• Title/Summary/Keyword: $^14$C-sucrose

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Translocation of $^14$C-assimilates During Grain Filling and Influence of Defoliation and Emasculation on Grain Weight in Oats (연맥 등숙기 동안 $^14$C물질의 전유와 절엽 및 제영이 종실중에 미치는 영향)

  • 이호진
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.24 no.4
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    • pp.38-44
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    • 1979
  • $^14$C-sucrose was labeled on detached panicles and $^14{CO}_2$ on flag leaves or panicles of intact plants to study grain sink activity in spring oats cultivar Pennfield. Defoliation and emasculation experiment was conducted to study source-sink relationship during grain filling. Specific activity of groat rose up to 15 days after anthesis and declined rapidly to 18 days. Daily gain of groat wt. matched closely with specific activity of. groat during grain filling. Primary groats were higher in specific activity of groat than secondary groats.$^14{CO}_2$ exposure on panicle was three times higher in specific activity of groat than $^14{CO}_2$exposure on flag leaf. In the defoliation and emasculation experiment, groat wt. of Pennfield oats decreased as ratio of source/sink decreased. Half number of spikelets with half leaf area was no different in groat wt. compared to control, but normal number of spikelets with zero leaf area was decreased 16% in groat wt., indicating a significant compensation by green area on panicle.

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Studies on the Estimation of Theoretical cmc and Micellization Range by Computer Programming (컴퓨터 프로그래밍에 의한 이론적 임계미셀 농도 및 미셀화 영역의 산정에 관한 연구)

  • Kim, Tae-Young;Cho, Gyung-Heung;Choi, Seung-Ok;Jeong, No-Hee;Nam, Ki-Dae
    • Journal of the Korean Applied Science and Technology
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    • v.14 no.3
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    • pp.63-69
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    • 1997
  • Micellization range and cmc for the dilute aqueous solution of sucrose monoesters, that is, sucrose monolaurate, sucrose monomyristate, sucrose monopalmitate, sucrose monostearate and sucrose monooleate were investigated by computer programming. The extreme of the curvature of ${\sigma}$(C) as a new suggested method to determine the cmc and the micellization range was accomplished with computer programming. The values of cmc and micellization range can be obtained by the extreme of the curve by direct processing of only a few experimental data. The values of cmc particulary was in good agreement with those deduced intuitively from the shape of experimental curves of ${\sigma}$(C).

Effect of the Application of Sucrose on Rapid Decrease of Soil Inorganic Nitrogen (Sucrose 처리가 토양 무기태질소의 신속 감소에 미치는 영향)

  • Ku, Hyun-Hwoi;Lim, Woo-Jin;Lee, Sang-Eun
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.4
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    • pp.424-429
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    • 2010
  • To solve the problems with excessive accumulation of soil inorganic N and resulting saline soils from overuse of nitrogen fertilizer, the effect of sucrose application on decrease of soil inorganic N content and electrical conductivity (EC) was studied. Sucrose treatment greatly reduced ${NH_4}^+$-N content in soil. The amount of reduction was greater as the amount of sucrose treatment was increased. When ${NH_4}^+$-N content was reached the lowest point (about 10 mg $kg^{-1}$or lower), the C/N ratio, which determines the amount of sucrose treatment, was around 10 regardless of initial ${NH_4}^+$-N content. For the rate of ${NH_4}^+$-N reduction 15~36 hours was required to reduce the initial ${NH_4}^+$-N content to half, and 36~69 hours to lower ${NH_4}^+$-N content to the lowest point (about 10 mg $kg^{-1}$or lower). In addition, sucrose treatment greatly lowered ${NO_3}^-$-N content. In case of C/N ratio above 10, initial ${NO_3}^-$-N content of 348 mg $kg^{-1}$ was reduced to the lowest of 14~21 mg $kg^{-1}$. As for the rate of ${NO_3}^-$-N reduction by sucrose treatment, it took 36~60 hours for ${NO_3}^-$-N content to reach the lowest point for C/N ratio of 10 or higher, and it took 3 weeks, comparably longer time, for C/N ratio of 5. Lowering soil EC from sucrose treatment showed the same trend as ${NO_3}^-$-N content. As an important energy and carbon source for humankind, sugar should not be wasted and must be carefully applied to soil. In principle, the best way of preventing salt accumulation in soil is to optimize the fertilizer input. However, when over-fertilization should be dealt with, the sucrose treatment would be a possible and effective counter-measure to reduce overdosed nitrogen sources in soil.

