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Studies on Growth Stimulation of Lactic Starter (유산균(乳酸菌) Starter의 생육촉진(生育促進)에 관(關)한 연구(硏究))

  • Kim, Jong Woo;Lee, Young Soo
    • Korean Journal of Agricultural Science
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    • v.19 no.1
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    • pp.40-50
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    • 1992
  • This experiment was carried out to stimulate lactic starter culture for yoghurt manufacturing. A each of 1.5% of Bios 2000, CR starter medium, and Yeast extract were added to bulk medium, acidity, pH and changes in the number of lactic acid bacteria were investigated at, intervals of two hours for Lactobacillus bulgaricus and four hours for Streptococus thermophilus and Lactobacillus casei. The results obtained were summarized as follows. 1. The acidity of control arrived at 0.99% after 16 hours of incubation during the incubation of Lactobacillus bulgaricus. Whereas that of CR starter medium reached 1.00% at 12 hours of incubation. Yeast extract, 1.12% at 12 hours, and Bios 2000 reached 0.97% at 10 hours respectively, Thus, Bios 2000 showed the fastest rate of acid production. 2. When the acidity of experiment medium peaked on optimum levels. pH of control was 4.03 in 16 hours of incubation during the incubation of Lactobacillus bulgaricus. Whereas that of Bios 2000 reached 4.10 of Yeast extract reached 3.97 at 12 hours, and of CR starter medium reached 4.05 at 12 hours. 3. Lactic acid bacterial counts were $3.1{\times}10^{10}/ml$ after 16 hours of incubation during the incubation of lactobacillus bulgaricus, Whereas those of Bios 2000 reached $2.1{\times}10^{10}/ml$ at 10 hours, with the fastest stimulation of growth, The counts in CR starter medium were at $2.9{\times}10^{10}/ml$ at 12 hours, and Yeast extract were $3.8{\times}10^{10}/ml$ at 12 hours. 4. The acidity of control, CR starter medium, and Yeast extract reached 0.92% at 44 hours, and 0.96% at 32 hours, and 0.90% at 32 hours respectively, Also, that of Bios 2,000 reached 0.97% at 32 hours, which exhibited the highest, among the treatments. 5. The pH of control was 4.27 at 44 hours. that of CR starter medium was 4.33 at 40 hours and that of Yeast extract was 4.25 at 32 hours during the incubation in Streptococcus thermophilus. Besides, pH of Bios 2000 is lowest as 4.18 at 32 hours. 6. Lactic acid bacterial counts in control, CR starter medium, and Yeast extract during the incubation of Streptococcus thermophilus were $9.8{\times}10^{9}/ml$ at 44 hours,$9.5{\times}10^{8}/ml$ at 40 hours, and $9.6{\times}10^{8}/ml$ at 32 hours. And, the highest number was $2.0{\times}10^{9}/ml$ for Bios 2000 at 32 hours. 7. The acidity of control during the incubation of Lactobacillus casei reached 0.92% at 40 hours, and those of CR starter medium and Yeast extract were 0.95% at 40 hours, and 1.01% at 36 hours respectively. Also, Bios 2000 had the highest acidity as 0.94% at 32 hours. 8. The pH of control, CR starter medium and Yeast extract during the incubation Lactobacillus casei was 4.27 at 40 hours. 4.21 at 40 hours, and 4.15 at 36 hours respectively. Also, Bios 2000 showed the lowest pH, as 4.23, at 32 hours. 9. Lactic acid bacterial counts in control, CR starter medium and Yeast extract during the incubation of Lactobacillus casei were $9.4{\times}10^{7}/ml$ at 40 hours, $1.1{\times}10^{8}/ml$ at 40 hours, and $5.0{\times}10^{8}/ml$ at 36 hours respectively. And, the progress of 32 hours showed the highest number of lactic acid bacteria as$6.4{\times}10^{8}/ml$ in Bios 2000.

