• 한국작물학회지
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• 제40권2호
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• pp.250-254
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• 1995

분리한 보리호분층을 이용하여 $(1-3)-{\beta}-glu-canase$와 $(1-3,1-4)-{\beta}-glucanase$의${\;}GA_3$에 대한 반응특성을 조사하였다 $GA_3$ 처리에 의해 호분충과 배양액 내의 단백질 함량, $(1-3)-{\beta}-glucanase{\;}$및${\;}(1-3,1-4)-{\beta}-glucanase$ 활성 등이 모두 증가하였다. 그러나 $GA_3$ 처리에 의한 효소활성 및 효소분비 증가 정도는 효소에 따라 큰 차이를 보였다. $(1-3,1-4)-{\beta}-glucanase$가${\;}(1-3)-{\beta}-glucanase$보다 효소활성 증가 정도와 분비증가 정도 모두 컸다. 이와 같은 결과는 발아초기 $(1-3,1-4)-{\beta}-glucanase$의 중요한 생리작용과 관계가 있을 것으로 생각되었다.

• Natural Product Sciences
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• 제2권1호
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• pp.19-23
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• 1996

A mixture of sterols containing ${\beta}-sitosterol$ and stigmasterol (1), and sterol glycosides containing $3-O-{\beta}-D-glucopyranosyl$ ${\beta}-sitosterol$ and $3-O-{\beta}-D-glucopyranosyl$ stigmasterol (2) were isolated from the leaves of Gynura procumbens. After acetylation of 2 with pyridine-acetic anhydride, $3-0-{\beta}-D-tetra-O-acetylglucopyranosyl$ ${\beta}>-sitosterol$ (3) was isolated.

• 대한약리학회지
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• 제27권2호
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• pp.119-123
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• 1991

${\beta}-$수용체 효능약물 ((-)-NE), 길항약물 $((\pm)-propranolol,\;labetalol)$ 및 PDE 억제약물(imazodan, KR-30045, KR-30075 등)에 대한 ${\beta}-adrenoceptor$ binding 실험을 ${\beta}_1/{\beta}_2$ 비선택적 radioligand인 $(-)-[^3H]-DHA$를 사용하여 실시하였다. Saturation 실험에서 ${\beta}_1$ 및 ${\beta}_2$ 수용체를 모두 갖고 있는 rat 좌심실의 ${\beta}$ 수용체에 대한 $(-)-[^3H]-DHA$의 $K_d$ 값은 $1.5{\pm}0.43\;nM$, $B_{max}$는 $22.0{\pm}0.9\;fmol/mg$ protein이었다. $({\pm})propranolol$, labetalol 및 (-)NE는 단일상으로 $(-)-[^3H]-DHA$의 결합을 억제하였으며 Ki 값은 각각 $17.0{\pm}0.43\;nM$, $57.3{\pm}1.30\;nM$, $1.57{\pm}0.95\;{\mu}M$로 나타났다. 실험에 사용한 모든 PDE 억제약물들은 $(-)-[^3H]-DHA$ 결합을 $10^{-3}\;M$의 고농도에서도 10% 미만으로 억제했다. 실험결과, propraolol, labetalol 및 NE는 ${\beta}_1/{\beta}_2$ 수용체에 대해 비선택적인 약물로 나타났으며, imazodan 및 신합성 PDE 억제약물들은 rat 심근에 있는 ${\beta}-$수용체에 친화성이 거의 없음을 알 수 있었다.

