• Journal of the Korean Magnetic Resonance Society
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• v.25 no.1
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• pp.1-7
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• 2021

Employing of 13C stable-isotopes in NMR metabolomics can give unique splitting patterns and coupling constants information originated from 13C-13C coupling interaction that provide an important structural information regarding the cellular metabolic process. But it has been known that an undesirable signal distortion in 2D heteronuclear correlation study, due to 13C-13C interaction, hampers an analysis of the coupling information. Recently, we proposed J-scaled distortion-free heteronuclear single-quantum coherence (HSQC) sequence which provides a distortion-free 13C-13C coupling information with a selective resolution enhancement of JCC splitting. In this paper, we dicuss theoretical aspect and practical feature of J-scaled HSQC pulse sequence. The conceptual explanation of orgin of the signal distortion by 13C-13C coupling interaction and design of J-scaled HSQC through exemplified results are provided in brief.

• Bulletin of the Korean Chemical Society
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• v.21 no.11
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• pp.1159-1161
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• 2000

• Bulletin of the Korean Chemical Society
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• v.19 no.3
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• pp.295-299
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• 1998

HSP104 protein in Saccharomyces cerevisiae is known to provide thermotolerance when induced by various kinds of stresses, such as a mild heat shock, ethanol, and hypoxia. It helps cells survive at an otherwise lethal temperature. Mechanisms by which HSP104 protein works are yet to be elucidated. In order to understand a molecular basis of thermotolerance due to HSP104 protein induced by a mild heat shock, studies on respiratory pathways were carried out in the wild type as well as in the hsp104 deleted mutant. Especially the degree of 13C-acetate incorporation into glutamate-C4 was examined for both strains using 13C-13C homonuclear spin coupling measurements, since glutamate is in a rapid equilibrium with α-ketoglutarate in the TCA cycle. In addition, the temperature effects on the rate of 13C incorporation are compared with or without HSP104 protein expressed. Finally, the inhibitory effect of HSP104 on the respiration pathway was confirmed by the measurements of oxygen consumption rates for both strains.

• Korean Journal of Pharmacognosy
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• v.8 no.1
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• pp.23-29
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• 1977

The $^{13}C-chemical\;shifts$ of the phenolic coumarine derivatives, aesculetin, daphnetin and herniarin were studied on the basis of my previous report. All spectral data found in this report could be utilized to the structure elucidation of the unknown phenolic coumarine derivatives and other phenolic compounds. In addition, it is suggested that a long range coupling may occur in the following structure as represented by arrow.

• Archives of Pharmacal Research
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• v.17 no.6
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• pp.487-489
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• 1994

A flavone glycoside was isolated from the leaves of Betula platyphylla var. latifolia and characterized as $4, 6-Dimethoxy-5-hydroxyflavone-7-O-{\beta}-D-glucoside(pectolinarigenin-7-O-{\beta}-D-glucopy-ranoside)$ by method of chemical and NMR spectral analysis. $^13C-^1H$ long range coupling was confirmative for determination of its substituted position. In connection with this study, 6-C-Glucosylnalingenin and 6-C-Glucosylaromadendrin were confirmed its structures using this technique.

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.35 no.10
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• pp.181-190
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• 2019

In this study, four reinforced concrete coupling beams were subjected to cyclic lateral loading test to evaluate the structural performance of coupling beam according to volume fraction of steel fiber. For this purpose, the volume fraction of steel fiber(0%, 1%, 2%) and transverse reinforcement spacing were determined as the main parameter. According to the test results, the maximum strength of D-40C-s100-0 was 1.15, 1.13, 1.05 times higher than D-40C-s300-0, D-40C-s300-1, D-40C-s300-2, respectively. The maximum strength of coupling beams with mitigated rebar details increases as the volume fraction of steel fiber increases. Although steel fiber 2% reinforced specimen(D-40C-s300-2) did not satisfy the amount of transverse reinforcement required for seismic design of coupling beam, the overall performance including to maximum strength, ductility and energy dissipation capacity was similar to the control specimen(D-40C-s100-0). As a result, the use of steel fiber with 2% reinforcement can partially replace the transverse reinforcement in diagonally reinforced concrete coupling beam.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.41 no.5
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• pp.502-507
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• 1992

To improve temperature stability, 0.05Pb(SnS11/2TSbS11/2T)OS13T-0.35PbTiOS13T-0.60PbZrOS13T+0.4[wt%]MnO S12T piezoelectic ceramics were manufactured with the addition of CrS12TOS13T by Hot Press method. And the SAW delay line was fabricated and effects of CrS12TOS13T to the propagation characteristics of SAW was investigated, and the SAW filter was fabricated on C4 specimen added by 0.2[wt%] CrS12TOS13T whose propagation characteriatics of surface acoustic wave were the bast and its frequency characteristics was investigated. Electromechancal coupling factor(kS1sTS02T) was 3.11[%] and its temperature coefficient of the center frequency(CS1foT)was -21.27[ppm/$^{\circ}C$] in C4. The 31[MHz]]SAW IF filter of C4 scarcely had diffraction phenomena and therefore it was proper.

