• 한국환경농학회지
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• 제34권1호
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• pp.57-63
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• 2015

최근 들어 여주의 혈당개선의 약리학적 효능이 보고됨에 따라 기능성 채소로 각광받고 있다. 주로 여주 과실만이 식용으로 쓰이지만 여주씨 또한 다양한 가공과정을 거칠 경우(건여주 혹은 건조분말, 환, 엑기스 등) 필연적으로 함께 섭취되기도 한다. 본 연구에서는 과실이외의 특히, 연구결과가 미비한 여주씨에 대해 생리활성물질의 구명 및 혈당개선의 효과에 대한 연구를 수행하였다. 다양한 추출물 및 단일 활성성분의 혈당개선 in vitro검증법인 ${\alpha}$-glucosidase에 대한 저해효과를 측정하였고, 활성을 나타내는 추출물 또는 활성성분과의 상관관계를 구명하였다. 구체적으로는 95% 에탄올 추출물 및 비극성 용매 등에 다량 추출되는 생리활성물질인 ${\alpha}$-eleostearic acid는 우수한 저해효과를 나타내었다($IC_{50}=43.4{\mu}g/mL$). 또한 효소저해제의 함량을 HPLC를 통한 정량분석을 수행함으로써 여주씨에 함유된 기능성성분의 혈당개선 식 의약 소재로의 응용가능성을 연구하였다.

• 한국식품위생안전성학회지
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• 제29권1호
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• pp.53-59
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• 2014

$Cr^{3+}$ 및 Se을 첨가하여 수경 재배한 치커리 3종의 에탄올 추출물을 각각 제조하여 추출물별 항산화 활성을 비교 검토하였다. 그 결과 양액만으로 재배한 치커리보다 양액에 Se이나 $Cr^{3+}$을 첨가한 치커리 추출물에서 총 폴리페놀 함량, 총 플라보노이드 함량 및 FRAP 환원능이 증가하였으며, DPPH 라디칼 소거능 및 ABTS 라디칼 소거능도 증가하였다. 특히 Se을 첨가한 치커리보다 $Cr^{3+}$을 첨가한 치커리에서 더 우수한 항산화 활성을 나타내었다. 또한 $Cr^{3+}$을 첨가한 CLER 및 CLE의 경우 ${\alpha}$-glucosidase 저해활성이 있음을 알 수 있었다. 따라서 치커리 추출물의 수경재배 과정에서 양액에 $Cr^{3+}$, Se의 첨가는 치커리의 항산화 활성 및 ${\alpha}$-glucosidase 저해활성 증진에 영향을 주는 것으로 확인되었다.

• 대한영양사협회학술지
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• 제25권4호
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• pp.281-294
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• 2019

The objective of this study was to develop fermented vegetable juices that possess antidiabetic and antioxidant activities. Lactobacillus plantarum MKHA15 (MKHA15) and Leuconostoc mesenteroides MKSR (MKSR) were applied to ferment onion, cabbage, and tomato juices at $37^{\circ}C$ and $30^{\circ}C$ for 72 h, respectively, and their functionality was tested using the 12 h hour-fermented juice by MKHA15, and 48 h hour-fermented juice by MKSR. Inhibition of ${\alpha}$-glucosidase activity was observed in all fermented juices. The onion juice fermented by MKHA15 showed significantly higher ${\alpha}$-glucosidase inhibition activity compared to other juices. All juices showed more than 70% inhibition of ${\alpha}$-amylase activity. The DPPH radical scavenging activity of onion juice fermented by MKSR showed significantly lower activity than cabbage and tomato juices; however, no difference was observed between the types of starter cultures. The SOD-like activity of cabbage juice fermented by MKSR was the highest among the fermented juices. The juices fermented by MKHA15 showed higher reducing power than those by MKSR. Therefore, we believe that cabbage, onion and tomato juice fermented by MKHA15 and MKSR would be useful in probiotic juices, as they possess antidiabetic and antioxidant activities.