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A Study on the Foaming Properties of Small red bean Protein Isolates at Various Conditions (분리 팥 단백질의 기포 특성에 영향을 주는 제 요인에 관한 연구)

  • 김현정
    • Journal of the Korean Home Economics Association
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    • v.28 no.2
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    • pp.37-45
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    • 1990
  • The purpose of this study was to determine the foaming properties of two small red bean protein isolates at various conditions. Data concerning the effects of pH, temperature, MaCl concentration, sugar concentration and protein concentration on the properties such as solubility, foam expansion, foam stability were presented. The results were summarized as follows : 1. The crude protein contents of two small red beans were 26.14% and 22.71%. The percentage of nonpolar amino acid group was the highest and that of sulfur containing amino acid group was the lowest. 2. Protein solubility showed the minimum at pH 4.5 which is isoelectric point of small red bean protein isolate adn heat treatment lowered solubility(P<0.05). At pH 4.5, solubility increased sighificantly as 0.4M NaCl was added. However, the effect of sugar concentration in the solubility was not significant. 3. Foam expansion of two small red bean protein isolates was high at pH 4.5 and heat treatment at 10$0^{\circ}C$ lowered foam expansion(P<0.05). While addition of NaCl, sugar did not affect the foma expansion, gradual increment of the protein isolates concentration up to 9% decreased the foma expansion slightly. 4. Foam stability was significantly high at pH 4.5 and heat treatment at 10$0^{\circ}C$ lowered foam stability. Addition of sugar caused slight decrease in foam stability. From 1% to 9% suspension, foma stability increased significantly as protein concentration increased(P<0.05)

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Biochemical and Physiological Properties of Fermented Skate (홍어 숙성과 기능성)

  • 최명락;유은정;임현수;박재원
    • Journal of Life Science
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    • v.13 no.5
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    • pp.675-683
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    • 2003
  • This study was conducted to investigate the physiological properties of various parts of skate body after fermentation by measuring compositional properties including pH and $NH_4^+$. Other functional properties, such as antibacterial activities, antioxidative activities and anticancer activities were measured. Effects of fermentation temperature (4, 10, $20^{\circ}C$) did not affect compositional properties of fermented skate. The pH of fermented skate extract at 4$^{\circ}C$ did not increase as much as that at 10 and $20^{\circ}C$, Particularly at $10^{\circ}C$, the pH increased rapidly after Day 1 and remained unchanged until another increase at Day 5. At 2$0^{\circ}C$, the pH increased rather rapidly at early stage of fermentation and reached 8.9 at Day 4. The pattern of $NH_4^+$ concentration of fermented skate extract was similar to that of pH. Particularly at $4^{\circ}C$ fermentation, $NH_4^+$ concentration was not affected by fermentation time. The concentration of $NH_4^+$. reached approximately $10.2\mug/mL$at $10^{\circ}C$ for Day 5 and $20^{\circ}C$ for Day 4-5, indicating the early stage of fermentation. According to physiological activities of hot water extracts of skate fillet and viscera as affected by fermentation time, antibacterial activity of 2% viscera extract concentration was 43.3% at Day 8, while there was no antibacterial activity from fillet extract. As for the antioxidative activity, fillet extract and viscera extract both at 2% concentration at Day 0 showed 61.2% and 54.4%, respectively. Anticancer activities were highest (52.7% for fillet extract and 58.3% for viscera extract) at $1,000 \mug/mL$ concentration at Day 8. Antibacterial activities and anticancer effects were relatively high as fermentation was progressed, while antioxidative activities were highest before fermentation started. As for the physiological activities of hot water extract from brain and cartilage, antibacterial activities were observed at 41.0% when 2% brain extract was added at 4 hours of incubation, while 35.8% with 2% cartilage extract at 14 hours of incubation. Antioxidative activities were not found in brain extract, but cartilage extract at 2% showed 25.0% of antioxidative activity, which was lower than fillet and viscera extract.

Preparation and Photoluminescence of Mn-Doped $ZnGa_2O_4$ Phosphors (Mn 도핑한 $ZnGa_2O_4$ 형광체의 제조 및 빛발광 특성)

  • 류호진;박희동
    • Journal of the Korean Ceramic Society
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    • v.33 no.5
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    • pp.531-535
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    • 1996
  • ZnGa2O4 and Mn-doped ZnGa2O4 were synthesized using the state reaction method to investigate their photoluminescence characteristics depending on Mn concentration. Under 254nm excitation, ZnGa2O4 exhibited a broad-band emission extending from 330 nm to 610 nm peaking at 450nm. On the other hand Mn-doped ZnGa2O4 showed a new strong narrow-band emission peaking at 504 nm and maximum intensity at the doping concentration of 0.006 mole Mn.

