• The Journal of Korean Physical Therapy
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• 제16권3호
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• pp.22-31
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• 2004

The purpose of this study was to demonstrate the effects of silver spike point (SSP) low frequency electrical stimulation on plasma ${\beta}-endorphin$ activities measured by radio- immunoassay from normal volunteer and the effects of ${\beta}-endorphin$ on 5-hydroxytryptamine (5-HT, serotonin)-induced contraction investigated by isometric tension methode in rats. The current of 3 Hz continue type, but not 100 Hz continue type, of SSP low frequency electrical stimulation significantly increased in plasma ${\beta}-endorphin$ from normal volunteer. The endothelial cell-dependent 5-HT-induced contractions were inhibited by ${\beta}-endorphin$ $1{\mu}M$. These results suggest that the ${\beta}-endorphin$ regulates nociceptive-like substance, such as 5-HT, in part and that the SSP low frequency electrical stimulation, specifically current of low frequency of 3 Hz continue type, significantly increases plasma ${\beta}-endorphin$ from normal volunteer.

• 한국동물학회지
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• 제33권2호
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• pp.152-157
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• 1990

면역조직화학적 방법을 이용하여 생쥐 난소에서 $\beta$-endorphin이 생성되는 부위를 조사하기 위하여 본 실험을 행하였다. 성숙한 생쥐를 4% neutral buffered paraformaldehyde로 관류고정하였으며 난소를 적출한 후 cryostat로 절편을 만들어 avidin-biotin complex(ABC)를 이용하여 면역색을 하였다. $\beta$-endorphin에 대한 항체반응은 주로 황체에서 일어났으며, theac interna와 theca externa에서는 반응이 나타나지 않았다. 황체에서의 염색 양상은 퇴화가 많이 진행된 황체세포에서 보다 강한 연색반응을 관찰할 수 있었다. 때로 황체세포에서 유래된 것으로 보이는 간질세포에서도 양성반응을 관찰할 수 있었다. 이외에도 large antral follicle의 여포강에 인접한 과립세포에서 약한 반응을 보여주었으나, 제 1차 여포에서는 양성반응이 나타나지 않았다.

• 대한약리학회지
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• 제20권2호
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• pp.35-47
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• 1984

실험적으로 L:D, 12:12또는 D:D, 12:12에 적응시킨 흰쥐의 간뇌에서 beta-endorphin함량의 일중변동과 이에 대한 수종 중추성 약물의 효과를 검토하고 beta-endorphin함량의 생리 및 약리학적 의의를 추구코저 $^3H-morphine$ binding을 측정하여 다음과 같은 성적을 얻었다. 1) L:D, 12:12 cycle에 적응시킨 대조군에서 뇌내 beta-endorphin함량은 06 : 00시 에 최고에 달하고 18 : 00시에 최저인 대단히 유의한 일중변동을 일으켰고 24시간 평균 beta-endorphin함량은 $46.7{\pm}3.6$ fmole/mg protein 이었다. 2) D:D, 12:12에 적응시친 표본에서 beta-endorphin 함량은 대조군에서와는 달리 14 : 00시에 최고에 이르고 02 : 00시에 최저에 이르는 일중변동을 보였으며 24시간 평균 beta-endorphin함량도 대조군에 비하여 현저히 감소되었다. 3) 뇌내 beta-endorphin함량 일중별동은 reserpine. pargyline, imipramine, amphetamine. 또는 chlorpromazine처리로써 변동되었다. 4) 대조군에서 $^3H-morphine$ binding은 22 : 00시에 최고에 달하는 일중변동을 보였으며, 지속적인 암적응군에서의 binding은 02 : 00최고, 그리고 14 : 00시에 최하에 이르는 일중변동을 보였다. 5) 대조군과 지속적인 암적응군에서 함량과 maximum $^3H-morphine$ binding간에는 유의한 역상관관계가 있었다. 이상의 실험성적으로 흰쥐 뇌내 beta-endorphin함량과 수용체는 유의란 일중변동을 일으키고 opiate receptor binding의 일중변동은 beta-·endorphin함량변동에 따른 receptor regulation에 의함을 시사하며 또한 지속적인 암적응을 포함하여 수종 중추에 작용하는 약물들이 endorphin함량 일중변동을 변동시킬 수 있음을 보여 준다.

