• Asian-Australasian Journal of Animal Sciences
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• v.16 no.1
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• pp.15-22
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• 2003

Experiments were conducted to evaluate the effect of blastomere diameters and cell cycle stages on the subsequent development of nuclear transplant rabbit embryos (NT-embryos) using nuclei derived from the 16- or 32-cell stage embryos. All blastomeres and NT-embryos were cultured individually in modified Ham's F-10 medium supplemented with 10% rabbit serum (RS) at $38^{\circ}C$ and 5% $CO_2$ in air. The diameter of blastomeres from 16-cell stage embryos was found twice of those from 32-cell stage (51 vs 27 ${\mu}m$). Significant differences were observed in cleavage rates ($\geq$3 divisions) in the isolated single blastomeres (54 vs 48 for 16-cell; 28 vs 14 for 32-cell, p<0.05), but the fusion rates of oocytes with transferred nuclei were similar between small and large single blastomeres derived from either 16-cell or 32-cell stage embryos. When 16-cell stage blastomeres were used as nuclear donors, cleavage rates ($\geq$3 divisions) of the NT-embryos were greater in the small nuclear donors than in the large donors (73 vs 55%, p<0.05). On the contrary, significantly higher cleavage (43 vs 6%, p<0.05) and developmental rates (14 vs 0%, p<0.05) were observed in the large blastomere nuclear donor group of the 32-cell stage embryos. When the cell cycle stages were controlled by a microtubule polymerization inhibitor (Demicolcine, DEM) or the combined treatment of DEM and Aphidicolin (APH), a DNA polymerase inhibitor, fusion rates were 88-96% for the 16-cell donor group (without DEM treatment), which were greater than the 32-cell donor group (54-58%). Cleavage rates were also greater in the transplants derived from G1 nuclear donor group (93-95%) than those from the DEM and APH combined treatment (73%) for the 16-cell donor group (p<0.05). No significant difference was detected in the morula/blastocyst rates in either donor cell stage (p>0.05). In conclusion, it appeared that no difference in the developmental competence between large and small isolated blastomeres was observed. When smaller 16-cell stage blastomeres were used as nuclear donor, the cleavage rate or development of NT-embryos was improved and was compromised when 32-cell stage blastomeres were used. Therefore, control nuclear stage of the donor cell at $G_1$ phase in preactivated nuclear recipients seemed to be beneficial for the cleavage rate of the reconstructed embryo in the 16-cell transplant, but not for subsequent morula or blastocyst development.

• Journal of the Korean Orthopaedic Association
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• v.56 no.5
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• pp.391-397
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• 2021

Purpose: Tranexamic acid (TXA) can reduce perioperative blood loss and the frequency of blood transfusions in lower extremity surgery. On the other hand, the effects of TXA on reverse total shoulder arthroplasty (rTSA) remain undetermined. This study evaluated the efficacy of TXA on perioperative blood loss, transfusion requirements, and the change in the hemotologic index. Materials and Methods: This study evaluated patients who underwent rTSA from September 2009 to July 2020. The patients were classified into two groups. The TXA group were administered TXA intravenously and topical TXA during surgery. The non-TXA group was not administered TXA. The quantity of hemovac drainage, which represented the postoperative blood loss, transfusion requirements, and postoperative change in hemoglobin and hematocrit level, were recorded. Results: The TXA and non-TXA groups consisted of 93 and 84 patients, respectively. The preoperative demographics showed no significant differences in age (72.0±7.0 vs. 71.5±5.8, p=0.656), sex (male:female, 28:65 vs. 23:61, p=0.689) and the prevalence of hypertension and diabetes (hypertension:diabetes:both, 36:3:13 vs. 32:3:8, p=0.806) between the two groups. There were significant differences in the requirements of transfusion (0 vs. 9, p=0.001), hemovac drainage at the 1^st (98.8±61.2 ml vs. 162.7±98.8 ml, p<0.001), the 2^nd postoperative day (73.8±48.4 ml vs. 91.5±54.5 ml, p=0.024), hemoglobin level at the 1^st (11.7±1.2 g/dl vs. 11.2±1.4 g/dl, p=0.048), 3^rd (10.9±1.2 g/dl vs. 10.2±1.2 g/dl, p<0.001), and 6^th (11.2±1.3 g/dl vs. 10.7±1.3 g/dl, p=0.020) postoperative day, and the hematocrit level at the 1^st (35.0%±3.6% vs. 32.5%±3.8%, p=0.001), 3^rd (32.3%±5.0% vs. 29.8%±3.6%, p<0.001), and 6^th (33.5%±3.8% vs. 31.5%±3.7%, p<0.001) postoperative day between the two groups. Conclusion: Intravenous and topical intra-articular TXA can reduce the transfusion requirement and blood loss in rTSA.

