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Mycelial Production and Amylase Activity of Fungi for Brewing in Different Submerged Culture Conditions (액체배양에서의 양조용 곰팡이의 균체 생산 및 전분분해효소 활성)

  • Noh, Jong-Min;Choi, Ji-Ho;Jung, Seok-Tae;Yeo, Soo-Hwan;Park, Jang-Woo;Lee, Jin-Won;Choi, Han-Seok
    • Journal of the East Asian Society of Dietary Life
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    • v.23 no.6
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    • pp.833-838
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    • 2013
  • In this study, twelve strains of brewing fungi were individually cultivated on wheat extract broth (WEB), potato dextrose broth (PDB) and malt extract broth (MEB) in order to determine the microorganism with good culture characteristics as well as with amylolytic activity. The strain cultured in PDB exhibited mycelia production from 12.6 g/L (Rhizopus oryzae KACC 45714) to a maximum of 48.0 g/L (Aspergillus oryzae KACC 46959), which was 2.3~9.2 times lower than that of the strain cultured in WEB and 1.7~14.6 times lower than that of the strain cultured in MEB. Accorfing the results, We found that the commercial strains of A. oryzae Suwon, CF1001 and CF1003 had a higher dry cell mass than the wild-type strains KACC 46421, 46423, 46424 and 46959. For Rhizopus sp., the acidity levels in WEB, PDB and MEB were 0.12~0.47%, 0.22~1.0% and 0.16~0.68% (equivalent lactic acid concentration) respectively. For A. oryzae, the acidity levels were 0.06~0.11%, 0.03~0.04% and 0.06~0.08% (equivalent lactic acid concentration), respectively. Amylase enzyme from Rhizopus delemar KACC 46419 exhibited an enzyme activity of 0.013 U and 0.019 U in WEB and MEB cultures, respectively. The enzyme activity of the amylase enzyme from A. oryzae was 0.019~0.037, 0.017~0.033 and 0.028~0.046 U in WEB, PDB and MEB cultures, respectively.

The Accommodative Lag and Refractive Error in Early Adults (초년 성인의 굴절이상과 조절래그 분포의 연관성)

  • Baarg, Saang-Bai;Jeong, Youn Hong
    • Journal of Korean Ophthalmic Optics Society
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    • v.17 no.1
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    • pp.59-65
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    • 2012
  • Purpose: The purpose of this study was to determine the distribution and correlation of accommodative lag with refractive error. Method: We had tested the clinical refraction and the accommodative lag in clinically normal 49 young adults (total 98 eyes) aged 18 to 25 years without abnormal binocular function. Monocular and binocular accommodative lag were tested with 0.50 D cross-cylinder lens and near vision test chart which had cross-hairs after full correction of LogMAR visual acuity over 0.05. Results: There was no statistical differences in monocular accommodative lag between right ($0.64{\pm}0.64$ D) and left eye ($0.63{\pm}0.64$)(p=0.858). The accommodative lag of male was higher than female and the range of the value was broader than female in binocular accommodative lag (p=0.015). The wider the inter-pupillary distance was, the higher the accommodative lag was (p=0.003). However, there were no differences with age (p=0.800) and dominant eye (p=0.402). The ranges of accommodative lag of low, middle, and high myopia were 0.75 ~ -0.25 D, 1.25 ~ -0.50 D, and 1.50 ~ -0.75 D, respectively, and the regression was 'y = -0.03953x+0.09205'. Conclusions: These data suggest that clinically normal young adults with high amounts of refractive error have more variable accommodative lag and increased spherical equivalent refraction.

Trace Metal Contents in Fishes and Shellfishes and Their Safety Evaluatins (우리나라 어패류중 미량금속 함량 및 안전성 평가)

