• Title/Summary/Keyword: ${\beta}$-glucosidase

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Growth Inhibition of Clostridium difficile by Fermented Broccoli with Leuconostoc mesenteroides (Leuconostoc mesenteroides를 이용한 브로콜리 발효물에 의한 Clostridium difficile의 생육 제어)

  • Lee, Young-Duck;Moon, Gi-Seong
    • Journal of Food Hygiene and Safety
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    • v.32 no.6
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    • pp.531-535
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    • 2017

  • In this study, Leuconostoc mesenteroides CJNU0041 was isolated from Korean traditional food kimchi and antimicrobial activity of fermented broccoli with the isolate was tested against pathogenic Clostridium difficile. L. mesenteroides CJNU0041 showed higher glucosidase activity than other isolates. As the results of physiological properties such as pH and viable cell count during broccoli fermentation with L. mesenteroides CJNU0041, we confirmed that 48 hours was optimal fermentation time. As the results of metabolite analysis by HPLC, metabolites were changed during the fermentation. Especially, the growth of C. difficile was inhibited by the fermented broccoli. Therefore, L. mesenteroides CJNU0041 might be a candidate for improving the functionality of natural materials by lactic acid fermentation.

  • https://doi.org/10.13103/JFHS.2017.32.6.531 인용 PDF KSCI

Effects of Samiunkyungtang on inflammation and fecal enzymes in ulcerative colitis animal model (궤양성 대장염 동물모델에서 사미연견탕(四味軟堅湯)의 항염증 및 장내세균 효소활성 억제효과)

  • Kim, So-Yeon;Ryu, Bong-Ha;Park, Jae-Woo
    • The Journal of Korean Medicine
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    • v.29 no.3
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    • pp.50-62
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    • 2008

  • Objectives: This study was aimed to find the effect of Samiunkyungtang on inflammation and microflora in an ulcerative colitis animal model. Methods: We established four groups of normal, control, test 1, and test 2 and assigned 6 rats toeach group. The normal group was not treated by any process and fed by normal saline. The control & test groups were provided with 4% dextran sodium sulfate (DSS) treatment for 7 days. Samiunkyungtang extract was orally administered to test groups (test 1=25mg/kg, test 2 100mg/kg) 3 days after DSS treatment for 10 days. After DSS treatment finished, we sacrificed the mice and measured colon length and enzyme activities such as myeloperoxidase (MPO), alkine phosphatase (ALP), ${\beta}$-glucuronidase, ${\beta}$-glucosidase, chondroitinase, and tryptophanase. Results: The colon lengths of test 1 and 2 groups were longer than the control group (p<0.05). Histologically, the crypts and superficial epitheliums of test 1 and 2 groups were regenerated. Goblet cells from all test groups were retrieved. The inflammatory biochemical marker, MPO and ALP activities in all test groups were highly reduced (p<0.01) compared to the control group. The activities of fecal bacterial enzymes in test groups such as ${\beta}$-glucuronidase, ${\beta}$-glucosidase, chondroitinase, and tryptophanase were reduced compared to the control group (p<0.01). Conclusions: As a result of this experiment, Samiunkyungtang is considered to have an inhibitory effect on inflammation and fecal enzyme activity in DSS-induced colitis animal model. Our results indicate that Samiunkyungtang may possess therapeutic effect on the development of DSS-induced colitis.

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Cloning, Characterization of Pichia etchellsii $\beta-Glucosidase$ II and Effect of Media Composition and Feeding Strategy on its Production in a Bioreactor

  • Sethi Benu;Jain Monika;Chowdhary Manish;Soni Yogesh;Bhatia Yukti;Sahai Vikram;Mishra Saroj
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.7 no.1
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    • pp.43-51
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    • 2002

  • The cloning and expression of $\beta-glucosidase$ II, encoded by the gene ${\beta}glu2$, from thermotolerant yeast Pichia etchellsii into Escherichia coli is described. Cloning of the 7.3 kb BamHI/SalI yeast insert containing ${\beta}glu2$ in pUC18, which allowed for reverse orientation of the insert, resulted in better enzyme expression. Transformation of this plasmid into E. coli JM109 resulted in accumulation of the enzyme in periplasmic space. At $50^{\circ}C$, the highest hydrolytic activity of 1686 IU/g protein was obtained on sophorose. Batch and fed-batch techniques were employed for enzyme production in a 14 L bioreactor. Exponential feeding rates were determined from mass balance equations and these were employed to control specific growth rate and in turn maximize cell growth and enzyme production. Media optimization coupled with this strategy resulted in increased enzyme units of 1.2 kU/L at a stabilized growth rate of $0.14\;h^{-l}$. Increased enzyme production in bioreactor was accompanied by formation of inclusion bodies.

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