• 한국식품과학회지
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• 제32권5호
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• pp.991-996
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• 2000

식품 중 대두단백질의 분석을 위한 효소면역측정법(ELISA)을 개발하고자 하였다. 대두단백질의 주 구성 성분이며 열에 안정한 glycinin의 acidic subunits(AS)에 대한 특이항체를 생산하였고 이를 이용하여 간접경합 ELISA(ciELISA)를 확립하였다. 시료처리조건은 urea와 DTT로 처리함으로써 용해시킨 다음 $100^{\circ}C$에서 1시간 열처리하였고 cystine을 함유한 용액으로 재생시켰다. 이러한 시료의 처리조건하에서 ISP를 $60-90^{\circ}C$까지 열처리한 시료에 대해서도 분석결과의 변화가 거의 없는 것으로 나타났다. 표준곡선 상에서 ISP의 검출한계는 0.3 ${\mu}g/mL$이었다. 항AS 항체의 다른 단백질에 대한 반응성을 검토한 결과, 탈지분유, 카제인, 난백분말, ovalbumin에서 0.1%이하로 미약하게 반응하거나 거의 반응하지 않는 것으로 나타났다. ISP를 0.5-2%첨가한 탈지분유에 대한 ISP 분석 회수율은 평균 76%(C.V., 11.5%)였으며 ISP를 0.5-3% 첨가하여 시험 제조한 소세지의 경우 평균 83%(C.V., 19%)로 나타났고 시판 소세지 6점에 함유된 ISP의 함량은 평균 1.27%로 나타났다.

• 한국추진공학회지
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• 제15권3호
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• pp.1-8
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• 2011

고농도 과산화수소를 이용하는 1,200 N 급 이원추진제 로켓 엔진 개발을 위한 기존 연구와 더불어, 특성길이의 영향 및 추력 측정을 통한 실질적인 성능을 평가하였다. 특성길이는 0.95, 1.07과 1.20 m, 총 3가지 경우에 대하여 실험을 수행하였으며, 특성길이의 증가에 따라 $C^*$ 효율 및 Isp 효율 모두 증가함을 확인하였다. 설계 당량비에서의 최대 $C^*$ 및 Isp 효율은 각각 98.4%와 93.1%로 측정되었다. 엔진성능 평가 결과를 바탕으로 분해된 과산화수소를 이용한 엔진에서의 최적 특성길이를 제안하고, 설계 당량비에서의 추력 및 비추력 효율을 이용하여 진공에서의 엔진성능을 예측하여 보았다. 그 결과, 지상 218.4 s, 진공 253.3 s의 비추력과, 진공 추력 1035.3 N의 성능을 예상할 수 있다.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2010년도 제35회 추계학술대회논문집
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• pp.55-62
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• 2010

고농도 과산화수소를 이용하는 1,200 N 급 이원추진제 로켓 엔진 개발을 위한 기존 연구와 더불어, 특성길이의 영향 및 추력 측정을 통한 실질적인 성능을 평가하였다. 특성길이는 0.95, 1.07과 1.20 m, 총 3가지 경우에 대하여 실험을 수행하였으며, 특성길이의 증가에 따라 $C^*$ 효율 및 Isp 효율 모두 증가함을 확인하였다. 설계 당량비에서의 최대 $C^*$ 및 Isp 효율은 각각 98.4%와 93.1%로 측정되었다. 엔진 성능 평가 결과를 바탕으로 분해된 과산화수소를 이용한 엔진에서의 최적 특성길이를 제안하고, 설계 당량비에서의 추력 및 비추력 효율을 이용하여 진공에서의 엔진성능을 예측하여 보았다. 그 결과, 지상 218.4 s, 진공 253.3 s의 비추력과, 진공 추력 1035.3 N의 성능을 예상할 수 있다.

