• Journal of Acupuncture Research
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• v.33 no.2
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• pp.89-96
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• 2016

Objectives : The aim of this study is to investigate the needling depth of Hwangmun (BL51) and Jisil (BL52) retrospectively using an L-spine MRI. Methods : We measured the shortest distance from the skin to transversalis fascia at both sides of Hwangmun and Jisil, and analyzed the difference between male and female using a student t-test, and between the left and right sides with a paired t-test. Results : In the case of males, the average depth of left Hwangmun was $48.24{\pm}10.16mm$, and that of right was $47.23{\pm}9.59mm$ ; left Jisil was $56.91{\pm}9.00mm$ and right was $55.74{\pm}8.75mm$. In the case of females, the average depth of left Hwangmun was $42.26{\pm}9.29mm$, and that of right was $41.63{\pm}9.32mm$ ; left Jisil was $49.21{\pm}10.77mm$ and right was $48.41{\pm}11.38mm$. The depth of male insertion was deeper than that of female in Jisil, but there was no significant difference according to gender in Hwangmun. Conclusion : For males, the needling depth of left Hwangmun is 37.21 ~ 68.23 mm and right is 36.91 ~ 70.50 mm, while left Jisil is 42.97 ~ 70.84 mm and right is 43.75 ~ 72.00 mm. For females, the needling depth of left Hwangmun is 26.80 ~ 68.28 mm and right is 25.70 ~ 65.59 mm, while left Jisil is 30.94 ~ 79.06 mm and right is 28.13 ~ 77.27 mm.

• Korean Journal of Acupuncture
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• v.36 no.3
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• pp.150-161
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• 2019

Objectives : The authors aimed at contributing to the safety of acupuncture in the clinic by analyzing the information of contraindicated acupoints described in Uihakipmun. Methods : We investigated the contents related acupuncture contraindications in Uihakipmun, categorized the contraindicated acupoints by site and identified whether they can cause adverse events from the anatomical point of view. Results : In Uihakipmun, 49 acupoints - BL6, BL8, BL9, ST1, SI18, BL1, BL2, TE18, TE19, TE20, GB1, GB3, GV22, GV24, ST12, ST9, TE16, GV6, BL15, BL30, CV17, LU2, GB22, SP16, CV8, CV9, CV15, KI11, ST30, SP11, BL56, GB32, GB33, GB42, LI13, HT2, TE8, GV17, GB18, EX-HN11, GB21, GV11, GV10, ST17, CV5, CV1, ST42, SP6, LI4 - were described as contraindicated acupoints. Among them, the contraindications induced by acupuncture treatment were described for just 5 acupoints. Anatomically, acupuncture stimulation at the contraindicated acupoints can cause organ, nerve or vessel damage near the acupoints. And the number of contraindicated acupoints in Uihakipmun was increased compared to those in Hwangjenaegyeong, Chimgugabeulgyeong, Bigeupcheongeumyobang, Donginsuhyeolchimgudogyeong and Chimgujasaenggyeong. Conclusions : In Ming dynasty, the knowledge associated with adverse events on acupuncture was increased by the accumulation of medical knowledge. Acupuncture stimulation at the contraindicated acupoints described in Uihakipmun can cause tissue damage, therefore we should perform acupuncture procedure carefully to avoid adverse events when stimulating the contraindicated acupoints.

• Journal of Pharmacopuncture
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• v.5 no.1 s.8
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• pp.135-151
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• 2002

Objective: This study was eanied out to investigate the effects of Raphani Semen on immuno-response in the mouse model of allergic asthma. Methods: In this study, BALB/C mice were divided into 6 groups: Normal (Non-treated group), Control (Group with not treated after allergic sensitization and induction by ovalbumin), Treat I (Group with the oral administration of saline after allergic sensitization and induction by ovalbumin), Treat n (Allergic asthma group treated with acupuncture (BL 13)), Treat III (Allergic asthma group treated with the oral administration of Raphani Semen) and Treat lV (Allergic asthma group treated with the herbal-acupuncture of Raphani Semen (BL 13)). The effect on cytokine was assessed by measuring cytokine (lL-2, IL-4, IL-5, IL-10, IL-12, IFN-r) in bronchoalveoar lavage fluid(ELISA). ResuJts : The results obtained as follows: 1. The production of Interleukin-2 was decreased significantly in Treat I group, Treat n group and Treat IV group as compared with Control group. 2. The production of Interleukin-4 was decreased significantly in Treat I group, Treat II group and Treat IV group as compared with Control group. Among them. the production of Interleukin-4 was decreased remarkably in Treat IV group as compared with other groups. 3. The production of Interleukin-5 was decreased significantly in Treat I group and Treat IV group as compared with Control group. 4. The production of Interleukin-10 was decreased significantly in Treat I group and Treat III group as compared with Control group. 5. The production of Interleukin-12 was all decreased significantly in Treat I group, Treat n group, Treat m group and Treat IV group as compared with Control group. 6. The production of Intelferon- showed no significant changes in Treat I group, Treat n group. Treat m group and Treat Ⅳ group as compared with Control group. Conclusion: These results show that the production of Interleukin-4, 5 was decreased significantly in aJlergic asthma group treated with the herbal-acupuncture of Raph Semen (BL 13), It is known that inactivity of Th2 cell constrained the revelation and controlled hypersenstive action. As to this mechanism, it is suggested that the herbal-acupuncture of Raphani Semen(BL 13) constrained the revelation of allergic asthma.

