• Title/Summary/Keyword: $Ca^{2+}$ movement

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Effect of sodium on transmembrane calcium movement in the cat ileal longitudinal muscle

  • Rho, Young-Jae;Yun, Il;Kang, Jung-Sook
    • Archives of Pharmacal Research
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    • v.10 no.2
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    • pp.80-87
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    • 1987
  • To get a better insight into the exxistence and the role of a Na-Ca exchange mechanism in smooth muscle, the effect of Na substitution with sucrose on tension development, cellular Ca uptake and $^{45}Ca$ efflux was investigated using isolated cat ileal longitudinal muscle strips. Experimental results were summarized as follows;1) Exposure of the cat ileal longitudinal muscle to Na-free solution induced a contraction, and the magnitude of the contraction increased after incubation of the muscle strips with ouabain ($2{\times10^{-}5}$M) for 1hr. 2) Cellular Ca uptake in Na-free solution increased with an increase in Na content of the Na-loading media, and a linear relationship existed between tissue Na content and cellular Ca uptake for 10 min 3) After tissues were equilibrated in PSS containing $^{45}Ca$ for 2hr, cellular Ca uptake decreased with rising the external Na concentration. 4)Removal of medium Na or inhibition of the Na-K pump decreased the rate of $^{45}Ca$ efflux. These results strongly suggested that Na substitution increases cellular Ca uptake and decreases the rate of $^{45}Ca$ efflux via a Na-Ca exchange mechanism.

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Calcium Movement in Carbachol-stimulated Cell-line (Calcium수송기전에 미치는 Carbachol의 영향)

  • Lee, Jong-Hwa
    • The Korean Journal of Pharmacology
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    • v.31 no.3
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    • pp.355-363
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    • 1995
  • It has been well known that the intracellular calcium concentration $([Ca^{2+}]_i)$ in living cell is very sensitive to live or to survive, but the transmembrane system of calcium ion, especially mechanism of calcium ion movement in unexcitable state has been little elucidated. Though many proposed theories for calcium ion transport have been reported, it is still unclear that how could the sustained maintenance in cytosolic calcium level be done in cell. Since one of possible mechanisms of calcium transport may be related to the acetylcholine receptor-linked calcium channel, author performed experiment to elucidate this mechanism of calcium influx related to cholinergic receptor in ml muscarinic receptor-transfected RBL-2H3 cell-line. 1) The effects of carbachol both on calcium ion influx and on the secretion of hexosaminidase were respectively observed in the manner of time-related or concentration-dependent pattern in this model. 2) The effects of several metal cations on calcium transport were shown in carbachol-induced cell-line. 3) Atropine was administered to examine the relationship between cholinergic receptor and calcium ion influx in this model. 4) PMA (Phorbol 12-myristate 13-acetate) or PTx (Pertussis toxin) was respectively administered to examine the secondary mediator which involved pathway of calcium ion movement in carbachol-induced cell-line. The results of this experiments were as follows; 1) Carbachol significantly stimulated both the calcium influx and the secretion of hexosaminidase in the manner of the concentration-dependent pattern. 2) Atropine potently blocked the effects of carbachol in concentration-response manner. 3) Administered metal cations inhibited the calcium influx in carbachol-stimulated this model to the concentration-related pattern. 4) PMA did not inhibit carbachol-induced secretion of hexosaminidase, but blocked the calcium influx in this cell-line. 5) The suppression of carbachol-induced hexosaminidase secretion was shown in PTx-treated cell -line.

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Float Zone Growth of Superconduction $Bi_2Sr_2CaCu_2O_8$ Fiber with Halogen Lamp (할로겐 램프 열원을 이용한 초전도성 $Bi_2Sr_2CaCu_2O_8$ Fiber의 Float Zone 성장)

  • 김철진;정준기
    • Korean Journal of Crystallography
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    • v.7 no.2
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    • pp.147-155
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    • 1996
  • Floating Zone Image furnace with halogen lamps as heat source has been made and applied to the growth of high-Tc superconductor Bi2Sr2CaCu2O8. The manufactured crystal growth equipment is composed of holder unit for setting the halogen lamps at the focal point of the elliptical mirror, image furnace with maximum temperature of 1800℃ with 1kW halogen lamps, cooling unit, feeding unit for supplying source material to the molten zone, pulling unit for crystal growth, and the control unit in the range of 2mm/hr -40 mm/hr vertical movement and 15rpm - 12rpm rotation. Bi2Sr2CaCu2O8 fibers have been grown with 300W halogen lamps and characterized with XRD, SEM and EDS. The growth condition was air atmosphere, growth speed 3∼4mm/hr, rotation speed of upper and lower part 20∼25 rpm. The fiber was composed of 20∼25 rpm matrix phase and secondary phases such as (Sr,Ca)CuO2 and (Sr,Ca)2CuO3.