• 대한미생물학회지
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• 제21권2호
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• pp.251-259
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• 1986

This study was performed to assess the effect of inosiplex(ISP) on the resistance of mice Candida albicans infection, the migration of chicken leukocytes, the production of leukocyte migration inhibitory factor(LIF), and the cell-mediated immunity(CMI) to lepomin in multibacillary lepromatous leprosy patients. The treatment with ISP before or on the time of infection with C. albicans had no or deliterious effect, and treatment with ISP after infection had no effect on the recovery of C. albicans from the kidneys of mice. The migratory ability of chicken leukocytes and the production of LIF from splenocytes of mice were not affected by ISP treatment. However, ISP decreased the migration of chicken leukocytes in vitro, and this decrease was dose-dependent. The therapy of lepromatous leprosy patients with ISP for 10 or 30 days clearly showed the increase of the significant positive rate of Mitsuda skin test to lepromin. The immune recovery as a result of the therapy was found to be the best in the group of patients treated for 30 days. This results suggest that (1) the effect of ISP in renal candidiasis can vary depending on the time of treatment relative to infection, (2) ISP can primarily change the migratory ability of chicken leukocytes but does not affect the production of LIF in mice, and (3) the classical therapy combined with ISP can reinforce or restore the defences of lepromatous leprosy patients against Mycobacterium leprae.

• BMB Reports
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• 제40권6호
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• pp.911-920
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• 2007

Tuberculosis, caused by Mycobacterium tuberculosis, continues to be one of the leading infectious diseases to humans. It is urgent to discover novel drug targets for the development of antitubercular agents. The 2-C-methyl-Derythritol-4-phosphate (MEP) pathway for isoprenoid biosynthesis has been considered as an attractive target for the discovery of novel antibiotics for its essentiality in bacteria and absence in mammals. MEP cytidyltransferase (IspD), the third-step enzyme of the pathway, catalyzes MEP and CTP to form 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME) and PPi. In the work, ispD gene from M. tuberculosis H37Rv (MtIspD) was cloned and expressed. With N-terminal fusion of a histidine-tagged sequence, MtIspD could be purified to homogeneity by one-step nickel affinity chromatography. MtIspD exists as a homodimer with an apparent molecular mass of 52 kDa. Enzyme property analysis revealed that MtIspD has high specificity for pyrimidine bases and narrow divalent cation requirements, with maximal activity found in the presence of CTP and $Mg^{2+}$. The turnover number of MtIspD is $3.4 s^{-1}$. The Km for MEP and CTP are 43 and $92{\mu}M$, respectively. Furthermore, MtIspD shows thermal instable above $50^{\circ}C$. Circular dichroism spectra revealed that the alteration of tertiary conformation is closely related with sharp loss of enzyme activity at higher temperature. This study is expected to help better understand the features of IspD and provide useful information for the development of novel antibiotics to treat M. tuberculosis.

• 한국감성과학회:학술대회논문집
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• 한국감성과학회 2003년도 추계학술대회 논문집
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• pp.1221-1222
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• 2003

본 연구는 Usability 중심의 웹사이트 평가를 위한 프레임워크 모델의 구축을 목표로 한다. 이를 위해 기존에 구축된 웹사이트 평가 방법론을 분석하여 평가 요소 별 중요도를 설정하였다. 비교 분석을 통해 나온 문제점들을 기초로 웹사이트평가를 위한 평가요인(Critical Factor)을 선정하여 새로운 프레임워크 모델 $C^3$ISP(Contents, Community, Commerce, Interface, Security, Performance)를 제시하였다. 향후 연구과제로는 프레임워크의 유용성을 분석하기 위한 Running Test를 실시한다.

• Journal of Pharmaceutical Investigation
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• 제23권3호spc1호
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• pp.41-50
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• 1993

The iontophoretic delivery across rat skin of quaternary ammonium salts (isopropamide: ISP, pyridostigmine: PS), which are positively charged over a wide pH range, was measured ill vitro. The study showed that: (a) iontophoresis significantly enhanced delivery of ISP and PS compared to respective passive transport; (b) delivery of ISP and PS was directly proportional to the applied continuous direct current density over the range of $0-0.69\;mA/cm^2;$ (c) delivery of ISP and PS was also proportional to the drug concentration in the donor compartment over the range of $0-2{\time}l0^{-2}M:$ (d) sodium ion in the donor compartment inhibited the drug transport possibly due to decreasing the electric transference number of the drug; (e) delivery of ISP and PS increased as the pH of the donor solution increased over the pH range 2-7 suggesting permselective nature of the epidermis, and inhibition of the transference number of the drugs by hydronium ion; (f) some organic anions such as taurodeoxycholate, salicylate and benzoate which form lipophilic ion-pair complexes with ISP inhibited the delivery of ISP. The degree of inhibition by the organic anions was linearly proportional to the extraction coefficient $(K_e)$ of ISP from the partition system with each counteranion between phosphate buffer (pH 7.4) and n-octanol. For PS, however, taurodeoxycholate, but not salicylate and benzoate inhibited the iontophoretic delivery. It suggests that not only sodium ion and hydronium ion but also the counteranions which form lipophilic ion-pairs with quaternary ammonium drugs are not favorable components in formulating the donor solution of the drugs to achieve an effective iontophoretic delivery.

