• 제목/요약/키워드: $AB^2+C$

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Rapid Dot-Blot Immunoassay for Detecting Multiple Salmonella enterica Serotypes

  • Jeongik Cho;Heymin Song;Hyun C. Yoon;Hyunjin Yoon
    • Journal of Microbiology and Biotechnology
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    • 제34권2호
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    • pp.340-348
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    • 2024
  • Salmonella, a major contributor to foodborne infections, typically causes self-limiting gastroenteritis. However, it is frequently invasive and disseminates across the intestinal epithelium, leading to deadly bacteremia. Although the genus is subdivided into >2,600 serotypes based on their antigenic determinants, only few serotypes are responsible for most human infections. In this study, a rapid dot-blot immunoassay was developed to diagnose multiple Salmonella enterica serotypes with high incidence rates in humans. The feasibility of 10 commercial antibodies (four polyclonal and six monoclonal antibodies) was tested using the 18 serotypes associated with 67.5% Salmonella infection cases in the United States of America (U.S.A) in 2016. Ab 3 (polyclonal; eight of 18 serotypes), Ab 8 (monoclonal; 13 of 18 serotypes), and Ab 9 (monoclonal; 10 of 18 serotypes) antibodies exhibited high detection rates in western blotting and combinations of two antibodies (Ab 3+8, Ab 3+9, and Ab 8+9) were applied to dot-blot assays. The combination of Ab 3+8 identified 15 of the tested 18 serotypes in 3 h, i.e., S. Enteritidis, S. Typhimurium, S. Javiana, S. I 4,[5],12:i:-, S. Infantis, S. Montevideo, S. Braenderup, S. Thompson, S. Saintpaul, S. Heidelberg, S. Oranienburg, S. Bareilly, S. Berta, S. Agona, and S. Anatum, which were responsible for 53.7% Salmonella infections in the U.S. in 2016. This cost-effective and rapid method can be utilized as an on-site colorimetric method for Salmonella detection.

Marked Expansion of CD11c+CD8+ T-Cells in Melanoma-bearing Mice Induced by Anti-4-1BB Monoclonal Antibody

  • Ju, Seong-A;Park, Sang-Min;Lee, Sang-Chul;Kwon, Byoung S.;Kim, Byung-Sam
    • Molecules and Cells
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    • 제24권1호
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    • pp.132-138
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    • 2007
  • 4-1BB (CD137), a member of the tumor necrosis factor receptor superfamily, is expressed on activated T-cells, and 4-1BB signaling due to interaction with 4-1BB ligand or ligation with anti-4-1BB monoclonal antibody (mAb) costimulates T cells. It has been shown that administration of anti-4-1BB mAb induces anti-tumor immunity in mice, but the nature of the cellular subsets responsible for this immunity is uncertain. In this study we found that anti-4-1BB mAb administration to B16F10 melanoma-bearing mice induced marked expansion of $CD11c^+CD8^+$ T-cells in parallel with suppression of pulmonary tumors. The mAb-treated mice produced higher levels of $IFN-{\gamma}$ in their tumor tissues, spleen and lymph nodes than mice exposed to control antibody. When the $CD11c^+CD8^+$ T-cells were purified and re-stimulated in vitro, they produced high levels of the Th1 cytokines, $IFN-{\gamma}$ and IL-2, but low levels of the Th2 cytokines, IL-4 and IL-10. Furthermore, they expressed high levels of 4-1BB and CD107a, a marker of activated cytotoxic T-lymphocytes. Our results suggest that $CD11c^+CD8^+$ T-cells play a role in the anti-tumor immunity induced by anti-4-1BB mAb.