• The Journal of Korean Physical Therapy
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• 제5권1호
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• pp.39-46
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• 1993

The purpose of this study was to examine the effect and $\beta-endorphin$ level as conventional transcutaneous electrical nerve stimulation (TENS) application on acupuncture paints. Twelve healthy adult male volunteers were participated in this study. The subjects were assigned to TENS group (n=6) and naloxone group (n=6). The LI 3 and M 10 meridian points of dominant arm were stimulated comfortably with 100 pps, $75{\mu}s$ conventional TENS for 30 minutes. Experimental pain threshold measurement and plasma $\beta-endorphin$ level were detected before and after conventional TENS application. Experimental pain threshold increased significantly (p<.01) but plasma $\beta-endorphin$ level was not change in TENS group. Experimental pain threshold increased significantly (p<.01) but plasma $\beta-endorphin$ level was not change in naloxone group. In this study, the conventional TENS induced analgesic effect, and plama $\beta-endorphin$ level was not increase concomitantly with analgesia. These results suggest that the $\beta-endorphin$ did not involved in conventional TENS analgesia.

• Clinical and Experimental Reproductive Medicine
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• 제17권2호
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• pp.159-165
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• 1990

It has been reported that endogenous opioid peptides play a role in the control of the reproductive function. The goal of this study was to evaluate changs in the serum levels of ${\beta}$-endorphin during hyperstimulated menstrual cycle and their relationship to serum prolactin levels. Serum ${\beta}$-endorphin and prolactin levels were measured during menstrual cycles of 10 normal cylic women hyperstimulated by human menopausal gonadotropin (HMG) and of 10 women by clomiphene/HMG among in vitro fertilization candidates. The results were summarized as follows. 1. In clomiphene/HMG hyperstimulated menstrual cycle the mean serum ${\beta}$-endorphin level insignificantly on 2 day before aspiration of oocyte compared to basal level and reached maximum level on 1 day after aspiration. 2. There was a significant peak of the mean serum ${\beta}$-endorphin level on 1 day before aspiration in HMG hyperstimulated menstrual cycle. 3. On the same day from aspiration, there was no significant differences in the mean serum ${\beta}$-endorphin levels between HMG and clomiphene/HMG hyperstimulated cycles. 4. No significant correlation was noted between serum ${\beta}$-endorphin levels and prolactin levels.

• International Journal of Oral Biology
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• 제34권3호
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• pp.137-142
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• 2009

Whole-body $\gamma$-irradiation(WBI), which produces an oxidative stress, is reported to attenuate the acute antinociceptive action of morphine (a $\mu$-opioid receptor agonist), but not DPLPE (a $\delta$-opioid receptor agonist), in mice. Recently, we also reported that antinociceptive effect of morphine, but not $\beta$-endorphin (a novel $\varepsilon$-opioid receptor agonist), was attenuated by oxidative stress. These findings prompted us to investigate the effect of WBI on the antinociception of morphine and $\beta$-endorphin in mice. Mice were exposed to WBI (5 Gy) from a $^{60}Co$ gamma-source and tested 2 hours later for antinociception produced by intracerebroventricular administration of morphine or $\beta$-endorphin using the hot water tail-immersion and the writhing tests. WBI significantly attenuated the antinociception produced by morphine only in the hot water tail-immersion test, whereas the antinociception of $\beta$-endorphin was significantly potentiated by WBI in both tests. These results demonstrate a differential sensitivity of $\mu$- and $\varepsilon$-opioid receptors to WBI, and support the hypothesis that morphine and $\beta$-endorphin administered supraspinally produce antinociception by different neuronal mechanisms.