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Chemistry and Crystallographic Studies of Metal Ion Exchanged Zeolite X. Ⅰ. The Crystal Structure of Fully Dehydrated and Fully $K^+$-Exchanged Zeolite X, $K_{92}$-X

  • 장세복;김양
    • Bulletin of the Korean Chemical Society
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    • v.16 no.6
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    • pp.539-542
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    • 1995
  • The crystal structure of K92-X (K92Al92Si100O384), a=25.128(1) Å, dehydrated at 360 ℃ and 2X 10-6 Torr, has been determined by single-crystal X-ray diffraction techniques in the cubic space group Fd&bar{3} at 21(1) ℃. The structure was refined to the final error indices R1=0.044 and Rw=0.039 with 242 reflections for which I<3σ(I). In this structure, ninety-two K+ ions are located at the five different crystallographic sites. Sixteen K+ ions are located at the centers of the double six rings (site I; K(1)-O(3)=2.65(2) Å and O(3)-K(1)-O(3)=92.0(6)°). About twelve K+ ions lie at site I' in the sodalite cavity opposite double six rings (D6R's) and these K+ ions are recessed ca. 1.62 Å into the sodalite cavity from their O(3) plane (K(2)-O(3)=2.74(2) Å, O(3)-K(2)-O(3)=88.5(8)°). About thirty-two K+ ions are located at the site II in the supercage and these K+ ions are recessed ca. 1.20 Å into the supercage from their O(2) plane (K(3)-O(2)=2.64(2) Å, and O(2)-K(3)-O(2)=101(1)°). About twenty-two K+ ions lie at the site III in the supercage opposite 4-ring ladder and the remaining ten K+ ions lie at the site III' near the 4-ring ladder in the supercage (K(4)-O(4)=2.88(3) Å, O(4)-K(4)-O(4)=79.8(9)°, K(5)-O(4)=2.8(2) Å, and O(4)-K(5)-O(4)=68(5)°).

Qualitative Changes in Precooling and Storage Temperatures on the Post-harvest Management of the Fruits in Strawberry for Export (수출딸기의 수확 후 예냉 및 저장온도에 따른 품질변화)

  • Park, Ji-Eun;Kim, Hye-Min;Hwang, Seung-Jae
    • Journal of agriculture & life science
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    • v.46 no.6
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    • pp.67-74
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    • 2012
  • A study was conducted to examine the effect of precooling and storage temperatures on the storage life of 'Maehyang' strawberry for export. Fruits at a 80% ripe stage were harvested from a commercial greenhouse in Gyeongsangnamdo Jinju on January 11, 2011. Fruits were precooled at 0, 2, $4^{\circ}C$ for 2-5 hours, and then transported for about 30 minutes. And stored in set chamber at 4, 8, $10^{\circ}C$, immediately. Fruits were examined for their changes in weight, hardness, Hunter color values, soluble solid content (SSC), acidity, and incidence of gray mold (Botrytis cinerea) during storage. The fresh weight decreased during storage at all temperatures. Especially, strawberry treated the lowest precooling and storage temperatures was the greatest in quality. The hardness was drastically decreased in precooled at $4^{\circ}C$ and stored at $10^{\circ}C$ after storage 10 days. SSC was the greatest when strawberry stored at the lowest temperature. Acidity increased during storage at all temperatures. But in the last day after storage, the strawberry of precooled at $4^{\circ}C$ and stored at $10^{\circ}C$ was the highest as 0.7%. Gray mold was the greatest at $10^{\circ}C$ storage temperature. The results indicate that effectiveness for keeping the freshness was best achieved by precooling at $0^{\circ}C$ and storage at $4^{\circ}C$.

