• Title/Summary/Keyword: ${\gamma}-ray$

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Selection and Characterization of S-Aminoethyl-L-Cysteine Resistant Plants from Gamma-ray Irradiated Embryogenic Callus in Sweet potato

  • Lee In-Sok;Kim Dong-Sub;Hong Chang-Pyo;Kang Si-Yong;Song Hi-Sup;Lee Sang-Jae;Lim Yong-Pyo;Lee Young-Il
    • Journal of Plant Biotechnology
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    • v.5 no.4
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    • pp.233-238
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    • 2003
  • Sweet potato cells derived from Yulmi were isolated from embryogenic callus and irradiated with 50 Gy dose. Resistant cells were selected on a MS medium containing 1.0 mM S-aminoethyl-L-cysteine (AEC). This level of AEC approximately inhibits non-selected wild type cells. The callus resistant to this analog of lysine was subcultured for 30 days in absence of AEC to proliferate. The three resistant calli (AR-1, AR-2 and AR-3) with better growth were divvied into 0.5~1mm diameter and placed on MS medium with 0, 0.4, 0.6, 0.8 and 1.0 mM AEC. There are considerable growth difference between control callus and AEC resistant callus on the AEC-medium. The selected calli were placed on the hormone-free medium for regeneration. Three plantlets, five plantlets and six plantlets were recovered from AR-1, AR-2 and AR-3 calli, respectively. Each two regenerants in AR-1, AR-2 and AR3 were randomly selected for RAPD and SDS PAGE analysis. RAPD polymorph isms between Yulmi and AEC resistant plant from irradiated calli were detected in several Wako primers. Also, it was identified that two AEC resistant plants had higher protein than the original variety Yulmi.

Comparison of Air Kerma and Absorbed Dose to Water Based Protocols for High Energy Photon Beams: Theoretical and Experimental Study

  • Shin, Dong-Oh;Kim, Seong-Hoon;Seo, Won-Seop;Park, Sung-Yong;Park, Jin-Ho;Kang, Jin-Oh;Hong, Seong-Eon;Ahn, Hee-Kyung
    • Proceedings of the Korean Society of Medical Physics Conference
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    • 2002.09a
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    • pp.241-243
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    • 2002
  • New types of protocols have been recently in development, all based on an absorbed dose-to-water with the aim of improving the accuracy of measurements of absorbed dose to water. IAEA TRS-277, the air-kerma standard-based present protocol, and IAEA TRS-398 and AAPM TG-51, the absorbed dose-to-water standard-based new one, were studied and compared theoretically and experimentally for photon beams of 6, 10, and 15 MV. NE 2571 and 3 Farmer types of ionization chambers in widely commercial use were used to determine an absorbed dose to water at the reference depth in water. Two different kinds of calibration factors were given respectively for every chamber calibrated in $\^$60/CO gamma ray beams from a Korean Secondary Standard Dosimetry Laboratory (KFDA). This work shows that there is around 1 % of difference of absorbed doses measured between two different types of calibration systems owing to different physical parameters and reference conditions used. We hope this work to help form the basis on development of new type of protocol in Korea.

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A Convenient System for Film Dosimetry Using NIH-image Software

  • Kurooka, Masahiko;Koyama, Syuji;Obata, Yasunori;Homma, Mitsuhiko;Imai, Kuniharu;Tabushi, Katsuyoshi
    • Proceedings of the Korean Society of Medical Physics Conference
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    • 2002.09a
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    • pp.260-262
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    • 2002
  • An accurate measurement of dose distribution is indispensable to perform radiation therapy planning. A measurement technique using a radiographic film, which is called a film dosimetry, is widely used because it is easy to obtain a dose distribution with a good special resolution. In this study, we tried to develop an analyzing system for the film dosimetry using usual office automation equipments such as a personal computer and an image scanner. A film was sandwiched between two solid water phantom blocks (30 ${\times}$ 30 ${\times}$ 15cm). The film was exposed with Cobalt-60 ${\gamma}$-ray whose beam axis was parallel to the film surface. The density distribution on the exposed film was stored in a personal computer through an image scanner (8bits) and the film density was shown as the digital value with NIH-image software. Isodose curves were obtained from the relationship between the digital value and the absorbed dose calculated from percentage depth dose and absorbed dose at the reference point. The isodose curves were also obtained using an Isodose plotter, for reference. The measurements were carried out for 31cGy (exposure time: 120seconds) and 80cGy (exposure time: 300seconds) at the reference point. While the isodose curves obtained with our system were drawn up to 60% dose range for the case of 80cGy, the isodose curves could be drawn up to 80% dose range for the case of 31cGy. Furthermore, the isodose curves almost agreed with that obtained with the isodose plotter in low dose range. However, further improvement of our system is necessary in high dose range.

