• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.578-581
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• 2001

A treatability study was conducted using a hydrocarbon-contaminated soil for the oPtimization of bioremediation strategy best fit to a given set of contamination. The applicability of nutrients, biosurfactant, and oil-degrading microorganisms were examined by monitoring $CO_2$ evolution and oil degradation The addition of inorganic nutrients in the form of slow released fertilizer accelerated the initial rate of $CO_2$ evolution by a factor of 3. The application of oil-degrading microorganisms did not significantly increased $CO_2$ evolution or biodegradation efficiency. Application of a commercial biosurfactant was most effect in terms of the total $CO_2$ evolution and the oil degradation rate. The results indicate that $CO_2$ evolution measurement was found to be a simple and reliable countermeasure of crude oil hydrocarbon mineralization for the rapid determination of the best-fit bioremediation strategy.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1996년도 International Conference on Agricultural Machinery Engineering Proceedings
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• pp.947-952
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• 1996

The effect of fluctuating temperature on the respiration of vegetables has been investigated. Spinach was selected as the experimental material because of its high respiratory activity and kept under the condition that temperature changed alternately at low and high levels every 4 hours. The low-high level temperature combination was set in $1-10^{\circ}C,{\;}1-20^{\circ}C{\;}and{\;}1-30^{\circ}C$. Respiration was evaluated in terms of quantity of $CO_2$ evolved from spinach. The evolution rate of $CO_2$ was determined by a change in its concentration. The evaluation rate of $CO_2$ followed closely the temperature change. In the temperature combinations at $1-10^{\circ}C{\;}and{\;}1-20^{\circ}C$, the relationship between $CO_2$ evolution rate and temperature was found to be able to express by Arrhenius law, while at $1-30^{\circ}C$, it did not obey the law.

• Journal of Microbiology and Biotechnology
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• 제21권5호
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• pp.519-524
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• 2011

The correlation between alcoholic fermentation rate, measured as carbon dioxide ($CO_2$) evolution, and the rate of hydrogen sulfide ($H_2S$) formation during wine production was investigated. Both rates and the resulting concentration peaks in fermentor headspace $H_2S$ were directly impacted by yeast assimilable nitrogenous compounds in the grape juice. A series of model fermentations was conducted in temperature-controlled and stirred fermentors using a complex model juice with defined concentrations of ammonium ions and/or amino acids. The fermentation rate was measured indirectly by noting the weight loss of the fermentor; $H_2S$ was quantitatively trapped in realtime using a pre-calibrated $H_2S$ detection tube which was inserted into a fermentor gas relief port. Evolution rates for $CO_2$ and $H_2S$ as well as the relative ratios between them were calculated. These fermentations confirmed that total sulfide formation was strongly yeast strain-dependent, and high concentrations of yeast assimilable nitrogen did not necessarily protect against elevated $H_2S$ formation. High initial concentrations of ammonium ions via addition of diammonium phosphate (DAP) caused a higher evolution of $H_2S$ when compared with a non-supplemented but nondeficient juice. It was observed that the excess availability of a certain yeast assimilable amino acid, arginine, could result in a more sustained $CO_2$ production rate throughout the wine fermentation. The contribution of yeast assimilable amino acids from conventional commercial yeast foods to lowering of the $H_2S$ formation was marginal.

• 한국대기환경학회지
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• 제12권3호
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• pp.307-314
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• 1996

The rate of evolution of ethylent by tomato plants was rapidly increased by ozone fumigation. In the present study, the mechanism of ethylent evolution by ozone was investigated in experiments with aminoethoxyvinylglycine (AVG) and tiron, which inhibit the formation of ethylene and peroxidation of lipids, respectively. Pretreatment with AVG significantly inhibited the ozone-induced ethylent evolution, but the treatment of plants with tiron did not inhibit. These results indicate that the induction of the evolution of ethylene by ozone involves the pathway via aminocyclopropane-1-carboxylate (ACC), while not released as a result of the peroxidation of lipids. Ozone-induced ethylent evolution was greater in dar- than light-incubated, intact tomato plants. The difference between dark- and light-ethylene evolution was examined with diuron, an inhibitor of photosynthetic electron transport. The inhibitor treatment promoted ethylent evolution. These results suggest that ethylent retention and metabolism in plants were regulated by internal $CO_2$ levels which, in turn, were controlled in large part by photosynthesis. Thus, ethylene was retained in illuminated leaf tissue under low intenal $CO_2$ concentration which may develop in a sealed container without exogenously supplied $CO_2$.

