• Asian-Australasian Journal of Animal Sciences
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• 제32권10호
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• pp.1548-1557
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• 2019

Objective: To investigate the effects of dietary supplementation with different levels and molecular weights of fungal ${\beta}$-glucan on productive performances, health, carcass traits and meat quality in broilers. Methods: Two hundred and ten of one-day-old chicks with equal sex were assigned to seven experimental groups in $2{\times}4$ factorial arrangement. These groups were supplemented with (0, 10, 30, and 60 ppm) of molecular weight 1-3, 1-6 ${\beta}$-glucan (low or high). High molecular weight ${\beta}$-glucan (H: 943 kDa) was obtained from Ophiocordyceps dipterigena BCC 2073, whereas H with ${\gamma}$-Irradiation treatment was performed to achieve low molecular weight ${\beta}$-glucan (L: 8 kDa). Results: There was no statistical significance in productive performances, apparent digestibility and interaction between fixed factors along 42 days of experiment (p>0.05). A higher caecal amylase activity was present in the group that received L, while there was a dramatic decrease in H and the control groups, respectively (p<0.05). The increase of supplemental dose increased caecal amylase activity (p<0.05). Immunomodulatory effects from L was revealed by the marked increase of phagocytic activity, relative weight of thymus and bursa of fabricius (p<0.05). Similarly, the additive dose at 30 ppm provided the same results, whereas the only significant difference with supplementation at 60 ppm was an increase in phagocytic activity (p<0.05). Interestingly, villi height of broilers fed L was higher than other groups (p<0.05). The treatments did not influence haematology, blood chemistry, antibody production level against vaccination, carcass traits and meat quality (p>0.05). Conclusion: The supplementation of L at 30 ppm was suggested to achieve benefits of immune modulation without adverse effects on other parameters.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1999년도 춘계 학술대회지
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• pp.128-129
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• 1999

• Journal of Microbiology and Biotechnology
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• 제2권2호
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• pp.85-91
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• 1992

The intestinal microflora of humans is an extraordinarily complex mixture of microorganisms, the majority of which are anaerobic microorganisms. The distribution of amylolytic microorganisms in the human large intestinal tract was investigated in various individuals of differing ages using anaerobic culture techniques. A large percentage of the amylolytic microorganisms present belonged to the Genus Bifidobacteria. The number of Bifidobacteria increased significantly at two years of age. Adults and children above 2 years old carried about $0.8{\times}10^9-2.0{\times}10^{10}$ colony forming units (CFU/gram) of amylolytic Bifidobacteria. Among these amylolytic Bifidobacteria, Int-57 was chosen for further studies. Between 65% and 85% of the amylase produced was secreted and the remaining amylase was bound to the cell wall facing the outside. Amylase production could be induced by starch in a stable form. When cells were grown on maltose or glucose, amylase production was much lower than on starch and amylase activity disappeared after 24 hours growth on these media. Partially purified enzymes showed optimum activity at a temperature of $50^{\circ}C$ and at an optimum pH of 5.5, respectively. Heat treatment at $70^{\circ}C$ for 30 minutes almost completely inactivated amylase. The hydrolysis products of starch were mainly maltose and maltotriose. Soluble starch, amylose, amylopectin, and $\gamma$-cyclodextrin($\gamma$-CD) were easily hydrolyzed. The rate of hydrolysis of $\alpha$-CD and $\beta$-CD was slower than that of $\gamma$-CD. Carboxymethyl cellulose, $\beta$-1, 3-glucan and inulin were not hydrolyzed.

