• Journal of Aquaculture
• /
• v.19 no.4
• /
• pp.254-260
• /
• 2006

A 6-week feeding trial was conducted to determine apparent protein and phosphorus digestibilities in order to evaluate nine different dietary protein sources in growing olive flounder, Paralichthys olivaceus. Nine diets containing fish meal analog ($BAIFA-M^{TM}$), white fish meal (WFM), flounder muscle powder (FMP), squid liver powder (SLP), leather meal (LM), soybean meal (SM), corn gluten meal (CGM), poultry by-product (PBP) or egg albumin(EA) were prepared by mixing a basal diet (BD) with one of nine test ingredients at the ratio of 7 to 3. Apparent protein digestibilities of FMP, SLP, WFM, SM, CGM, LM, PBP, $BAIFA-M^{TM}$, and EA were 94%, 92%, 86%, 82%, 75%, 72%, 72%, 71%, and 30%, respectively. Apparent phosphorus digestibilities of FMP, SLP, $BAIFA-M^{TM}$, LM, WFM, PBP, CGM, EA and SM were 77%, 72%, 65%, 55%, 54%, 50%, 20%, 20%, and 17%, respectively. Weight gain of fish fed FMP ($323^a$) was significantly higher than those of fish fed the other diets, and those of fish fed basal diet ($302^b$), SLP ($305^b$) and WFM ($308^b$) diet were significantly higher than those of fish fed SM ($274^c$), $BAIFA-M^{TM}\;(268^{cd}),\;PBP\;(261^{de}),\;LM\;(251^e),\;CGM\;(254^e)$ and EA ($181^f$). Based on the results of apparent protein digestibilities, apparent phosphorus digestibilities and weight gain, SLP, SM and $BAIFA-M^{TM}$ could be one of good protein source to replace fish meal in flounder diets.

• The Korean Journal of Mycology
• /
• v.27 no.6 s.93
• /
• pp.415-419
• /
• 1999

Cladobotryum varium infects the fruitbody of Flammulina velutipes during its cultivation. In the early stage of infection, white mycelia of C. varium partially attacked young fruitbody and eventually killed whole fruitbody during fruitbodies development. White fungi pathogen isolated from F. velutipes cultivation farm were investigated in pathogenicity and morphological feautres. The pathogen was identified as Cladobotryum varium. In asexual stage, $2{\sim}4$ conidiophores formed, $8.5{\sim}10.0{\times}16.1{\sim}17.0\;{\mu}m$ long and conidiospores with single septum were shaped in chains, $32.8{\sim}50.4\;{\mu}m$ in size. Size of chlamydospores were $12.7{\sim}18.0{\times}17.7{\times}48.1\;{\mu}m$ with $1{\sim}4$ septa.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
• /
• v.19 no.2
• /
• pp.67-73
• /
• 2013

This study investigated the quality changes of Samgyetang during storage, resulting from various degassing methods used at the packaging stage. Three different samples were prepared, one without any treatment (CON), a second with reduced head space volume by squeezing the pouch (RHS), and a third by flushing with nitrogen gas (NGF). These were retorted at $120^{\circ}C$ for 65 min under the F-value of approx. 8.0, and stored at $25^{\circ}C$ for 9 months. The oxygen ratio in the head space and the dissolved oxygen content of NGF samples tended to be lower than those of other samples over the storage period. Compared to the CON, the acid values of NGF and RHS samples were lower than the CON from month 3 and 6 (p<0.05). During storage, volatile basic nitrogen values of NGF and RHS samples increased slowly compared to the CON. To delay quality deterioration and extend the shelf-life of Samgyetang products, the reduction of oxygen content in the head space of the retort pouch (preferably by flushing with nitrogen gas) is recommended, even though no significant differences in sensory evaluation were observed among the samples during 9 months of storage (p>0.05).