• Applied Biological Chemistry
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• v.48 no.3
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• pp.201-206
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• 2005

The development of microbial inoculant was conducted using a by-product of oriental herbal medicine. The constituent of the by-product, which was high in organic matter, was 11.3% of crude protein, 5.1% of crude lipid, 49.7% of NDF (neutral detergent fiber), and 33.8% of ADF (acid detergent fiber). Microorganisms isolated from the by-product of oriental herbal medicine were 35 species. Among them, 6 bacterial species, 4 fungal species, 2 actnomycetes species, and 1 yeast species were effective in the utilization of the by-products. The 13 strains screened were tested for the plant growth-promoting effect in soybean seedling. BL-333 strain was found to increase the soybean yield by about 23% as compared with control. The strain BL-333 was identified as Paenibacillus marcerans. P. marcerans BL-333 showed high anti-fungal activities against virulent fungi, especially Fusarium sp. and Collectotrichum sp. Yields of plants which were inoculated with microbial inoculant prepared with P. marcerans BL-333 and by-product of oriental herbal medicine were found to be higher than control by $3{\sim}24%$. The yield was especially promoted in lettuce, radish, chinese cabbage and cucumber plants.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.626-632
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• 2005

A new bacterium BL-2 excreting a novel cationic polyglucosamine biopolymer was isolated from the spoiled leaves of Chinese cabbage and identified as Enterobacter sp. BL-2. The isolated Enterobacter sp. BL-2 was cultivated in pH-stat fed-batch culture using acetic acid as the feeding stock at pH 8.0, resulting in 17.11 g/l of cells and 1.53 g/l of an extracellular biopolymer after 72 h. The excreted biopolymer was purified by a three-step procedure, involving ethanol precipitation and deproteinizations, to a nearly homogeneous state, and its molecular weight was found to be 106 kDa. It was composed of glucosamine, rhamnose, and galactose at a molar ratio of 86.4:1.6:1.0, respectively, indicating a rarely found novel high-glucosamine-containing biopolymer. The FT-IR and $^{13}C-NMR$ spectra of the novel cationic polyglucosamine biopolymer PGB-l revealed a close identity with chitosan from crab shell. It can effectively flocculate various suspended solids, including kaolin clay, $Ca(OH)_2,\;Al_{2}O_3$, active carbon, microbial cells, and acidic dyes.

• Korean Journal of Pharmacognosy
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• v.44 no.1
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• pp.30-40
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• 2013

The essential oils of stems, roots, fruits and leaves of Lindera obtusiloba BL. were collected in the winter and summer extracted by simultaneous distillation extraction (SDE) apparatus and analyzed by gas chromatography-mass spectrometry (GC-MS). In present study, 58 kinds of volatile components in the winter stems (WS), 70 in the winter roots (WR), 77 in the summer stems (SS), 78 in the summer roots (SR), 70 in the summer fruits (SF) and 76 in the summer leaves (SL) were identified. The results showed that, the major components were monoterpenes including ${\alpha}$-thujene (1.22~13.80%) camphene (1.56~18.40%), ${\beta}$-mycrene (1.75~9.27%), limonene (5.57~12.83%), ${\beta}$-phellandrene (3.03~7.72%), linalyl acetate (2.29~12.55%), dihydromycrene (0~11.15%), germacrene B (0~7.54%) of which the contents had major fluctuations in different seasons and parts. In general, monoterpenes were the major constituent of SF in L. obtusiloba BL. that have presented possibilities for industrial applications.

