• 한국연초학회지
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• 제24권2호
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• pp.94-100
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• 2002

Cyclodextrin glucanotransferase from Bacillus stearothemophilus and Bacillus macerans synthesized maltol and ethyl maltol monoglucoside, with a series of its maltooligo-glucosides by transglycosylation with dextrin as a donor, and maltol or ethyl maltol as an acceptor. The monoglucoside formed from reaction mixture of maltol or ethyl maltol by the successive actions of Bacillus stearothemophilus cyclodextrin glucanotransferase and Rhizopus glucoamylase was isolated by Diaion HP-20 column and silica gel column chromatography. The structure of the isolated monoglucoside was identified as maltol-$\alpha$-D-glucoside and ethyl maltol-$\alpha$-D-glucoside, respectively, by FAB-MS, UV, $^1$H-NMR, $^{13}$ C-NMR spectra and products by hydrolysis with acid, $\alpha$ - and $\beta$ -glucosidases.

• 한국연초학회지
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• 제25권2호
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• pp.120-127
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• 2003

Cyclodextrin glucanotransferase (CGTase) from Bacillus stearothermophilus synthesized vanillin and ethyl vanillin monoglucoside, with a series of its maltooligoglucosides by transglycosylation with dextrin as a donor, and vanillin or ethyl vanillin as an acceptor. The monoglucoside formed from reaction mixture of vanillin or ethyl vanillin by the successive actions of CGTase and Rhizopus glucoamylase was isolated by extraction with n-butanol saturated with water and silica gel column chromatography. The structure of the isolated monoglucoside was identified as vanillin- $\alpha$ -D-glucoside and ethyl vanillin- $\alpha$ -D-glucoside, respectively, by FAB-MS, UV, IR, 1H-NMR, 13C-NMR spectra and products by hydrolysis with acid, $\alpha$ - and $\beta$ -glucosidases.

• Archives of Pharmacal Research
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• 제18권3호
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• pp.203-205
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• 1995

A new $4,5,8-trihydroxy-{\alpha}-tetralone-5-O-{\beta}-D-glucoside$ along with 1,4,8-trihydroxynaphthalene1-O-{\beta}-D-glucoside$ and gallic acid were isolated from the roots of Juglans mandshurica. Their structures were elucidated on the basis of spectroscopic studies.

• Journal of Microbiology and Biotechnology
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• 제26권11호
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• pp.1933-1942
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• 2016

A novel ${\alpha}-glucoside$ hydrolase (named PspAG97A) from glycoside hydrolase family 97 (GH97) was cloned from the deep-sea bacterium Pseudoalteromonas sp. K8, which was screened from the sediment of Kongsfjorden. Sequence analysis showed that PspAG97A belonged to GH97, and shared 41% sequence identity with the characterized ${\alpha}-glucoside$ BtGH97a. PspAG97A possessed three key catalytically related glutamate residues. Mutation of the glutamate residues indicated that PspAG97A belonged to the inverting subfamily of GH97. PspAG97A showed significant reversibility against changes in salt concentration. It exhibited halophilic ability and improved thermostability in NaCl solution, with maximal activity at 1.0 M NaCl/KCl, and retained more than 80% activity at NaCl concentrations ranging from 0.8 to 2.0 M for over 50 h. Furthermore, PspAG97A hydrolyzed not only ${\alpha}-1,4-glucosidic$ linkage, but also ${\alpha}-1,6-$ and ${\alpha}-1,2-glucosidic$ linkages. Interestingly, PspAG97A possessed high catalytic efficiency for long-chain substrates with ${\alpha}-1,6-linkage$. These characteristics are clearly different from other known ${\alpha}-glucoside$ hydrolases in GH97, implying that PspAG97A is a unique ${\alpha}-glucoside$ hydrolase of GH97.

• 생약학회지
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• 제36권4호통권143호
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• pp.282-290
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• 2005

The MeOH extract of the the leaves of Salix hallaisanensis (Salicaceae) was partitioned successively with $CHCl_3$, 20% MeOH, 40% MeOH and 60% MeOH solution. From the fractions obtained, 9 compounds were isolated, $diosmetin-7-O-{\beta}-d-glucoside$ (I), $diosmetin-7-O-{\beta}-D-glucosyl-(1{\rightarrow)6)-{\beta}-d-glucoside$ (II), $diosmetin-7-O-{\beta}-d-xylosyl-(1{\rightarrow}6)-{\beta}-D-glucoside$ (III), $quercetin-3-O-{\beta}-d-galactoside$ (hyperoside) (IV), $quercetin-3-O-{\alpha}-l-rhamnosyl-(1{\rightarrow}6)-{\beta}-D-glucoside(rutin)$ (V), luteolin (VI), $luteolin-7-O-{\beta}-d-glucoside$ (VII), $kaempferol-3-O-{\alpha}-l-rhamnosyl-(1{\rightarrow}6)-{\beta}-D-glucoside$ (VIII), and (+)-catechin (IX).