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.23
• /
• pp.10513-10524
• /
• 2015

Background: Metastasis is a major reason for poor prognosis in patients with cancer, including hepatocellular carcinoma (HCC). A salient feature is the ability of cancer cells to colonize different organs. Long non-coding RNAs (lncRNAs) play important roles in numerous cellular processes, including metastasis. Materials and Methods: In this study, the lncRNA expression profiles of two HCC cell lines, one with high potential for metastasis to the lung (HCCLM3) and the other to lymph nodes (HCCLYM-H2) were assessed using the Arraystar Human LncRNA Array v2.0, which contains 33,045 lncRNAs and 30,215 mRNAs. Coding-non-coding gene co-expression (CNC) networks were constructed and gene set enrichment analysis (GSEA) was performed to identify lncRNAs with potential functions in organ-specific metastasis. Levels of two representative lncRNAs and one representative mRNA, RP5-1014O16.1, lincRNA-TSPAN8 and TSPAN8, were further detected in HCC cell lines with differing metastasis potential by qRT-PCR. Results: Using microarray data, we identified 1,482 lncRNAs and 1,629 mRNAs that were differentially expressed (${\geq}1.5$ fold-change) between the two HCC cell lines. The most upregulated lncRNAs in H2 were RP11-672F9.1, RP5-1014O16.1, and RP11-501G6.1, while the most downregulated ones were lincRNA-TSPAN8, lincRNA-CALCA, C14orf132, NCRNA00173, and CR613944. The most upregulated mRNAs in H2 were C15orf48, PSG2, and PSG8, while the most downregulated ones were CALCB, CD81, CD24, TSPAN8, and SOST. Among them, lincRNA-TSPAN8 and TSPAN8 were found highly expressed in high lung metastatic potential HCC cells, while lowly expressed in no or low lung metastatic potential HCC cells. RP5-1014O16.1 was highly expressed in high lymphatic metastatic potential HCC cell lines, while lowly expressed in no lymphatic metastatic potential HCC cell lines. Conclusions: We provide the first detailed description of lncRNA expression profiles related to organ-specific metastasis in HCC. We demonstrated that a large number of lncRNAs may play important roles in driving HCC cells to metastasize to different sites; these lncRNAs may provide novel molecular biomarkers and offer a new basis for combating metastasis in HCC cases.

• Journal of the Korea Concrete Institute
• /
• v.19 no.2
• /
• pp.189-197
• /
• 2007

Concrete tunnel lining must be designed to having the fireproof performance because the lining are sometimes exposed to very high temperature due to traffic accident. Such fire temperature may cause explosion of concrete, or collapse of tunnel structure. The purpose of this study is to obtain the fundamental fireproof behavior of fire resistance-engineered cementitious composites(FR-ECC) under fire temperature in order to use the fire protection material in tunnel lining system. The present study conducted the experiment to simulate fire temperature by employing 2 types of FR-ECC and investigated experimentally the explosion and cracks in heated surface of these FR-ECC. Employed temperature curve were hydro carbon(HC, ECl) criterion, which are severe in various criterion of fire temperature. The numerical analysis is carried out the nonlinear transient heat flow analysis and verified against the experimental data. The complex features of behavior in fire conditions, such as thermal expansion, plasticity, cracking or crushing, and material properties changing with temperature are considered. By the use of analytical model, the concrete tunnel subjected to fire loads were analyzed and discussed. With comparison of current concrete materials and FR-ECC, the experimental and analytical results of FR-ECC shows the better fire resistance performance than the other.

• ALGAE
• /
• v.21 no.1
• /
• pp.141-148
• /
• 2006

Benthic diatoms are known as a good food for shellfish in nature and in commercial hatchery of Jeju Island, Korea. Grammatophora marina is commonly found as dominant benthic micro-algae in Jeju coastal waters throughout the year. To know the best growth conditions of this species, culture was done in terms of three parameters; water temperature, salinity and nutrients. Each parameter was controlled by temperature of 15, 20 and 25°C; salinity of 25, 30 and 35 psu; and nutrient concentrations of 50, 100 and 200%. F/2 media was used with artificial seawater for the culture, which was continued for two weeks with L:D cycle 12:12 by using fluorescent light. Maximum specific growth rate was recorded 1.68 d–1 at temperature of 25°C with salinity of 35 psu and nutrient concentration of 200% on 6th day during the culture period. Maximum biomass was also observed 4.9 × 105 cells mL–1 in the same condition. This species may belong to the euryhaline and eutrophic habitat with warm condition. For nutritional aspects of this species, protein, lipid and carbohydrate were measured. The value of protein, lipid and carbohydrate was 4.96%, 15.82% and 5.65%, respectively. The antioxidant activities of 80% methanolic extract were 46.7%, 23.7% and 23.8% on DPPH (1,1-Diphenyl-2-picrylydrazy) radical, superoxide anion radical and hydrogen peroxide scavenging, respectively. Percentage metal chelating activity was 81.2%. Enzymatic extracts of Alcalase and Ultraflow showed remarkable scavenging activities on DPPH radical (86.5% and 57.2%, respectively), and superoxide anion scavenging activities were 45.3% and 41.4% from Kojizyme and Viscozyme extracts, respectively. Extract of Protomex revealed 24.8% activity on hydrogen peroxide and Neutase showed 30.8% on hydroxyl radical scavenging effects. Celluclast and Viscozyme extracts showed 33.2% and 32.1% activities on nitric oxide scavenging, respectively, while Alcalase showed 61.5% on metal chelating. This species contains higher lipids among the biochemical compounds and higher metal chelating activities from both 80% methanolic and enzymatic extracts.