  • 소유섭;김정수;정소영;김미혜;홍무기
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.4
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    • pp.549-554
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    • 2000
  • This study was carried out to estimate the contents of trace metals in the fishes and shellfishes available on Korean markets. The maples were digested with acids, then analyzed by ICP (INductively Coupled Plasma Spectrometer) and AAS (Atomic Absorption Spectrometer) for the contents of lead (Pb), cadmium (Cd), arsenic (As), copper (Cu), manganese (Mn) and zinc (Zn). The contents of mercury (Hg) were determined using a mercury analyzer. The contents of trace metals [minimum~ maximum (mean), mg/kg] in fishes were as follows; Hg;0.004~0.500(0.082), Pb;N.D.~ 1.87 (0.29), Cd;N.D. ~0.094 (0.020), As;0.01~5045 (0.84), Cu;0.04~3.74 (0.66), Mn; N.D. ~7.56 (0.57), Zn;0.92~18.33(6.03). Those contents in shellfishes were as follows (mg/kg); Hg;N.D.~0.221 (0.029), Pb;0.01~1.51 (0.38), Cd;0.02~1.93 (0.51), As;0.18~3.07 study showed that trace metal contents in both fishes and shellfishes on our markets of lead, cadmium and mercury from fishes and shellfishes takes about 1~13% of PTWI(Provisional Tolerable Weekly Intakes) that FAO/WHO Joint Food Additive and Contaminants Committee has set to evaluate their safeties.

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Cell Properties for SOFC Using Synthesized Powder of Electrolyte LSGM System and Cathode LSM System (LSGM 전해질과 LSM 양극의 합성분말을 이용한 SOFC 단위전지의 특성)

  • Lee, Mi-Jai;Nam, Jeong-Hee;Choi, Byung-Hyun
    • Journal of the Korean Ceramic Society
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    • v.39 no.4
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    • pp.359-366
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    • 2002
  • The purpose of this study is to investigate the properties of LSGM electrolyte and LSM cathode. The unit cell based on the optimum conditions and processing for high performance was fabricated and measured. The single phase of $LaGaO_3$ was obtained on sintering at $1500^{\circ}$ for 6h with composition of $(La_{0.85}Sr_{0.15})(Ga_{0.8}Mg_{0.2})O_{3-\delta}와 (La_{0.8}Sr_{0.2})(Ga_{0.8}Mg_{0.2})O_{3-\delta}$ and $(La_{0.85}Sr_{0.15})(Ga_{0.8}Mg_{0.2})O_{3-\delta}$. The grain size of the sintered body was about $10∼30{\mu}m$ and electrical conductivity was 0.13 S/cm measured at $800^{\circ}$. The single phase of $LaMnO_3$ structure in $(La1-xSrx)MnO_3$ system was obtained at x=0∼0.2 and the particle size of the synthesized powder was about 40 nm. The unit cell was prepared by firing at $1200^{\circ}$ for 1h with $(La_{0.9}Sr_{0.1})MnO_3$ cathode and 0.9NiO-0.1YSZ anode screen-printed on surfaces of $(La_{0.8}Sr_{0.2})(Ga_{0.8}Mg_{0.2})O_{3-\delta}$ electrolyte. The grain size of the electrode was close to $1{\mu}m$ and the electrode had porous structure. The maximum power density of unit cell showed $0.3W/cm^2$ at $800^{\circ}$.

A comparative Study on the Activities of Glutamic Dehydrogenase and Glutamic Transaminase in Livers of the Crucian carp, Pigeon, and Rat (붕어, 비둘기, 흰쥐의 肝臟의 Glutamic Dehydrogenase 및 Glutamic Transaminase 의 活性에 관한 比較 硏究)

  • Kim, Yong Kyu;Nam, Sang Yul
    • The Korean Journal of Zoology
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    • v.12 no.2
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    • pp.50-56
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    • 1969
  • The present investigation involves a comparative study of enzymatic activities in various animals. The levels of the liver protein of rat (22.0$\pm$0.01mg/ml and pigeon (22.0$\pm$ 0.16) are twice as high as that of crucian carp (13.0$\pm$ 0.09)(p < 0.01). Generally, the specific acitivity (3.77 $\pm$0.18 unit/mg) of rat glutamic pyruvic transaminase (GPT) is highest, pigeon intermediate (1.93 $\pm$0.01), and crucian carp lowest (0.71$\pm$0.07). On the other hand, the specific activity (8.23$\pm$0.09 unit/mg)of rat glutamic oxaloacetic transaminase (GOT) is highest, pigeon intermediate (3.95$\pm$0.09), and crucian carp lowest (0.92$\pm$0.01) (p < 0.01). Ratios of GOT activity to GPT activity appear no remarkable difference from the levels of various animals. Specific activity of glutamic dehydrogenase(GDH) in pigeon tissue exceeds those of rat and crucian carp. In liver, rat GOT specific activity is greater than crucian carp and pigeon. On the other hand, pigeon GDH specific activity is greater than those of rat and crucian carp. This wouls seem to be in accord with protein metabolic intensity. The patterns for GDH isozyme were remarkably appeared in various animals. Glutamic dehydrogenase isozymes gave different electrophoretic mobilities in various animals. It is interesting that crucian carp, pigeon, and rat would show this difference, which may be indicative of an evolutional pattern. The fact that livers in various animals show quite different enzyme activities would suggest the existence of such a general phylogenetic relationship.