• 한국식품과학회지
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• 제28권3호
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• pp.411-416
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• 1996

초임계 유체를 이용하여 당근(Daucus carrota L.) 중의 ${\beta}-carotene$ (Y)을 추출하는 최적조건을 규명하기 위하여 추출압력(X_1$ 200-300bar), 온도($X_2,\;35-51^{\circ}C$) 및 시간($X_3$ 60-200min)을 선정하고 중심합성에 의한 실험계획을 설정하였다. 선정된 독립인자($X_1,\;X_2,\;X_3$)의 반응표면에 대한 영향을 분석하고 2차 다항 회귀모형식을$Y={\beta}_0+{\beta}_1X_1+{\beta}_2X_2+{\beta}_3X_3+{\beta}_11X_12+{\beta}_22X_3^2+{\beta}_-12X_1X_2+{\beta}_12X_1X_2+{\beta}_13X_1X_3+{\beta}_23X_2X_3$로 하여 linear, quadratic 및 interaction effects를 관찰하여 다음과 같은 결과를 얻었다. 1) ${\beta}-carotene$ 추출의 주요 인자는 압력, 시간, 온도의 순이었으며 이중, 중심점과 압력인자의 선형회귀 효과는 ${\alpha}(2)$가 0.001(99.9%) 수준에서 유의하였으며 그 회귀 계수는 다음과 같다. ${\beta}(0)\;5.83{\times}10^6,\;{\beta}(1)3.67{\times}10^6,\;{\beta}(2)\;1.24{\times}10^6,\;{\beta}(3)1.01{\times}10^6,\;{\beta}(11)\;-1.23{\times}10^6,\;{\beta}(22)-8.19{\times}10^5\;{\beta}(33)\;-9.79{\times}10^5,\;{\beta}(12)3.31{\times}10^5\;{\beta}(33)\;-9.79{\times}10^5,\;{\beta}(12)3.31{\times}10^5\;{\beta}(13)\;5.18{\times}10^5,\;{\beta}(23)9.08{\times}10^5$ 2) 회귀식에 대한 분산분석 결과, 분산비(Fo)가 8.44로 0.05 수준에서 모델에 의해 도출된 결과를 잘 성명할 수 있으며 그 정확도에 대한 결정계수($r^2$)는 0.938로 높았다. 또한 정상점에서 ${\beta}-carotene$의 반응표면을 정준분석한 결과 중속 변량인 ${\beta}-carotene$추출함량이 최대점임을 확인하였다. 3) 최적조건 즉, 압력은 350bar, 온도는 $51^{\circ}C$ 및 시간은 200min의 조건을 동시에 만족하는 관심영역에서의 ${\beta}-carotene$의 최대추출량은 생당근 100g당 10,611 ${\mu}g$으로 예측되었다.

• 약학회지
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• 제43권1호
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• pp.5-10
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• 1999

As one of the serial studies on the specific and indigenous plants of Mt. Chiri the constituents of aerial part from filipendula formosa (Rosaceae) were investigated. From of the MeOH extract, five flavonol glycosides, kaempferol-3-O-$\beta$-D-galactopyranoside, querecetin-3-O-$\beta$-D-galactopyranoside, quercetin-3-O-$\alpha$-Lrhamopyranosyl (1 6)-$\beta$-D-galactopyranoside, kaempferol-3-O-$\alpha$-L-rhamnopyranosyl (1 6)-$\beta$-D-galactopyranoside and quercetin-7-O-$\beta$-D-glucopyranosy-3-O-$\beta$-D-galactopyranoside were isolated by column chromatographic separation using Amberlite XAD-2 and Sephadex LH-20, and identified physicochemical evidences (IR, FAB-Mass, $^1H,{\;}^{13}C-NMR$).

• 약학회지
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• 제44권4호
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• pp.334-339
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• 2000

The present study was carried out to evaluate biologically active components of the rhizomes of Sparganium stoloniferum and to supply the preliminary data for the chemotaxonomy and the medicinal application. Extraction and systematic fractionation of the rhizomes by column chromatography led to the isolation of six compounds from ethylacetate and n-butanol soluble fractions. Elucidation of the chemical structures of these compounds by physicochemical and apectral analysis demonstrated that compound I,II ,III,IV,V and Ⅵ were $\beta$-sitosterol, $\beta$-sitosterol-3-$\beta$-D-glucuronopyranoside, 3- (4-hydroxyphenyl)-2-propenoic acid, sorbose, 1-O-$\beta$-D-glucopyranosyl-(2S, 3R, 4E, 8Z)-2-[(2(R)-hydroxyeicosanoyl)amido]-4,8-octadecadiene-1,3-diol, and $\beta$-sitosterol-3-O-$\beta$-D-glucopyranoside, respectively.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2003년도 추계학술발표대회 논문요약집
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• pp.68-69
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• 2003