• 한국작물학회지
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• 제42권4호
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• pp.403-409
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• 1997

취반용 보리 종실 내 (1-3, 1-4)-$\beta$-glucan의 축적기작을 알아보기 위하여 개화 후 일정 간격으로 보리 종실을 채취하여 (1-3, 1-4)-$\beta$-glucan 함량, (1-3, 1-4)-$\beta$-glucanase 활성 및 전분함량과 $\alpha$-amylase 활성을 조사하였다. $\beta$-glucan 함량은 등숙 초기에 매우 낮았으나 개화 후 15~25일 사이에 급속히 증가하였고 개화 후 30일경에는 종실 건물중의 3.5~4%로 증가하였다. (1-3, 1-4)-$\beta$-glucanase 활성은 등숙 초기에 높았으며 등숙이 진전될수록 감소하였다. $\beta$-glucan 함량과 (1-3, 1-4)-$\beta$-glucanase 활성간에는 고도로 유의한 부의 상관관계가 인정되었으나 등숙 종실 중의(1-3, 1-4)-$\beta$-glucanase 활성이 매우 낮은 수준이므로 $\beta$-glucan 축적량에 크게 영향을 미치지 않을 것으로 추정되었다. 전분함량은 등숙이 진전됨에 따라 지속적으로 증가하였으며 개화 후 10~20일에 가장 뚜렷하게 증가하였다. 포도당함량은 등숙이 진전됨에 따라 현저히 감소하였으며, 등숙 초기와 중기에 감소 정도가 매우 컸다. $\alpha$-amylase의 활성도 등숙이 진전됨에 따라 현저히 감소하였으며, 감소 정도는 등숙 초기와 중기에 매우 컸다.

• Journal of Microbiology and Biotechnology
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• 제2권3호
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• pp.189-196
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• 1992

A $\alpha$-l, 4-D-glucan maltohydrolase $(\beta$-amylase), secreted by the mesophilic aerobic bacterium Bacillus polymyxa No.26, was purified and characterized. The enzyme production was increased after a logarithmic phase of bacterial growth and paralleled with the onset of bacterial sporulation. By applying anion exchange chromatography and gel filtration the enzyme was purified 16.7-fold and had a specific activity of 285.7 units/mg. Two enzyme activities were eluted on a column of DEAE-Sephadex chromatography, and they were designated as E-I for a major enzyme peak and E-II for a minor peak. Of them, E-I enzyme peak was further purified by using gel chromatography. The molecular mass of this enzyme was determined to be 64, 000 daltons and consisted of a single subunit, showing an isoelectric point of 8.9. The enzyme was able to attack specifically the $\alpha$-l, 4-glycosidic linkages in soluble starch and caused its complete hydrolysis to maltose and $\beta$-limited dextrin. This amylolytic enzyme displayed a temperature optimum at $45^\circ{C}$ and a pH optimum at 7.0. The amino acid composition of the purified enzyme was quite similar to the other bacterial $\beta$-amylases reported. Surprisingly, the purified enzyme from this aerobe only exhibited hydrolytic activity on soluble starch, not on starch granules. The degradation of from starch by $\beta$-amylase was greatly stimulated by pullulanase addition. These results differentiated from other $\beta$-amylases reported. Based on a previous result that showed the enzyme system involves in effective degradation of raw starch granules, this result strongly suggested that the purified enzyme (E-I) can be a synergistic part of starch granule-digestion and E-II plays a crucial role in digestion of starch granules.

• 한국식품영양과학회지
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• 제11권2호
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• pp.1-6
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• 1982

직접채취(直接採取)한 3종(種)의 벌꿀을 표준으로 하여 시판(市販)꿀의 당조성(糖組成), 효소력(酵素力) 및 화분수(花粉數)를 조사비교(調査比較)한 결과(結果)는 다음과 같다. 1. 시판(市販)꿀의 전당함양(全糖含量)은 표준꿀보다 약간 많았다. 2. 표준꿀의 화분수(花粉數)는 시판(市販)꿀보다 약 5배(倍)나 많았다. 3. 표준꿀은 시판(市販)꿀보다 ${\alpha}-amylase$는 5배(倍), ${\beta}-amylase$는 2배(倍), protease activity는 평균 2배(倍)나 높았다. 4. 이상(以上)의 결과(結果)로 보아 시판(市販를)꿀은 sugar honey이거나 synthetic honey가 일부 들어 있는지 의심스럽다. 그래서 표준꿀에 대한 조사(調査)를 충분(充分)히 해서 판정기준(判定基準)을 설정(設定)하여 불량시판(不良市販)꿀이 없도록 해야겠다.

