in the CsA-induced stimulation of melanin synthesis in B16 cells. These results further suggest that CaCCs may be a good target for the management of hyperpigmentation of the skin reported in the patients treated with CsA.

• Journal of Animal Science and Technology
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• 제64권5호
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• pp.871-884
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• 2022

Two experiments were conducted to evaluate the effects of calcium (Ca) levels in weanling pigs (Landrace × Yorkshire × Duroc). In experiment 1, one hundred and eighty weanling pigs were randomly allotted to one of the three treatments. The treatments were low (Ca 0.60% in phase 1 and 0.50% in phase 2), standard (Ca 0.72% in phase 1 and 0.66% in phase 2), and high (Ca 0.84% in phase 1 and 0.72% in phase 2). In experiment 2, hundred and forty weanling pigs were randomly assigned to one of four treatments differing in Ca levels (high and low) and sources (CaCl₂ and CaCO₃) in a 2 × 2 factorial arrangement. There were 10 pigs per replicate in both experiments, with 6 replicates in each treatment, and they were conducted in two phases (phase 1, days 0-14; phase 2, days 15-28). In experiment 1, body weight (BW), average daily gain (ADG), and growth to feed ratio (G/F) increased as the Ca level decreased (p < 0.05). P digestibility was higher in the low-Ca diet group than in the high-Ca diet group (p <0.05). In experiment 2, the final BW, ADG, and G/F increased in the CaCl₂ diet group compared with the case in the CaCO₃ diet group (p < 0.05). The digestibility of crude protein (CP), Ca, and P was higher in the CaCl₂ diet group than in the CaCO₃ diet group (p < 0.05). Cl^- levels were higher in the CaCl₂ diet group than in the CaCO₃ diet group (p < 0.05). The bicarbonate (HCO_3-), base excess (BE), and electrolyte balance (EB) levels were lower in the CaCl₂ diet group than in the CaCO₃ diet group (p < 0.05). Hematocrit increased as the Ca level decreased (p < 0.05). The HCO_3- interacted with the Ca sources and thus, affected the Ca levels (p < 0.05). Bone ash, Ca, and P were downregulated in the low-Ca diet group compared with the case in the high-Ca diet group. Overall, the low dietary Ca supplementation led to greater growth performance. Furthermore, CaCl₂ appeared to be a better Ca source than CaCO₃ because of the greater digestibility of CP, Ca, and P, and improved EB.

• 화훼연구
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• 제16권3호
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• pp.222-227
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• 2008

석회질 및 규산질비료가 칼라의 생육, 절화품질, 줄기 경도 및 무름병 발생에 미치는 영향을 조사한 결과, 석회질비료 처리에서 잎의 생장 및 초장은 기비보다 엽면살포구에서 더욱 효과적이며, $CaCl_2$ 0.1% 처리구가 초장 111.7cm, 엽장 32.7cm, 절화장 118.6cm로 무처리 73.3cm, 23.8cm 및 79.6cm에 비해 효과적이었다. 석회질 비료가 줄기 경도에 미치는 영향은, 기비보다 엽면살포구에서 더욱 효과적이었으며, 엽면살포 처리중에서도 0.1% 처리구에서 가장 경도가 강했고 세포 및 조직이 규칙적이었다. 규산질비료처리구에서는 농용규산 $50kg{\cdot}10a^{-1}$ 처리구에서 잎의 생장, 생체중, 경도 및 절화품질 등 모든 변에서 양호하게 나타났다. 석회 및 규산질비료 시용과 무름병과의 관계는 엽면살포구보다 기비처리구에서 발병율이 낮았으나 두 처리구 모두 대조구보다는 다발하여 무름병과 상관관계는 없는 것으로 생각되었다. 칼라의 생육 및 개회에는 석회질비료가 규산질비료보다 효과적이었고 적정 농도는 0.l%였다.

• 농업과학연구
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• 제37권3호
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• pp.355-360
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• 2010

This experiment was focused to determine the effect of preharvest treatment of alkaine-reduced water (ARW) and $CaCl_2$ on the storage of oyster mushroom. ARW was prepared through electrolysis of water with $CaCl_2$ (0.1%) as salts and $CaCl_2$ (0.1%) was dissolved in deionized water. Mushrooms were harvested after two sprays of each solutions and quality factors including firmness and browning was determined during storage. The severity of browning was more greater when stored 20 days. Unlike $CaCl_2$, ARW containing 139 ug/L calcium was positive on maintaining freshness in terms of delay of firmness loss and respiration drop, keeping soluble carbohydrate content such as trehalose, and inhibiting the occurrence of browning. Browning, however, seemed to be more influenced by the developmental stage of mushroom at harvest. Even no significant difference was found, the level of ${\beta}$-glucan was low in ARW treatment compared to that of control. Further research including application and preparation methods of ARW is required to clarify the potential of ARW on keeping freshness of oyster mushroom through inhibiting tissue browning.

• 한국미생물·생명공학회지
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• 제13권4호
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• pp.391-395
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• 1985

내산성이 우수하고 citric acid와 SCP를 많이 생산할 수 있는 효모균주를 육종하기 위하여 구연산 생성효모인 Candide lipolytica의 종내 원형 질체융합 조건을 검토하였다. 배양기간과 효소처리조건을 최적화함에 의해 98%의 protoplast가 형성되었다. 3 % agar와 30mM $CaCl_2$를 함유한 재생용최소배지에 동일배지의 중층에 의해 약 20-30%의 protoplast가 재생되었다. 2개의 영양요구성이 상보적인 영양요구성원형질체, L-14($lys^-$)와 T-24($try^-$)에 100mM $CaCl_2$를 함유하는 30% PEG 6000을 $30^{\circ}C$ 에서 20분간 처리하여 4-5${\pm}$$10^{-4}$의 융합빈도가 얻어졌다.