• Proceedings of the Korean Society of Life Science Conference
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• 한국생명과학회 2007년도 제48회 학술심포지움 및 추계국제학술대회
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• pp.109.1-109.1
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• 2007

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• The Korean Journal of Physiology and Pharmacology
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• 제12권5호
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• pp.231-236
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• 2008

Heparin is a well-known anticoagulant widely used in various clinical settings. Interestingly, recent studies have indicated that heparin also has anti-inflammatory effects on neuroinflammation-related diseases, such as Alzheimer's disease and meningitis. However, the underlying mechanism of its actions remains unclear. In the present study, we examined the anti-inflammatory mechanism of heparin in cultured cerebral endothelial cells (CECs), and found that heparin inhibited the tumor necrosis factor $\alpha$ ($TNF{\alpha}$)-induced and nuclear factor kappa B (NF-${\kappa}B$)-dependent expression of adhesion molecules, such as intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), which are crucial for inflammatory responses. Heparin selectively interfered with NF-${\kappa}B$ DNA-binding activity in the nucleus, which is stimulated by $TNF{\alpha}$. In addition, non-anticoagulant 2,3-O desulfated heparin (ODS) prevented NF-${\kappa}B$ activation by $TNF{\alpha}$, suggesting that the anti-inflammatory mechanism of heparin action in CECs lies in heparin's ability to inhibit the expression of cell adhesion molecules, as opposed to its anticoagulant actions.

• Parasites, Hosts and Diseases
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• 제55권4호
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• pp.375-384
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• 2017

Trichomonas vaginalis is a pathogen that triggers severe immune responses in hosts. T. vaginalis ${\alpha}$-actinin 2, $Tv{\alpha}$-actinin 2, has been used to diagnose trichomoniasis. This study was undertaken to examine the role of $Tv{\alpha}$-actinin 2 as an antigenic molecule to induce immune responses from humans. Western blot analysis using anti-$Tv{\alpha}$-actinin 2 antibodies indicated its presence in the secreted proteins of T. vaginalis. ELISA was employed to measure cytokine production by vaginal epithelial cells, prostate cells, mouse dendritic cells (DCs), or T cells stimulated with T. vaginalis or $Tv{\alpha}$-actinin 2 protein. Both T. vaginalis and $rTv{\alpha}$-actinin 2 induced cytokine production from epithelial cell lines, including IL-10. Moreover, $CD4^+CD25^-$ regulatory T cells (Treg cells) incubated with $rTv{\alpha}$-actinin 2-treated DCs produced high levels of IL-10. These data indicate that $Tv{\alpha}$-actinin 2 modulates immune responses via IL-10 production by Treg cells.

• Bulletin of the Korean Chemical Society
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• 제21권6호
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• pp.562-566
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• 2000

$\beta-D-Mannopyranosyl$ disaccharides have been obtained from the coupling of 4,6-O-benzylidene derivatives of ethylthio $\alpha-D-mannopyranoside$ employing NIS-TfOH promoter. NIS-TfOH promoted couplings of the corresponding ethylthio $\beta-D-glucopyranoside$ and produced $\alpha-D-glucopyranosyl$ disaccharides. IDCP (iodonium dicollidine perchlorate) was inactive toward the 4,6-O-benzylidenated ethylthio glucopyranosyl donor. However,lDCP coupled the 4,6-O-benzylidenated $ethylthio-\beta-D-galactopyranoside$ to give a-D-galactopyranosyl disaccharides.

• Korean Journal of Animal Reproduction
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• 제25권2호
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• pp.155-162
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• 2001

These experiments were conducted to evaluate the effects of the administration of exogenous PG $F_{2{\alpha}}$ on hormone concentrations and follicular development in early postpartum(pp) Korean native goats. 1. Plasma PG $F_{2{\alpha}}$ concentrations in PG $F_{2{\alpha}}$ treated goals showed a gradual increase from a low on day 2 (GR-1 : 10 day-treatment group: 6.35$\pm$0.5 and GR-2; 4 day-treatment group: 0.22$\pm$0.2 pg/$m\ell$, respectively) to reach a peak of 21.18$\pm$1.6 or 4.21 $\pm$0.4 pg/$m\ell$ on day 4 after treatment of PG $F_{2{\alpha}}$. 2. Plasma PG $F_{2{\alpha}}$ concentrations in GR-1 goats averaged 9.08 $\pm$1.2 pg/$m\ell$ compared with 5.44$\pm$ 1.8 pg/$m\ell$ in GR-2 goats the day before treatment of PG $F_{2{\alpha}}$. Mean PG $F_{2{\alpha}}$ concentrations thereafter remained low during the treatment period but PG $F_{2{\alpha}}$ concentrations did not differ between the two group. 3. Plasma concentrations of estradiol-17 $\beta$ (E,) in PG $F_{2{\alpha}}$ - treated group were decreased gradually until 2 days after PG $F_{2{\alpha}}$ treatment but mean $E_2$ concentrations began to increase on 3 days and were Inaximal on the 4 days after treatment. 4. Plasma lulenizing hormone (LH) concentrations in PG $F_{2{\alpha}}$ - treated goats were slightly higher than in controls but mean LH concentrations did not differ between the two treatment groups. 5. Plasma prolactin (PRL) concentrations were suppressed in both GR-1(10 day-treatment group) and GR-2(4 day-treatment group) goats compared to saline controls but mean PRL concentrations remained lower in PG $F_{2{\alpha}}$ treated animals during post-treatment period. 6. The mean number of small and medium follicles present when PG $F_{2{\alpha}}$ was administrated was similar in all does but the increase in number of large follicles($\geq$4mm) tended to be higher in PG $F_{2{\alpha}}$ treated group than controls. These results suggest that concentrations of PG $F_{2{\alpha}}$ and estradiol-17$\beta$ were positively correlared with follicular diameter. We conclude that PG $F_{2{\alpha}}$ treatment stimulates follicular development similarly in both GR-1 and GR-2 group.

