• BMB Reports
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• 제42권3호
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• pp.160-165
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• 2009

The temperate Staphylococcus aureus phage ${\Phi}11$ harbors cI and cro repressor genes similar to those of lambdoid phages. Using extremely pure ${\Phi}11$ Cro (the product of the ${\Phi}11$ cro gene) we demonstrated that this protein possesses a single domain structure, forms dimers in solution at micromolar concentrations and maintains a largely $\alpha$-helical structure even at $45^{\circ}C$. ${\Phi}11$ Cro was sensitive to thermolysin at temperatures ranging from $55-75^{\circ}C$ and began to aggregate at ${\sim}63^{\circ}C$, suggesting that the protein is moderately thermostable. Of the three homologous 15-bp operators (O1, O2, and O3) in the ${\Phi}11$ cI-cro intergenic region, ${\Phi}11$ Cro only binds efficiently to O3, which is located upstream of the cI gene. Our comparative analyses indicate that the DNA binding capacity, secondary structure and dimerization efficiency of thermostable ${\Phi}11$ Cro are distinct from those of P22 Cro and $\lambda$ Cro, the best characterized representatives of the two structurally different Cro families.

• BMB Reports
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• 제40권5호
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• pp.740-748
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• 2007

To gain insight into the structure and function of repressor proteins of bacteriophages of gram-positive bacteria, repressor of temperate Staphylococcus aureus phage ${\phi}11$ was undertaken as a model system here and purified as an N-terminal histidine-tagged variant (His-CI) by affinity chromatography. A ~19 kDa protein copurified with intact His-CI (~ 30 kDa) at low level was resulted most possibly due to partial cleavage at its Ala-Gly site. At ~10 nM and higher concentrations, His-CI forms significant amount of dimers in solution. There are two repressor binding sites in ${\phi}11$ cI-cro intergenic region and binding to two sites occurs possibly by a cooperative manner. Two sites dissected by HincII digestion were designated operators $O_L$ and $O_R$, respectively. Equilibrium binding studies indicate that His-CI binds to $O_R$ with a little more strongly than $O_L$ and binding species is probably dimeric in nature. Interestingly His-CI binding affinity reduces drastically at elevated temperatures ($32-42^{\circ}C$). Both $O_L$ and $O_R$ harbor a nearly identical inverted repeat and studies show that ${\phi}11$ repressor binds to each repeat efficiently. Additional analyses indicate that ${\phi}11$ repressor, like $\lambda$ repressor, harbors an N-terminal domain and a C-terminal domain which are separated by a hinge region. Secondary structure of ${\phi}11$ CI even nearly resembles to that of $\lambda$ phage repressor though they differ at sequence level. The putative N-terminal HTH (helix-turn-helix) motif of ${\phi}11$ repressor belongs to the HTH -XRE-family of proteins and shows significant identity to the HTH motifs of some proteins of evolutionary distant organisms but not to HTH motifs of most S. aureus phage repressors.

• 한국산업정보학회논문지
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• 제11권5호
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• pp.62-66
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• 2006

We investigate various $\Phi(t)-stability$ of comparison differential equations and we abtain necessary and/or sufficient conditions for the uniform asymptotic and exponential asymptotic stability of the nonlinear differential equation x'=f(t, x).

• 한국전기전자재료학회논문지
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• 제20권11호
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• pp.959-964
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• 2007

To investigate the influence of partial discharge(PD) distribution characteristics due to various defects on the power cable joints interface, we used the K-means clustering method. As the result of PD number(n) distribution analyzing on $\Phi-n$ graph, the phase angle($\Phi$) of cluster centroid shifted to $0^{\circ}\;and\;180^{\circ}$ increasing with applying voltage. It was confirmed that the PD quantify(q) and euclidean distance of centroid were increased with applying voltage from the centroid distribution analyzing of $\Phi-q$ plane. The dispersion degree was increased with calculated standard deviation of the $\Phi-q$ cluster centroid. The PD number and mean value on $\Phi-q$ graph were some different by electric field concentration with defect types.