• 대한약리학회지
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• 제21권2호
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• pp.128-141
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• 1985

실험적으로 명암에 적응시킨 백서의 뇌내 specific opiate binding과 ${\beta}-endorphin$ 함량 일중변동에 미치는 지속적인 암적응, phenobarbital 장기처리의 영향을 관찰하고 opiate recptor binding과 ${\beta}-endorphin$ 함량 양자간의 관계를 추구하여 다음과 같은 성적을 얻었다. 1. L : D, 12 : 12 주기에 적응시킨 대조군에서 maximum $(^3H)$-morphine binding과 뇌내 ${\beta}-endorphin$ 함량은 각각 22시 및 06시에 최고에 달하는 매우 특이한 일중변동을 보였고 24시간 평균 $(^3H)$-morphine binding 및 뇌내 ${\beta}-endorphin$ 함량은 각각 0.45+0.03 pmol/mg protein과 46.7+3.6 fmol/mg protein이었다. 2. D : D, 12 : 12 주기에 적응시킨 표본에서 maximum $(^3H)$-morphine binding과 뇌내 ${\beta}-endorphin$ 함량은 대조군에서와는 달리 02시 및 14시에 최고에 달하는 유의한 일증변동을 보였고, 24시간 평균 maximum $(^3H)$-morphine binding치는 0.36+0.03 pmol/mg protein으로 대조군에 비하여 유의하게 감소를 하였으며, 24시간 정균 뇌내 ${\beta}-endorphin$치는 35.9+3.1 fmol/mg protein으로 대조군에 비하여 유의한 감소를 보였다. 3. Phenobarbital처리 표본에서 maximum $(^3H)$-morphine binding과 뇌내 ${\beta}-endorphin$ 함량은 각각 02시 및 14시에 최고 그리고 14시 및 02시에 최저에 달하는 대조군과는 다른 일중변동을 보였고, 24시간 평균 maximum $(^3H)$-morphine binding치는 0.33+0.03 pmol/mg protein으로 대조군에 비하여 현저한 감소를 보였다. 4. 전 실험군에서 opiate receptor binding의 Kd치는 변동하지 않았다. 5. 전 실험군에서 maximum $(^3H)$-morphine binding은 ${\beta}-endorphin$ 함량과 유의한 역상관관계가 있었다. 이상의 실험성적은 phenobarbital이 뇌내 ${\beta}-endorphin$ 함량과 opiate수용체의 숫적변동을 일으켜 morphine의 약리적 작용을 변동시킬 수 있으며, 이와 더불어 opiate 수용체와 ${\beta}-endorphin$ 함량 일중변동을 변화시킬 수 있음을 보여준다.

• The Journal of Korean Physical Therapy
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• 제6권1호
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• pp.5-15
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• 1994

The purpose of this study was to examine the effect of electroanalgeia and $\beta-endorphin$ action by acupuncture-like (Lof/Hil) transcutaneous electrical nerve stimulation (TENS) applied to acupuncture points. Twelve healthy adult male aged between 19 ann 25 were randomly assigned to TENS group (n=6) and naloxone group (n=6). Subjects of both groups were strongly stimulated TENS with 4 pps and $200{\mu}s$ for 30 minutes on the LI 3 and LI 10 meridian points of dominant am. Naloxone group was injected naloxone hydrochloride before TENS application. The experimental pain threshold was measured by chronaxie meter CX-2 on the distal end of radius just before and after TENS application. The levels of plasma $\beta-endorphin$ and ACTH. serum cortisol and urinary 17-OHCS were analyzed by radioimmunoassay (RIA) kits before and after TENS application. In TENS group, there was a significant increase of experimental pain threshold (p<0.01), plasma $\beta-endorphin$ level (p<0.05), serum cortisol level (p<0,001) and urinary 17-OHCS levels (p<0.05) after TENS application. The plasma ACTH level was not significantly increased, but it showed an increasing tendency. In naloxone group, although there was a decreasing trend, ACTH and cortisol level did not show a significant change, but $\beta-endorphin$ and 17-OHCS level were significantly decreased (p<0.01). The result of this study stewed that acupuncture-like TENS induced analgesic effect, such that the levels of plasma $\beta-endorphin$, plasma ACTH, serum cortisol and urinary 17-OHCS were concomitantly increased with experimental pain threshold. It is suggested that the analgesic mechanism of the acupuncture-like TENS probably related to endogenous opioid component such as $\beta-endorphin$.

