• Journal of Microbiology and Biotechnology
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• 제24권4호
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• pp.545-555
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• 2014

It has been previously demonstrated that laccases exhibit great potential for use in several industrial and environmental applications. In this paper, two laccase isoenzyme genes, lccB and lccC, were cloned and expressed in Pichia pastoris GS115. The sequence analysis indicated that the lccB and lccC genes consisted of 1,563 and 1,584 bp, and their open reading frames encoded 520 and 527 amino acids, respectively. They had 72.7% degree of identity in nucleotides and 86.7% in amino acids. The expression levels of LccB and LccC were up to 32,479 and 34,231 U/l, respectively. The recombinant laccases were purified by ultrafiltration and $(NH_4)_2SO_4$ precipitation, showing a single band on SDS-PAGE, which had a molecular mass of 58 kDa. The optimal pH and temperature for LccB were 2.0 and $55^{\circ}C$ with 2,2'-azinobis-[ 3-ethylbenzthiazolinesulfonic acid (ABTS) as a substrate, whereas LccC exhibited optimal pH and temperature at 3.0 and $60^{\circ}C$. The apparent kinetic parameters of LccB were 0.43 mM for ABTS with a $V_{max}$ value of 51.28 U/mg, and the Km and $V_{max}$ values for LccC were 0.29 mM and 62.89 U/mg. The recombinant laccases were able to decolorize five types of dyes. Acid Violet 43 (100 g/ml) was completely decolorized by LccB or LccC (2 U/ml), and the decolorization of Reactive Blue KN-R (100 g/ml) was 91.6% by LccC (2 U/ml). Thus, the study characterizes useful laccase isoenzymes from T. versicolor that have the capability of being incorporated into the treatment of similar azo and anthraquinone dyes from dyeing industries.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.394-397
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• 2000

Styrene을 제거하기 위한 biofilter가 연구되었다. 운전 초기에 활성슬러지를 접종함으로써 start-up 기간을 24시간으로 줄일 수 있었다. 운전중 질소원 고갈 현상이 발생하였고 첨가된 ammonium sulfate양에 따라 제거된 styrene의 양을 정량적으로 구하여 이것을 biofilter의 장기운전에 이용할 수 있었다. Styrene의 maximum elimination $capacity(EC_{max})$와 critical elimination $capacity(EC_{cr})$는 각각 4.8kg $C\;/m^3{cdot}day$, 1.248kg $C\;/m^3{cdot}day$이었으며 styrene 농도 400ppmv까지를 분해하는데 EBRT 1min으로 제거율 95% 이상을 달성할 수 있었다.

• 미생물학회지
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• 제24권3호
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• pp.302-307
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• 1986

내성 P.aeruginosa에 의해 생성되는 항생물질 파괴효소인 cephalosporinase는 cephaloridine을 기질로 하여 효소활성도는 $45^{\circ}C$와 pH 8.5에서 각기 최고의 활성도를 나타내었다. SDS. carbenicillin 빛 cephalosporin계 항생체에 의해 효소의 활성도는 현저하게 억제되었으며 효소의 cephaloridine에 대한 $V_{max}$ 값을 100으로 하였올때 cefo p perazone의 $V_{max}$ 값은 2.8로 나타났다. 측정된 효소의 분자량은 $37.500{\pm}3,000$이었다. 다른 Gram음성셰균이 분비하는 cephalosporinase와 비교해본 결과 균체 자체의 특성에 의해 cepha]osporinase는 서로 다름을 알 수 있였 으며 이로 인하여 각각의 균체가 cephalosporin계 항생제에 대한 내성의 차이를 나타내는 하나의 중요한 원인으로 판단되었다.