• Journal of Applied Biological Chemistry
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• 제61권4호
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• pp.379-382
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• 2018

Newcastle disease virus (NDV) 감염된 baby hamster kidney 세포에서 Syncytium (합포체) 형성은 세포막 표면으로의 수송된 바이러스 당단백질 hemagglutinin-neuramidase에 의해 일어난다. HAU 값은 추출물의 농도가 25과 $3.2{\mu}g/mL$ 사이에서는 현저하게 감소하였으나, $25{\mu}g/mL$ 농도에서는 NDV 감염된 HAD (%)는 광범위한 흡착능의 감소를 나타났으나 바이러스 당단백질의 세포내 생합성은 저해되지 않았다. 그러므로 오배자 추출물은 바이러스 당단백질의 세포막으로의 수송과 함께 합포체 형성을 저해하여 항바이러스 활성을 갖는 것으로 결론된다. 또한 오배자 추출물의 저해활성을 조사한 결과 ${\alpha}-glucosidase$에 대한 추출물의 $IC_{50}$은 $12.5{\mu}g/mL$이었으며, ${\beta}-glucosidase$, ${\alpha}-glucosidase$, ${\beta}-mannosidase$에 대한 오배자 추출물의 $IC_{50}$은 각각 26, 36, $50{\mu}g/mL$로 나타나 ${\beta}-type$ glycosidases 보다 ${\alpha}-type$ glycosidase에 대한 효소활성 저해능이 우수하였다. 따라서 $IC_{50}$ 농도에서는 세포내에서 당단백질 생합성은 저해되지 않으며 당단백질의 수송을 저해하는 것으로 판단되었으며 향후 항바이러스 관련 작용기작의 연구가 필요하다고 사료된다.

• Preventive Nutrition and Food Science
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• 제7권1호
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• pp.62-66
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• 2002

As inhibitors of carbohydrate-digesting enzymes can prevent hyperglycemia that is known to cause many macrovascular complications, they may prove a useful adjunct to hypocaloric diets in patients with type 2 diabetes and obesity. Inhibitory activities of two hundred and fifteen kinds of medicinal herb extracts against $\alpha$-glucosidase (EC 3.2.1.20) and $\alpha$-amylase (EC 3.2.1.1) have been investigated in vitro. Adenophora triphylla, Aneilema keisak, and Morus bombysis significantly suppressed rat intestinal $\alpha$-glucosidase activity iu vitro. Porcine pancreatic amylase was efficiently inhibited by methanol extracts of Epimedium koreanum, Campsis grandiflora and Salvia plebeia. Methanol extract of Epimedium koreanum among the medicinal herbs tested showed the strongest inhibitory activity against porcine pancreatic $\alpha$-amylase with 0.1 mg/ML of $IC_{50}$/. The herb extract also improved glucose tolerance in ICR mice when loaded with 0.9 g soluble starch per kg body weight. Taken together, Epimedium koreanum merits further evaluation as a therapeutic measure.

• 생약학회지
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• 제31권1호
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• pp.101-104
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• 2000

An inhibitory activity against ${\alpha}-glucosidase$ was identified in extract of an edible lichen, Umbilicaria esculenta. The inhibitor was very stable retaining above 95% of its original activity when treated with heat, acid and alkaline conditions, and some hydrolytic enzymes. Partially purified inhibitor showed strong inhibition against disaccharide hydrolytic enzymes of mammalian and mold origin, but weak or no inhibition against polysaccharide hydrolytic enzymes except glucoamylase. The inhibitor suppressed elevation of blood glucose level in rats after oral administration of soluble starch or sucrose.

• 한국식품영양학회지
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• 제13권2호
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• pp.134-138
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• 2000

An inhibitory activity against ${\alpha}$-glucosidase was identified in extracts of medicinal lichens, Parmelia austrosinensis and P. praesorediosa. The extracts retained almost all of their original activities when treated with heat, acid and alkaline conditions, and some hydrolytic enzymes. Partially purified inhibitor showed strong inhibition against disaccharide hydrolytic enzymes of mammalian and mold origin, but weak or no inhibition against polysaccharide hydrolytic enzymes except glucoamylase. The inhibitors from the two Parmelia sp. showed almost same retention time in HPLC. The inhibitor suppressed elevation of blood glucose level in rats after oral administration of soluble starch or sucrose.