• Journal of Mushroom
• /
• v.11 no.2
• /
• pp.53-62
• /
• 2013

A total of 35 phosphate solubilizing bacterial strains were isolated from waste mushroom bed of Agaricus bisporus in Buyeo-Gun, Chungnam and screened for the production of indole acetic acid (IAA). The best IAA producing strain was identified as Pantoea rodasii using 16S rRNA analysis. In addition to the IAA production, this strain could act as an efficient phosphate solubilizer (1100 ${\mu}g$ $ml^{-1}$ after 5 days of incubation) also. The selected strain was cultured under different conditions in order to assess the optimum conditions for maximum IAA production. The nutrient broth (NB) medium was recorded as the best medium, where the maximum IAA production (229 ${\mu}g$ $ml^{-1}$) was recorded at the start of stationary phase (12 hours after inoculation) of the bacteria growth. The performance of the strain was found to be maximum at the temperature of $30^{\circ}C$ followed by $25^{\circ}C$. IAA production was found to be increased with increasing tryptophan concentration (from 0.1 to 0.6%), however beyond this limit, a slight reduction in IAA production was observed. The strains' ability to produce IAA was further confirmed by extraction of crude IAA and subsequent TLC analysis. A specific spot from the extracted IAA preparation was found corresponding with the standard spot of IAA with same $R_f$ value. The results of HPLC analysis conducted in identifying and quantifying the IAA production more precisely, are in agreement with the results of the assessment done with colorimetric method. As revealed by the results of the pot experiment, the isolated strain could significantly enhance the growth (as measured by shoot and root growth) of mung bean plants compared to that of non-inoculated plants. Therefore it can be concluded that the present strain, Pantoea rodasii has great potential to be used as bio-inoculants.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.28 no.2
• /
• pp.202-210
• /
• 1983

These experiments were conducted to study the relationship among agronomic traits, varietal variation of grain-filling periods and the effect of environmental conditions on the grain-filling in the ten selected barley cultivars. The varietal variation of grain-filling periods was of significance: Tokak had the similar increment in both greenhouse and field plot, Suweon two row was believed to be an cultivar with short grainfilling period. Cultivars with higher grain-filling rates tended to. have significantly lower ash content. Ash content of a cultivar tended to be lower when grown under favorable condition. At maturity, the time of development of yellow or dark pigment at the crease base could be a possible criterion for physiological maturity determination. However, this requires further study. Average grain-filling period in the different conditions was constant, but varietal differences were 9 days in the greenhouse and 5 days in the field. The grain moisture content was not constant in accordance to both cultivars and growing conditions. Average accelerated days for heading and ripening periods were very constant in comparision with field and vinyl mulching suggests that the selected materials will be useful for genetic study on grain-filling periods. The cultivars and grown conditions with higher accumulated temperature per $100^{\circ}C$ from early grain-filling had larger grain-filling rates and heavier 1000 grain weight. This study showed that if the variation among and within cultivars is to be minimized, the greenhouse and vinyl mulching plots could be useful for genetic study on grain-filling periods in $BC_1$ and $F_2$ segregation.