• 한국산림과학회지
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• 제69권1호
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• pp.13-18
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• 1985

잣나무의 녹엽(綠葉), 낙엽(落葉), F층(層)의 엽(葉)과 떡갈 및 굴참나무의 녹엽(綠葉)을 각각 토양(土壤)에 혼합(混合)하여 53일간 $30^{\circ}C({\pm}1)$로 항온배양(恒温培養)하는 동안 토양중(土壤中)의 무기태(無機態) 질소(窒素) 및 $CO_2$ 방출속도(放出速度)의 변화(變化)를 측정(測定)하여 다음의 결과(結果)를 얻었다. 1) 배양초기(培養初期)에는 무기태(無機態) 질소(窒素)의 유기화(有機化)로 무기태(無機態) 질소량(窒素量)의 감소(減少)가 강(強)하게 일어났고, 시간(時間)의 경과(經過)에 따라 점차 증가(增加) 하였다. 2) 혼합(混合)한 엽중(葉中)의 유기태(有機態) 질소(窒素)의 유기화속도(有機化速度)는 잣나무의 엽중(葉中) 녹엽(綠葉)에서 가장 컸으나, 굴참 및 떡갈나무의 녹엽(綠葉)보다는 작았다. 3) $CO_2$ 방출속도(放出速度)의 크기는 굴참나무녹엽(綠葉), 떡갈나무녹엽(綠葉), 잣나무녹엽(綠葉), 잣나무 낙엽(落葉), F층(層)의 잣나무엽(葉)을 혼합(混合)한 토양(土壤)의 순(順)이었고, 시간의 경과(經過)에 따라 점차 감소했다. 4) 질산태(窒酸態) 질소량(窒素量)은 점차 증가(增加)하여 배양(培養) 53일 후에, 암모니아태(態應) 질소량(窒素量)을 상회(上迴)하였다.

• 대한금속재료학회지
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• 제47권2호
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• pp.107-113
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• 2009

Molten iron with 2 mass % carbon content was decarbonized at 1823 K~1923 K by bubbling $Ar+O_2$ gas through a submerged nozzle. The reaction rate was significantly influenced by the oxygen partial pressure and the gas flow rate. Little evolution of CO gas was observed in the initial 5 seconds of the oxidation; however, this was followed by a period of high evolution rate of CO gas. The partial pressure of CO gas decreased with further progress of the decarbonization. The overall reaction is decomposed to two elementary reactions: the decarbonization and the dissolution rate of oxygen. The assumptions were made that these reactions are at equilibrium and that the reaction rates are controlled by mass transfer rates within and around the gas bubble. The time variations of carbon and oxygen contents in the melt and the CO partial pressure in the off-gas under various bubbling conditions were well explained by the mathematical model. Based on the present model, it was explained that the decarbonization rate of molten iron was controlled by gas-phase mass transfer at the first stage of reaction, but the rate controlling step was transferred to liquid-phase mass transfer from one third of reaction time.

• 한국농공학회지
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• 제40권5호
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• pp.100-108
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• 1998

Compost stability represents the state of microbiological activity and measurements of respiration either through $CO_2$ evolution or $O_2$ uptake should provide the best indication of this state. Hog manure amended with sawdust was composted in a pilot-scale reactor vessels using continuous and intermittent aeration for 3 weeks. In this study we evaluated the $CO_2$ respiration rate effect of aeration method on the reduction of $CO_2$ evolution, and investigated the stability of fresh and finished compost for plant growth. The intermittently aerated composting is a practical proposition for a very stable compost making. The $CO_2$ respiration rate in the fresh and finished compost during intermittently aerated composting was maintained from 0.3 to 1.4 and was good for use in horticulture, while the continuously aerated composting was 7 to 23 and needed more time for compost curing.

