• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.213-213
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• 2017

High quality seed of proso millet that has high germination percentage, germination speed, and uniformity demanded to increases rates of mechanization in cereal crop cultivation. In order to improve germination characteristics, proso millet seeds were treated with red light, ultrasonication and liquid smoke (LS) solution that generated from hickory wood. All treatments were performed in seed priming solution with 100mM $NH_4NO_3$ at $15^{\circ}C$ for 24hrs under aeration condition. Seeds were exposed under light intensity of 2000 lux for 15m, 30m, 60m, and 120m in priming solution. Ultrasonic treatment was performed at 60%, 80%, and 100% intensity of 21.6 KHz for 5m, 10m, and 20m in priming solution. For LS treatment seed were soaked in 0%, 0.5%, 1.0%, 5.0% and 10.0% of diluted solution with $dH_2O$ or 100mM $NH_4NO_3$ solution. The effect of seed treatment was evaluated with germination percentage (GP), mean germination time (MGT), germination index (GI), germination rate (GR), Germination uniformity (GU) and heath seed percentage (HS). Our results demonstrate that red light (15min) or ultrasonication (21.6kHz, 5min) treatment improved MGT, GI, GR, and GU comparing to untreated control. Importantly, we show that LS treatments have significant effect on the health seedling and germination characteristics. Proso millet seeds that treated with 5% LS solution for 24hrs improves HS up to 97% that similar results obtained in 100mM $NH_4NO_3$ priming for 24hrs. The combined treatment with LS solution and 100mM $NH_4NO_3$ priming were not effective in all treatments. Our results demonstrate that treating seeds with LS or 100mM $NH_4NO_3$ priming or ultrasonication improves germination characteristics. The methods described here will help advance research using this species by increasing the germination performance at which successive seed pellet process.

• Magazine of the Korea Concrete Institute
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• v.7 no.3
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• pp.139-148
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• 1995

The test results from three one fourth scale models using high strength Reinforced Concrete $f_x=704\;kg/cm^2,\;f_y=5.830\;kg/cm^2$ are presented. Such specimens are considered to represent the critical 3 storics of 60-story tall building of a structural wall system in area of high seismicity respectively. They are tested under inplane vertical and horizontal loading. The main varlable is the level of axial stress. The amounts of vertical and horizontal reinforcement are identical for the three walls testcd. The cross-section of all walls is barbell shape. The aspectratio($h_w/I_w$) of test specimen is 1.8. The aim of the study is to investigate the effects of levels of applied axial stresses on the inelastic behavior of high-strength R /C tall walls. Experimental results of high strength R /C tall walls subjected to axial load and simulated sels rnic loading show that it is possible to insure a ductlle dominant performance by promotmg flex ural yielding of vertical reinforcement and that axial stresses within $O.21f_x$ causes an increase in horizontal load-carrying capacity, initial secant st~ffness characteristics, but an decrease in displacement ductility. energy dissipation index and work damage index of high strength K /C tall walls

• Korean Journal of Animal Reproduction
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• v.20 no.2
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• pp.207-214
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• 1996

To examine the developmental capacity of manipulated embryos after ultrarapid refreezing and thawing, mouse embryos were biopsied at 4-cell stage, frozen twice at 4-cell and morula stages, respectively, and then transferred to rec-ipients. Single blastomeres were biopsied from 4-cell embryos by a modified aspiration method. Biopsied 4-cell embryos were equilibrated into freezing medium at room temperature for 2.5 min, loaded into 40 $\mu$I of freezing medium in 0.25 ml plastic straw and then directly immersed into liqiud nitrogen. Freezing medium for 4-cell embryos consisted of 4.0 M ethylene glycol and O.25 M sucrose in dPBS supplemented with 6 mg/lm BSA. Morulae were frozen into freezing medium containing 5.0 M glycerol instead of ethylene glycol. Thawing was conducted by agitating each straw in 3TC water for 20 sec. The c content of each straw was expelled into 0.5 ml of dilution medium, which consisted of 0.25 M sucrose and 3 mg/ml BSA in dPBS. The thawed embryos were rehydrated in dilution medium for 10 min, washed 3 times with dPBS and then cultured in M16 medium at 37$^{\circ}C$, 5% CO$_2$ in air. Blastocysts that developed from frozen or refrozne biopsied embryos were transferred to recipients on Day 3 of pseudopregnancy, respectively. In vitro and in vivo developmental rates of the biopsied and intact 4~cell embryos after freezing and thawing were 78 (10l/130) and 25% (10/40), and 91 (114/125) and 30% (12/40), respectively. Although the rates of in vitro development of biopsied and intact embryos to blastocyst stage were significantly different after freezing and thawing (P