버어리주맥과 엽설의 니코틴과 nitrate함량에 의한 등급별 군집분석 (The Grades Classification of Burley Stems and Scraps using Cluster Analysis by Nicotine and Nitrate Contents)

  • 한영림;성용주;권영주;김삼곤;이경구;김근수;이문수
    • 한국연초학회지
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    • 제28권2호
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    • pp.124-129
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    • 2006
  • The grades of burley tobacco stems and scraps were classified followed by the nicotine and nitrate contents by using the cluster analysis. The chemical components of the burley tobacco stems and scraps gathered from 2002 to 2005 were analyzed with auto analyzer. According to the nicotine contents and the nitrate contents, the burley stems and scraps could be classified three groups, respectively. In case of the burley scraps, the AB3T, AB4TR, B1T and B2T grades belonged to the $1^{st}$ a group. The C1W and C2W grades belonged to the $2^{nd}$ group and the CD3W and CD4TR belonged to the $3^{rd}$. In case of the burley stems, the AB3T and AB4TR grades belonged to the $1^{st}$ group. The B1T, B2T, C1W and C2W grades belonged to the $2^{nd}$ group and the CD3W and CD4TR belonged to the $3^{rd}$ group. This classification of raw materials depending on the similarity in the chemical components might be helpful to control the properties of the Reconstituted Tobacco sheet.

A MEAN VALUE FUNCTION AND ITS COMPUTATIONAL FORMULA RELATED TO D. H. LEHMER'S PROBLEM

  • Wang, Tingting
    • 대한수학회보
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    • 제53권2호
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    • pp.487-494
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    • 2016
  • Let p be an odd prime and c be a fixed integer with (c, p) = 1. For each integer a with $1{\leq}a{\leq}p-1$, it is clear that there exists one and only one b with $0{\leq}b{\leq}p-1$ such that $ab{\equiv}c$ mod p. Let N(c, p) denote the number of all solutions of the congruence equation $ab{\equiv}c$ mod p for $1{\leq}a$, $b{{\leq}}p-1$ in which a and $\bar{b}$ are of opposite parity, where $\bar{b}$ is defined by the congruence equation $b{\bar{b}}{\equiv}1$ mod p. The main purpose of this paper is using the mean value theorem of Dirichlet L-functions and the properties of Gauss sums to study the computational problem of one kind mean value function related to $E(c,p)=N(c,p)-{\frac{1}{2}}{\phi}(p)$, and give its an exact computational formula.

CD4O Activation Protects Dendritic Cells from Anticancer Drug-Induced Apoptosis

  • Jun, Jae-Yeon;Joo, Hong-Gu
    • The Korean Journal of Physiology and Pharmacology
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    • 제7권5호
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    • pp.255-259
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    • 2003
  • Dendritic cells (DCs) play a critical role in various immune responses involving $CD4^+$ T cells and have been used to generate anti-tumor immunity. Chemotherapy induces severe side effects including immunosuppression in patients with cancer. Although immunosuppression has been studied, the effects of anticancer drugs on DCs are not fully determined. In this study, we demonstrated that CD40 activation strongly protected DCs from 5-fluorouracil (5-FU) or mitomycin C-induced apoptosis. DCspecific surface markers, including CD11c and major histocompatibility complex (MHC) class II, were used for identifying DCs. CD 40 activation with anti-CD40 mAb significantly enhanced the viability of DCs treated with 5-FU or mitomycin C, assayed by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide). Fluorescence staining and analysis clearly confirmed the enhancing effect of anti-CD40 mAb on the viability of DCs, suggesting that CD40 activation may transduce critical signals for the viability of DCs. Annexin V staining assay showed that CD40 significantly protected DCs from 5-FU or mitomycin C-induced apoptosis. Taken together, this study shows that CD40 activation with anti-CD40 mAb has strong anti-apoptosis effect on DCs, suggesting that CD40 activation may overcome the immunosuppression, especially downregulation of number and function of DCs in chemotherapy-treated cancer patients.