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Fabrication and characteristics of modified PZT System doped With $La_2O_3$ ($La_2O_3$가 첨가된 modified PZT계의 제조 및 특성)

  • 황학인;박준식;오근호
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.7 no.3
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    • pp.418-427
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    • 1997
  • The effect of $La_2O_3$ as a dopant on the microstructure structure, crystal structure and electrical properties was studied. $0.05Pb(Sn_{0.5}Sb_{0.5})O_3+0.11PbTiO_3+0.84PbZroO_3+0.4Wt%MnO_2$ (=0.05PSS +0.11PT+0.84PZ+0.4wt%$MnO_2$) systems doped with 0, 0.1, 0.3, 0.5, 0.7, 1, 3, 5 mole% $La_2O_3$ were fabricated and investigated sintering density, crystal structure and micro-structure. The sintered 0.05PSS+0.11PT+0.84PZ+0.4wt%$MnO_2$ system doped with $La_2O_3$showed sintering density of the range of 7.683 g/㎤ of 0 mole% doping to 7.815 g/㎤ of 0 mole% doping. The average grain sizes in the range of 0 to 5 mole% $La_2O_3$were decreased from 9.0 $\mu\textrm{m}$ to 1.3 $\mu\textrm{m}$. X-ray diffraction investigation of sintered bodies showed that solid solutions were formed between 0.05PSS+0.11PT+0.84PZ+0.4wt%$MnO_2$ system and $La_2O_3$ in the range of 0 to 1 mole% but second phases were formed in case of 3, 5 mole%. Dielectric constants at 1 kHz were increased with 0 to 3 mlole% $La_2O_3$ before and after poling at the condition of 5 $KV_{DC}$/mm at $120^{\circ}C$ or $140^{\circ}C$ during 20 minutes. All Dielectric losses at 1 kHz were less than 1%, Curie temperatures were $208^{\circ}C$, $183^{\circ}C$, $152^{\circ}C$ and $127^{\circ}C$ at 0, 0.5, 1, 3 mole% $La_2O_3$ respectively. The values of $K_p$ were increased from 0 to 3 mole% $La_2O_3$ after poling at condition of 5 $KV_{DC}$mm at the condition of $120^{\circ}C$ or $140^{\circ}C$. The case of 0.7 mole% $La_2O_3$doped 0.05PSS+0.11PT+0.84PZ+0.4wt%$MnO_2$ system showed $K_p$ of 14.5% by poling at $140^{\circ}C$ during 20 minutes.

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Analysis of Microsatellite DNA Polymorphism for Parentage Testing in Dog Breeds (개의 친자감정을 위한 Microsatellite DNA 다형성 분석)