• Bulletin of the Korean Chemical Society
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• 제30권9호
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• pp.2039-2042
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• 2009

As a part of an ongoing effort to discover inhibitors of the Hepatitis C Virus RNA replication, we describe here the first synthetic route of 1'($\alpha$),2'($\beta$)-C-dimethyl carbocyclic adenine analogue. The key intermediate cyclopentenyl alcohol 8($\alpha$) was prepared from aldehyde 4 using ring-closing metathesis (RCM) as a key reaction. Coupling of 8($\alpha$) with nucleosidic base via the regioselective Mitsunobu reaction followed by stereoselective dihydoxylation and deprotection afforded the target carbocyclic adenine analogue 12.

• The Bulletin of The Korean Astronomical Society
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• 제42권2호
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• pp.43.1-43.1
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• 2017

A three-dimensional (3D) $Ly{\alpha}$ radiative transfer code is developed to study the Wouthuysen-Field effect, which couples the 21 cm spin temperature of neutral hydrogen and the $Ly{\alpha}$ radiation field, and the escape fraction of $Ly{\alpha}$ from galaxies. The Monte Carlo code is capable of treating arbitrary 3D distributions of $Ly{\alpha}$ source, neutral hydrogen and dust densities, gas temperature, and velocity field. It is demonstrated that the resonance-line profile at the center approaches to the Boltzmann distribution with the gas temperature. A plane-parallel ISM model, which is appropriate for the neutral ISM of our Galaxy, is used to calculate the $Ly{\alpha}$ radiation field strength as a function of height above the galactic plane. We also use a two-phase, clumpy medium model which is composed of the cold and warm neutral media (WNM). It is found that the $Ly{\alpha}$ radiation field is strong enough to thermalize the 21 cm spin temperature in the WNM to the gas kinetic temperature. The escape fraction of $Ly{\alpha}$ is found to be a few percent, which is consistent with the $Ly{\alpha}$ observations of our Galaxy and external galaxies.

• Korean Journal of Mathematics
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• 제21권4호
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• pp.429-437
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• 2013

Let $X_1$, $X_2$, ${\cdots}$, $X_n$ be independent and identically distributed random variables having common exponential density with unknown mean ${\mu}$. In the sequential confidence interval estimation for the exponential hazard rate ${\theta}=1/{\mu}$, when the loss function is strictly convex, the following stopping rule is proposed with the half length d of prescribed confidence interval $I_n$ for the parameter ${\theta}$; ${\tau}$ = smallest integer n such that $n{\geq}z^2_{{\alpha}/2}\hat{\theta}^2/d^2+2$, where $\hat{\theta}=(n-1)\bar{X}{_n}^{-1}/n$ is the minimum risk estimator for ${\theta}$ and $z_{{\alpha}/2}$ is defined by $P({\mid}Z{\mid}{\leq}{\alpha}/2)=1-{\alpha}({\alpha}{\in}(0,1))$ Z ~ N(0, 1). For the confidence intervals $I_n$ which is required to satisfy $P({\theta}{\in}I_n){\geq}1-{\alpha}$. These estimated intervals $I_{\tau}$ have the asymptotic consistency of the sequential procedure; $$\lim_{d{\rightarrow}0}P({\theta}{\in}I_{\tau})=1-{\alpha}$$, where ${\alpha}{\in}(0,1)$ is given.

• Bulletin of the Korean Chemical Society
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• 제27권1호
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• pp.93-98
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• 2006

Two new compounds 1'-(stigmast-11,20(21),25-trien 3$\alpha$,9$\alpha$-diolyl)-3'-(pimara-11,15-dien-3$\alpha$-olyl) glycerol (1) and stigmast-5-en-3$\alpha$,26-diol (2) along with known fatty acids n-hexacosanoic acid (3) and hexadecanoic acid (4), have been isolated from the methanol extract of rice hulls of Oryza sativa. The structures of the compounds were elucidated using 1D and 2D NMR spectral methods, viz; $^1H, ^{13}C, ^{13}C$-DEPT, $^1H-^1H$ COSY, $^1H-^{13}C$ HETCOR, HSQC and HMBC aided by IR, EIMS, FABMS and HRFABMS. Compound (1) showed inhibition to radish germination, growth of shoot and root length.

• Archives of Pharmacal Research
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• 제27권7호
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• pp.776-780
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• 2004

Interferons are cytokines that confer resistance to viral infection and inhibit cellular proliferation. The interferon alpha gene from human blood samples was amplified, cloned and expressed in E. coli (BL21). Leukocyte chromosomal DNA was used as a source of template DNA. Using specific primers, the gene for HulFN$\{alpha}$-2b was amplified and inserted into the E. coli vector, pET21b, by ligation of the HindIII and BamHI linkers of the vector and insert. The insert was further analyzed by PCR, DNA restriction mapping and sequencing, and expressed in a suitable E. coli strain. The production of this important cellular protein in the laboratory has significant applications in production of the recombinant pharmaceutical proteins.