• Applied Biological Chemistry
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• 제35권4호
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• pp.232-236
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• 1992

생대두를 이용한 발효장류의 개발을 위한 기초조사로서 대두의 처리조건을 찾고저 처리방법 [생콩(A), 수침탈각(B), 건열처리(C) 및 습열처리(D)]을 달리한 대두의 일반성분과 효소활성변화를 검토하였다. 그 결과 일반성분 중 수분함량은 A, D구가 B, C구 보다 $2.0{\sim}3.0%$, 조지방 함량은 A, B구가 C, D구 보다 약 2%, 조단백질 함량은 C, D구가 A, B구 보다 $1.16{\sim}1.74%$, 조섬유 함량은 A구가 B, C, D구 보다 $0.11{\sim}1.41%$ 많았으나, 회분은 B, C, D구가 A구 보다 0.49% 많았다. ${\alpha}-amylase$, ${\beta}-amylase$, protease, lipase 활성도는 생콩(A) 및 수침탈각(B) 구가 전열처리(C) 및 습열처리(D)구 보다 약 $2{\sim}5$배 이상 높았다. Trypsin inhibitor 활성도 크기는 $B(56.7{\sim}119.2%)>A(42.9{\sim}95.6%)>D(32.9{\sim}39.6%)>C(20.8{\sim}38.3%)$구 순이었다.

• 한국식품영양학회지
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• 제9권1호
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• pp.45-51
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• 1996

Korean traditional Sikhye is made from rice and malt, and It's main product Is maltose. However commercial Sikhye differs from traditional Sikhye because it's main component is sucrose. Sikhye industry faces many problems such as contamination of malt with microorganisms, low amylase activity of malt and technical difficulties. There is no commercial Sikhye which is only using rice and malt by these reasons. To produce the traditional Sikhye free from these problems, it is necessary to restrict the microorganisms of malt and to standardize the amylase activity of malt. In addition, the Introduction of effective control and sanitaric process is required. In Sikhye production. if $\beta$-amylase and isoamylase or pullulanase were added, starch could be saccharified 100% as maltose. Accordingly, this method brings us the low cost of Sikhye.

• 한국식품영양학회지
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• 제7권4호
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• pp.259-266
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• 1994

The hyperthermophilic archaebacterium Thermococcus profundus Isolated from a deep-sea hydrothermal vent system, produced several amylolytic enzymes such as extracellular amylase and pullulanase, intracellular a-1,4-91ucosidase in respone to the presence of complex carbohydrates In the growth medium. This strain showed high activities on 0.5% maltose than on complex carbohydrates One of the amylases was partially purified by ammonium sulfate precipitation, DEAE-Toyopearl chromatography. The amylase exhibited maximal activity at pH 5.5 and 80$^{\circ}C$, and was stable in the range of pH 5.5 to 9.5 and up to 80$^{\circ}C$ for 30 min. The enzyme activity was no dependence on Ca2+ and not inhibited by detergents. The amylase hydrolyzed soluble starch, amylose, amylopectin and glycogen to produce maltose and maltotriose with trace amounts of glucose, but not pullulan and ${\alpha}$-, ${\beta}$-, ${\gamma}$-cyclodextrin. Malto-oligosaccharides ranging from maltotetraose to maltoheptaose were hydrolyzed in an endo fashion.

• Journal of Ginseng Research
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• 제6권2호
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• pp.131-137
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• 1982

The effect of red ginseng residue on the several enzyme activities of the koji and alcohol fermentation were investigated. The koji showed maximum values of amylase and cellulase activity when it was prepared by 30% red ginseng residue and 70% wheat bran, and of protease activity when it was prepared by 40% red ginseng residue and 60% wheat bran-${\alpha}$ amylase activity of the koji during its fermentation was increased rapidly until 4 days and there after it was increased slowly, but ${\beta}$-amylase was rapidly increased after 3 days fermentation. During the preparation of the koji, the acidic, neutral protease and cellulase activities showed the maximum value after 3 days fermentation and the alkaline protease showed the maximum value within 4-6 days fermentation. On the otherhand, fermented broth, containing 6%(v/v) alcohol, could be obtained when the substrate was saccharified by the koji, based on 25% red ginseng residue and 75% wheat bran, prior to alcohol fermentation.