Glucose Oxidation and It's Oxidative Enzyme Systems in Dunaliella tertiolecta.(I) Oxidation of 14C-glucose in Whole Cells and Cell-free Systems (Dunaliella tertiolecta의 포도당산화와 산화효소계 (I) Whole cells과 cell-free systems에 의한 14C-glucose의 산화)

  • 권영명
    • Journal of Plant Biology
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    • v.12 no.2
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    • pp.7-14
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    • 1969
  • Dunaliella tertiolecta did not show any increase in respiration rate when supplied with glucose, glycerol, sucrose, L-alanine, acetate, pyruvate and succinate. This was in contrast to Chlorella pyrenoidosa, which, under identical conditions, showed significant increase when supplied with glucose or acetate but not with the other compounds. Production of 14CO2 from added 14C-glucose in D. tertiolecta was lower than the other 14C-labelled substrates: L-alinine, glycerol, succinate, but higher than 14C-sucrose addition. And it was also lower than C. pyrenoidosa experiments which was added 14C-glucose as a substrate. Light reduced amounts of labelled carbon dioxide from 14C-glucose or 14C-acetate and increased incorporation of 14C from the substrates to cell materials in either D. tertiolecta or C. pyrenoidosa. The contribution of 14C from 14C-glucose to 14CO2 in cell-free system of D. tertiolecta were much higher than in whole cell suspension. It was contrast to C. pyrenoidosa which were showed reduction of 14CO2 production in cell-free systems than whole cell suspensions. When cell-free systems of D. tertiolecta and C. pyrenoidosa were supplied with ATP, NAD, NADP or/and hexokinase, it was remarkably increased production of 14CO2 from the substrates than the control. It was concluded that the low ability of D. tertiolecta to metabolize glucose were caused by the impermeability of the cell membrane to glucose and were not due to deficiencies of enzyme systems concerning glucose metabolism. In the cell-free systems, it seemed to be more active pentose phosphate pathway than glycolytic pathway in D. tertiolecta.

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Synthesis of Fine Ba-Nd-Ti-O Powders by Spray Pyrolysis from Spray Solution with Sucrose (수크로스 함유 분무용액으로부터 분무열분해 공정에 의한 미세 Ba-Nd-Ti-O 분말 합성)

  • Ko, You-Na;Jung, Dae-Soo;Koo, Hye-Young;Kang, Yun-Chan
    • Korean Journal of Materials Research
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    • v.20 no.3
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    • pp.142-147
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    • 2010
  • Nano-sized $BaNd_2Ti_5O_{14}$ powders were prepared by the spray pyrolysis process. Sucrose used as the organic additive enabled the formation of nano-sized $BaNd_2Ti_5O_{14}$ powders. The powders prepared from the spray solution without sucrose had a spherical shape, dense structure and micron size before and after calcination. However, the precursor powders prepared from the spray solution with sucrose had a large size, and hollow and porous morphology. The precursor powders had an amorphous crystal structure because of the short residence time of the powders inside the hot wall reactor. The complete decomposition of sucrose did not occur inside the hot wall reactor. Therefore, the precursor powders obtained from the spray solution with sucrose of 0.5M had a carbon content of 39.2wt.%. The powders obtained from the spray solution with sucrose of 0.5M had a slightly aggregated structure of nano-sized primary powders of $BaNd_2Ti_5O_{14}$ crystalline phase after calcination at $1000^{\circ}C$. The calcined powders turned into nano-sized $BaNd_2Ti_5O_{14}$ powders after milling. The mean size of the $BaNd_2Ti_5O_{14}$ powders was 125 nm.

Quality Evaluation of Pumpkin Jam Replaced Sucrose with Sugar Alcohols during Storage (당알콜 첨가 호박잼 저장 중 품질 특성)

  • 이근종;김미리
    • Journal of the East Asian Society of Dietary Life
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    • v.14 no.2
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    • pp.123-130
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    • 2004
  • The physicochemical and sensory qualities of pumpkin jams replaced sucrose with sugar alcohols were investigated during storage at 2$0^{\circ}C$. Pumpkin jam was prepared with steamed ground pumpkin, mixed with sucrose only(50%), sorbitol (sucrose 30%+sorbitol 20%) or maltitol (sucrose 30%+maltitol 20%). Final sweetness of each pumpkin jam was 64$^{\circ}$ Brix. During 60 days of storage there were no differences in acidity and pH among treatments. Reducing sugar content was higher in sucrose, compared to maltitol or sorbitol. During storage, Hunter L, a and b values increased; L and b values were the highest in maltitol and a value were the highest in sucrose compared to the other sugars. Adhesiveness and hardness of textural properties were the highest in sugar and the lowest in maltitol. Sensory evaluation results showed that the mean scores of color, clarity, flavor and overall acceptability were the highest in maltitol, compared to sucrose or sorbitol. Physicochemical and sensory characteristics of pumpkin jams during storage in a PCA plot comprised of first principal component (58.79%) and second principal component (20.94%).

