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Selection and Characterization of S-Aminoethyl-L-Cysteine Resistant Plants from Gamma-ray Irradiated Embryogenic Callus in Sweet potato  

Lee In-Sok (Korea Atomic Energy Research Institute)
Kim Dong-Sub (Korea Atomic Energy Research Institute)
Hong Chang-Pyo (Chungnam National Univ. Department of Horticulture)
Kang Si-Yong (Korea Atomic Energy Research Institute)
Song Hi-Sup (Korea Atomic Energy Research Institute)
Lee Sang-Jae (Korea Atomic Energy Research Institute)
Lim Yong-Pyo (Korea Atomic Energy Research Institute)
Lee Young-Il (Korea Atomic Energy Research Institute)
Publication Information
Journal of Plant Biotechnology / v.5, no.4, 2003 , pp. 233-238 More about this Journal
Abstract
Sweet potato cells derived from Yulmi were isolated from embryogenic callus and irradiated with 50 Gy dose. Resistant cells were selected on a MS medium containing 1.0 mM S-aminoethyl-L-cysteine (AEC). This level of AEC approximately inhibits non-selected wild type cells. The callus resistant to this analog of lysine was subcultured for 30 days in absence of AEC to proliferate. The three resistant calli (AR-1, AR-2 and AR-3) with better growth were divvied into 0.5~1mm diameter and placed on MS medium with 0, 0.4, 0.6, 0.8 and 1.0 mM AEC. There are considerable growth difference between control callus and AEC resistant callus on the AEC-medium. The selected calli were placed on the hormone-free medium for regeneration. Three plantlets, five plantlets and six plantlets were recovered from AR-1, AR-2 and AR-3 calli, respectively. Each two regenerants in AR-1, AR-2 and AR3 were randomly selected for RAPD and SDS PAGE analysis. RAPD polymorph isms between Yulmi and AEC resistant plant from irradiated calli were detected in several Wako primers. Also, it was identified that two AEC resistant plants had higher protein than the original variety Yulmi.
Keywords
Embryogenic callus; Induced variation; RAPD; Saminoethyl-L-cysteine; SDS-PAGE; Sweet potato;
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