• Korean Journal of Acupuncture
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• v.27 no.4
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• pp.97-109
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• 2010

Objective : To examine whether any correlation between tendency towards depression and tenderness at special acupuncture points exists, thus to explore the potential diagnostic property of acupuncture points. Methods : A total 31 subjects were included in this study. They filled out questionnaires about their mental [Beck Depression Inventory (BDI), Stress Response Inventory (SRI), Profile of Mood States (POMS)] and physical (fatigue due to overexertion, and food accumulation) symptoms. Identical weight around Alarm points (CV17, CV12, ST25, CV5, CV4, and LR13) and transport points (BL14, BL20, BL21, BL22, BL25, and BL27) was given using an algometer and the subjects rated their pain on an 11-point numerical rating scale. Heart rate variability (HRV) was also measured. Results : The subjects were divided into two groups, normal and depressive tendency groups with a cut-off point of nine on BDI. The depressive tendency group reported significantly higher values in SRI, POMS, and questionnaire for fatigue due to overexertion. In the pressure pain measurement, depressive tendency group had more pressure pain at CV12, left side of BL20, BL14, BL22 and both sides of BL21, BL25, BL27, significantly (each p<0.05). The data of HRV did not show significant differences between groups. Conclusions : People with a tendency towards depression may be prone to stress, negative mood, and fatigue due to overexertion. In addition, they may be more likely to develop tenderness at alarm points and transport points compared with healthy people. Further research is needed to confirm this finding.

• Journal of Korean Medicine for Obesity Research
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• v.13 no.1
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• pp.17-23
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• 2013

Objectives: This study was performed to evaluate the effects of fermented Samjung-hwan (SJH) extracts on weight, serum lipids and blood glucose. Methods: SJH was fermented using three different probiotic bacterial strains (Lactobacillus plantarum [LP], Leuconostoc mesenteroides [LM], Bifidobacterium longum [BL]) separately. Thirty-six rats were divided into normal, control (high fat diet), SJH-UF (high fat diet+unfermented SJH 200 mg/kg), SJH-LP (high fat diet+LP fermented SJH 200 mg/kg), SJH-LM (high fat diet+LM fermented SJH 200 mg/kg) and SJH-BL (high fat diet+BL fermented SJH 200 mg/kg). For 8 weeks later, we examined body weight, total cholesterol, high-density lipoprotein (HDL)-cholesterol and blood glucose. Results: The control group showed significantly increased weight gain compared with normal group and SJH-LP and BL groups had less weight gain than control group, significantly. In the lipid serum tests, control group showed significantly increased total cholesterol levels compared with normal group and only SJH-LP represented decreased total cholesterol levels compared with control group. However there was no significant change in the HDL-cholesteol levels. In the blood glucose tests, that of control group significantly incereased more than that of normal group, SJH-BL showed significantly decreased blood glucose levels compared with control group. Conclusions: SJH-LP, SJH-BL showed weight control effect, SJH-LP decreased TC and SJH-BL reduced blood glucose.

• Journal of Convergence Korean Medicine
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• v.4 no.1
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• pp.13-17
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• 2022

Objectives: Primary dysmenorrhea is a representative disease that causes intestinal pain, and it has long been known that acupuncture treatment is effective. In this study, a network analysis was conducted using acupuncture points reported in the existing systematic literature review, and the combination of acupuncture points used to treat primary dysmenorrhea was analyzed. Methods: Among of a total of 60 papers, excluding those that used ear acupuncture, the analysis results indicated that combinations of BL23, BL40, BL25, BL60, BL30, Ashi-points, BL26, GB34, and GV3 were used in combination. Results: Through this, it can be seen that previous studies to reveal the effectiveness of acupuncture on back pain mainly used the acupoints of the bladder meridian (BL), local acupoints group, and distal acupoints group. Conclusion: In order to reveal the correlation between acupoints and therapeutic effects through future studies and to provide guidelines on the principles of selecting and combining acupoints, it is necessary to employ a quantitative analysis method for therapeutic effects and to provide detailed reports on acupoints.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.4
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• pp.1115-1118
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• 2012

Diallyl sulfide (DAS), a flavoring compound derived from garlic, is considered to have cancer chemopreventive potential in experimental animals and humans. This study was designated to examine possible chemopreventive effects of DAS on colon carcinogenesis using genetically engineered transgenic $Apc^{Min/+}$ mice, a well-established animal model for familial adenomatous polyposis (FAP) and sporadic colorectal cancer. Male C57BL/6J-$Apc^{Min/+}$ mice were divided into three groups. Animals of group 1 were placed on the basal diet (AIN-76A) as non-treated controls. Animals of groups 2 and 3 were given DAS-containing diets (in doses of 100 and 300 ppm, respectively). All mice were sacrificed at the end of week 10 of the experiment. Histopathological investigation revealed that the incidence of colonic polyps was decreased dose-dependently by 19% (13/16) in group 2 and by 32% (13/20) in group 3 compared to the 100% incidence (10/10) in group 1. The multiplicity of colonic polyps per mouse was also slightly decreased by DAS treatment ($1.88{\pm}0.35$ in group 2 and $1.63{\pm}0.36$ in group 3) compared to $2.00{\pm}0.39$ in group 1. On the other hand, there were no significant differences in the numbers of total polyps per mouse in the small intestine between the groups. Taken together, we suggest that DAS may exert promising inhibitory effects on colon carcinogenesis in the transgenic $Apc^{Min/+}$ mice.