• 한국자원식물학회지
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• 제27권1호
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• pp.22-28
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• 2014

꽃치자나무 추출물의 HIV-1 reverse transcriptase, protease 및 alpha-glucosidase에 대한 억제활성실험을 실시하였다. ELOSA 방법으로 실험한 역전사효소 억제활성 실험에서는 꽃치자나무 잎 MeOH 추출물 $100{\mu}g/ml$ 농도에서 13.4%의 약한 억제활성이 관찰되었고, 줄기의 메탄올추출물에서는 32.5%의 높은 HIV-1 protease 억제활성과 줄기의 메탄올 추출물에서는 26.1%의 alpha-glucosidase 억제활성이 관찰되었다. 그리고 HIV-1 복제 억제활성은 MT-4 세포에 대한 HIV-1 유도 세포변성억제를 광학현미경으로 관찰하여 살펴본 결과 잎과 줄기의 모든 추출물에서 HIV-1 바이러스 증식억제에 억제활성이 관찰되지 않았다. In silico 실험결과 주요성분인 crocetin이 81.8%의 높은 역전사 효소활성이 예측되었으며, genipin이 55.39%, geniposidic acid aglycone이 64.5% 역전사효소 활성이 예측되었다. 주로 aglycone의 활성이 높게 예측되었으며 배당체의 경우 활성이 현저하게 저하되는 것으로 나타났다.

• 대한한방내과학회지
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• 제27권4호
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• pp.888-894
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• 2006

Objectives : For the purpose of developing new anti-HIV agents from natural sources, the extracts of Ricinus communis were tested for their inhibitory effects on essential enzymes reverse transcriptase (RT), protease and alpha-glucosidase. Inhibition activity of major compounds of Ricinus communis were predicted from quantitative structure activity relationships (QSAR) in silico. Methods and Results : In the anti-HIV-1 RT using enzyme-linked oligonucleotide sorbent assay (ELOSA) method, water and methanol extracts (100ug/ml) of Ricinus communis showed strong activity of 94.2% and 82.7%, respectively. In the HIV-1 protease and alpha-glucosidase inhibition assay, neither water nor methanol extracts of Ricinus communis inhibited the activity of the enzyme to cleave any substrates as oligopeptides and oligosaccharides. Conclusions : We found that for these samples it is possible that the inhibition of the RT in vitro is due to the secondary metabolites of Ricinus communis such as ricinine and quercetin. It would beof great interest to identify the compounds which are responsible for this inhibition, since all therapeutically useful agents up to date are RT inhibitors.

• Food Science and Biotechnology
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• 제18권2호
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• pp.541-545
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• 2009

Water and ethanol extracts were prepared from the haesongi mushroom (Hypsizigus marmoreus) to measure functional components. The ability of the extracts to inhibit angiotensin-converting enzyme (ACE) and their hypoglycemic effects were also determined; the latter was measured by $\alpha$-amylase and glucosidase inhibition. Extraction yield, protein content, total phenol, and $\beta$-glucan in the water extracts were 55.86, 17.71, 1.89, and 21.93%, respectively. The respective values for the ethanol extracts were lower than those for water extracts. Both water and ethanol extracts showed dosedependent ACE inhibition, the effect of the former being greater. The water extract inhibited ACE activity by 95.34% at 40 mg/mL. The $IC_{50}$ values of the water extracts were 63.32 and 0.41 mg/mL for $\alpha$-amylase and glucosidase, respectively. Thus, the water extracts had a greater hypoglycemic effect than the ethanol extracts. From these results, water is a better solvent than ethanol to extract from the haesongi mushroom functional components that show ACE inhibition and have hypoglycemic effects.