• Korean Journal of Food Science and Technology
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• v.32 no.1
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• pp.161-167
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• 2000

The protease produced by a newly isolated Aspergillus wentti from Korean traditional Meju was purified and characterized. The optimal medium composition and culture conditions for maximum protease production were ; bran :1% glucose solution =1 : 1, pH 9.0, $30^{\circ}C$, and 4 days of fermentation. Protease was purified by QAE-Sephadex, SP-Sephadex ion exchange chromatography and Sephadex G-100 chromatography. The specific activity and the purification fold of the purified enzyme were 213 unit/mg protein and 27.3, respectively. The molecular weight of purified protease was found to be 32 kDa by SDS-PAGE. Km and Vmax value's for hammastein milk casein were $3.049{\times}10^{-4}\;M\;and\;151.1\;{\mu}g/min$, respectively. Kinetic parameters showed that the enzyme has higher affinity to casein than isolated soybean protein, hemoglobin and bovine serum albumin. Optimal pH and temperature for reaction of the purified enzyme were 9.0 and $50^{\circ}C$, respectively. The enzyme was stable at pH 4.0-11.0, below $40^{\circ}C$, and the activity was not stimulated by metal ions. 1mM phenylmethylsulfonyl fluoride inhibited the enzyme activity by 98.5%. It means that the enzyme is one of serine protease.

• Journal of Information Technology Services
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• v.19 no.3
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• pp.57-73
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• 2020

The proportion of digital evidence in criminal cases has increased, while at the same time, the spread of the Internet has made it easy to delete information that is stored in another place and thus, the Internet is being used to delete online criminal evidence. To respond quickly and effectively to cybercrime, 29 countries signed the Convention on Cybercrime in 2001 through the Council of Europe. Article 16 of the Convention relates to the expedited preservation of stored computer data and requires signatories to adopt legislative measures to enable its competent authorities to order expeditious preservation of specified computer data where there are grounds to believe that the data is particularly vulnerable to loss or modification. More than 60 countries have joined the Convention since 2001 and have made efforts to improve their legal system in line with it. The United States legislated 18 U.S.C. § 2703(f) to preserve electronic evidence pending the issuance of a court order. The German Code of Criminal Procedure §§ 94~95 allows prosecution authorities to seize evidence or issue production orders without court control in urgent circumstances. A custodian shall be obliged to surrender evidence upon a request that evidence be preserved, and non-compliance results in punishment. Japan legislated the Criminal Procedure Act § 197(3) and (4) to establish a legal base for requesting that electronic records that are stored by an ISP not be deleted. The Korean Criminal Procedure Act § 184 outlines procedures for the preservation of evidence but does not adequately address the expeditious preservation of digital evidence that may be vulnerable to deletion. This paper analyzes nine considerations, including request subjects, requirements, and cost reimbursement to establish directions to improve the legal system for the expedited preservation of digital evidence. A new method to preserve online digital evidence in urgent cases is necessary.

• Journal of Broadcast Engineering
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• v.11 no.3 s.32
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• pp.320-325
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• 2006

An image is partitioned into non-overlapping $8{\times}8$ blocks fer JPEG compression. A scan order converter is placed before the JPEG encoder to provide $8{\times}8$ blocks from the pixels in raster scan order. In general, its architecture requires two line memories for storing eight lines separately to allow the concurrent memory access by both the camera and JPEG processors. Although such architecture is simple to be implemented, it can be inefficient due to too excessive memory requirement as the image resolution increases. However, no deterministic addressing equation has been developed for scan conversion. In this paper, an effective memory addressing algorithm is proposed that can be devised only by adders and subtracters to implement a scan converter based on the single line memory.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.48 no.3
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• pp.62-70
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• 2011

Nowadays image sensor is an essential component in many multimedia devices, and it is covered by a color filter array to filter out specific color components at each pixel. We need a certain algorithm to combine those color components reconstructed a full color image from incomplete color samples output from an image sensor, which is called a demosaicking process. Most existing demosaicking algorithms are developed for ideal image sensors, but they do not work well for the practical cases because of dissimilar characteristics of each sensor. In this paper, we propose a new demosaicking algorithm in which the color filter characteristics are fully utilized to generate a good image. To demonstrate significance of our algorithm, we used a commerically available sensor, CBN385B, which is a sort of Honeycomb-style CFA(Color Filter Array) CCD image sensor. As a performance metric of the algorithm, PSNR(Peak Signal to Noise Ratio) and RGB distribution of the output image are used. We first implemented our algorithm in C-language for simulation on various input images. As a result, we could obtain much enhanced images whose PSNR was improved by 4~8 dB compared to the commonly idealized approaches, and we also could remove the inclined red property which was an unique characteristics of the image sensor(CBN385B).Then we implemented it in hardware to overcome its problem of computational complexity which made it operate slow in software. The hardware was verified on Spartan-3E FPGA(Field Programable Gate Array) to give almost the same performance as software, but in much faster execution time. The total logic gate count is 45K, and it handles 25 image frmaes per second.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.4
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• pp.516-522
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• 1998

An Actinomycetes strain, MSA-1, containing antimicrobial component was isolated from the black sponge, Halichondzia okadai, and was identified to a genus level by morphological and chemotaxonornic methods. The gray colored spores were oval type with smooth surface and formed flexibilis spore chains. The cell wall of this strain was type I containing D-aminopimellic acid (D-DAP) and no specific sugar was detected. Phospholipid of the cell membrane was PII type including phophoethanolamine and the major fatty acids of total lipid were branched anteiso-15 : 0, iso-16 : 0, 16 : 0 and iso-17 ; 0. From these results and other characteristics described in the Bergey's Manual, this strain was identificated as a Streptomyces sp. Meanwhile, 10mg of pale yellow colored antimicreobial component was isolated by HPLC method from the cultured Streptomyces sp. (70g of cryophillized mycellis). By crystallographyc analysis, HIRESMS and NMR assignment, the antimicrobial component produced from the strain MSA-1 was elucidated as the staurosporine (indolo[2,3-a]carbazole alkaloid).