The present study was conducted to investigate the effects of dietary supplementation of $\beta$-1,3 glucan and feed stimulants(BAISM) as a feed additive for juvenile olive flounder (Paralichthys olivaceus). Eight experimental diets supplemented with $\beta$-1,3 glucan and feed stimulants at 0%, $\beta$-1,3 glucan 0.05% ＋ Baism 0.45%, $\beta$-1,3 glucan 0.05% ＋ Baism 0.95%, $\beta$-1,3 glucan 0.10% ＋ Baism 0.90%, $\beta$-1,3 glucan 0.10% ＋ Baism 1.90%, $\beta$-1,3 glucan 0.15% ＋ Baism 1.35%, $\beta$-1,3 glucan 0.15% ＋ Baism 2.85% and $\beta$-1,3 glucan 0.30% ＋ Baism 2.70% of diets as a dry-matter(DM) basis were prepared. Three replicate groups of fish averaging 9.2 $\pm$ 0.1g (Mean $\pm$ SD) were randomly distributed in each aquarium as a group of 15 fish and fed one of eight experimental diets for seven weeks. After the feeding trial, $\beta$-1,3 glucan 0.10% ＋ Baism 0.90%, $\beta$-1,3 glucan 0.10% ＋ Baism 1.90% diets had a higher weight gain (WG), feed efficiency(FE), specific growth rate(SGR) and protein efficiency ratio(PER) than did fish fed 0%, $\beta$-1,3 glucan 0.05% ＋ Baism 0.45%, $\beta$-1,3 glucan 0.05% ＋ Baism 0.95%, $\beta$-1,3 glucan 0.15% ＋ Baism 2.85% and $\beta$-1,3 glucan 0.30% ＋ Baism 2.70% (P<0.05). however, there was no significant difference among fish fed $\beta$-1,3 glucan 0.05% ＋ Baism 0.45%, $\beta$-1,3 glucan 0.05% ＋ Baism 0.95%, $\beta$-1,3 glucan 0.15% ＋ Baism 2.85% and $\beta$-1,3 glucan 0.30% ＋ Baism 2.70%(P>0.05). and $\beta$-1,3 glucan 0.10% ＋ Baism 0.90% diets had a higher peak value of CL(Chemiluminescence) and lysozyme activity, than did fish fed the other diets (P<0.05). These results indicated that dietary sipplementation of $\beta$-1, 3 glucan and Baism affected growth, feed efficiency, specific growth rate, protein efficiency ratio, Peak value of CL and Lysozyme activity, and the optimum dietary supplementation level of $\beta$-1, 3 glucan and Baism as a feed additive could be approximately $\beta$-1, 3 glucan 0.10% ＋ Baism 0.90% of diet in juvenile olive flounder (Paralichthys olivaceus).

• 한국작물학회지
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• 제42권4호
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• pp.403-409
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• 1997

취반용 보리 종실 내 (1-3, 1-4)-$\beta$-glucan의 축적기작을 알아보기 위하여 개화 후 일정 간격으로 보리 종실을 채취하여 (1-3, 1-4)-$\beta$-glucan 함량, (1-3, 1-4)-$\beta$-glucanase 활성 및 전분함량과 $\alpha$-amylase 활성을 조사하였다. $\beta$-glucan 함량은 등숙 초기에 매우 낮았으나 개화 후 15~25일 사이에 급속히 증가하였고 개화 후 30일경에는 종실 건물중의 3.5~4%로 증가하였다. (1-3, 1-4)-$\beta$-glucanase 활성은 등숙 초기에 높았으며 등숙이 진전될수록 감소하였다. $\beta$-glucan 함량과 (1-3, 1-4)-$\beta$-glucanase 활성간에는 고도로 유의한 부의 상관관계가 인정되었으나 등숙 종실 중의(1-3, 1-4)-$\beta$-glucanase 활성이 매우 낮은 수준이므로 $\beta$-glucan 축적량에 크게 영향을 미치지 않을 것으로 추정되었다. 전분함량은 등숙이 진전됨에 따라 지속적으로 증가하였으며 개화 후 10~20일에 가장 뚜렷하게 증가하였다. 포도당함량은 등숙이 진전됨에 따라 현저히 감소하였으며, 등숙 초기와 중기에 감소 정도가 매우 컸다. $\alpha$-amylase의 활성도 등숙이 진전됨에 따라 현저히 감소하였으며, 감소 정도는 등숙 초기와 중기에 매우 컸다.