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A Study on the Neutron Activation Analysis of Noble Metals in the Ancient Coin (고전(古錢)내 귀금속 원소의 중성자 방사화 분석에 관한 연구)

  • Kwon Soo Chun;Chul Lee;Myung-Zoon Czae;Jong Du Lee;Koo Soon Chung
    • Journal of the Korean Chemical Society
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    • v.37 no.11
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    • pp.961-966
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    • 1993
  • The determination of noble metals such as Ir, Au and Ag in the ancient coins has been studied. For the measurement of the activity of $^{192}Ir,\;^{198}Au\;and\;^{110m}Ag$, radiochemical separations including solvent extraction and ion-exchange chromatography were applied to reduce the interference of high energy ${\gamma}$-ray emitted from various radionuclides with long half-life. As a results, $10^{-11}$ g/g level of Ir could be detected and it was found that the three kinds of the detection limits, i.e., critical, detection, quantitative limit, calculated by the method proposed by Currie, were enhanced. Prior to the re-irradiation with neutron, inactive carrier was added in order to determine the recovery yield of Ir in the radiochemical separation. The average recovery yields of Ir, Au and Ag in the 5 coins were 65.3%, 98.5%, 99.5%, respectively.

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Genetic improvement of potato plants

  • Suharsono, Sony
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.12-12
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    • 2017
  • Genetic improvement in potato can be carried out through several approaches, as sexual crosses, somatic hybridization, mutation and genetic engineering. Although the approach is different, but the goal is the same, to get a superior cultivar. Mutation and genetic engineering are very interesting methods for genetic improvement of potato plants. Mutation by gamma-ray irradiation have been performed to get some new potato cultivars which are more resistant to disease and have higher productivity. We have carried out a mutation of some potato cultivars and obtained some excellent clones to be potentially released as new superior cultivars. By the mutation method, we have released one potato cultivar for the French fries industry, and we registered one cultivar of potato for chips, and two cultivar for vegetable potatoes. Actually we are doing multi-location trial for three clones to be released as new cultivars. Through genetic engineering, several genes have been introduced into the potato plant, and we obtained several clones of transgenic potato plants. Transgenic potato plants containing FBPase gene encoding for fructose bisphosphatase, have a higher rate of photosynthesis and higher tuber productivity than non-transgenic plants. This result suggests that FBPase plays an important role in increasing the rate of photosynthesis and potato tuber productivity. Some transgenic potatoes containing the Hd3a gene are currently being evaluated for their productivity. Over expression of the Hd3a gene is expected to increase tuber productivity and induce flowering in potatoes. Transgenic potato plants containing MmPMA gene encoding for plasma membrane ATPse are more tolerant to low pH than non-transgenic plants, indicating that plasma membrane ATPase plays an important role in the potato plant tolerance to low pH stress. Transgenic potato plants containing c-lysozyme genes, are highly tolerant of bacterial wilt diseases caused by Ralstonia solanacearum and bacterial soft rot disease caused by Pectobacterium carotovorum. Expression of c-lyzozyme gene plays an important role in increasing the resistance of potato plants to bacterial diseases.