• Food Science and Preservation
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• v.9 no.1
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• pp.102-108
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• 2002

The purpose of this study was to investigate antibacterial activity of extracts of Cordyceps spp.(Paecilomyces japonica and Cordyceps militaris), mugwort and pine needle. Fruiting body and mycelium of Cordyceps spp., mugwort and pine needle were extracted with water and 70% ethanol. Antibacterial activities of each extracts against 3 kinds of Gram positive (Bacillus subtilis, Listeria monocytogenes and Staphylococcus aureus) and 3 kinds of Gram negative pathogenic bacteria(Escherichia coli O157 : H7, Shigella sonnei and Salmonella typhimurium) were tested. The yields of water and ethanol extracts of fruiting body (39∼58%) were 2.4 ∼4.4 times higher than mycellium(9∼24%) in Cordyceps sup., while those of mugwort and pine needle were less than 9%. Ethanol extract of P. japonica mycelium(JFE) had antibacterial to S. monocytogenes at 1% level and ethanol extract of C. militaris fruiting body (MFE) had antibacterial to S. aureus at 3% level. Ethanol extract of mugwort was antibacterial against L monocytogenes and S. aureus at 1% level. Water extracts of Cordyceps spp.(P. japonica and C. militaris) and mugwort had no antibacterial activity against tested bacterial strains. Water extract of pine needle had antibacterial activity against all bacterial strains except E. coli and ethanol extract had antibacterial activity against all tested bacterial strains at 1% level. Pine needle extracts had the most wide antibacterial spectrum against bacterial strains used for this experiment. Growth inhibiting activities of pine needle extracts were higher in ethanol extract than water extract for most of tested bacteria in tryptic soy broth.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.9
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• pp.1271-1276
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• 2004

For Exp. 1, one hundred twenty Duroc${\times}$ Yorkshire${\times}$Landrace pigs (6.34${\pm}$ 0.70 kg average initial BW) were used in a 35 d growth assay. There were six pigs per pen and four pens per treatment. Dietary treatments included 1) NC (negative control; basal diet), 2) PC (positive control; added 5% HP $300^{(R)}$, HAMLET PROTEIN A/S, as protein source), 3) PSP 2.5 (added 2.5% $Pepsoygen^{(R)}$, Genebiotech Co. Ltd., as protein source), 4) PSP 5.0 (added 5% $Pepsoygen^{(R)}$ as protein source), and 5) PSP 7.5 (added 7.5% $Pepsoygen^{(R)}$ as protein source). For d 21, ADFI was increased in pigs fed PSP diets compared with PC diet (p<0.05). ADG was tended to improve as concentration of PSP in the diets was increased. For d 21-35 and entire experimental period, pigs fed PSP diets had more feed intake than pigs fed NC or PC diets (p<0.05). For the whole period, ADG was improved in PSP treatments compared with NC treatment (Linear effect, p=0.05). On d 21 and d 35, digestibilities of DM and N were higher (p<0.05) for pigs fed PSP diets than pigs fed NC diet and PSP 2.5 treatment was the highest among the treatments. On d 21, digestibility of N was significantly improved in PSP treatments compared with PC treatment (p<0.05). For Exp. 2, five Duroc${\times}$Yorkshire${\times}$Landrace barrows (8.74${\pm}$0.22 kg average initial BW) were surgically fitted with a simple T-cannulas approximately 15 cm prior to the ileo-cecal junction. The experimental designs were 5${\times}$5 latin squares with pigs and periods as blocking criteria. Dietary treatments were same as Exp.1. Generally, apparent ileal digestibilities of DM and N were higher for pigs fed PSP diets than pigs fed NC or PC diets (p<0.05) and PSP 5.0 and 2.5 treatments were the highest among the treatments, respectively. Apparent ileal digestibilities of the whole amino acids were increased in PSP treatments compared with NC treatment (Quadratic effect, p${\leq}$0.05). Also, histidine, lysine and threonine digestibilities in essential amino acids and alanine, asparatic acid and glycine digestibilities in non essential amino acids were significantly higher for pigs fed PSP diets than pigs fed PC diets (p${\leq}$0.05). In conclusion, feeding processed soy protein to weanling pigs increased growth performance, nutrient digestibility and apparent ileal digestibilities of DM, N and most amino acids.