• 약학회지
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• 제54권6호
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• pp.441-448
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• 2010

In present study, classification and quality control of Genus Polygonatum were developed using the isolated from Polygonati Odorati Rhizoma and Polygonati Rhizoma. 3 components were isolated from Butanol fractions of Polygonati Rhizoma, and 2 components were isolated from Hexane and Butanol fractions of Polygonati Odorati Rhizoma. All the components were obtained using silica gel and ODS column chromatography. The compounds were identified as adenosine, 14-hydroxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-D-galactopyranosyl-26-O-${\beta}$-D-glucopyranoside, 22-O-methyl-14-hydrocxyfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-Dgalactopyranosyl-26-O-${\beta}$-D-glucopyranoside, ${\beta}$-Sitosteryl-3-O-${\beta}$-D-D-glucopyranoside, 14-hydoxylfurost-5-ene-3-O-${\beta}$-Dglucopyranosyl-($1{\rightarrow}2$)-O-[${\beta}$-D-xylopyranosyl-($1{\rightarrow}3$)]-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-D-galactopyranoside through physicochemical data, spectroscopic methods ($^1H$-NMR, $^{13}C$-NMR, Mass) according references. The quality control of genus Polygonatum were conducted using HPLC quantitative analysis of 14-hydroxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-${\beta}$-D-glucopyranosyl-($1{\beta}4$)-O-${\beta}$-D-galactopyranosyl-26-O-${\beta}$-D-glucopyranoside, 14-hydoxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-[${\beta}$-D-xylopyranosyl-($1{\rightarrow}3$)]-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-D-galactopyranoside in 30 samples collected throughout Korea and China. This method provided a tool for standardization of mix or misusing the commercial Odorati Rhizoma and Polygonati Rhizoma. As a result, contained quantity of 14-hydroxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-D-galactopyranosyl-26-O-${\beta}$-D-glucopyranoside was measured $0.008{\pm}0.006%$ and 14-hydoxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-[${\beta}$-D-xylopyranosyl-(13)]-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-Dgalactopyranoside was measured $0.026{\pm}0.012%$.

• Archives of Pharmacal Research
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• 제22권4호
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• pp.423-427
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• 1999

A study was carried out to evaluate flavonol glycosides in leaves of Symplocarpus renifolius (Araceae). From the water fraction of the MeOH extract, three new flavonol glycosides were isolated along with three known compounds, Kaempferol-3-O-$\beta$-glucopyranosyl-($1{\rightarrow}2$)-$\beta$-D-glucopyranosyl-7-O-$\beta$-D-glucopyranoside, quercetin-3-O-$\beta$-D-glucopyranosy-1-($1{\rightarrow}2$)-$\beta$-D-glucopyranoside, and caffeic acid. The structures of the new flavonol glycosides were elucidated by chemical and spectral analyses a quercetin-3-O-$\beta$-D-glucopyranosyl-($1{\rightarrow}2$)-$\beta$-D-glucopyranosyl-7-O-$\beta$-D-glucopyranoside, isorhamnetin-3-O-$\beta$-D-glucopyranosyl-(1 2)-$\beta$-D-glucopyranosyl-7-O-$\beta$-D-glucopyranosdie, and quercetin-3-O$\beta$-D-glucopyranosyl-($1{\rightarrow}2$)-$\beta$-D-glycopyranosyl-7-O-($6^{IIII}$-trans-caffeoyl)-$\beta$-D-glucopyranoside.