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Preliminary three-dimensional analysis of tooth movement and arch dimension change of the maxillary dentition in Class II division 1 malocclusion treated with first premolar extraction: conventional anchorage vs. mini-implant anchorage

  • Park, Heon-Mook;Kim, Byoung-Ho;Yang, Il-Hyung;Baek, Seung-Hak
    • The korean journal of orthodontics
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    • v.42 no.6
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    • pp.280-290
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    • 2012
  • Objective: This study aimed to compare the effects of conventional and orthodontic mini-implant (OMI) anchorage on tooth movement and arch-dimension changes in the maxillary dentition in Class II division 1 (CII div.1) patients. Methods: CII div.1 patients treated with extraction of the maxillary first and mandibular second premolars and sliding mechanics were allotted to conventional anchorage group (CA, n = 12) or OMI anchorage group (OA, n = 12). Pre- and post-treatment three-dimensional virtual maxillary models were superimposed using the best-fit method. Linear, angular, and arch-dimension variables were measured with software program. Mann-Whitney U-test and Wilcoxon signed-rank test were performed for statistical analysis. Results: Compared to the CA group, the OMI group showed more backward movement of the maxillary central and lateral incisors and canine (MXCI, MXLI, MXC, respectively; 1.6 mm, p < 0.001; 0.9 mm, p < 0.05; 1.2 mm, p < 0.001); more intrusion of the MXCI and MXC (1.3 mm, 0.5 mm, all p < 0.01); less forward movement of the maxillary second premolar, first, and second molars (MXP2, MXM1, MXM2, respectively; all 1.0 mm, all p < 0.05); less contraction of the MXP2 and MXM1 (0.7 mm, p < 0.05; 0.9 mm, p < 0.001); less mesial-in rotation of the MXM1 and MXM2 ($2.6^{\circ}$, $2.5^{\circ}$, all p < 0.05); and less decrease of the inter-MXP2, MXM1, and MXM2 widths (1.8 mm, 1.5 mm, 2.0 mm, all p < 0.05). Conclusions: In treatment of CII div.1 malocclusion, OA provided better anchorage and less arch-dimension change in the maxillary posterior teeth than CA during en-masse retraction of the maxillary anterior teeth.

Salt Movement of Soils by Runoff in Green House Area (시설재배지 토양의 유거수에 의한 염류의 이동)

  • Kang, Bo-Koo;Kim, Hyun-Ju;Lee, Kyung-Ja;Kim, Jai-Joung;Hong, Soon-Dal
    • Korean Journal of Environmental Agriculture
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    • v.20 no.2
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    • pp.112-115
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    • 2001
  • Salt accumulation and movement by runoff ware studied in runoff resevior lysimeter constructed in a green house located in the area of Cheongju, Chungbuk province. Average runoff ratio of rainfall within period of this experiment was 58%. The average content of cations lost from field soil by runoff was in the order of $Ca^{2+}(27.12\;mg/L\;)$ > $K^+(9.18\;mg/L)$ > $Mg^{2+}(2.53\;mg/L)$ > $Na^+(1.89\;mg/L)$ and in the care anions $SO_4\;^{2-}(63.38\;mg\;/L)$ > $NO_3\;^-(25.40\;mg/L)$ > $Cl^-(4.19\;mg/L)$ > $PO_4\;^{3-}(3.18\;mg/L)$. Amounts of salt movement by runoff $SO_4\;^{2-}(140.2\;kg/ha)$, $Ca^{2+}(59.9\;kg/ha)$, $NO_3\;^-(56.1\;kg/ha)$, $K^+(20.3\;kg/ha)$, $Cl^-(9.3\;kg/ha)$, $PO_4\;^{3-}(7.0\;kg/ha)$, $Mg^{2+}(5.6\;kg/ha)$ and $Na^+(4.2\;kg/ha)$. The loss amount of $PO_4\;^{3-}$ was the lowest among the anions investigated in this experiment. $P_2O_5$ was accumulated on the soil surface due to strong affinity for the sorption site on the soil particle surface.