• Genomics & Informatics
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• 제19권2호
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• pp.15.1-15.7
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• 2021

The direction of evolution can estimate based on the variation among nonsynonymous to synonymous substitution. The simulative study investigated the nucleotide sequence of closely related strains of respiratory syndrome viruses, codon-by-codon with maximum likelihood analysis, z selection, and the divergence time. The simulated results, dN/dS > 1 signify that an entire substitution model tends towards the hypothesis's positive evolution. The effect of transition/transversion proportion, Z-test of selection, and the evolution associated with these respiratory syndromes, are also analyzed. Z-test of selection for neutral and positive evolution indicates lower to positive values of dN-dS (0.012, 0.019) due to multiple substitutions in a short span. Modified Nei-Gojobori (P) statistical technique results also favor multiple substitutions with the transition/transversion rate from 1 to 7. The divergence time analysis also supports the result of dN/dS and imparts substantiating proof of evolution. Results conclude that a positive evolution model, higher dN-dS, and transition/transversion ratio significantly analyzes the evolution trend of severe acute respiratory syndrome coronavirus 2, severe acute respiratory syndrome coronavirus, and Middle East respiratory syndrome coronavirus.

• 한국식품과학회지
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• 제36권4호
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• pp.580-585
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• 2004

단감의 호흡에 미치는 산소, 이산화탄소 가스조성과 저장온도의 영향을 조사하기 위하여 비경쟁억제 효소반응속도식$(R=V_m[O_2]/(K_m+(1+[CO_2]/K_i)[O_2]))$과, Arrhenius 식(R=A exp(-E/$(R^*T)$)을 각각 모델로 하였다. 호흡 data는 0, 5, $20^{\circ}C$에서 폐쇄계방법으로 수집하였다. 0, $5^{\circ}C$에서 $K_m$은 0.1%이하, $K_i$는 100%이상이었고, $20^{\circ}C$에서 산소소비와 이산화탄소 발생의 $K_m$은 각각 10.72%와 3.25%로 크게 증가하였고, $K_i$는 각각 59.6%와 44.6%로 크게 감소하였다. 활성화에너지는 산소농도가 낮아지고 이산화탄소 농도가 높아질수록 감소하였고, 산소소비의 활성화에너지가 이산화탄소 발생의 활성화에너지보다 낮았다. 이는 이산화탄소 발생 호흡량이 산소소비 호흡량에 비해 온도의 영향을 많이 받고, 산소감소와 이산화탄소 증가에 따른 호흡량 감소 효과는 저온에 비해 고온에서 커지는 경향이었다. 이는 산소소비와 이산화탄소 발생의 $K_m$과 $K_i$값 비교에 의한 예측과 일치하는 결과이다. 이상의 간을 근거로 하여 각 온도별 MA포장 내 공기조성 변화의 예측하였고, 또한 실제 실험으로 조사된 값은 일치하였다. 따라서 단감의 최적 MA 포장조건설정에 있어서 효소반응속도론에 근거한 호흡모델이 타당한 것으로 판단되었다.

• KSBB Journal
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• 제5권1호
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• pp.25-35
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• 1990

In the Xanthan gum fermentation by Xanthomonas campestris there are problems of the large energy consumption by long fermentation time, the mass transfer of oxygen and nutrients by high viscous fermentation broth. In this study, the media optimization and the fed batch fermentation were carried out to decrease fermentation time and increase Xanthan gum yield. The $O_2$ uptake rate (OUR) and $CO_2$ evolution rate(CER) which were obtained from the analysis of fermentation exit gas using a gas chromatograph were investigated. As a result, the fermentation time decreased at optimal assimilable nitrogen concentration but increased at poor or rich assimilable nitrogen concentration, the Xanthan gum biosynthesis was stimulated under the limited condition of assimilable nitrogen source and the optimum fermentation medium was obtained as follow; Glucose=30g / l, Peptone=8.0g / l, $K_2HPO_4=2.0g/l$, $MgS0_47H_2O=10g/l$, Sodium acetate=20g/l, Sodium pyruvate=0.5g/1. As the agitation speed and nitrogen concentration increased, the $O_2$ uptake rate and $CO_2$ evolution rate increased. The OUR and CER were 37.3mmol $O_2/\;l$ hr and 20.2 mmol $CO_2/\;L$ hr at peptone 11g / l and agitation speed 990RPM, respectively. In fed batch fermentation, the final concentration of Xanthan gum was enhanced up to 29g / l.