Growth and Yield Responses of Soybean to Overhead Flooding Duration at Four Growth Stages (관수시간에 따른 콩의 생육 및 수량반응)

  • 박경열;이종형;조영철
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.1
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    • pp.92-97
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    • 1995
  • The objective of this study is to investigate the growth characters of overhead flooded soybean plants at four growth stage. Overhead flooding treatments were applied at the vegetative growth stage ($V_3,\;V_6$) and the reproductive stage ($R_2,\;R_4$) for 6.12.24 hrs, respectively. Yield and yield components were more decreased as the overhead flooding duration was longer and the growth stage was later. Yield was not reduced significantly in soybean plants flooded at $V_3$ stage regardless of flooding duration, and flooded 6 or 12 hrs at $V_6$ stage. When compared to the control, 27 to 36% of yield reduction was observed in soybean plants flooded for 24 hrs at $V_6$ stage, 6 or 12 hrs at $R_2$ stage, and 6 hrs at $R_4$ stage. And 43%, 53% and 66% of yield were reduced through the flooding treatment for 24 hrs at $R_2$ stage 12 hrs and 24 hrs at $R_4$ stage, respectively. So yield reduction was higher in overhead flooded soybean plants at the reproductive stage than that at the vegetative growth stage.

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A Study on the Sleep Activity Pattern of the Infant (대구 지역 영아의 수면/활동 양상에 관한 연구)

  • Kim, Mi-Ye;Koh, Hyo-Jung;Shin, Young-Hee;Kim, Young-Hee;Oh, Jin-A;Kim, Hye-Young;Lee, Eun-Ju;Kim, Yong-Sook
    • Korean Parent-Child Health Journal
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    • v.3 no.2
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    • pp.1-17
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    • 2000
  • The purpose of this study was to recognized the sleep activity patterns of the normal infants in Korea. The subject were 41 normal infants who were below 8 weeks old and were born in Taegu city and Kyungbuk province. The measuring instrument the sleep activity was NCASA translated by the Korean-parent-child Health Academic Association. The data that were collected from April, 18 1999 to January 1, 2000 were analysed by the SPSS 7.5 program. The results of the study was summarized as follow; 1) The mean amount of total sleeping hours per day was decreased from $16.27{\pm}1.96$ hr at the 1st week, to $15.19{\pm}2.39$ hr at the 2nd week, to $13.66{\pm}2.40$ hr at the 3 week but increased to $16.55{\pm}5.77$ hr at the 4-8 week after birth. 2) The mean frequency of feeding was increased to $9.43{\pm}2.79$ times at the 3rd week but decreased to $7.55{\pm}1.23$ times at the 4-8th week after birth. 3) The regularity of day time sleep was decreased from 50.00% to 19.79% and that of night time sleep was increased from 51.79% to 72.92%. 4) The regularity of feeding was increased from 30.42% at the 1st week to 50.07% at the 2nd week but decreased 26.34% at the 4-8th week after birth. 5) The longest period of day time sleep was decreased to $2.39{\pm}0.42$ hr at the 3rd week but increased $2.47{\pm}0.69$ hr at the 4-8th week after birth. The longest period of night time sleep was decreased to $2.58{\pm}0.67$ hr at the 2nd week but increased to $3.00{\pm}1.32$ at the 3rd week and $4.30{\pm}1.16$ hr at the 4-8th week after birth. 6) At the 1st week, the 2nd week, the 3rd week after birth, the frequency of day time feeding was positively correlated with the frequency of night time feeding($R_{1week}=0.948^{**}$, $R_{2week}=0.927^{**}$, $R_{3week}=0.779^{**})$. At the 4-8th week after birth, the frequency of night time feeding was negatively correlated with the amount of night time sleep ($R_{4week-8week}=0.875^{*}$). On the basis of above findings, the following is suggested; It is need a longitudinal and transcultural study which are designed for children's sleep and feeding.