• Journal of Microbiology
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• 제35권1호
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• pp.40-46
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• 1997

The pWHM220 cosmid with a 24-kb insert cloned from Streptomyces albus ATCC 21838 induces the biosynthesis of a polysther antibiotic similar to salinomycin in Streptomyces invidans. We have analyzed this region by DNA sequencing as well as Southern blot hybridization with type I and type II polyketide synthase (PKS) probes. Surprisingly, we found another set of type II SKS genes only 10-kb from the original PKS genes, salABCDE. The DNA sequence revealed two complete open reading frames (ORFs) named salB2 and salC2, and one partial ORF that does not resemble any known DNA or deduced protein sequence. The salC2 should code for chain length determining factor while the deduced amino acid sequence encoded by salB2 exhibits high similarity to .betha.-ketoacyl synthase from different PKS gene clusters. The highest identity was found for .betha.-keetoacyl synthases from S. argillaceus (MtmP. 59.1% identity), the mithramycin producer and from S. venezuelae ISP5230 (JadA, 52.3% identity), the jadomycin producer. The SalC2 protein clearly resembles its counterparts in order aromatic PKS gene clusters that are believed to influence the length of the polyketide chain. The highest identities observed were to that of S. argillaceus (MtmK, 62.3%) and S. venezuelae ISP 5230 (JadB, 55.1%) proteins, Moreover, the deduced amino acid sequences of the salB2 and salC2 products were 29.0% identical.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 1998년도 춘계학술발표회논문집(1)
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• pp.629-634
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• 1998

프랑스 CEA 실험장치인 BETHSY 실험설비에서 수행된 부분충수 운전에 대한 실험인 6.9c 실험에 대한 MELCOR 1.8.3 의 평가를 수행하였다. 이 연구는 OECD/NEA 국제공동연구인 ISP-38 로 수행되었다. 평가결과, MELCOR 1.8.3 은 부분충수운전시 잔열제거계통 상실에 대한 예측능력이 있고 원자로 냉각재계통압력, 노심수위 등 전반적으로 거동을 잘 모의하고 있다고 판단되었다. 그러나 민감도 분석에서 도출된 결론에 의하여 상간의 운동량 전달 및 Liquid Entrainment모델에 있어서 개선 필요성이 있는 것으로 평가되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제14권9호
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• pp.1316-1320
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• 2001

The objectives of this study were to investigate the quality characteristics of restructured pressed smoked duck steak from the breast meat of Cherry Valley ducks. Different levels of isolated soybean protein (ISP) (0, 15 and $30g{\cdot}kg^{-1}$) or carrageenan (5, 10 and $15g{\cdot}kg^{-1}$) were added to manufacture the restructured pressed smoked duck steak. The results were as follows: No significant differences were observed for moisture, crude fat, crude protein, cooking loss and water holding capacity of products from all treatments. The panel test scores showed that color, flavor and binding ability of products were considered acceptable. The drip loss in control sliced-products was significantly higher than products containing ISP or carrageenan (p<0.05) during storage at $-18^{\circ}C$ for 12 weeks. The pH value, volatile basic nitrogen (VBN) value and thiobarbituric acid (TBA) value of vacuum-packaged products did not change significantly during storage at $-18^{\circ}C$ for 6 weeks. However, TBA values increased with storage time. The viable bacterial counts were about $10^{3}-10^{4}CFU/g$ during storage at $-18^{\circ}C$ for 12 weeks. The products remained good quality during the storage period.