• 대한수학회보
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• 제22권1호
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• pp.11-15
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• 1985

Manddelberg [9] has shown that a Clifford algebra of a free quadratic space over an arbitrary semi-local ring R in Brawer-Wall group BW(R) is determined by its rank, determinant, and Hasse invariant. In this paper, we prove a corresponding result when R is a full ring.Throughout this paper, unless otherwise specified, we assume that R is a commutative ring having 2 a unit. A quadratic space (V, B, .phi.) over R is a finitely generated projective R-module V with a symmetric bilinear mapping B: V*V.rarw.R which is non-degenerate (i.e., the natural mapping V.rarw.Ho $m_{R}$(V,R) induced by B is an isomorphism), and with a quadratic mapping .phi.: V.rarw.R such that B(x,y)=1/2(.phi.(x+y)-.phi.(x)-.phi.(y)) and .phi.(rx) = $r^{2}$.phi.(x) for all x, y in V and r in R. We denote the group of multiplicative units of R by U9R). If (V, B, .phi.) is a free rank n quadratic space over R with an orthogonal basis { $x_{1}$,.., $x_{n}$}, we will write < $a_{1}$,.., $a_{n}$> for (V, B, .phi.) where the $a_{i}$=.phi.( $x_{i}$) are in U(R), and denote the space by the table [ $a_{ij}$ ] where $a_{ij}$ =B( $x_{i}$, $x_{j}$). In the case n=2 and B( $x_{1}$, $x_{2}$)=1/2 we reserve the notation [a $a_{11}$, $a_{22}$] for the space. A commutative ring R having 2 a unit is called full [10] if for every triple $a_{1}$, $a_{2}$, $a_{3}$ of elements in R with ( $a_{1}$, $a_{2}$, $a_{3}$)=R, there is an element w in R such that $a_{1}$+ $a_{2}$w+ $a_{3}$ $w^{2}$=unit.TEX>=unit.t.t.t.

• 한국터널지하공간학회 논문집
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• 제11권2호
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• pp.117-129
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• 2009

원형수직터널에서 3차원적인 아칭효과를 고려한 토압산정을 위해 여러 연구가 수행되었고 실내시험 및 수치해석을 통해 이를 검증하였으나, 다층지반과 c-${\phi}$지반에서의 적용이 어려웠다. 본연구에서는 c-${\phi}$지반에서의 토압 산정을 위해 c-${\phi}$지반에서 적용 가능한 토압계수 산정식을 구하였으며, 기존 토압식을 수정 제안하였다. 점착력이 토압에 미치는 영향을 파악하기 위해 지반을 가정하여 각 경우별로 토압을 산정하여 비교하였으며, 다층지반에서 파괴면을 가정하는 방법으로 토압을 구하였다. 이 논문은 두 개의 연속된 논문(Companion paper)의 첫 번째로서 모델개발을 위한 이론전개를 다루고 있으며, 대형 모형실험에 의한 실증은 두 번째 논문에서 다룰 것이다.

• 전기학회논문지
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• 제63권11호
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• pp.1588-1593
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• 2014

The scott transformer changes three-phase power to two single-phase power. However, this method causes unbalance and fluctuation of voltage by the change of electric railload. Recently, the AC railway system based on $3{\Phi}/1{\Phi}$ back-to-back (BTB) voltage source converter (VSC) has been proposed to solve these problems. Meanwhile, battery energy storage system (BESS) is used to compensate voltage instantaneously in power system. In this paper, BESS application to the AC railway system based on $3{\Phi}/1{\Phi}$ BTB VSC is suggested to compensate voltage instantaneously. The application effect is shown through simulation using MATLAB/SIMULINK.