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Study on the Wear Resistant Characteristics of Medium Carbon Alloy Steel Plasma-Nitrided (플라즈마 질화처리된 중탄소합금강의 내마모특성에 관한 연구)

  • Cho, H.S.;Roh, Y.S.;Shin, H.K.;Lee, S.Y.
    • Journal of the Korean Society for Heat Treatment
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    • v.5 no.4
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    • pp.215-223
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    • 1992
  • This study has been performed to investigate into some effects of temperature, gas mixing ratio and time on the optical microstructure, hardness and wear characteristics of medium carbon alloy steel treated by plasma nitriding. The results obtained from the experiment are summarized as follows: (1) Optical micrographs of AISI 4140 steel plasma-nitrided by the double stage technique have revealed that the nitrided layer is composed of the compound layer and the diffusion layer. The variation in temperature at the first stage gives effects, on the formation of compound layer and the growth rate is shown to be relatively fast at $460^{\circ}C$. (2) The thickness of compound layer has been found to increase with increasing nitrogen percentage in the gas mixture and the holding time. It is therefore recommended that a shorter holding time and a lower nitrogen percentage are more effective to produce a tougher compound layer and a diffusion layer only. (3) X-ray diffraction analysis for AISI 4140 steel has shown that the compound layer consist of ${\gamma}^{\prime}-Fe_4N$ and ${\alpha}-Fe$ and that tough compound layer diffustion layer only can be obtained by the double stage plasmanitriding process. (4) There is also a tendency that the total hardened layer depth increases with increasing temperature, time and nitrogen percentage in the first stage during the double stage plasma nitriding. (5) The wear resistance of plasma nitrided specimens has been found thobe considerably increased compared to the untreated specimens and the amount of increment has appeared to increase further with increasing nitriding temperature, holding time and notrogen percentage of gas mixture in the first stage treatment.

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Distribution of Heat Production for the Utilization of Geothermal Resources in Korea. (지열 자원 활용을 위한 국내 열생산율 분포)

  • Kim, Jong-Chan;Lee, Young-Min;Hwang, Se-Ho;Koo, Min-Ho
    • 한국신재생에너지학회:학술대회논문집
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    • 2007.11a
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    • pp.497-500
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    • 2007
  • 지열류량은 지열자원 개발 시 지열 이상대를 찾는데 있어서 중요한 자료로 사용된다. 우리나라의 지체구조별 지열류량 평균은 경기육괴 66 $mW/m^2$, 옥천습곡대 65 $mW/m^2$, 영남육괴 60 $mW/m^2$, 경상분지 72 $mW/m^2$, 연일분지 75 $mW/m^2$이다. 이러한 대륙 내의 지표 지열류량은 상부 지각내의 방사성 동위원소 붕괴에 의한 열생산율(${\sim}40%$)과 하부지각 및 상부맨틀에서부터 전도되어 올라온 지열류량(${\sim}60%$)으로 설명할 수 있다. 따라서 지열류량의 결정에 있어서 열생산율의 정보는 중요한 부분을 차지한다. 열생산율은 지각 내에 존재하는 주요 방사성 동위 원소인 U, Th, K의 붕괴열에 의한 것이며, 열생산율의 측정은 gamma-ray log 자료를 이용하는 방법과 화학분석을 통한 방법이 있다. 이 연구에서는 두 가지 방법을 이용하여 총 123개(화강암 86개, 편마암 37개) 지점에서의 열생산율을 산출하였다. 화강암의 열생산율 평균은 2.15 ${\mu}W/m^3$이며, 편마암의 열생산율 평균은 2.22 ${\mu}W/m^3$로 나타났다. 지체구조별 열생산율의 평균은 경기육괴 2.52 ${\mu}W/m^3$, 옥천습곡대 2.16 ${\mu}W/m^3$, 영남육괴 2.35 ${\mu}W/m^3$, 경상분지 2.01 ${\mu}W/m^3$로 나타났다. 지체 구조별 열생산율과 지열류량의 상관성 분석에서 우리나라의 경우 지열류량이 높은 지역에서 열생산율이 낮은 경향을 보인다. 따라서 열생산율이 지표 지열류량을 결정하는데 있어서 가장 중요한 요소가 아님을 확인할 수 있다.