• The Journal of Korean Physical Therapy
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• v.12 no.2
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• pp.185-190
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• 2000

Objectives: The purpose of this study was to compare the difference of Lumbar Lordosis Angle(LLA) between patients with Low Back Pain(LBP) and control groups. Methods: Questionnaires were completed by 40 adult LBP patients seeking physical therapy services and by 40 controls at the Department of Physical Therapy. Saejong Neurosurgical Clinic in Taegu city from October 1999 to March 2000. LLA was measured on lateral x-ray films in a standing position. The angle between a line parallel to the top of the first Lumbar(L1) and the top of the fifth Lumbar(L5) was defined LLA. Results: LLA of $29.88^{\circ}$ for LBP patients was a statistically significant decrease from that of $35.31^{\circ}$ for controls in the difference of lumbar lordosis(p<0.01). There were statistically significant differences between senders in patient groups. Females$(32.32^{\circ})$ had significantly greater angles than males$(27.32^{\circ})$(p<0.05), while $36.63^{\curc}$ for female was also greater than $34.12^{\circ}$ for male in the controls. No significant difference was found between age. In patient groups, $27.95^{\circ}$ for below age 40 was a smaller than $32.32^{\circ}$ for above, however, $35.82^{\circ}$ for below age 40 was a little greater than $34.27^{\circ}$ for above in controls. Patients in a sitting posture had greater LLA$(31.35^{\circ}$ than those standing$(28.93^{\circ})$, however values for controls were similar to each other. Conclusion: Results from this study indicate that distinct differences exist among patients and controls and gender, whereas little difference exists in age and working posture.

• Korean journal of applied entomology
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• v.57 no.2
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• pp.77-85
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• 2018

We surveyed 281 sites of tropical plants and 666 sites of fruit plants for three years (2015~2017) on Pseudococcus longispinus and Pseudococcus orchidicola which have not been surveyed domestically. In tropical plants, P. longispinus were found at 34 sites, while P. orchidicola were found at 87 sites. However, both species were not found in fruit plants. The developmental characteristics of P. longispinus and P. orchidicola were investigated under various temperatures. The female nymph of P. longispinus did not develop at $14^{\circ}C$ and the developmental period was the longest at $16^{\circ}C$ for 361.4 days and the shortest at $32^{\circ}C$ for 39.0 days. The longevity of female adult of P. longispinus was the shortest at $28^{\circ}C$ as 71.7 days. The number of offspring was highest at 177.7 at $32^{\circ}C$. The female nymph of P. orchidicola did not develop at $12^{\circ}C$. However, the developmental period was the longest at $14^{\circ}C$ for 184.9 days and the shortest at $28^{\circ}C$ for 21.5 days. The longevity of female adult of P. orchidicola was the shortest at 51.5 days at $28^{\circ}C$. The number of offspring was highest at 143.8 at $28^{\circ}C$. The net reproductive rate ($R_0$) and intrinsic rate of increase ($r_m$) of P. longispinus were 162.3 and 0.127 at $32^{\circ}C$, respectively. The $R_0$ and $r_m$ of P. orchidicola were 98.3 and 0.139 at $28^{\circ}C$, respectively. These results suggest that the optimum temperature of P. longispinus and P. orchidicola was $32^{\circ}C$ and $28^{\circ}C$, respectively. Therefore, we guess that they can never be able to survive the winter of Korea.

• The KIPS Transactions:PartA
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• v.12A no.2 s.92
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• pp.127-136
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• 2005

This paper measured a proper ratio of the size of demand fetch cache $L_1$ to that of prefetch cache $L_P$ by imulation when the size of $L_1$ and $L_P$ are constant which organize space-limited level 1 cache of a single microprocessor chip. The analysis of our experiment showed that in the condition of the sum of the size of $L_1$ and $L_P$ are 16 KB, the level 1 cache organization by constituting $L_P$ with 4 KB and employing OBL and FIFO as a prefetch technique and a cache replacement policy respectively resulted in the best performance. Also, this analysis showed that in the condition of the sum of the size of $L_1$ and $L_P$ are over 32 KB, employing dynamic filtering as prefetch technique of $L_P$ are more advantageous and splitting level 1 cache by constituting $L_1$ with 28 KB and $L_P$ with 4 KB in the case of 32 KB of space are available, by constituting $L_1$ with 48 KB and $L_P$ with 16 KB in the case of 64 KB elicited the best performance.

• Environmental Analysis Health and Toxicology
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• v.9 no.1_2
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• pp.37-51
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• 1994