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Studies on the Effect of Cation on the Activity at the 5th Instar Larvae of Bombyx mori (5령유충의 배맥관운동에 대한 양이온의 영향에 관한 연구)

  • 윤종관;사기언
    • Journal of Sericultural and Entomological Science
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    • v.18 no.2
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    • pp.89-93
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    • 1976
  • The physiological saline solution for animals is known as Ringer's solution which is used for keeping the function of cold blooded vertebrate animals. Primaily the saline solution is used for the purpose of perfusion experiment in frogs. Later the saline solution is applied in several kinds of animals including human being with satisfactory results. However, this saline solution was introduced to silkworm and it was found that the result was not as successful as in the case of other animals and human being. Normally, in the case of silkworm, the physiological saline solution is prepared in order to maintain the normal function of separated organs and tissues. To this end, the saline solution is adjusted to contain the certain amount and strength of ions, osmosis pressure and hydrogen concentration. The most of cases, the physiological saline solution should be prepared so that the constituent of the solution be the same with the blood selium and body fluid. The hydrogen concentration in the ion element of the saline solution is adjustable by adding Na$\^$+/, K$\^$+/, Ca$\^$++/, Mg$\^$++/ which are followed by adding of buffer solution such as NaHCO$_3$and NaH$_2$PO$_4$. Determination of optimum concentration of cation in the physiological saline solution, and the optimum mixing rate of more than two kinds of cations are based on the movement of dorsal vessel in the silkworm larvae. The optimum concentration of cations in the solution is prepared by adding NaCl solution which is under zero point. However, this solution was further added with the different concentration of KCl and CaCl$_2$. By dropping the prepared solution on the 5th larvae, the effects of solution was measured. The measurement was done by observation of movement' of dorsal vessel and its time length, and the number of pulses. According to the experiment, it was found that when only NaCl solution was applied, the number of pulses is increased for a moment, and the pulse stopped after one hour or so. When KCl solution was added the time of pulse was prolonged and in the contrast, the number of pulses was slow down. If KCl and CaCl$_2$solutions are added the time of pulse was further prolonged. Even though the adding of KCl and CaCl$_2$are found to be effectible, the correlation between the concentration of solution and the movement of dorsal vessel was not observed. However, it was same in the case of adding Ca$\^$++/ or K$\^$+/. It was found that when Mg$\^$++/ was added to dorsal vessel the number of the pulses was not decreased although the prolonged time pulse was observed.

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Relationship of Cognitive Functions and Physical Activities in Persons with Chronic Stroke

  • Woo, Young-Keun;Hwang, Su-Jin
    • PNF and Movement
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    • v.10 no.4
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    • pp.1-7
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    • 2012
  • Purpose : The purpose of this study was to evaluate the relationship between physical performance, such as gait and postural control, and cognition on as assessed by clinical tools in individuals with chronic hemiparetic stroke. Methods : Twenty-six patients who had hemiparetic stroke participated in this study, and were evaluated four common clinical measurements, including the Berg balance scale (BBS), 10 meter walk test (10MWT), 6 minute walking test (6MWT), and Montreal cognitive assessment (MoCA). Multiple regression analysis was used BBS score, 10MWT, and 6MWT as the dependent variables; MoCA score, post-stroke duration, age, and affected side as independent variables. Results : In the regression equation of the BBS score, the correlation coefficient (r) was 0.875, the coefficient of determination (R2) was 0.786, and the MoCA score was the most important variable for determining the BBS score. In the regression equation for the 10MWT, ther was 0.888, the R2 was 0.999, and the MoCA score was the most important variable for determining 10MWT. Finally, the r was 0.777, the R2 was 0.998, and the MoCA score was the most important variable for determining 6MWT in the regression equation of the 6MWT. Conclusion : The results show that cognitive abilities affect gait proficiencies in individuals with chronic hemiparetic stroke. Therefore, these results suggest that cognitive tests are necessary for examining and evaluating the abilities of postural control and gait performance for chronic stroke patients in research and clinical environments.