남해 연안에 생육하는 거머리말(Zostera marina L.) 개체군의 생물계절학과 생식 능력 (Phenology and Reproductive Effort of Two Zostera marina L. Populations on the Southern Coast of Korea)

  • 이상룡;이성미;최청일
    • Ocean and Polar Research
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    • 제27권1호
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    • pp.67-74
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    • 2005
  • 남해안의 동대만과 앵강만에 생육하는 거머리말 개체군의 생물계절학과 생식 능력을 2002년 9월부터 2003년 8월까지 조사하였다. 동대만의 퇴적물 입도는 앵강만보다 세립하였으며, 퇴적물의 유기물 함량도 유의하게 높았다. 동대만의 수온은 $7.5^{\circ}C$(2월)에서 $25.5^{\circ}C$(8월)까지 분포하였으며, 앵강만은$6.7^{\circ}C$(2월)에서부터 $25.2^{\circ}C$(9월)까지 분포하였다. 두 지역의 거머리말 개체군은 다년생의 생활사를 보였으며, 생식지의 생식 능력들은 동대만이 앵강만보다 유의하게 높았다. 동대만의 생식지는 2003년 2월부터 6월까지 출현하였으나, 앵강만에서는 2003년 3월부터 6월까지 출현하였다. 동대만에서 생식지의 생육밀도는 $11{\sim}33%$를 차지하였으나, 동대만에서는 5% 이하로 나타났다. 생식지의 구조적인 특징은 육수화서 내 열매와 종자 수를 제외하고 동대만에서 유의하게 높았다. 두 생육지에서 거머리말 개체군의 생식 단계의 출현 시기는 수온과 관계하는 것으로 사료되었다.

고콜레스테롤 식이의 급여에 의해 고지혈증이 유도된 흰쥐의 신령버섯 자실체의 항고지혈증 효과 (Anti-hyperlipidemic effect of Agaricus brasiliensis fruiting bodies in rats fed with high cholesterol diet)

  • 진가헌;이태수
    • 한국버섯학회지
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    • 제16권2호
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    • pp.118-124
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    • 2018
  • 신령버섯의 자실체가 고콜레스테롤을 급여한 Sprague Dawley계 암컷 흰쥐의 지질대사와 생리활성에 미치는 영향을 조사하기 위해 생후 5주령의 흰쥐에 표준식이를 급여한 정상 식이군(NC), 정상 식이에 10%의 대두유와 1%의 콜레스테롤을 첨가하여 급여한 고콜레스테롤 식이군(HC), 고콜레스테롤 식이에 신령버섯 자실체 분말을 5% 첨가하여 급여한 군(HC+AB) 등 3개의 실험군으로 나누어 6주간 실험을 수행하였다. 실험동물의 체중증가와 식이효율은 HC에 비해 HC+AB가 유의하게 감소하여 NC와 유사한 수준을 나타냈다. 식이섭취량은 NC에 비해 HC와 HC+AB가 유의하게 낮았다. 혈청의 총콜레스테롤(TC), LDL-콜레스테롤(LDL-C), 중성지방(TG)의 농도는 HC에 비해 NC와 HC+AB가 유의하게 낮았으며 심혈관계 질환의 위험도를 나타내는 동맥경화지수(AI)와 LDL-C/HDL-C의 비율도 HC에 비해 NC와 HC+AB가 유의하게 낮았다. 혈청의 알부민, 크레아티닌, 요산 및 총단백질의 농도도 NC와 HC+AB가 HC에 비해 조금 낮았다. 간기능의 척도를 나타내는 혈청의 GOT와 GPT 효소의 활성은 NC와 HC+AB가 HC에 비해 낮았지만 유의성을 없었고, ALP의 농도는 HC에 비해 NC와 HC+AB가 유의하게 낮아서 신령버섯 자실체 분말의 급여가 흰쥐의 ALP 농도를 낮추는 효과가 있었다. 실험 5-6주 사이에 배출된 변을 채취하여 분석한 총지질과 총콜레스테롤의 양은 HC+AB가 NC와 HC에 비해 유의하게 높아 식이로 섭취한 일부의 지방과 콜레스테롤이 장에서 흡수되지 않고 변으로 배출되는 것으로 나타났다. 따라서 고콜레스테롤 식이에 신령버섯의 자실체 분말을 5% 수준으로 첨가하여 6주간 급여한 흰쥐의 체중, 혈청의 TC, LDL-C 및 TG의 농도와 동맥경화 수치(AI)가 정상 수준을 유지하고 지방간의 발생도 억제되는 효과가 현저히 나타나 고지방과 고콜레스테롤의 섭취에 의해 발생할 수 있는 심혈관계 질환의 예방과 개선에 신령버섯의 자실체가 효과를 나타내는 것으로 사료된다.