• 생물환경조절학회지
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• 제26권1호
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• pp.27-34
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• 2017

본 연구에서는 간척지에서 플라스틱 온실의 기초안전성을 평가하기 위하여 강재 나선말뚝기초를 갖는 폭 6m 연동하우스 1개동과 강재 나선말뚝기초와 파이프줄기초를 각각 적용한 폭 8.2m 단동하우스 2개동을 SPT N값이 2인 간척지 시험포장에 설치하여 플라스틱 온실의 인발량 및 침하량을 측정하였다. 또한 플라스틱 온실에 적용된 나선말뚝기초 3종(${\phi}50$, ${\phi}75$ 및 ${\phi}100$)을 인근의 간척지 시험포장에 설치하여 인발저항특성을 시험하였다. 평가 기간 중 플라스틱 온실의 수직변위 움직임이 관측되었지만 온실의 규모와 비교하여 그 변위는 무시할만한 수준이었다. 평가 기간 중의 최대 수직변위는 나선말뚝 기초를 적용한 연동하우스의 경우 +9.0mm(인발)~-11.5mm(침하), 나선말뚝기초를 적용한 단동하우스의 경우 +1.3mm~-7.7mm, 파이프줄기초를 적용한 단동하우스의 경우 +0.9mm~-11.2mm로 나타났다. 나선말뚝기초의 허용인발력은 모두 극한하중 판정기준에 의해 판단할 수 있었으며 ${\phi}50mm$, ${\phi}75$와 ${\phi}100$ 나선말뚝기초의 최종 허용인발력은 각각 0.40kN, 1.0kN과 2.5kN으로 산정할 수 있었다. 평가기간 중 나선말뚝기초를 적용한 플라스틱 온실의 기초안전성을 최종적으로 판단하기에는 다소 무리가 있다고 판단되나 강재 나선말뚝기초를 간척지 플라스틱 온실의 기초로 사용하더라도 큰 문제는 없을 것으로 판단되었다.

• 미생물학회지
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• 제23권2호
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• pp.115-121
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• 1985

Lactobacillus casei에 감염하는 독성 phage중 각 분류꾼을 대표하는 5종의 phage (J1, TK93, K1, PD 5 빛 CP 1)와 l 종의 용원 phage (${\phi}$ 1043) 의 핵산의 특성을 바교 검토하였다. 실험한 6 종의 phage는 모두 double S stranded DNA를 갖고 있였으며 J1. TK93, K1 및 ${\phi}$ 1043 DNA의 크기는 약 42Kb, PD5 와 CP1 DNA는 140K Kb정도로 서로 비슷하였다. EcoR 1으로 절단시 J1, TK93, K1, PD5, CP1 및 ${\phi}$1043은 각각 13, 13. 11, 14, 14와 12개의 절편을 갖는 특징적인 절단양식을 보여주었다. J1, TK93 및 ${\phi}$ 1043 DNA에는 cohesive end가 존재 하였고 K1, PD5 빛 CP1 DNA에는 없는 것으로 사료되었다. J1과 TK93 DNA의 제한효소 지도를 작성하여 비교 검토하였으며 이상의 결과로부터 진화적 유연관계를 검토하였다.

• 대한기계학회논문집B
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• 제26권11호
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• pp.1531-1541
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• 2002

The NOx emission characteristics of C$H_4$-Air coflow jet flames were numerically and experimentally investigated. NOx was measured using a chemiluminescent detection and calculated by the parabolic -type equation solver with a detatiled NOx chemistry. The fuel flow rate( $Q_{F}$), the diameter of mixture nozzle and the equivalence rate(Ф) were varied to discuss the EINOx of each flames at the various combustion conditions. The NOx emission index(EINOx) was introduced to quantify the NOx emission from the parametrically varied flames. The results show that Prompt EINOx increases on a logarithmic profile with increasing ${\Phi}$ and keeps nearly constant for the variation of $Q_{F}$. Thermal EINOx reaches the maximum value at around ${\Phi}$ =1.5 and then slowly decrease for ${\Phi}$ >1.5. In addition, Thermal EINOx increases with increasing $Q_{F}$, but nearly indifferent to the variation of the mixture nozzle diameter. Total EINOx also shows a peak at around ${\Phi}$ =1.5, followed by a relatively sharp decrease for 1.5< ${\Phi}$ <2.5 and increase slowly for 2.5 < ${\Phi}$ < $\infty$ The present Total EINOx trend is well explained by a combination of above Thermal and Prompt EINOx trend with the variation of ${\Phi}$ n of ${\Phi}$.