To evaluate the genotoxicities of workers exposed to glue and glue cleaning solution, ambient air monitoring of working place, animal study and human monitoring were carried out. By GC-MS analysis, air samples collected from shoesmaking plant were found to be toluene, xylene, cyclohexane, n-hexane, methyl ethyl ketone, trichloroethylene, butylacetate, isopropyl alcohol. Glue and glue cleaning solution from shoesmaking plant were applicated topically to the CD-1 mice. DNA was isolated from skin 24 hr following the application and analysed for DNA-adducts using the nuclease $P_1$version of $^{32}$P-postlabelling assay. RAL (Relative Adduct Labelling, adducts$10^8$ nucleotides) was significantly increased in a dose-dependent manner in the glue cleaning solution treated mice skin. Peripheral blood DNA-adducts of workers exposed to glue and glue cleaning solution were also analysed by the same method, but there were not significant differences in the peripheral blood DNA-adducts level between exposed and control workers. In addition, glue cleaning solution from shoes factory was evaluated for mutagenicity in the Salmonella plate incorporation assay using strains TA 100 and TA 1535 in the presence and absence of Arochlor 1254-induced rat liver S$_{9}$. There was evident mutagenicity for cleaning solution in TA 100 regardless of $S_9$, but TA 1535 showed positive only in the absence of $S_9$when predicted by Stead model of mutagenicity prediction (p=0.0000). The urine concentrates from workers and controls were also assayed for mutagenicity towards strain TA 100 of Salmonella typhimurium in the presence of $S_9$ using Kado's microsuspension assay, but their mutagenic activities were not found to be significant. These data suggest that shoesmaking workers are exposed to genotoxic compounds and need to be monitored by testing the mutagenicity of human urines. However, $^{32}$P-postlabelling application requires further validation for the routine monitoring of human exposure.osure.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.12
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• pp.7433-7438
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• 2013

Aim: To identify new biomarkers for NPC diagnosis with an anti-EBV Western blot test kit. Methods: Serum samples from 64 NPC patients and healthy subjects with four specific VCA-IgA/EA-IgA profiles were tested with an anti-EBV Western blot test kit from EUROIMMUN AG. Proteins were quantified with scores of intensity visually assigned to the protein bands. The markers which showed statistical differences between the NPC and non-NPC subjects were further evaluated in another 32 NPC patients and 32 controls in comparison with established biomarkers including VCA-IgA, EA-IgA, EBV-related protein IgG, and EBV DNA. Results: Among the markers screened, EA-D p45-IgG showed a statistically significant difference (p < 0.05) between NPC and non-NPC subjects with VCA-IgA positivy. In 32 VCA-IgA positive NPC patients and 32 control subjects, the diagnostic accuracy of EA-D p45-IgG was 78.1% with a positive predictive value of 77.8% and a negative predictive value of 78.6%. In the verification experiment, the specificity and sensitivity of EA-D p45-IgG were 75.0% and 90.6 %, respectively. Conclusions: EA-D p45-IgG might be a potential biomarker for NPC diagnosis, especially among VCA-IgA positive subjects.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11b
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• pp.61-61
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• 2002

This study was excuted to decide the physiological characteristics of Kuehneromyces mutabilis. K. mutabilis was tested to select pertinent substract, temperature and pH range for the growth. Mycelial growth of K. mutabilis was mostly supported on MYPA among other tested synthetic or semi-synthetic media. The temperature range for pertinent mycelial growth was about 25~32$^{\circ}C$ and mostly stimulated at $25^{\circ}C$. And the pertinent pH range of MYPA was 5.0~6.0. The required carbon and nitrogen source for mycelial growth of K. mutabilis was tested. The mycelial growth was mostly stimulated by soluble starch at content. The carbon sources for pertinent mycelial growth was mannose or glucose. And the nitrogen source for pertinent mycelial growth was yeast extract or peptone.

• Journal of the Korea Convergence Society
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• v.12 no.9
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• pp.361-371
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• 2021

This study aims to identify the moderating effect of patient activation in relation between trust in healthcare professionals and shared decision making in patients with diabetes mellitus. The participants were 186 patients who received treatment for diabetes at the department of endocrinology in the tertiary hospital located in Daejeon and consented to participate in our survey. The results of this study were a significant interaction between trust in healthcare professionals(β=0.32, p=.045) and patient activation(β=0.32, p=.024) was associated with shared decision making. The patient activation had a moderating effect between trust in healthcare professionals and shared decision making in patients with diabetes mellitus(β=0.25, p=.019). Therefore, it is necessary to assess trust in healthcare professionals to improve negative awareness of healthcare professionals and strengthen trust in them. In addition, to promote participation in shared decision making, it is necessary to develop a convergent program to focus patient activation.

• The Journal of the Convergence on Culture Technology
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• v.9 no.3
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• pp.111-119
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• 2023

Purpose: This is a descriptive survey study for identifying the factors affecting the communication skills of nursing students in the current situation where non-face-to-face culture becomes commonplace due to the COVID-19. Methods: The data was collected from June 1-30, 2022, targeting total 160 nursing students(3rd/4th year) of three universities in Daejeon and Chungnam. Using the IBM SPSS/Win 25.0, the collected data was analyzed through the descriptive statistics, t-test, ANOVA, Pearson correlation, and multiple regression analysis. Results: In the results, the communication skills showed positive correlations with the acceptance of others (r=.32, p<.001), positive emotions in friendship (r=.44, p<.001), and empathy (r=.38, p<.001), and these variables explained total 32.0% of communication skills. Conclusion: Based on such results, it would be necessary to develop various extracurricular programs and also to establish the application measures for maintaining positive friendships and enhancing empathy.