AB급 CMOS 전류 콘베이어(CCII)에 관한 연구 (A study of class AB CMOS current conveyors)

  • 차형우;김종필
    • 전자공학회논문지C
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    • 제34C권10호
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    • pp.19-26
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    • 1997
  • Novel class AB CMOS second-generation current conveyors (CCII) using 0.6.mu.m n-well CMOS process for high-frequency current-mode signal processing were developed. The CCII for low power operation consists of a class AB push-pull stage for the current input, a complementary source follower for the voltage input, and a cascode current mirror for the current output. In this architecture, the two input stages are coupled by current mirrors to reduce the current input impedance. Measurements of the fabricated CCII show that the current input impedance is 875.ohm. and the bandwidth of flat gain when used as a voltage amplifier extends beyond 4MHz. The power dissipation is 1.25mW and the active chip area is 0.2*0.15[mm$\^$2/].

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산란계종의 잡잡강세리용에 관한 연구 (Study on Utilization of Heterosis in Layer Chicken)

  • 오봉국;여정수;이정구;이문연
    • 한국가금학회지
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    • 제7권2호
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    • pp.28-36
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    • 1980
  • This study was carried out to estimate combining abilitie or economic traits in layer chickens. The data used in this study were the record of 10 single crosses produced by half diallel cross of 5 lines of Single Comb White Leghorns, such as A, B, C, K and S lines. Total 720 progenies of the crosses were reared at the Poultry Breeding Farm, College of Agriculture, Seoul National University from Feb. 1979 to August, 1980. Combining abilities were estimated by Grilling's mathematical model for the traits; age the first egg, total egg number, egg weight and body weight. The results attained from the studies were summarized as follows; In estimate of combining ability, an age at first egg of BS cross was largely due to significiantly higher general combining ability (G. C. A.) effect of B and S strains than Cand K strains in G. C. A. effect, and to specific combining ability (S. C. A) effect of B and S-trains. AB and BS crosses showed the highest egg Production. AB cross performance was result from high G.C.A. effect of A ana B strains. BS cross performance was result from high G. C. A. effect of B and high S. C. A. effect of BS cross. Specific combining ability effect in egg. weight was not statiscally significiant, but S strain showed high G. C. A. effect. A and B strains in body weight showed significantly low C. C. A. effect. From the above results, BS cross in an age at first egg, AB and BS crosses in egg Production, S strain in egg weight and AB cross in body weight were superior to other strains or crosses.

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Lactobacillus plantarum 첨가 고추장의 C57BL/6 마우스에서 대장염 예방 증진효과 (Improvement of colitis preventive effects of Gochujang by addition of Lactobacillus plantarum on C57BL/6 mice)

  • 박의성;허주희;임양이;주재현;박건영
    • 한국식품저장유통학회지
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    • 제24권8호
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    • pp.1188-1194
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    • 2017
  • DSS로 대장염을 유도한 C57BL/6 마우스에게 잡곡(보리, 수수, 기장)과 죽염, 종균(AB, ABL)을 접종한 메주로 만든 고추장을 섭취시켰다. MG-AB와 MG-ABL을 섭취한 마우스는 control에 비교해 대장의 길이를 증가시키고, 무게/길이비율은 감소시켜 대장염의 염증반응을 억제시켰다. MG-ABL은 혈청에서 IL-6의 발현을 control과 MG-AB군에 비해 유의적으로 감소시켰고, apoptosis 관련 인자인 Bax와 Bcl-2의 발현을 조절하여 apoptosis를 유도하였다. 따라서 MG-ABL은 발효 시 첨가된 Lab. plantarum로 인해 대장조직의 손상을 억제하고 전염증성 사이토카인과 apoptosis 관련 인자를 조절하여 대장염을 억제하는 것으로 나타났다.