  • Cho, G. J.;Cho, B. W.;Kim, S. K.;Lee, K. W.;Kim, Y. K.
    • Journal of Animal Science and Technology
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    • v.45 no.2
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    • pp.191-198
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    • 2003
  • This study was carried out to investigate a usefulness of the microsatellite DNA markers for individual identification and parentage verification in three dog breeds. A total of 59 random dog (31 Chiwawa, 20 Poongsan, 8 Labrador Retriever) samples were genotyped by using 14 markers (Chiwawa dog), 16 markers (Poongsan dog), and 12 markers (Labrador Retriever dog) among the 17 international standard markers (PEZ1, 3, 5, 6, 8, 10, 11, 12, 13, 15, 16, 17, 20, 21, FHC2010, FHC2054 and FHC2079), respectively. The number of alleles per locus varied from 4 to 14 with a mean value of 6.07 in Chiwawa dog, 2 to 9 with a mean of 4.75 in Poongsan dog, and 3 to 5 with a mean of 4.00 in Labrador Retriever dog. Observed heterozygosity was ranged 0.419${\sim}$0.968 (mean 0.755), 0.300${\sim}$0.950 (mean 0.597) and 0.125${\sim}$0.750 (mean 0.604), and expected heterozygosity was ranged 0.432${\sim}$0.883 (mean 0.711), 0.262${\sim}$0.817 (mean 0.559) and 0.425${\sim}$0.808 (mean 0.660) in these three dog breeds. PIC value was ranged 0.397${\sim}$0.856 (mean 0.659), 0.222${\sim}$0.772 (mean 0.503) and 0.354${\sim}$0.717 (mean 0.563) in these three dog breeds. Of the 17 markers, PEZ1, PEZ3, PEZ6, PEZ10, PEZ12 loci, PEZ1, PEZ6, PEZ13 loci, and PEZ8, PEZ12 loci have relatively high PIC value (>0.7) in Chiwawa dog, Poongsan dog and Labrador Retriever dog, respectively. The exclusion probability was ranged 0.240${\sim}$0.741, 0.111${\sim}$0.616, and 0.198${\sim}$0.529, and the combination of microsatellite loci was 0.9999, 0.9991, and 0.9968 in Chiwawa dog, Poongsan dog and Labrador Retriever dog, respectively. These results can give basic information for developing parentage verification and individual identification system in these three dog breeds.

Evaluation of Target Position's Accuracy in 2D-3D Matching using Rando Phantom (인체팬톰을 이용한 2D-3D 정합시 타켓위치의 정확성 평가)

  • Jang, Eun-Sung;Kang, Soo-Man;Lee, Chul-Soo
    • The Journal of Korean Society for Radiation Therapy
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    • v.21 no.1
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    • pp.33-39
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    • 2009
  • Purpose: The aim of this study is to compare patient's body posture and its position at the time of simulation with one at the treatment room using On-board Imaging (OBI) and CT (CBCT). The detected offsets are compared with position errors of Rando Phantom that are practically applied. After that, Rando Phantom's position is selected by moving couch based on detected deviations. In addition, the errors between real measured values of Rando Phantom position and theoretical ones is compared. And we will evaluate target position's accuracy of KV X-ray imaging's 2D and CBCT's 3D one. Materials and Methods: Using the Rando Phantom (Alderson Research Laboratories Inc. Stanford. CT, USA) which simulated human body's internal structure, we will set up Rando Phantom on the treatment couch after implementing simulation and RTP according to the same ways as the real radioactive treatment. We tested Rando Phantom that are assumed to have accurate position with different 3 methods. We measured setup errors on the axis of X, Y and Z, and got mean standard deviation errors by repeating tests 10 times on each tests. Results: The difference between mean detection error and standard deviation are as follows; lateral 0.4+/-0.3 mm, longitudinal 0.6+/-0.5 mm, vertical 0.4+/-0.2 mm which all within 0~10 mm. The couch shift variable after positioning that are comparable to residual errors are 0.3+/-0.1, 0.5+/-0.1, and 0.3+/-0.1 mm. The mean detection errors by longitudinal shift between 20~40 mm are 0.4+/-0.3 in lateral, 0.6+/-0.5 in longitudinal, 0.5+/-0.3 in vertical direction. The detection errors are all within range of 0.3~0.5 mm. Residual errors are within 0.2~0.5 mm. Each values are mean values based on 3 tests. Conclusion: Phantom is based on treatment couch shift and error within the average 5mm can be gained by the diminution detected by image registration based on OBI and CBCT. Therefore, the selection of target position which depends on OBI and CBCT could be considered as useful.

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A Study on the Metabolism of Riboflavin in Korean Men (한국인 성인남자의 리보플라빈 대사에 관한 연구)