• Food Science of Animal Resources
• /
• v.27 no.3
• /
• pp.284-289
• /
• 2007

This study was conducted to investigate the effects of copper and zinc supplementation on growth performance, nutrient digestibility, and meat and carcass characteristics in finishing pigs. A total of 72 $(Landrace{\times}Yorkshire{\times}Duroc)$ pigs (58.47 kg initial BW) were assigned to 6 treatments in a $2{\times}3$ factorial design $(Zn\;levels{\times}Cu\;levels)$. The sources of zinc and copper were Zn-methionine chelate and Cu-methionine chelate, respectively. Zinc levels used were 80 and 120 ppm and copper levels used were 10, 30 and 60 ppm. Throughout the entire experimental period, the average daily gain (ADG) and average daily feed intake (ADFI) were not significantly affected by Cu or Zn levels, or their relative levels. The G:F ratio was significantly affected by the relative levels of Cu and Zn (p<0.05), specifically at 30 ppm Cu and 120 ppm Zn. Dry matter digestibility was significantly affected by the levels of Cu (p<0.02), Zn (p<0.01) and the relative levels of each (p<0.04), in particular at 30 ppm Cu and 120 ppm Zn. Nitrogen digestibility was significantly affected by Zn levels (p<0.01) and the combination of 30 ppm Cu and 120 ppm Zn (p<0.03). The $L^*-value$, shear force, cooking loss and pH were not significantly affected by Cu levels, Zn levels or their combination. The $a^*- (p<0.04)\;and\;b^*- values (p<0.01)$ were significantly affected by Zn levels at 80 ppm. The Water hoding capacity was significantly affected by Cu and Zn in combination (p<0.01) at 10 ppm Cu and 120ppm Zn. The carcass weight, backfat thickness and carcass grade were not significantly affected by Cu levels, Zn levels or their relative levels. The carcass percentage was significantly affected by the combination (p<0.04) of 30ppm Cu and 120ppm Zn. In conclusion, dietary supplementation of Cu and Zn at 30 and 120 ppm, respectively, is effective for feed efficiency, nutrient digestibility and carcass percentage, while at the levels of Cu at 10 ppm and Zn at 120 ppm have effects on WHC.

• Journal of Oral Medicine and Pain
• /
• v.34 no.2
• /
• pp.217-225
• /
• 2009

As Pain is a comprehensive, biopsy chosocial phenomenon, improved understanding and successful management of pain need assessment of health-related quality of life and psychological states. The purpose of this study was to evaluate pain severity and pain-related interference to daily lives for patients with non-dental, orofacial pain(OFP) and a possible relation of OFP with psychological morbidity. Relation with such factors as gender, age, pain duration and diagnosis was also assessed. Inclusion criteria was all new patients with non-dental OFP attending the oral medicine.orofacial pain clinic of Dankook University Dental Hospital over 3 months' period, who completed the questionnaires of the Brief Pain Inventory (BPI) and Hospital Anxiety and Depression Scale (HADS). Prior to the first consultation, the patients were asked to fill out the questionnaire in the waiting room and were diagnosed through consultation and clinical examination. Total subjects were 163 with M:F ratio of 1:1.5 and mean age of 34.6${\pm}$17.7 years. Mean duration of pain was 13.3${\pm}$26.2 months and all patients were divided into; Trigeminal Neuralgia group (TN, N=8), Neuropathic Pain group (NeP, N=9), Persistent Idiopathic Facial Pain group (PIFP, N=8), and Temporomandibular Disorders group (TMD, N=138), subdivided into muscle problem (TMD-m, N=73), joint problem (TMD-j, N=24) and muscle-joint combined problem (TMD-c, N=41). OFP patients showed moderate pain severity and moderate pain-related interference. There was no gender difference in overall pain severity and interference and levels of anxiety and depression. Elderly patients aged ${\geq}$ 60 years showed higher pain severity (p<0.05). Patients with chronic pain ${\geq}$ 3 months reported more increased level of anxiety and depression than those with acute pain (p<0.05). Compared to TMD patients, patients with TN, NeP and PIFP suffered from higher level of pain and pain-related interference and reported higher level of anxiety and depression (p<0.05). Pain interference was closely correlated with their pain severity and with psychometric properties such as anxiety and depression. Pain severity was weakly correlated with levels of anxiety and depression. The results suggest a need for psychosocial assessment and support for successful management of OFP in addition to control of pain itself.