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A Study on Properties of Low Temperature Sintering in the NiZn Ferrite System (NiZn 페라이트의 저온 소결 특성에 관한 연구)

  • 고상기;김병호;김경용
    • Journal of the Korean Ceramic Society
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    • v.34 no.10
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    • pp.1074-1082
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    • 1997
  • Microstructure and permeability as a function of sintering temperature and composition were studied on the Ni$\delta$Cu0.4-$\delta$Zn0.6Fe2O4 ($\delta$=0, 0.1, 0.2, 0.3, 0.4) which was prepared by Cu2+ substitution for Ni2+ in Ni.0.4Zn0.6Fe2O4, then followed by 8 wt% CuO and 1wt% Bi2O3 as sintering aids. It was found that NiCuZn ferrite in which Cu2+ is substituted for Ni2+ is more effective in reduction of sintering temperature than Ni.0.4Zn0.6Fe2O4, containing CuO as a sintering aid. The specimen $\delta$=0.2 sintered at 90$0^{\circ}C$ for 2hr exhibited the highest initial permeability value ($\mu$o=280 at 1Mhz), but the real permeability decreased at the frequency under 10 MHz. EPMA analysis showed that Ni$\delta$Cu0.4-$\delta$Zn0.6Fe2O4 ($\delta$=0.4), sintered at 95$0^{\circ}C$ for 2hrs consisted of three phase regions of Ni.0.3Cu0.1Zn0.6Fe2O4 region, Cu and Bi liquid existed at the 3-point boundary, although the stabilization energy of Ni2+ is higher than that of Cu2+ in B site.

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Cardioprotective Efficacy of Ischemic Preconditioning on Long-Term Myocardial Preservation in Isolated Rat Heart (적출 쥐 심장에서 장시간의 심장보존시 허혈성 전조건화가 심근보호에 미치는 영향)

  • 허동명;장봉현
    • Journal of Chest Surgery
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    • v.33 no.8
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    • pp.605-612
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    • 2000
  • Background: Ischemic preconditioning enhances the tolerance of myocardium against ischemia/reperfusion injury, with the enhancement of the recovery of post-ischemic myocardial function. This study was disigned to assess whether the protective effect of ischemic preconditioning could provide one additional hour of myocardial preservation in four hour myocardial ischemia in a rate heart. Material and method: Fourty four Spargue-Dawley rats, weighing 300~450gm, were divided into four groups. Group 1(n=7) and group 3(n=12) were subjected to 30 minutes of aerobic Langendorff perfusion without ischemic preconditioning and then preserved in saline solution at 2~4$^{\circ}C$ for 4 hours and 5 respectively. Group 2(n=7) and group 4(n=18) were perfused in the same way for 20 minutes, followed by 3 minutes of global mormothermic ischemia and 10 minutes of perfusion and then preserved in the same cold saline solution for 4 hours and 5 hours respectively. Heart rate, left ventricular developed pressure(LVDP), and coronary flow were measured at 15 minutes during perfusion as baseline. Spontaneous defibrillation time was measured after reperfusion. Heart rate, LVDP, and coronary flow were also recorded at 15 minutes, 30 minutes, and 45 minutes during reperfusion. Samples of the apical left ventricular wall were studied using a transmission electron microscope. Result: Time of spontaneous defibrillation(TSD) was significantly longer in group 4 than in group 1(p<0.001), and TSD in group 1 was significantly longer in comparision to that of group 2(p<0.05). Heart rate at 45 minutes was significantly higher in group 1 than in group 4(p<0.05). Heart rate at 15 min was significantly higher in group 2 than in group 1(p<0.001) and in group 4 than in group 3(p<0.05). Left ventricular developed pressure(LVDP) at 30 minutes and 45 minutes was higher in group 1 than in group 4(p<0.01), LVDP at 45 minutes was higher in group 4 than in group 3(p<0.05). Rate-pressure product(RPP) at 30 minutes and 45 minutes was higher in group 1 than in group 4(p<0.05). RPP at 15 minutes was higher in group 2 than in group 1(p<0.01). RPP at 30 minutes and 45 minutes was higher in group 4 than in group 3(p<0.05). Group 2 showed relatively less sarcoplasmic edema and less nuclear chromatin clearance than group 1. Group 4 showed less myocardial cell damage than group 3, group 4 showed less myocardial cell damage than group 3, group 4 showed more myocardial cell edema than group 1. Conclusion: Ischemic preconditioning enhanced the recovery of postischemic myocardial function after 4 hours and 5 hours preservation. However, it was not demonstrated that ischemic preconditioning could definitely provide one additional hour of myocardial preservation in four hour myocardial ischemia in a rat heart.

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