• The Korean Journal of Pharmacology
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• v.31 no.2
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• pp.195-206
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• 1995

The study was undertaken to examine the possibility of the involvement of $K^+$ channels in the mechanism of relaxant-action of imipramine on the isolated canine detrusor muscle strips. Canine urinary bladder were isolated, and smooth muscle strips of 15 mm long and 2 mm wide from the mid-portion of anterior wall were made in the Tyrode solution of $0{\sim}4^{\circ}C$. The strips were prepared for isometric myography in Biancani's isolated muscle chamber containing 1 ml of Tyrode solution, which was maintained with pH 7.4 by aeration with $95%\;O_2/5%CO_2\;at\;37^{\circ}C$. RP 52891, a non-specific $K^+$ channel opener, concentration-dependently suppressed the spontaneous phasic contractions of the detrusor strips. Imipramine, a tricyclic antidepressant, also reduced the spontaneous contractions in a concentration-dependent manner. RP 52891 was more potent than imipramine(p<0.05), and Imipramine was more efficient than RP 52891(p<0.05).Procaine, a voltage-dependent $K^+$ channel blocker, glibenclamide, an ATP-dependent $K^+$ channel blocker, and apamin, a calcium-dependent $K^+$ channel blocker antagonized the relaxant effect of RP 52891, but not of imipramine. Imipramine reduced the electric field stimulation (EFS) -induced contractions concentration-dependently. None of the $K^+$ channel blockers employed for this study, procaine, glibenclamide or apamin antagonized the inhibitory action of imipramine on the EFS-induced contraction. These results suggest that in canine detrusor, the $K^+$ channels of the characteristics of voltage-dependent, ATP-dependent and/or calcium-dependent are exist, and the inhibitory action of imipramine on the contractility of the detrusor is independent from the $K^+$ channels.

• Food Science and Biotechnology
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• v.14 no.4
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• pp.474-478
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• 2005

Because it possesses anti-inflammatory, antifungal, antiviral, and tissue regenerative properties, propolis has been used for thousands of years in folk medicine for multiple purposes. Although the antimicrobial activity of propolis has already been demonstrated, very few studies have been conducted on bacteria of clinical relevance in dentistry. The aim of this study is to evaluate the antimicrobial, anti-inflammatory, and anti-oxidative activities of 0.1% and 1.0% propolis, both of water-extracted (proAQ) and ethanol-extracted (proAL) propolis, for industrial applications. In studies of antimicrobial activity, the growth of Staphylococcus aureus ATCC 35556, Salmonella enteritidis ATCC 12021, Escherichia coli O157:H7, and Candida parapsilosis KCCM 35428, all general food or clinical pathogens, were tested. The culture medium used was trypticase soy broth including 0.6% yeast extract; after 6 hr of incubation, the turbidities were measured at 620 nm with a spectrophotometer. The results indicate that the antimicrobial effects of both 1.0% proAQ and 1.0% proAL were greater against the growth of S. aureus ATCC 35556 and C. parapsilosis KCCM 35428 rather than those of S. enteritidis ATCC 12021 and E. coli O157:H7. Additionally, it appears that the anti-inflammatory effects of proAL are greater than those of proAQ. The anti-inflammatory effects were evaluated by measurement of the inhibition of hyaluronidase activity in vitro. At a 1% concentration, the anti-inflammatory effects of proAL were greater than those of proAQ. Finally, the anti-oxidative effects of 1% and 10% solutions of each extract sample were measured according to the TBA method at $40^{\circ}C$ for 1, 2, 3, and 5 days and were compared with 1.0% BHT. The results indicate that the anti-oxidative effects at 0.1% for both proAQ and proAL were not significantly different than the anti-oxidative effects at 1.0% BHT (p<0.05). Thus, it appeared that the alcohol-extracted propolis had greater antimicrobial, anti-inflammatory, and anti-oxidative effects than the water-extracted propolis. This is based on the presumption that major biofunctional components were fat-soluble, rather than water-soluble.