• 한국통신학회논문지
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• 제31권3C
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• pp.219-227
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• 2006

본 논문에서는 이동통신사업자가 서비스 종류에 따라 서로 다른 트래픽 요금기준을 적용하는 점을 이용하여 시스템에서의 전체적인 요금을 최대화하는 패킷 스케줄링 알고리즘을 제안한다. 제안하는 알고리즘은 스케줄링되는 각 사용자의 채널상태를 나타내는 SIR(signal to interference ratio)값과 각 사용자가 요청하는 서비스 종류에 근거하여 각 사용자의 요금 함수치를 산출하여 스케줄링한다. 제안하는 알고리즘의 성능을 측정하기 위하여 HSDPA 시스템에서 Max C/I, 비례공정 알고리즘, 라운드 로빈 알고리즘과 비교 분석한다. 성능 비교분석 결과 제안하는 알고리즘이 우수한 수율(throughput)을 유지하면서 이동통신 사업자의 수익(revenue)도 최대화됨을 보여준다.

• 한국통신학회논문지
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• 제32권11C호
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• pp.1111-1118
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• 2007

본 논문은 다중 사용자 시스템에서 좋은 채널을 가진 사용자를 선택함으로써 얻을 수 있는 다이버시티, 즉 다중 사용자 다이버시티로부터 증대되는 처리율 (throughput)에 대해 새로운 접근 방식을 이용하여 분석하였다. 이와 같은 다중 사용자 다이버시티 이득으로부터 도출되는 처리율의 증가를 근사적 형태의 식으로 유도하기 위하여, 본 논문에서는 Puiseux 급수를 이용하였고 이를 통해 단순화된 맺음식 (closed form)의 결과를 얻을 수 있었다. 이 결과식은 다중 사용자 다이버시티 이득이 신호 대 잡음비 (SNR)에 독립적이라는 것을 보여주고 있다. 또한 본 논문에서는 위의 결과를 Max C/I과 proportional fair scheduling과 같은 스케줄링 알고리즘에 확대 적용하였다. 그리고 제시한 결과식을 모의실험과 비교하여 검증하였다.

• 약학회지
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• 제48권6호
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• pp.379-383
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• 2004

Biphenyl dimethyl dicarboxylate (DDB) has been used for the treatment of chronic viral hepatitis B and drug-induced hepatitis through the inhibition of lipid peroxidation and c ovalent binding of drug metabolites to lipids of microsomes. The bioequivalence of two DDB products was evaluated according to the guidelines of KFDA. The test product was Hepaphil soft capsule(R) made by KMS Pharm. Co. Containing 3 mg DDB and the reference product was Nissel tablet(R) made by Taerim Pharm. Co. Containing 25 mg DDB. Twenty healthy male subjects, 25.4(22~30) years old and 66.7(54~77)kg, were divided into two groups and a randomized $2{\times}2$ cross-over study was employed. After two tablets or two capsules were orally administered, blood was taken at predetermined time intervals and the concentration of DDB in plasma was determined using a validated HPLC method with UV detector. Two pharmacokinetic parameters, $AUC_t$ and $C_{max}$, were calculated and analyzed statistically for the evaluation of bioequivalence of the two products. Analysis of variance was carried out using logarithmically transformed parameter values. The 90% confidence intervals of $AUC_t$ and $C_{max}$ were log 0.91~log1.00 and log 1.05~log 1.15, respectively. These values were within the acceptable bioequivalence intervals of log 0.8 to log 1.25. Thus, the criteria of the KFDA guidelines for the bioequivalence was satisfied, indicating that Hepaphil soft capsule is bioequivalent to Nissel tablet.

• Journal of the korean society of oceanography
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• 제33권4호
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• pp.196-204
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• 1998

$^{14}$C uptake experiments were carried out in and around the cold water mass in the southern part of the Korean East Sea in August and October 1995 to assess spatial and seasonal variability of primary productivity and its relation to physical and chemical factors. The cold and high saline water mass in the bottom layer extended upward to the surface layer and developed along the eastern coast of Korea in August. Chlorophyll-a concentration was maintained high in the cold water mass through August to October and its maximum concentration was 6.3 ${\mu}$g 1$^{-1}$ at Stn. 209-4 in August. Primary productivity and daily primary productivity ranged from 0.29 to 8.02 mgC m$^{-3}$ hr$^{-1}$ and from 58.3 to 63.1 mgC m$^{-2}$ d$^{-1}$, respectively, throughout the study period. Primary productivity of the cold water mass was higher than that of offshore waters in both summer and autumn seasons. P$_{max}$ and I$_{max}$ of the cold water mass in August were higher than those in October, except Stn. 208-5. These results suggest that high primary productivity in the cold water mass may be established by the upwelled nutrients and light adaptaion to convected phytoplankton due to upwelling of the bottom waters.

