• Title/Summary/Keyword: ${\Delta}opp$ mutants

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Comparisons of growth and biofilm production with Vibrio fluvialis and mutants deficient in oligopeptide permease gene (Vibrio fluvialis의 Oligopeptide Permease Gene 결손에 의한 생육과 Biofilm 생산의 비교)

  • Lee, Eun Mi;Ahn, Sun Hee;Kong, In Soo
    • Journal of Marine Bioscience and Biotechnology
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    • v.1 no.2
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    • pp.84-90
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    • 2006

  • Various ${\Delta}opp$ mutants of Vibrio fluvialis were constructed by allelic exchange method. The mutants occurred in target genes were confirmed by PCR and Southern hybridization analyses. After the exact mutants were identified, cell growth and biofilm production were examined using the respective mutants. The growth of wild strain was more rapid than mutants within 4hr incubation. Thereafter, the growth of wild strain and mutants reached to same level. When the productivities of wild strain and mutants were examined, ${\Delta}oppA$ mutant showed the highest productivity. Though ${\Delta}oppC,D$ and F mutants produced the lower production than that of ${\Delta}oppA$ mutant, the productivities of those mutants were much higher than that of wild strain.

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