Effect of Ammonium Nitrate Plus Potash in Comparison with Urea Plus Potash on the Yield and Content of Some Mineral Nutrient Elements of Chinese Cabbage (요소+칼리에 대비(對備)한 질산암모늄+칼리가 배추의 수량(數量) 및 무기성분함량(無機成分含量)에 미치는 영향(影響))

  • Oh, Wang-Keun;Kim, Sung-Bae
    • Korean Journal of Soil Science and Fertilizer
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    • v.18 no.4
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    • pp.407-412
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    • 1985
  • The effect of potash applied with different sources of nitrogen was experimented in pot soil culture on chinese cabbage (Brassica Pekinensis Rupr, Var. Fall 1984: Sammi Garak, Spring 1985: Jungang Summer lab.) in the fall, 1984 and in the spring, 1985. Results obtained are as follows; 1. Ammonium nitrate increased the yield of chinese cabbage more than urea did, and the effect of yield increase by ammonium nitrate was greater in the fall cultivation than in the spring. 2. The yield of chinese cabbage was positively correlated with the contents of K in the first (May 17, 1985) and second (June 9, 1985) thined cabbages (dry matter). It was also positively correlated with $NO_3-N$ content of the first thined cabbage and with K/Ca+Mg m.e, ratio of outer leaves of the harvested (June 27, 1985) cabbage, but negatively affected with Mg content of the outer leaves. 3. Correlations between K and $NO_3-N$ contained in the dry matter of first and second thined, and inner leaves of the harvested cabbage were learnt to be $r;0.9998^{**}$, r;0.4439, and $r;-0.7135^*$ respectively. The higher $NO_3-N$ content in the inner leaves of harvested cabbage was observed at K omitted ammonium nitrate plot where K was deficient, Ca and Mg contents were low. 4. The nutrient absorption and growth of chinese cabbage may take the following process. Nitrate nitrogen increases vegetative growth of the plant with enhanced K uptake and movement in to inner leaves and followed by replacement of Ca uptake and finally Mg uptake and its movement in to inner leaves.

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Study on the fabrication and the growth mechanism of Bi-2223 superconducting phase by diffusion method (확산법에 의한 Bi-2223 초전도상의 제조 및 성장기구에 관한 연구)

  • 최성환;최효상;한태희;황종선;한병성
    • Electrical & Electronic Materials
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    • v.7 no.4
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    • pp.281-288
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    • 1994
  • According to spread volume of B(BiPbCuO) layer, composition ratio and each stage of sintering process, we studied stability of high Tc superconductor phase and generation and growth movement of superconducting phase. The dual layer composed of SrCaCuO and BiPbCuO compound were prepared to develop the Bi-2223 superconductor[108K] through interaction and diffusion during sintering process. The dual layer samples were sintered at 830.deg. C for 0-210 hours. From the result, the optimum conditions were : spread volume(A:B=1:0.6), sintering time(210h) and composition ratio(A:S $r_{2}$C $a_{2}$C $u_{2}$- $O_{x}$, B:B $i_{1.9}$P $b_{0.5}$C $u_{3}$ $O_{y}$) at 830.deg. C.. C.C.C.

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Effects of Boganhwan Water Extract on the Cardiovascular System in Rabbits (보간환(補肝丸) 전탕액(煎湯液)이 가토(家兎)의 심혈관계(心血管系)에 미치는 영향(影響))

  • Ku, Won-Hae;Moon, Byung-Soon
    • The Journal of Internal Korean Medicine
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    • v.17 no.1
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    • pp.8-33
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    • 1996
  • The present experiment was designed to understand the effect of Boganhwan on the cardiovascular system in experimental animals. And thus the Blood pressure, isometric movement of atrium,Mg++-Ca++-ATPasc activity of sarcoplasmin reticulum. liver function, prothrombin time, and changes of blood composition were measured in the presence of Boganhwan. The results obtained here were as following: 1. The blood pressure decreased in the presence of SAMOOLTANG, and the decreasing effect was more stimulated by adding Bangpoong and Ganghwa(Boganhwan) 2. The oral administration of the drug for 7days also demonstrated the decreasing of blood pressure and the effect was stimulated by adding Bangpoong and Kanghwal. 3. The autonomic nerve blocking agents such as atropine and regitine did not demonstrate the effect on the drug action. 4. Boganhwan inhibited the cardiac isometric movement and rate by stimulating the Mg++-Ca++-ATPase activity of the heart sarcoplasmins reticulum. 5. Boganhwan increased the number of red blood cell, hematocrit percentage, hemoglobin concentration, and prothrombin time. 6. The drug stimulated the liver metabolism by stimulating the total ATPase activity. According to the results. Boganhwan demonstrated the decreased blood pressure and it also increased the hemoglobin concentration and the hematocrit percent. These effects stanches the hypertension. anemia, and cerebrovascular accident.

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