• Progress in Superconductivity
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• 제13권3호
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• pp.169-177
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• 2012

Distribution of the local properties in GdBCO and YBCO coated conductors was investigated using Low-temperature Scanning Laser and Hall Probe Microscopy (LTSLHPM). We prepared GdBCO and YBCO coated conductors to study the spatial distribution of the current density in a single bridge. Inhomogeneity of the ${T_c}^{max}$ in the bridge was analyzed from experimental results of Scanning Laser Microscopy (SLM) near the superconducting transition. The local transport and screening current in the bridge were also investigated using Scanning Hall Probe Microscopy (SHPM). A series of line scans of SLM of the GdBCO and YBCO sample showed that lines with more inhomogeneous distributions of ${\delta}V$ had more inhomogeneous distributions of ${T_c}^{max}$. The defect of the superconducting layer of the GdBCO sample caused by damage to the substrate affected the current flow. And we could analyze the redistribution of the current density using SLM and SHPM.

• 대한기계학회논문집B
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• 제22권3호
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• pp.267-279
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• 1998

A numerical study was made of flow behind a circular cylinder in a uniform flow, where the cylinder was rotationally oscillated in time. The temporal behavior of vortex formation was scrutinized over broad ranges of the two externally specified parameters, i.e., the dimensionless rotary oscillating frequency (.110.leq. $S_{f}$.leq..220) and the maximum angular amplitude of rotation (.theta.$_{max}$=15 deg., 30 deg. and 60 deg.). The Reynolds number (Re= $U_{{\inf}D}$.nu.) was fixed at Re=110. A fractional-step method was utilized to solve the Navier-Stokes equations with a generalized coordinate system. The main emphasis was placed on the initial vortex formations by varying $S_{f}$ and .theta.$_{max}$. Instantaneous streamlines and pressure distributions were displayed to show the vortex formation patterns. The vortex formation modes and relevant phase changes were characterized by measuring the lift coefficient ( $C_{L}$) and the time of negative maximum $C_{L}$( $t_{-C}$$_{Lmax}$) with variable forcing conditions.s.tions.s.s.s.

• Biomolecules & Therapeutics
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• 제9권4호
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• pp.298-306
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• 2001

To develop a novel transdermal delivery system of loxoprofen (LP), a potent antiinflammatory and analgesic agent, the effects of vehicle composition and drug loading dose on the skin permeation property were investigated. And in vivo skin absorption property studied by analysing the $C_{max}$ and AUC was investigated after applying the developed plaster systems on rabbit back skin. Addition of isopropyl myristate (IPM) and IPM-diethylene glycol monoethyl ether (DGME) cosolvent in the plaster showed higher permeation rates than those from propylene glycol laurate-DGME cosolvent systems. As the concentration of LP in the plaster increased from 0.56 mg/$\textrm{cm}^2$ to 1.19 mg/$\textrm{cm}^2$, the drug release and skin permeation rates increased linearly. At loading dose of 1.19 mg/$\textrm{cm}^2$, the flux reached 35.6 $\mu$g/$\textrm{cm}^2$/hr. New LP plasters showed a good adhesive property onto skin, and showed no crystal formation. The AU $C_{0-24hr}$ and $C_{max}$ after dermal application of LP plaster (60 mg/70 $\textrm{cm}^2$) were found to be 6951$\pm$230 ng.hr/ml and 400$\pm$44 ng/ml, respectively. And the plasma concentration maintained above 300 ng/ml up to 24 hr period. In the carrageenan-induced rat paw edema test, LP plaster showed similar inhibition rate with marketed ketoprofen (Ketoto $p^{R}$) plaster.aster.r.