• Applied Chemistry for Engineering
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• v.30 no.4
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• pp.435-444
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• 2019

In this study, two types of nonionic saccharide biosurfactants, GP-6 and GP-7, were prepared from coconut oil and the structure of resulting products was investigated by FT-IR, $^1H-NMR$ and $^{13}C-NMR$ spectrophotometer. The interfacial properties of GP-6 and GP-7 were found to be excellent from interfacial property measurements such as critical micelle concentration, static and dynamic surface tensions, interfacial tension, emulsification power, wetting property and foam stability. Detergency test evaluated by using a Terg-o-tometer showed moderately good detergency compared to that of conventional surfactants used in detergent formulations. Biodegradability, acute oral toxicity, acute dermal irritation and acute eye irritation tests revealed that both surfactants possess excellent mildness and superior environmental compatibility indicating the potential applicability to detergent products formulations. In particular, GP-6 can be considered as a strong candidate in detergent formulations since it is more surface active, mild and readily biodegradable than GP-7.

• Korean Journal of Poultry Science
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• v.39 no.3
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• pp.183-193
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• 2012

This study was conducted to investigate the effect of feeding the electrolytic materials on blood and carcass traits of broiler during transportation exposed under intense heat condition in summer. The broilers were selected on the day when the outside temperature was about $32^{\circ}C$ to provide heat stressed environment. Broilers reared for 33 d were selected and fed with the electrolytic materials ($NaHCO_3$, NaCl, KCl) for 2 days. Treatments were as follows; feeding the underground water for control, $NaHCO_3$ (1.0%) + NaCl (0.5%) for treament 1, KCl (0.5%) + NaCl (0.5%) for treatment 2, KCl (1.0%) + NaCl (0.5%) treatment 3, KCl (0.5%) + $NaHCO_3$ (1.0%) + NaCl (0.5%) for treatment 4 and KCl (1.0%) + $NaHCO_3$ (1.0%) + NaCl (0.5%) for treament 5. pH of chicken meat increased for treatments group of electrolytic material, especially, that of treatment 3 was highest when compared to the other treatments. The frequency rate (%) of $1^+$ quality grade were 33.3, 60.0 and 83.3% at control, treatment 3, 4 and treatment 5, respectively. Occurrence rates of PSE were 50% for control and 13.3% for treatment 5. Corticosterone increased at the post-harvest period compared to the pre-harvest period of broiler and have small disparity between pre-and post-harvest only except treatment 3 when compared to control. $pCO_2$ partial pressure of blood at the pre-harvest period was low in all treatments by heat stress, the disparity value of control was high for control, and those of treatment 4 and 5 were low compared to other treatments.

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.3
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• pp.179-186
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• 2014

The objective of this study was to determine the best performing switchgrass (Panicumvirgatum L.) cultivar with three different seeding dates as a bioenergy source in Republic of Korea. Split-plot in time with three replications was performed and three switchgrass cultivars, Carthage (CT), Cave-in-Rock (CIR), and Forestburg (FB) were used in this experiment from 2009 to 2012. Plots were seeded on April 23, May 4, and May 13, 2009 and were harvested once in November each year. No fertilizer was applied to the field for the first year; however, in second and third years (June 2010 and May 2011, respectively), N, $P_2O_5$ and K2O fertilizers were applied in 67,45 and 90 kg $ha^{-1}$, respectively. Soil pH (5.9) and climate condition including temperature ($10.4{\sim}17.5^{\circ}C$) and precipitation (89.4~109.8 mm $month^{-1}$) were suitable for switchgrass cultivation. Total dry matter yields were higher in CT and CIR compared to FB and were 16.9, 15.9, and 4.5 ton $ha^{-1}$, for CT, CIR, and FB, respectively (p<0.0001). The samples were analyzed for dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), crude fiber (CF), ether extract (EE), and ash. No significant differences in energy content (p = 0.96) and chemical composition among cultivars (p>0.05) were found. Seeding dates did not affect DM yield (ton $ha^{-1}$), chemical composition and energy content significantly (p>0.05). Significant difference was observed for heights among CT, CIR, and FB (177.59, 169.98, and 94.89 cm, respectively, p = 0.0002). In conclusion, based on soil characteristics and climate condition in Korea compared to other countries, switchgrass can be cultivated successfully. Considering dry matter yield and energy content of these three cultivars of switchgrass CT and CIR adapted better to climate in Middle Eastern of Republic of Korea than Forestburg for bioenergy purpose.