• The Journal of Korean Physical Therapy
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• 제9권1호
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• pp.157-166
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• 1997

The purpose of this study was to determine the effects of laser Irradiation on Human plasma $\beta-endorphin$ levels, by treating with low level helium-neon (He-Ne) and Infrared(lR) laser. The Laser was fixed frequency of 2400Hz by continuous scanning and irradiating time was 8 minutes each point. Blood samples were taken at before, after, after 15min's treatment and Plasma $\beta-endorphin$ was measured by radioimmunoassay. The samples for this study were 6 normal subjects(3male, 3female). The data were analyzed by paired t-test, one-way ANOVA and simple regression. The results of this study were as follows : 1. The human plasma $\beta-endorphin$ levels were noted as significant increase in after-treatment $(22.84{\pm}10.63pg/ml)$ as compared with before-treatment $(16.96{\pm}9.23pg/ml)$ and significant increase in after 15min's $(27.27{\pm}8.81pg/ml)$ as compared with after-treatment (p<0.05). 2. There were no significant changes in plasma g-endorphin levels between male and female. 3. The human plasma $\beta-endorphin$ levels were high associated in between session reliability (p<0.05).

• The Journal of Korean Physical Therapy
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• 제9권1호
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• pp.103-115
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• 1997

The purpose of this study was to examine the amount of $\beta-endorphin$ and pain threshold according to time sequences in applying experimented TENS and MENS(pre treatment, posttreatment, 25 minutes after the treatment). This test was to aim at showing the effects of the experimental $\beta-endorphin$ and pain threshold applied to the randomly selected twenty healthy men in their twenties. The subjects were divided into two group(ten for TENS and the other ten MENS). The results obtained are as follows : 1. There was no significant difference in the $\beta-endorphin$ between TENS and HENS(p>0.05). There was no difference in the pain threshold at pretreatment and posttreatment(p>0.05), but there was some difference at 25 minutes after the treatment(p<0.05). 2. The group of TENS in the experimental $\beta-endorphin$ had the highest level of $14.40{\pm}3.098$ at posttreatment, but the level decreased a little according to time passed. And in the experimental pain threshold, the level went to the highest plateau of $2.92{\pm}0.483$ at 25 minutes after the treatment. 3. The group of MENS in the experimental $\beta-endorphin$ had the highest plateau of $14.20{\pm}3.967$ at posttreatment, but the level decreased a bit according to time passed also. And in the experimental pain threshold, the level went to the highest plateau of $2.49{\pm}0.617$ posttreatment. 4. There were some differences of the experimental $\beta-endorphin$ in TENS group at pretreatment and posttreatment(p<0.05). There were some differences in the experimental pain threshold between pretreatment and posttreatment as well as between pretreatment and 25 minutes after the treatment(p<0.05) MENS did not influence the experimental $\beta-endorphin$ and pain threshold. This experiment showed that TENS increased the levels of the experimental $\beta-endorphin$ at posttreatment and increased the levels of the experimental pain threshold untill 25 minutes after the treatment. Therefore, the time of sustaining pain in TENS group was longer than that of MENS group. Also, MENS showed that it increased each level of the experimental $\beta-endorphin$ and pain threshold, but these levels were not statistically meaningful.