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RTD Analysis using Radioisotope Tracer on the Water Flow Characteristics in a Flocculator of Wastewater Treatment Facility (방사성동위원소 추적자를 이용한 폐수처리시설의 응집조에서 유입수의 체류시간분포 분석)

  • Kim, Jin-Seop;Jung, Sung-Hee;Kim, Jong-Bum
    • Journal of the Korean Society for Nondestructive Testing
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    • v.26 no.1
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    • pp.1-6
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    • 2006
  • Using In-113m emitting gamma ray of 0.392MeV at radioisotope tracer the RTD (residence time distribution) of water in the flocculator of wastewater treatment facility was measured. The result was analyzed mathematically using K-RTD program constructed on the basis of CFSTR (constant flow stirred tank reactor) model. The mean residence time and the tank number are the main parameters which describe the flow behavior of the system. Those parameters were obtained in the fitting profess of the simulated curves to the experimental results. It was suggested to construct a modified numerical model to describe the bypass flow which was observed in the experiment.

The Magnetic Characteristics and Microstructure of Mn-A1 System Alloys(1st Report) -Focused on the Mn-A1 Alloys- (Mn-Al계 합금의 열처리에 따른 미세조직 변화와 지기적 특성(제1보) -Mn-Al-Cu 합금을 중심으로-)

  • Pang, Man-Gyu;Yang, Hyun-Soo;Kwak, Chang-Sup
    • Journal of the Korean Society for Precision Engineering
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    • v.5 no.4
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    • pp.48-58
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    • 1988
  • This study was undertaken to observe the formation behavior of ferro- magnetic phase in Mn-Al-Cu Alloys. The alloy selected for this investigation was 70% Mn-29% Al-1% Cu. This pre-allyed pig was prepared to the cylinderical castings using an Induction furnace after homogenizing at $1100^{\circ}C$ for 2hr, the specimens were cooled by cooling methods. Subwequent isothermal heat treatments were followed at $550^{\circ}C$ for various periods of time at predetermined(1-1000min). The formation behavior of ferromagnetic phase was investigated by measurements of magnetic properties of the specimens at each stage of heat treatment, and optical microscopic esamination and X-Ray diffraction analyses were also employed. By this basic experimental results, the conclusions are as follows 1) In order to obtain much amount of ferromagnetic phase, the optimum average cooling rate was about 7.35-$16.4^{\circ}C$/sec($1100^{\circ}C$-$600^{\circ}C$). 2) We verified the decomposition of {\tau} phase to {\beta} -Mn and {\gamma} , as the specimens were homogenized at $1100^{\circ}C$ for 12hr, then heat-treased at $550^{\circ}C$ for 1-1000min. 3) A condition of optimum heat treatments in Mn-Al-Cu permanent mag-netic alloys showed that after homogenizing at $1100^{\circ}C$ for 2hr, the speciments were cooled in air or furnace(A) and subsequent heat treatments at $550^{\circ}C$ for 1-30min. The maximum magnetic properties were measured as follows: Air cooling; Br=1200(Gause), bHc=100(oe), (BH)max=0.07(MGOe) Furnace cooling(A);Br=950(Gauss), bhe=80(Oe), (BH)max=0.05(MGOe)

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Wide-Field Imaging Telescope-0(WIT0): A New Wide-Field 0.25 m Telescope at McDonald Observatory

  • Lee, Sang-Yun;Im, Myungshin;Pak, Soojong;Ji, Tae-Geun;Lee, Hye-In;Hwang, Seong Yong;Marshall, Jennifer;Prochaska, Travis;Gibson, Coyne A.
    • The Bulletin of The Korean Astronomical Society
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    • v.42 no.1
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    • pp.34.2-34.2
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    • 2017
  • A small wide-field imaging telescope is a powerful instrument to survey the Universe: wide-field image can monitor the variability of many sources at a time, e.g. young stellar objects and active galactic nuclei, and it can be an effective way to locate transient sources without precise positional information such as gravitational wave sources or some gamma-ray bursts. In February 2017, we installed a 0.25 m f/3.6 telescope on the McDonald 0.8 m telescope as a piggyback system. With a $4k{\times}4k$ CCD camera, the telescope has a $2.35{\times}2.35deg$ field-of-view. Currently, it is equipped with Johnson UBVRI filters and 3 narrow-band filters: $H{\alpha}$, OIII and SII. We will present the installation process, and the telescope performance such as detection limit and image quality based on the data from commissioning observations. We will also discuss possible scientific projects with this system.

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