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Purification and Characterization of Invertase from Korean Ginseng Panax ginseng (고려인삼(Panax ginseng) Invertase의 정제와 그 특성)

  • 김용환;김병묵
    • Journal of Ginseng Research
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    • v.14 no.1
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    • pp.14-20
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    • 1990
  • In An invertase (EC 3.2.1.26) was extracted from Korean giseng (Panax ginseng C.A. Meyer) with distilled tvater The ginseng invertase was purified about 62.6 folds purified by procedures including ammonium sulfate fractionation , DEAE-cellulofine chromatography and gelfiltrations through Sephadex G-75 and the recovery of enzyme activity was 11.1%. The homogeneity of the purified enzyme was probed by polyacrylamide gel disc electrophoresis. The purifled enzyme was divided into two different subunits by treating with a mixture of SDS and 2-mercautoethanol, and the molecular weight of the large subunit was estimatedtobe 116,000 and that of the small one to be 14,000. The optimal VH and temperature of the enzyme were pH 6 and 45$^{\circ}C$, respectively. The enzyme hydrolyzed specifically the hydrolyzation of the -fructofuranosides such as sucrose, raffinose and inulin. The Km values of the enzyme for sucrose and raffinose were determined to be 0.85 and 0.6 mM, respectively.

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Physicochemical Properties of a Low Calorie Sweetener, Tagatose (저열량 감미료 Tagatose의 이화학적 특성)

  • Roh, Hoe-Jin;Kim, Sang-Yong;Kim, Suk-Shin;Oh, Deok-Kun;Han, Kee-Young;Noh, Bong-Soo
    • Korean Journal of Food Science and Technology
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    • v.31 no.1
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    • pp.24-29
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    • 1999
  • The physicochemical properties of tagatose, a low calorie sweetener, was investigated. Rheological property of tagatose solution was found to be Bingham fluid. As the concentration of tagatose increased from 10 to 50% at $25^{\circ}C$, the viscosity increased from 1.65 to 5.14 cp. When the temperature of 40% tagatose solution increased from 15 to $55^{\circ}C$, the viscosity decreased from 4.59 to 2.33cp. The melting onset temperature and endothermic enthalpy of tagatose were $130.4^{\circ}C$ and -202.3 J/g, respectively, which were obtained from the analysis of differential scanning calorimetry. Tagatose showed higher water absorption than sucrose under $85{\sim}100%$ of relative humidity. Tagatose was less soluble than sucrose at $20{\sim}70^{\circ}C$. Water activity of tagatose in 60% concentration was 0.892, which was lower than 0.957 of sucrose solution. Tagatose solution adjusted from pH 2 to pH 12 was stable after 3 days. Amount of tagatose was not changed after heat treatment at $154^{\circ}C$ for 4 hours. But a browning reaction was found and absorbance of a tagatose solution increased with heat treatment.

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Optimum Lactose: Sucrose Ratio for the Pigs Weaned at 21 Days of Age

  • Jin, C.F.;Kim, J.H.;Han, In K.;Yeon, J.U.
    • Asian-Australasian Journal of Animal Sciences
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    • v.11 no.2
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    • pp.185-191
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    • 1998
  • A total of 240 weaned pigs (BW 5.4 kg) were allotted in a completely randomized block design to find out the optimum lactose : sucrose ratio for the weaned pigs. Treatments were 100:0 (A), 75:25 (B), 50:50 (C), 25:75 (D) and 0:100 (E) in lactose : sucrose ratio. For the first week after weaning, average daily gain (ADG) and feed/gain (F/G) were improved (p < 0.05) for pigs fed A, B and C diets compared to pigs fed diets D and E, but the average daily feed intakes (ADFI) of the pigs were not different. From d 7 to 14, no significant differences was found in ADG and ADFI among the treatments, but pigs fed the diets A, B and C had improved F/G compared with the pigs fed diets D and E (p < 0.05). From d 15 to 21, no significant differences were found in ADG, ADFI and F/G among the treatments. The digestibility of nutrients was not influenced by the lactose : sucrose ratio except nitrogen. Nitrogen digestibility of A diet was significantly higher than D and E diets (p < 0.05) but no significant differences were found among diets A, B and C. Dry matter and phosphorus excretions were not influenced by the treatments. The pigs fed diets A, B and C excreted significantly less nitrogen than the pigs fed diets D and E. In conclusion, the results suggest that sucrose can effectively replace up to 50% lactose in starter diets.

Effect of Sugars on Thermal Gelation of Surimi Sols

  • Lim, Seung-Taik;Lee, Young-Seung;Yoo, Byoung-Seung
    • Food Science and Biotechnology
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    • v.14 no.3
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    • pp.340-343
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    • 2005
  • Surimi samples were prepared with the addition of three different sugars (sucrose, glucose, and fructose) at 8% and the thermal gelation of surimi sols was investigated by small-deformation oscillatory measurements of storage (G') and loss (G") moduli. The magnitudes of G' at $10^{\circ}C$ were much greater than G" over the entire range of frequency (${\omega}$), with little dependence on ${\omega}$. In general, G' values of surimi sol containing sucrose during heating (from 10 to $95^{\circ}C$) was pronounced than those of glucose and fructose, showing the following order: sucrose>glucose>fructose. The transition peaks of surimi sols containing sugars were in the temperature range of $34.8-37.4^{\circ}C$.