• Journal of Applied Biological Chemistry
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• 제50권4호
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• pp.254-258
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• 2007

Five flavonoids, isorhamnetin 3-O-${\beta}$-D-galactosyl-4'-O-${\beta}$-D-glucoside (1), isorhamnetin 3,4'-di-O-${\beta}$-D-glucoside (2), isorhamnetin 3-O-${\beta}$-D-(6-O-${\alpha}$-L-rhamnosyl)glucosyl-4'-O-${\beta}$-D-glucoside (3), isorhamnetin 3-O-${\beta}$-D-(6-O-${\alpha}$-L-rhamnosyl)glucoside (4), and isorhamnetin 3-O-${\beta}$-D-(6-O-${\alpha}$-L-rhamnosyl) galactoside (5) were isolated from the stems of Opuntia humifusa (Raf.) Raf. and their structures were identified based on LC-MS and NMR data.

• 미생물학회지
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• 제34권3호
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• pp.137-143
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• 1998

수계에서 전분 가수분해 효소의 transglycosylation 반응을 이용하여 배당체(glycoside)를 합성하였다. Glycosyl donor인 가용성전분과 glycosyl acceptor인 benzylalcohol을 기질로 하여 ${\alpha}$-amylase에 의해 합성되는 배당체는 glucose의 1번 OH기에 benzylalcohol이 ${\alpha}$형태로 결합한 benzylalcohol-${\alpha}$-glucoside(BG)와 benzylalcohol-${\alpha}$-maltoside(BM)이었다. pH 5.0의 반응에서는 주로 BG가, pH 8.0의 반응에서는 BM만이 합성되는 특이한 반응양상을 보였다. Transglycosylation 반응의 최적조건은 가용성전분 50 mg/ml, benzylalcohol 50 mg/ml, 온도 $30-35^{\circ}C$, 효소량 10 unit/ml이었으며, 합성된 BG는 ${\alpha}$-glucodisase에 의해 glucose와 benzylalcohol로 가수분해되었으며 BM의 경우는 pH 5.0에서는 glucose와 BG로 가수분해되었으나 pH 8.0이상에서는 전혀 분해되지 않았다. BM과 구조적으로 유사한 maltotriose는 pH 5.0에서 glucose와 maltose로 가수분해 되었으나 transglycosylation반응은 거의 일어나지 않았으며 pH 8.0에서는 가수분해도 transglycosylation반응도 일어나지 않았다.

• Archives of Pharmacal Research
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• 제4권2호
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• pp.129-131
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• 1981

From the steambark of Albizzia julibrissin, 3', 4' 7-trihydroxyflavone and .alpha.-spinasteryl-D-glucoside were isolated.

• Journal of Microbiology and Biotechnology
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• 제8권6호
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• pp.710-713
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• 1998

A material with the same high performance liquid chromatography (HPLC) retention profile as authentic ascorbic acid 2-Ο-$\alpha$-glucoside (AA-2G) was detected in kimchi. This material was identified as AA-2G by testing its susceptibility to $\alpha$-glucosidase hydrolysis, the HPLC profile, and through the elementary analysis. Among several strains of bacteria isolated from fermented kimchi, four strains could produce cydodextrin glucanotransferase (CGTase) which catalyzes the transglucosylation reaction of ascorbic acid. By using starch as the glycosyl donor, AA-2G was produced as the major product through this reaction.

• 생명과학회지
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• 제27권1호
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• pp.32-37
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• 2017

Cyanidin-3-O-glucoside (C3G)는 오디와 붉은색의 과일에 풍부하게 함유되어 있으며, 항염증과 항산화 효과와 관련하여 보고되어있다. 그러나, C3G의 식후 혈당에 관한 연구 결과는 보고되지 않았다. ${\alpha}-glucosidase$ 억제제는 소장에서 탄수화물 소화의 속도를 방해함으로써 식후 고혈당을 조절한다. 본 연구에서는 C3G가 ${\alpha}$-글루코시다아제와 ${\alpha}$-아밀라아제에 미치는 억제효과 및 스트렙토조토신(STZ)이 유발하는 당뇨병 생쥐의 식후고혈당에 미치는 완화 효과를 조사하였다. ICR 마우스와 streptozothocin (STZ)으로 유도된 당뇨병 마우스에 수용성 전분(2 g/kg body weigh)으로 경구부하 후 C3G (10 mg/kg body weight) 또는 acarbose (10 mg/kg body weight)를 단독 또는 함께 투여하였다. 혈액 샘플은 꼬리에서 0, 30, 60, 120분 간격으로 채취하였다. ${\alpha}$-글루코시다아제와 ${\alpha}$-아밀라아제에 대한 C3G의 $IC_{50}$ 값은 각각 13.72와 $7.5\;{\mu}M$의 결과값을 나타내어, 양성대조군인 acarbose보다 더 효과적이었다. STZ으로 유발된 당뇨 쥐의 식후 혈당 수치는 대조군에 비해 C3G 투여시 유의적으로 더 낮았다. 게다가, C3G 투여는 당뇨병 흰쥐에서 포도당 반응에 대한 곡선하면적 감소와 관련이 있었다. 그러므로, C3G는 ${\alpha}$-글루코시다아제의 강력한 억제제이며 식이 탄수화물의 흡수를 지연시킬 수 있음을 나타낸다.