• Applied Chemistry for Engineering
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• v.7 no.6
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• pp.1096-1104
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• 1996

In order to synthesize a new antibacterial and antitumor agents, we have prepared new analogues pteroic acid(13a, 13b), which means C-9 position of pteroic acid has been replaced by norfloxacin(8) or ciprofloxacin(9) and amino group of C-2 position by $CH_3$. These derivatives were synthesized coupling at N-4 piperazine of norfloxacin and ciprofloxacin with 2-amino-3-cyano-5-chloromethylpyrazine(20) provided 1-alkyl(ethyl, cyclopropyl)-6-fluoro-1,4-dihydro-4-oxo-7-[[4-N-(2-amino-3-cyanopyrazin-5-yl)methyl]piperazin-1-yl]-3-quinoline-carboxylic acid(12a, 12b). It was then cyclized with acetamidine. HCI to obtain new analogues of C-2 desaminomethylpteroic acid(13a, 13b) in yield of 76.2% and 82.8 % respectively. These compounds were tested in vitro on antibacterial activity against Gram-positive and Gram-negative bacteria including Pseudomonas aeruginosa ATCC9027. In general, these synthesized compounds(13a, 13b) showed less potent activities than those of norfloxacin.

• Journal of the Korean Society of Industry Convergence
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• v.7 no.1
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• pp.107-113
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• 2004

Several transition metal complexes, [$M(L)X_2$](M=Pd(II), Ni(II); X=CI, Br) are prepared with aminophosphine ligands such as 1,2-bis{(diphenylphosphino)amino}ethane{$Ph_2PNHCH_2CH_2NHPPh_2$}($L_1$), 1,2-bis{(diphenylphosphino)amino}propane{$Ph_2PNHCH(CH_3)CH_2NHPPh_2$}($L_2$), trans-1,2-bis{(diphenylphosphino)amino}cyclohexane{$Ph_2PNHC_6H_{10}NHPPh_2$}($L_3$) and 1,2-bis{(diphenylphosphino)amino}benzene{$Ph_2PNHC_6H_4NHPPh_2$}($L_4$). The properties of these complexes are characterized by optical spectroscopic methods including UV/vis spectroscopy, CD, IR, $^1H$- and $^{31}P-NMR$ together with conductometer and elemental analysis. All complexes are stable under atmospheric environment. Catalytic reactivity for C-C coupling between [$M(L)X_2$] and Grignard reagents(RMgX; R=phenyl, propyl, buthyl) by thermolysis were investigated utilizing GC/mass, $^1H$- and $^{13}C-NMR$. When mol scale is 1:20 at [$Pd(L)Cl_2$] and Grignard reagents, the high catalytic activity for C-C coupling is apparent. The [$M(L)X_2$](X=Cl, Br) complexes which have strong bond at M-P exhibit high yields for C-C coupling reactions. When the central metal ion is Pd(II), the high catalytic activity for C-C coupling is apparent. The complex coordinated with Br shows higher catalytic activity for C-C coupling reactions compared to Cl.

• Korean Journal of Materials Research
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• v.13 no.6
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• pp.369-373
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• 2003

Magneto-optic Kerr Effect(MOKE), AFM and magnetoresistance measurements have been carried out on as-deposited and annealed Magnetic Tunnel Junctions(MTJs) with junction sizes 180, 250, 320 and 380 $\mu\textrm{m}$ in order to investigate the correlation among interlayer exchange coupling, surface roughness and junction size. Relatively irregular variations of coercivity $H_{c}$ (∼17.5 Oe) and interlayer exchange coupling $H_{E}$ (∼17.5 Oe) are observed over the junction in as-deposited sample prepared by DC magnetron sputtering. After annealing at $200^{\circ}C$, $H_{c}$ decreases to 15 Oe, while $H_{ E}$ increases to 20 Oe with smooth local variation. $H_{E}$ shows very good correlation with surface roughness across the junction in agreement with Neel's orange peel coupling. The increasing slope per $\mu\textrm{m}$ of normalized $H_{c}$ and $H_{E}$ are same near junction edge along free-layer direction irrespective of junction size, giving relatively uniform $H_{c}$ and $H_{ E}$ for wider junction size. Thickness profiles of the junctions measured with $\alpha$-step show increasingly flat top surface for larger junctions, indicating better uniformity for large. junctions in agreement with the normalized$ H_{c}$ and H$/_{E}$ curves. TMR ratios also increase with increasing junction size, indicating improvement for larger uniform junctions.