  • 황금희
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.4
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    • pp.594-603
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    • 1994
  • This study investigate the balance and biochemical status of riboflavin in Korean men. During the experimental period, four riboflavin diets with different levels of riboflavin(0.4, 0.6,0.8 and 1.0mg/1000kcal) were followed by eight healthy college men. the riboflavin status was assayed by erythrocyte glutathine reudcctase activity coefficient (EGRAC0 and urinary excretion of riboflavin. Riboflavin intake of the subjects who consumed a det was 0.46mg/1000kcal. the riboflavin intakes of the subjects who consumed the experimental diet with 0.4, 0.6, 0.8 and 1,0mg/1000kcal of riboflavin were 0.41, 0.60, 0.81 , 0.97mg, respectively. Fecal riboflavin loss, absorbed riboflavin , urinary riboflavin loss and retained riboflavin increased in the subjects consumed 0.4, 0.6, 0.8 and 1.0mg/1000kcal of riboflavin. The average EGRAC values for the subjects consumed 0.4, 0.6, 0.8 and 1.0mg/1000kcal of riboflavin were 1.303$\pm$0.029, 1.271$\pm$0.022, 1.239$\pm$0.013, 1.202$\pm$0.030, respectively and urinary riboflavin values ($\mu\textrm{g}$/g creatinine) were 86.89$\pm$ 20.07, 123.88$\pm$ 15.88, 240.70$\pm$57.14 and 393.36$\pm$76.94, respectively. Results indicate that 0.6mg/1000kcal is the level of riboflavin intake needed to maintain urinary riboflavin within the normal range. And above1.0mg/1000kcal of riboflavin is need to maintain urinary riboflavin with in the normal range. And above 1.0mg/1000 of riboflavin is needed to maintain the EGRAC within the normal range. The riboflavin intake correlated positively with urinary riboflavin value, but correlated negatively with the EGRAC value. The EGRAC value correlated negatively to protein intake as well as animal protein intake. The linear equation of between riboflavin intake and EGRAc was EGRA=-0.1667 $\times$riboflavin intake +1.3710. The riboflavin intake to maintain EGRAc below 1.20 was calculated 1.02mg/1000kcal by the above equation.

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Determination of Bioconcentration Factor on BPMC and Carbaryl in Carassius auratus(goldfish) (Carassius auratus(goldfish)를 이용한 BPMC와 Carbaryl의 생물농축계수의 측정)

  • 박선열;민경진;강회양
    • Journal of Environmental Health Sciences
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    • v.20 no.1
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    • pp.75-82
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    • 1994
  • Bioconcentration Factor (BCF) is known as important criteria for ecotoxicology on hazardous chemicals. But there is no standard method for determining BCF and reported BCFs were slightly different in accordance with authors. This study was performed with aims to determine BCFs on BPMC and Carbaryl. Carassius auratus(goldfish) be chosen as test organism and test period were 3-day, 5-day and 10-day. Extract solvents were n-hexane and acetonitrile. GC-ECD was used to detecting carbamates. The obtained results were as follows: 1. It was possible to determine short term BCF$_s$ of Carbaryl or BPMC through relatively simple procedure. 2. BCF$_3$ of Carbaryl in concentration of 1, 2, 5, 10 ppm were 0.34 $\pm$ 0.06, 0.18 $\pm$ 0.02, 0.10 $\pm$ 0.01, 0.06 $\pm$ 0.01 respectively. BCF$_5$ of Carbaryl were 0.34 $\pm$ 0.05, 0.18 $\pm$ 0.02, 0.13 $\pm$ 0.01 and 0.07 $\pm$ 0.01, BCF$_{10}3$ of Carbaryl were 0.45 $\pm$ 0.05, 0.27 $\pm$ 0.02, 0.16 $\pm$ 0.02 and 0.09 $\pm$ 0.01. BCF$_3$ of BPMC in concentration of 1, 2, 5 ppm were 4.66 $\pm$ 0.17, 2.64 $\pm$ 0.49, 1.88 $\pm$ 0.24 respectively. BCF$_5$ of BPMC were 4.09 $\pm$ 0.50, 2.42 $\pm$ 0.37 and 1.83 $\pm$ 0.15. 3. BCF$_s$ of BPMC were decreased as increasing concentration. However, BPMC concentration in fish were increased in contrast to BCF. But more concentrated BPMC was found in fish 3-day test than found concentration in fish 5-day test. 4. Same trend appeared in Carbaryl. BCF$_s$ of Carbaryl were decreased as increasing concentration and prolonging test period. But found Carbaryl concentration in fish were increased. 5. BCF$_s$ of BPMC were higher than that of Carbaryl by 10 times, in spite of the physicochemical properties of the two carbamates were similar to each other. Further study is recommended to find out the reason of the difference.

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